Restriction map for
pF1KB9835
(Circular) (Six-base) MAPSORT of: pF1KB9835.seq Check: 1907 from: 1 to: 4163
pF1KB9835 4163 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 180 180
Size: 4163
AccI GT'mk_AC
Cuts at: 335 1201 335
Size: 866 3297
Acc65I G'GTAC_C
Cuts at: 1179 1179
Size: 4163
AclI AA'CG_TT
Cuts at: 4003 4003
Size: 4163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1988 2420 3167 3435 475
Size: 1513 432 747 268 1203
Fragments arranged by size:
1513 1203 747 432 268
AflIII A'CryG_T
Cuts at: 1515 2619 3596 1515
Size: 1104 977 2082
Fragments arranged by size:
2082 1104 977
AgeI A'CCGG_T
Cuts at: 2551 2551
Size: 4163
AleI CACnn'nnGTG
Cuts at: 132 132
Size: 4163
AlwNI CAG_nnn'CTG
Cuts at: 194 1468 3035 194
Size: 1274 1567 1322
Fragments arranged by size:
1567 1322 1274
ApaI G_GGCC'C
Cuts at: 966 966
Size: 4163
ApaLI G'TGCA_C
Cuts at: 2933 2933
Size: 4163
ApoI r'AATT_y
Cuts at: 153 1038 1167 3516 3772 153
Size: 885 129 2349 256 544
Fragments arranged by size:
2349 885 544 256 129
AseI AT'TA_AT
Cuts at: 20 20
Size: 4163
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
AvaI C'yCGr_G
Cuts at: 1183 1183
Size: 4163
BamHI G'GATC_C
Cuts at: 716 1188 716
Size: 472 3691
BanI G'GyrC_C
Cuts at: 1179 1593 1841 1876 1179
Size: 414 248 35 3466
Fragments arranged by size:
3466 414 248 35
BanII G_rGCy'C
Cuts at: 966 1177 2207 966
Size: 211 1030 2922
Fragments arranged by size:
2922 1030 211
BbeI G_GCGC'C
Cuts at: 1597 1845 1597
Size: 248 3915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 936 970 936
Size: 34 4129
BciVI GTATCCnnnnn_n'
Cuts at: 2061 2822 4163 2061
Size: 761 1341 2061
Fragments arranged by size:
2061 1341 761
BclI T'GATC_A
Cuts at: 233 1683 233
Size: 1450 2713
BfrBI ATG'CAT
Cuts at: 1074 1074
Size: 4163
BglII A'GATC_T
Cuts at: 1678 3715 1678
Size: 2037 2126
BlpI GC'TnA_GC
Cuts at: 262 1275 262
Size: 1013 3150
Bme1580I G_kGCm'C
Cuts at: 966 1788 1881 2937 966
Size: 822 93 1056 2192
Fragments arranged by size:
2192 1056 822 93
BmgBI CAC'GTC
Cuts at: 1105 1105
Size: 4163
BmrI ACTGGGnnnn_n'
Cuts at: 1375 1456 1791 3935 1375
Size: 81 335 2144 1603
Fragments arranged by size:
2144 1603 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2530
Size: 4163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1339 2218 2710 3008 1042
Size: 297 879 492 298 2197
Fragments arranged by size:
2197 879 492 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 450 3867 450
Size: 3417 746
BsaAI yAC'GTr
Cuts at: 1516 1516
Size: 4163
BsaBI GATnn'nnATC
Cuts at: 6 1677 3714 6
Size: 1671 2037 455
Fragments arranged by size:
2037 1671 455
BsaHI Gr'CG_yC
Cuts at: 1594 1842 2544 4040 1594
Size: 248 702 1496 1717
Fragments arranged by size:
1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1352 1873 2551 2825 2972 3383 1352
Size: 521 678 274 147 411 2132
Fragments arranged by size:
2132 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 270 587 1588 2346 2923 3419 270
Size: 317 1001 758 577 496 1014
Fragments arranged by size:
1014 1001 758 577 496 317
BsiEI CG_ry'CG
Cuts at: 104 318 960 1705 1751 2959 104
Size: 214 642 745 46 1208 1308
Fragments arranged by size:
1308 1208 745 642 214 46
BsiHKAI G_wGCw'C
Cuts at: 1177 1955 2145 2937 1177
Size: 778 190 792 2403
Fragments arranged by size:
2403 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 966 1177 1788 1881 1955 2145 2207 2937 966
Size: 211 611 93 74 190 62 730 2192
Fragments arranged by size:
2192 730 611 211 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3383 3383
Size: 4163
BspHI T'CATG_A
Cuts at: 908 3339 908
Size: 2431 1732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 180 347 1213 1427 1729 2110 180
Size: 167 866 214 302 381 2233
Fragments arranged by size:
2233 866 381 302 214 167
BsrBI CCG'CTC
Cuts at: 50 320 2455 2509 3993 4158 50
Size: 270 2135 54 1484 165 55
Fragments arranged by size:
2135 1484 270 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2075 2075
Size: 4163
BsrFI r'CCGG_y
Cuts at: 2161 2342 2551 2161
Size: 181 209 3773
Fragments arranged by size:
3773 209 181
BssSI C'ACGA_G
Cuts at: 2434 2792 2434
Size: 358 3805
BstAPI GCAn_nnn'nTGC
Cuts at: 1540 1540
Size: 4163
BstBI TT'CG_AA
Cuts at: 2524 2524
Size: 4163
BstXI CCAn_nnnn'nTGG
Cuts at: 134 134
Size: 4163
BstYI r'GATC_y
Cuts at: 1 123 716 1061 1188 1655 1678 2013 2259
Size: 122 593 345 127 467 23 335 246
Cuts at: 2259 3271 3357 3369 3715 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 593 467 449 346 345 335 246
127 122 86 23 12
BtgI C'CryG_G
Cuts at: 1047 1047
Size: 4163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 222 2113 2294 2457 222
Size: 1891 181 163 1928
Fragments arranged by size:
1928 1891 181 163
BtsI GCAGTG_nn'
Cuts at: 1406 1406
Size: 4163
DraI TTT'AAA
Cuts at: 1162 1162
Size: 4163
DrdI GACnn_nn'nnGTC
Cuts at: 1869 2727 1869
Size: 858 3305
EaeI y'GGCC_r
Cuts at: 315 356 518 957 1032 1748 1922 2313 2340
Size: 41 162 439 75 716 174 391 27
Cuts at: 2340 3685 315
Size: 1345 793
Fragments arranged by size:
1345 793 716 439 391 174 162 75
41 27
EagI C'GGCC_G
Cuts at: 315 957 1748 315
Size: 642 791 2730
Fragments arranged by size:
2730 791 642
EarI CTCTTCn'nnn_
Cuts at: 1152 2186 2396 1152
Size: 1034 210 2919
Fragments arranged by size:
2919 1034 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 619 717 2371 2681 2827 3973 619
Size: 98 1654 310 146 1146 809
Fragments arranged by size:
1654 1146 809 310 146 98
EcoICRI GAG'CTC
Cuts at: 1175 1175
Size: 4163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 1988 2420 3167 3435 475
Size: 1513 432 747 268 1203
Fragments arranged by size:
1513 1203 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 436 436
Size: 4163
EcoO109I rG'GnC_Cy
Cuts at: 675 914 963 1302 675
Size: 239 49 339 3536
Fragments arranged by size:
3536 339 239 49
EcoRI G'AATT_C
Cuts at: 1167 1167
Size: 4163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 576 1113 2182 576
Size: 537 1069 2557
Fragments arranged by size:
2557 1069 537
FspI TGC'GCA
Cuts at: 1944 1944
Size: 4163
HaeII r_GCGC'y
Cuts at: 945 1597 1845 2867 3846 945
Size: 652 248 1022 979 1262
Fragments arranged by size:
1262 1022 979 652 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 708 740 2005 2035 2037 2067 708
Size: 32 1265 30 2 30 2804
Fragments arranged by size:
2804 1265 32 30 30 2
HincII GTy'rAC
Cuts at: 465 1202 465
Size: 737 3426
KasI G'GCGC_C
Cuts at: 1593 1841 1593
Size: 248 3915
KpnI G_GTAC'C
Cuts at: 1183 1183
Size: 4163
MluI A'CGCG_T
Cuts at: 3596 3596
Size: 4163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1175 2834 3018 1175
Size: 1659 184 2320
Fragments arranged by size:
2320 1659 184
MscI TGG'CCA
Cuts at: 520 1924 520
Size: 1404 2759
MslI CAynn'nnrTG
Cuts at: 132 163 432 502 907 2152 2279 132
Size: 31 269 70 405 1245 127 2016
Fragments arranged by size:
2016 1245 405 269 127 70 31
MspA1I CmG'CkG
Cuts at: 750 1274 1588 1948 2961 3206 750
Size: 524 314 360 1013 245 1707
Fragments arranged by size:
1707 1013 524 360 314 245
NaeI GCC'GGC
Cuts at: 2344 2344
Size: 4163
NarI GG'CG_CC
Cuts at: 1594 1842 1594
Size: 248 3915
NcoI C'CATG_G
Cuts at: 1047 1047
Size: 4163
NgoMIV G'CCGG_C
Cuts at: 2342 2342
Size: 4163
NsiI A_TGCA'T
Cuts at: 1076 1076
Size: 4163
NspI r_CATG'y
Cuts at: 168 1216 1716 2623 168
Size: 1048 500 907 1708
Fragments arranged by size:
1708 1048 907 500
PciI A'CATG_T
Cuts at: 2619 2619
Size: 4163
PfoI T'CCnGG_A
Cuts at: 926 926
Size: 4163
PmeI GTTT'AAAC
Cuts at: 1162 1162
Size: 4163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 4131
PpuMI rG'GwC_Cy
Cuts at: 675 914 675
Size: 239 3924
PspOMI G'GGCC_C
Cuts at: 962 962
Size: 4163
PstI C_TGCA'G
Cuts at: 177 1210 1895 177
Size: 1033 685 2445
Fragments arranged by size:
2445 1033 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4163
PvuII CAG'CTG
Cuts at: 1588 1948 1588
Size: 360 3803
RsrII CG'GwC_CG
Cuts at: 2358 2358
Size: 4163
SacI G_AGCT'C
Cuts at: 1177 1177
Size: 4163
SalI G'TCGA_C
Cuts at: 1200 1200
Size: 4163
SapI GCTCTTCn'nnn_
Cuts at: 2186 2396 2186
Size: 210 3953
SbfI CC_TGCA'GG
Cuts at: 1210 1210
Size: 4163
ScaI AGT'ACT
Cuts at: 3680 3680
Size: 4163
SexAI A'CCwGG_T
Cuts at: 884 884
Size: 4163
SfcI C'TryA_G
Cuts at: 33 173 471 1206 1891 2884 3075 33
Size: 140 298 735 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 298 191 140
SfoI GGC'GCC
Cuts at: 1595 1843 1595
Size: 248 3915
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4163
SmaI CCC'GGG
Cuts at: 1185 1185
Size: 4163
SmlI C'TyrA_G
Cuts at: 1057 1318 2233 2725 2987 1057
Size: 261 915 492 262 2233
Fragments arranged by size:
2233 915 492 262 261
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4163
SphI G_CATG'C
Cuts at: 168 1216 1716 168
Size: 1048 500 2615
Fragments arranged by size:
2615 1048 500
SspI AAT'ATT
Cuts at: 117 117
Size: 4163
StyI C'CwwG_G
Cuts at: 353 401 1047 1297 1651 353
Size: 48 646 250 354 2865
Fragments arranged by size:
2865 646 354 250 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1746 1879 2338 2547 1746
Size: 133 459 209 3362
Fragments arranged by size:
3362 459 209 133
TatI w'GTAC_w
Cuts at: 3678 3678
Size: 4163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3680 3712 3680
Size: 32 4131
Tth111I GACn'n_nGTC
Cuts at: 1960 1960
Size: 4163
XbaI T'CTAG_A
Cuts at: 1194 1194
Size: 4163
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 981 1658 981
Size: 677 3486
XmaI C'CCGG_G
Cuts at: 1183 1183
Size: 4163
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BglI BmtI BpmI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI