Restriction map for
pF1KB9841
(Circular) (Six-base) MAPSORT of: pF1KB9841.seq Check: 2479 from: 1 to: 4187
pF1KB9841 4187 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 1137 1137
Size: 4187
AccI GT'mk_AC
Cuts at: 1225 1225
Size: 4187
Acc65I G'GTAC_C
Cuts at: 1203 1203
Size: 4187
AclI AA'CG_TT
Cuts at: 4027 4027
Size: 4187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 939 2012 2444 3191 3459 939
Size: 1073 432 747 268 1667
Fragments arranged by size:
1667 1073 747 432 268
AflIII A'CryG_T
Cuts at: 1539 2643 3620 1539
Size: 1104 977 2106
Fragments arranged by size:
2106 1104 977
AgeI A'CCGG_T
Cuts at: 2575 2575
Size: 4187
AleI CACnn'nnGTG
Cuts at: 565 565
Size: 4187
AlwNI CAG_nnn'CTG
Cuts at: 229 918 1492 3059 229
Size: 689 574 1567 1357
Fragments arranged by size:
1567 1357 689 574
ApaLI G'TGCA_C
Cuts at: 2957 2957
Size: 4187
ApoI r'AATT_y
Cuts at: 132 940 1191 3540 3796 132
Size: 808 251 2349 256 523
Fragments arranged by size:
2349 808 523 256 251
AseI AT'TA_AT
Cuts at: 20 20
Size: 4187
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
AvaI C'yCGr_G
Cuts at: 1207 1207
Size: 4187
BamHI G'GATC_C
Cuts at: 1212 1212
Size: 4187
BanI G'GyrC_C
Cuts at: 1074 1203 1617 1865 1900 1074
Size: 129 414 248 35 3361
Fragments arranged by size:
3361 414 248 129 35
BanII G_rGCy'C
Cuts at: 466 1201 2231 466
Size: 735 1030 2422
Fragments arranged by size:
2422 1030 735
BbeI G_GCGC'C
Cuts at: 1621 1869 1621
Size: 248 3939
BbsI GAAGACnn'nnnn_
Cuts at: 227 717 227
Size: 490 3697
BciVI GTATCCnnnnn_n'
Cuts at: 711 2085 2846 4187 711
Size: 1374 761 1341 711
Fragments arranged by size:
1374 1341 761 711
BclI T'GATC_A
Cuts at: 1707 1707
Size: 4187
BglI GCCn_nnn'nGGC
Cuts at: 445 445
Size: 4187
BglII A'GATC_T
Cuts at: 1702 3739 1702
Size: 2037 2150
BlpI GC'TnA_GC
Cuts at: 1299 1299
Size: 4187
Bme1580I G_kGCm'C
Cuts at: 223 550 1079 1812 1905 2961 223
Size: 327 529 733 93 1056 1449
Fragments arranged by size:
1449 1056 733 529 327 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 1399 1480 1815 3959 348
Size: 1051 81 335 2144 576
Fragments arranged by size:
2144 1051 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2554 2554
Size: 4187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 526 940 160
Size: 366 414 3407
Fragments arranged by size:
3407 414 366
Bpu10I CC'TnA_GC
Cuts at: 769 769
Size: 4187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 2242 2734 3032 1363
Size: 879 492 298 2518
Fragments arranged by size:
2518 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3891 3891
Size: 4187
BsaAI yAC'GTr
Cuts at: 699 1540 699
Size: 841 3346
BsaBI GATnn'nnATC
Cuts at: 6 1701 3738 6
Size: 1695 2037 455
Fragments arranged by size:
2037 1695 455
BsaHI Gr'CG_yC
Cuts at: 1134 1618 1866 2568 4064 1134
Size: 484 248 702 1496 1257
Fragments arranged by size:
1496 1257 702 484 248
BsaWI w'CCGG_w
Cuts at: 263 1376 1897 2575 2849 2996 3407 263
Size: 1113 521 678 274 147 411 1043
Fragments arranged by size:
1113 1043 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 660 690 746 776 660
Size: 30 56 30 4071
Fragments arranged by size:
4071 56 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 754 754
Size: 4187
BseYI C'CCAG_C
Cuts at: 216 512 1612 2370 2947 3443 216
Size: 296 1100 758 577 496 960
Fragments arranged by size:
1100 960 758 577 496 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 591 737 873 591
Size: 146 136 3905
Fragments arranged by size:
3905 146 136
BsiEI CG_ry'CG
Cuts at: 104 164 1729 1775 2983 104
Size: 60 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 60 46
BsiHKAI G_wGCw'C
Cuts at: 1201 1979 2169 2961 1201
Size: 778 190 792 2427
Fragments arranged by size:
2427 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 366 366
Size: 4187
Bsp1286I G_dGCh'C
Cuts at: 223 466 550 1079 1201 1812 1905 1979 2169
Size: 243 84 529 122 611 93 74 190
Cuts at: 2169 2231 2961 223
Size: 62 730 1449
Fragments arranged by size:
1449 730 611 529 243 190 122 93
84 74 62
BspEI T'CCGG_A
Cuts at: 3407 3407
Size: 4187
BspHI T'CATG_A
Cuts at: 3363 3363
Size: 4187
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1237 1451 1753 2134 1237
Size: 214 302 381 3290
Fragments arranged by size:
3290 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2479 2533 4017 4182 50
Size: 2429 54 1484 165 55
Fragments arranged by size:
2429 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2099 2099
Size: 4187
BsrFI r'CCGG_y
Cuts at: 2185 2366 2575 2185
Size: 181 209 3797
Fragments arranged by size:
3797 209 181
BsrGI T'GTAC_A
Cuts at: 380 380
Size: 4187
BssSI C'ACGA_G
Cuts at: 211 2458 2816 211
Size: 2247 358 1582
Fragments arranged by size:
2247 1582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 405 1564 405
Size: 1159 3028
BstBI TT'CG_AA
Cuts at: 2548 2548
Size: 4187
BstEII G'GTnAC_C
Cuts at: 996 1096 996
Size: 100 4087
BstXI CCAn_nnnn'nTGG
Cuts at: 600 859 600
Size: 259 3928
BstYI r'GATC_y
Cuts at: 1 1212 1679 1702 2037 2283 3295 3381 3393
Size: 1211 467 23 335 246 1012 86 12
Cuts at: 3393 3739 1
Size: 346 449
Fragments arranged by size:
1211 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1070 1070
Size: 4187
BtgI C'CryG_G
Cuts at: 107 164 593 829 1110 107
Size: 57 429 236 281 3184
Fragments arranged by size:
3184 429 281 236 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2137 2318 2481 2137
Size: 181 163 3843
Fragments arranged by size:
3843 181 163
BtsI GCAGTG_nn'
Cuts at: 1430 1430
Size: 4187
DraI TTT'AAA
Cuts at: 1186 1186
Size: 4187
DraIII CAC_nnn'GTG
Cuts at: 405 405
Size: 4187
DrdI GACnn_nn'nnGTC
Cuts at: 994 1893 2751 994
Size: 899 858 2430
Fragments arranged by size:
2430 899 858
EaeI y'GGCC_r
Cuts at: 1059 1772 1946 2337 2364 3709 1059
Size: 713 174 391 27 1345 1537
Fragments arranged by size:
1537 1345 713 391 174 27
EagI C'GGCC_G
Cuts at: 1772 1772
Size: 4187
EarI CTCTTCn'nnn_
Cuts at: 199 2210 2420 199
Size: 2011 210 1966
Fragments arranged by size:
2011 1966 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2395 2705 2851 3997 2395
Size: 310 146 1146 2585
Fragments arranged by size:
2585 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1199 1199
Size: 4187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 526 939 940 2012 2444 3191 3459 160
Size: 366 413 1 1072 432 747 268 888
Fragments arranged by size:
1072 888 747 432 413 366 268 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1034 1034
Size: 4187
EcoO109I rG'GnC_Cy
Cuts at: 1326 1326
Size: 4187
EcoRI G'AATT_C
Cuts at: 1191 1191
Size: 4187
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 741 2206 741
Size: 1465 2722
FspI TGC'GCA
Cuts at: 409 1968 409
Size: 1559 2628
FspAI rTGC'GCAy
Cuts at: 409 409
Size: 4187
HaeII r_GCGC'y
Cuts at: 1621 1869 2891 3870 1621
Size: 248 1022 979 1938
Fragments arranged by size:
1938 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 660 692 1159 1191 2029 2059 2061 2091 660
Size: 32 467 32 838 30 2 30 2756
Fragments arranged by size:
2756 838 467 32 32 30 30 2
HincII GTy'rAC
Cuts at: 690 1226 690
Size: 536 3651
KasI G'GCGC_C
Cuts at: 1617 1865 1617
Size: 248 3939
KpnI G_GTAC'C
Cuts at: 1207 1207
Size: 4187
MluI A'CGCG_T
Cuts at: 3620 3620
Size: 4187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3042 2858
Size: 184 4003
MscI TGG'CCA
Cuts at: 1061 1948 1061
Size: 887 3300
MslI CAynn'nnrTG
Cuts at: 565 592 598 2176 2303 565
Size: 27 6 1578 127 2449
Fragments arranged by size:
2449 1578 127 27 6
MspA1I CmG'CkG
Cuts at: 226 831 1298 1612 1972 2985 3230 226
Size: 605 467 314 360 1013 245 1183
Fragments arranged by size:
1183 1013 605 467 360 314 245
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 4187
NarI GG'CG_CC
Cuts at: 1618 1866 1618
Size: 248 3939
NcoI C'CATG_G
Cuts at: 107 593 1110 107
Size: 486 517 3184
Fragments arranged by size:
3184 517 486
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 4187
NspI r_CATG'y
Cuts at: 414 658 1240 1740 2647 414
Size: 244 582 500 907 1954
Fragments arranged by size:
1954 907 582 500 244
PasI CC'CwG_GG
Cuts at: 931 931
Size: 4187
PciI A'CATG_T
Cuts at: 2643 2643
Size: 4187
PfoI T'CCnGG_A
Cuts at: 137 137
Size: 4187
PmeI GTTT'AAAC
Cuts at: 1186 1186
Size: 4187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 4155
PstI C_TGCA'G
Cuts at: 350 1234 1919 350
Size: 884 685 2618
Fragments arranged by size:
2618 884 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4187
PvuII CAG'CTG
Cuts at: 226 1612 1972 226
Size: 1386 360 2441
Fragments arranged by size:
2441 1386 360
RsrII CG'GwC_CG
Cuts at: 2382 2382
Size: 4187
SacI G_AGCT'C
Cuts at: 1201 1201
Size: 4187
SacII CC_GC'GG
Cuts at: 832 832
Size: 4187
SalI G'TCGA_C
Cuts at: 1224 1224
Size: 4187
SapI GCTCTTCn'nnn_
Cuts at: 2210 2420 2210
Size: 210 3977
SbfI CC_TGCA'GG
Cuts at: 1234 1234
Size: 4187
ScaI AGT'ACT
Cuts at: 622 3704 622
Size: 3082 1105
SexAI A'CCwGG_T
Cuts at: 992 1092 992
Size: 100 4087
SfcI C'TryA_G
Cuts at: 33 346 1230 1915 2908 3099 33
Size: 313 884 685 993 191 1121
Fragments arranged by size:
1121 993 884 685 313 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 445 445
Size: 4187
SfoI GGC'GCC
Cuts at: 1619 1867 1619
Size: 248 3939
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4187
SmaI CCC'GGG
Cuts at: 1209 1209
Size: 4187
SmlI C'TyrA_G
Cuts at: 1342 2257 2749 3011 1342
Size: 915 492 262 2518
Fragments arranged by size:
2518 915 492 262
SnaBI TAC'GTA
Cuts at: 699 699
Size: 4187
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4187
SphI G_CATG'C
Cuts at: 414 1240 1740 414
Size: 826 500 2861
Fragments arranged by size:
2861 826 500
StyI C'CwwG_G
Cuts at: 107 593 1110 1321 1675 107
Size: 486 517 211 354 2619
Fragments arranged by size:
2619 517 486 354 211
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 330 1770 1903 2362 2571 330
Size: 1440 133 459 209 1946
Fragments arranged by size:
1946 1440 459 209 133
TatI w'GTAC_w
Cuts at: 147 380 620 3702 147
Size: 233 240 3082 632
Fragments arranged by size:
3082 632 240 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 746 778 3704 3736 746
Size: 32 2926 32 1197
Fragments arranged by size:
2926 1197 32 32
Tth111I GACn'n_nGTC
Cuts at: 1984 1984
Size: 4187
XbaI T'CTAG_A
Cuts at: 1218 1218
Size: 4187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 372 597 1054 1682 372
Size: 225 457 628 2877
Fragments arranged by size:
2877 628 457 225
XmaI C'CCGG_G
Cuts at: 1207 1207
Size: 4187
XmnI GAAnn'nnTTC
Cuts at: 724 724
Size: 4187
ZraI GAC'GTC
Cuts at: 1135 1135
Size: 4187
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BssHII BstZ17I ClaI CspCI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SgrAI SrfI SspI StuI SwaI XhoI