Restriction map for
pF1KB9842
(Circular) (Six-base) MAPSORT of: pF1KB9842.seq Check: 2494 from: 1 to: 4193
pF1KB9842 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 362 668 1231 362
Size: 306 563 3324
Fragments arranged by size:
3324 563 306
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1148 2018 2450 3197 3465 502
Size: 646 870 432 747 268 1230
Fragments arranged by size:
1230 870 747 646 432 268
AfeI AGC'GCT
Cuts at: 583 583
Size: 4193
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 614 1498 3065 614
Size: 884 1567 1742
Fragments arranged by size:
1742 1567 884
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 595 1197 3546 3802 595
Size: 602 2349 256 986
Fragments arranged by size:
2349 986 602 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1213 1213
Size: 4193
BamHI G'GATC_C
Cuts at: 743 1218 743
Size: 475 3718
BanI G'GyrC_C
Cuts at: 1209 1623 1871 1906 1209
Size: 414 248 35 3496
Fragments arranged by size:
3496 414 248 35
BanII G_rGCy'C
Cuts at: 993 1207 2237 993
Size: 214 1030 2949
Fragments arranged by size:
2949 1030 214
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 963 997 963
Size: 34 4159
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 782 1109 1713 782
Size: 327 604 3262
Fragments arranged by size:
3262 604 327
BglII A'GATC_T
Cuts at: 162 1708 3745 162
Size: 1546 2037 610
Fragments arranged by size:
2037 1546 610
BlpI GC'TnA_GC
Cuts at: 289 1305 289
Size: 1016 3177
Bme1580I G_kGCm'C
Cuts at: 1172 1818 1911 2967 1172
Size: 646 93 1056 2398
Fragments arranged by size:
2398 1056 646 93
BmrI ACTGGGnnnn_n'
Cuts at: 747 1405 1486 1821 3965 747
Size: 658 81 335 2144 975
Fragments arranged by size:
2144 975 658 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1369 2248 2740 3038 1069
Size: 300 879 492 298 2224
Fragments arranged by size:
2224 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 477 952 3897 477
Size: 475 2945 773
Fragments arranged by size:
2945 773 475
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 1707 3744 6
Size: 1701 2037 455
Fragments arranged by size:
2037 1701 455
BsaHI Gr'CG_yC
Cuts at: 1624 1872 2574 4070 1624
Size: 248 702 1496 1747
Fragments arranged by size:
1747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1072 1072
Size: 4193
BseYI C'CCAG_C
Cuts at: 297 687 1618 2376 2953 3449 297
Size: 390 931 758 577 496 1041
Fragments arranged by size:
1041 931 758 577 496 390
BsiEI CG_ry'CG
Cuts at: 104 345 987 1735 1781 2989 104
Size: 241 642 748 46 1208 1308
Fragments arranged by size:
1308 1208 748 642 241 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
BsiWI C'GTAC_G
Cuts at: 814 814
Size: 4193
Bsp1286I G_dGCh'C
Cuts at: 993 1172 1207 1818 1911 1985 2175 2237 2967
Size: 179 35 611 93 74 190 62 730
Cuts at: 2967 993
Size: 2219
Fragments arranged by size:
2219 730 611 190 179 93 74 62
35
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 935 3369 935
Size: 2434 1759
BspMI ACCTGCnnnn'nnnn_
Cuts at: 374 1243 1457 1759 2140 374
Size: 869 214 302 381 2427
Fragments arranged by size:
2427 869 381 302 214
BsrBI CCG'CTC
Cuts at: 50 347 2485 2539 4023 4188 50
Size: 297 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 297 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2105 2105
Size: 4193
BsrFI r'CCGG_y
Cuts at: 2191 2372 2581 2191
Size: 181 209 3803
Fragments arranged by size:
3803 209 181
BssSI C'ACGA_G
Cuts at: 310 2464 2822 310
Size: 2154 358 1681
Fragments arranged by size:
2154 1681 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 760 760
Size: 4193
BstYI r'GATC_y
Cuts at: 1 162 743 1218 1685 1708 2043 2289 3301
Size: 161 581 475 467 23 335 246 1012
Cuts at: 3301 3387 3399 3745 1
Size: 86 12 346 449
Fragments arranged by size:
1012 581 475 467 449 346 335 246
161 86 23 12
BtgI C'CryG_G
Cuts at: 107 1074 107
Size: 967 3226
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2143 2324 2487 2143
Size: 181 163 3849
Fragments arranged by size:
3849 181 163
BtsI GCAGTG_nn'
Cuts at: 775 1436 775
Size: 661 3532
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 1899 2757 1899
Size: 858 3335
EaeI y'GGCC_r
Cuts at: 342 383 545 1059 1778 1952 2343 2370 3715
Size: 41 162 514 719 174 391 27 1345
Cuts at: 3715 342
Size: 820
Fragments arranged by size:
1345 820 719 514 391 174 162 41
27
EagI C'GGCC_G
Cuts at: 342 1778 342
Size: 1436 2757
EarI CTCTTCn'nnn_
Cuts at: 1164 2216 2426 1164
Size: 1052 210 2931
Fragments arranged by size:
2931 1052 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 744 2401 2711 2857 4003 744
Size: 1657 310 146 1146 934
Fragments arranged by size:
1657 1146 934 310 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1148 2018 2450 3197 3465 502
Size: 646 870 432 747 268 1230
Fragments arranged by size:
1230 870 747 646 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 4193
EcoO109I rG'GnC_Cy
Cuts at: 941 1332 941
Size: 391 3802
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 603 2212 603
Size: 1609 2584
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 585 972 1627 1875 2897 3876 585
Size: 387 655 248 1022 979 902
Fragments arranged by size:
1022 979 902 655 387 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2035 2065 2067 2097 2035
Size: 30 2 30 4131
Fragments arranged by size:
4131 30 30 2
HincII GTy'rAC
Cuts at: 492 1232 492
Size: 740 3453
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2864 3048 2864
Size: 184 4009
MscI TGG'CCA
Cuts at: 547 1061 1954 547
Size: 514 893 2786
Fragments arranged by size:
2786 893 514
MslI CAynn'nnrTG
Cuts at: 459 529 934 2182 2309 459
Size: 70 405 1248 127 2343
Fragments arranged by size:
2343 1248 405 127 70
MspA1I CmG'CkG
Cuts at: 1028 1304 1618 1978 2991 3236 1028
Size: 276 314 360 1013 245 1985
Fragments arranged by size:
1985 1013 360 314 276 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 107 1074 107
Size: 967 3226
NdeI CA'TA_TG
Cuts at: 601 601
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 2653 1246
Size: 500 907 2786
Fragments arranged by size:
2786 907 500
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PfoI T'CCnGG_A
Cuts at: 953 953
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 941 941
Size: 4193
PstI C_TGCA'G
Cuts at: 1240 1925 1240
Size: 685 3508
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 1028 1618 1978 1028
Size: 590 360 3243
Fragments arranged by size:
3243 590 360
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 1164 2216 2426 1164
Size: 1052 210 2931
Fragments arranged by size:
2931 1052 210
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SexAI A'CCwGG_T
Cuts at: 911 911
Size: 4193
SfcI C'TryA_G
Cuts at: 33 498 1236 1921 2914 3105 33
Size: 465 738 685 993 191 1121
Fragments arranged by size:
1121 993 738 685 465 191
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 1084 1348 2263 2755 3017 1084
Size: 264 915 492 262 2260
Fragments arranged by size:
2260 915 492 264 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StuI AGG'CCT
Cuts at: 1143 1143
Size: 4193
StyI C'CwwG_G
Cuts at: 107 380 428 1074 1327 1681 107
Size: 273 48 646 253 354 2619
Fragments arranged by size:
2619 646 354 273 253 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 3708 3708
Size: 4193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 1224 1224
Size: 4193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1008 1688 1008
Size: 680 3513
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BsgI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI