Restriction map for
pF1KB9834
(Circular) (Six-base) MAPSORT of: pF1KB9834.seq Check: 1156 from: 1 to: 4151
pF1KB9834 4151 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 273 273
Size: 4151
AatII G_ACGT'C
Cuts at: 1055 1055
Size: 4151
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1976 2408 3155 3423 733
Size: 1243 432 747 268 1461
Fragments arranged by size:
1461 1243 747 432 268
AfeI AGC'GCT
Cuts at: 1063 1063
Size: 4151
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 303 1456 3023 303
Size: 1153 1567 1431
Fragments arranged by size:
1567 1431 1153
ApaI G_GGCC'C
Cuts at: 237 237
Size: 4151
ApaLI G'TGCA_C
Cuts at: 381 700 2921 381
Size: 319 2221 1611
Fragments arranged by size:
2221 1611 319
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 230 1033 1171 230
Size: 803 138 3210
Fragments arranged by size:
3210 803 138
AvrII C'CTAG_G
Cuts at: 507 507
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 565 936 1167 1581 1829 1864 565
Size: 371 231 414 248 35 2852
Fragments arranged by size:
2852 414 371 248 231 35
BanII G_rGCy'C
Cuts at: 237 428 857 1165 2195 237
Size: 191 429 308 1030 2193
Fragments arranged by size:
2193 1030 429 308 191
BbeI G_GCGC'C
Cuts at: 569 940 1585 1833 569
Size: 371 645 248 2887
Fragments arranged by size:
2887 645 371 248
BbsI GAAGACnn'nnnn_
Cuts at: 1008 1008
Size: 4151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4117
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 731 1671 731
Size: 940 3211
BglI GCCn_nnn'nGGC
Cuts at: 252 252
Size: 4151
BglII A'GATC_T
Cuts at: 789 1666 3703 789
Size: 877 2037 1237
Fragments arranged by size:
2037 1237 877
BlpI GC'TnA_GC
Cuts at: 422 1263 422
Size: 841 3310
Bme1580I G_kGCm'C
Cuts at: 237 385 704 1776 1869 2925 237
Size: 148 319 1072 93 1056 1463
Fragments arranged by size:
1463 1072 1056 319 148 93
BmrI ACTGGGnnnn_n'
Cuts at: 285 1363 1444 1779 3923 285
Size: 1078 81 335 2144 513
Fragments arranged by size:
2144 1078 513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 635 1089 332
Size: 303 454 3394
Fragments arranged by size:
3394 454 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1327 2206 2698 2996 628
Size: 699 879 492 298 1783
Fragments arranged by size:
1783 879 699 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1065 3855 1065
Size: 2790 1361
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 566 877 937 1052 1582 1830 2532 4028 566
Size: 311 60 115 530 248 702 1496 689
Fragments arranged by size:
1496 702 689 530 311 248 115 60
BsaWI w'CCGG_w
Cuts at: 140 1340 1861 2539 2813 2960 3371 140
Size: 1200 521 678 274 147 411 920
Fragments arranged by size:
1200 920 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 271 301 857 887 271
Size: 30 556 30 3535
Fragments arranged by size:
3535 556 30 30
BseYI C'CCAG_C
Cuts at: 303 1075 1576 2334 2911 3407 303
Size: 772 501 758 577 496 1047
Fragments arranged by size:
1047 772 758 577 501 496
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 268 385 428 704 857 1165 1943 2133 2925
Size: 117 43 276 153 308 778 190 792
Cuts at: 2925 268
Size: 1494
Fragments arranged by size:
1494 792 778 308 276 190 153 117
43
Bsp1286I G_dGCh'C
Cuts at: 237 268 385 428 704 857 1165 1776 1869
Size: 31 117 43 276 153 308 611 93
Cuts at: 1869 1943 2133 2195 2925 237
Size: 74 190 62 730 1463
Fragments arranged by size:
1463 730 611 308 276 190 153 117
93 74 62 43 31
BspEI T'CCGG_A
Cuts at: 140 3371 140
Size: 3231 920
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 207 273 998 1201 1415 1717 2098 207
Size: 66 725 203 214 302 381 2260
Fragments arranged by size:
2260 725 381 302 214 203 66
BsrBI CCG'CTC
Cuts at: 50 954 2443 2497 3981 4146 50
Size: 904 1489 54 1484 165 55
Fragments arranged by size:
1489 1484 904 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 251 571 2149 2330 2539 251
Size: 320 1578 181 209 1863
Fragments arranged by size:
1863 1578 320 209 181
BsrGI T'GTAC_A
Cuts at: 329 329
Size: 4151
BssHII G'CGCG_C
Cuts at: 1097 1097
Size: 4151
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 522 522
Size: 4151
BstYI r'GATC_y
Cuts at: 1 789 1116 1176 1643 1666 2001 2247 3259
Size: 788 327 60 467 23 335 246 1012
Cuts at: 3259 3345 3357 3703 1
Size: 86 12 346 449
Fragments arranged by size:
1012 788 467 449 346 335 327 246
86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 581 581
Size: 4151
BtgI C'CryG_G
Cuts at: 238 345 238
Size: 107 4044
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 603 1394 603
Size: 791 3360
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 359 573 1736 1910 2301 2328 3673 359
Size: 214 1163 174 391 27 1345 837
Fragments arranged by size:
1345 1163 837 391 214 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 230 682 2174 2384 230
Size: 452 1492 210 1997
Fragments arranged by size:
1997 1492 452 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 423 2359 2669 2815 3961 423
Size: 1936 310 146 1146 613
Fragments arranged by size:
1936 1146 613 310 146
EcoICRI GAG'CTC
Cuts at: 426 855 1163 426
Size: 429 308 3414
Fragments arranged by size:
3414 429 308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 635 733 1089 1976 2408 3155 3423 332
Size: 303 98 356 887 432 747 268 1060
Fragments arranged by size:
1060 887 747 432 356 303 268 98
EcoNI CCTnn'n_nnAGG
Cuts at: 824 824
Size: 4151
EcoO109I rG'GnC_Cy
Cuts at: 234 1290 234
Size: 1056 3095
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 569 940 1044 1065 1585 1833 2855 3834 569
Size: 371 104 21 520 248 1022 979 886
Fragments arranged by size:
1022 979 886 520 371 248 104 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1108 1140 1993 2023 2025 2055 1108
Size: 32 853 30 2 30 3204
Fragments arranged by size:
3204 853 32 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 565 936 1581 1829 565
Size: 371 645 248 2887
Fragments arranged by size:
2887 645 371 248
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 764 2822 3006 764
Size: 2058 184 1909
Fragments arranged by size:
2058 1909 184
MscI TGG'CCA
Cuts at: 361 1912 361
Size: 1551 2600
MslI CAynn'nnrTG
Cuts at: 520 2140 2267 520
Size: 1620 127 2404
Fragments arranged by size:
2404 1620 127
MspA1I CmG'CkG
Cuts at: 240 347 1009 1262 1576 1936 2949 3194 240
Size: 107 662 253 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 662 360 314 253 245 107
NaeI GCC'GGC
Cuts at: 253 573 2332 253
Size: 320 1759 2072
Fragments arranged by size:
2072 1759 320
NarI GG'CG_CC
Cuts at: 566 937 1582 1830 566
Size: 371 645 248 2887
Fragments arranged by size:
2887 645 371 248
NgoMIV G'CCGG_C
Cuts at: 251 571 2330 251
Size: 320 1759 2072
Fragments arranged by size:
2072 1759 320
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 275 275
Size: 4151
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PspOMI G'GGCC_C
Cuts at: 233 233
Size: 4151
PstI C_TGCA'G
Cuts at: 181 299 1198 1883 181
Size: 118 899 685 2449
Fragments arranged by size:
2449 899 685 118
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 428 857 1165 428
Size: 429 308 3414
Fragments arranged by size:
3414 429 308
SacII CC_GC'GG
Cuts at: 241 348 241
Size: 107 4044
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 682 2174 2384 682
Size: 1492 210 2449
Fragments arranged by size:
2449 1492 210
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 649 3668 649
Size: 3019 1132
SfcI C'TryA_G
Cuts at: 33 177 295 1194 1879 2872 3063 33
Size: 144 118 899 685 993 191 1121
Fragments arranged by size:
1121 993 899 685 191 144 118
SfoI GGC'GCC
Cuts at: 567 938 1583 1831 567
Size: 371 645 248 2887
Fragments arranged by size:
2887 645 371 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1035 1173 1035
Size: 138 4013
SmlI C'TyrA_G
Cuts at: 643 1306 2221 2713 2975 643
Size: 663 915 492 262 1819
Fragments arranged by size:
1819 915 663 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
StyI C'CwwG_G
Cuts at: 507 1044 1285 1639 507
Size: 537 241 354 3019
Fragments arranged by size:
3019 537 354 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 853 1734 1867 2326 2535 853
Size: 881 133 459 209 2469
Fragments arranged by size:
2469 881 459 209 133
TatI w'GTAC_w
Cuts at: 329 647 3666 329
Size: 318 3019 814
Fragments arranged by size:
3019 814 318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 271 303 857 889 3668 3700 271
Size: 32 554 32 2779 32 722
Fragments arranged by size:
2779 722 554 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 559 1646 559
Size: 1087 3064
XmaI C'CCGG_G
Cuts at: 1033 1171 1033
Size: 138 4013
ZraI GAC'GTC
Cuts at: 1053 1053
Size: 4151
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI BaeI BbvCI
BfrBI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI