Restriction map for
pF1KB9845
(Circular) (Six-base) MAPSORT of: pF1KB9845.seq Check: 4651 from: 1 to: 4232
pF1KB9845 4232 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 278 1270 278
Size: 992 3240
Acc65I G'GTAC_C
Cuts at: 679 1248 679
Size: 569 3663
AclI AA'CG_TT
Cuts at: 4072 4072
Size: 4232
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2489 3236 3504 2057
Size: 432 747 268 2785
Fragments arranged by size:
2785 747 432 268
AflIII A'CryG_T
Cuts at: 374 1584 2688 3665 374
Size: 1210 1104 977 941
Fragments arranged by size:
1210 1104 977 941
AgeI A'CCGG_T
Cuts at: 551 2620 551
Size: 2069 2163
AhdI GACnn_n'nnGTC
Cuts at: 1037 1037
Size: 4232
AleI CACnn'nnGTG
Cuts at: 772 772
Size: 4232
AlwNI CAG_nnn'CTG
Cuts at: 1537 3104 1537
Size: 1567 2665
ApaI G_GGCC'C
Cuts at: 966 966
Size: 4232
ApaLI G'TGCA_C
Cuts at: 765 3002 765
Size: 2237 1995
ApoI r'AATT_y
Cuts at: 1152 1236 3585 3841 1152
Size: 84 2349 256 1543
Fragments arranged by size:
2349 1543 256 84
AseI AT'TA_AT
Cuts at: 20 823 20
Size: 803 3429
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
AvaI C'yCGr_G
Cuts at: 1115 1252 1115
Size: 137 4095
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 546 579 663 671 696 704 546
Size: 33 84 8 25 8 4074
Fragments arranged by size:
4074 84 33 25 8 8
BamHI G'GATC_C
Cuts at: 1257 1257
Size: 4232
BanI G'GyrC_C
Cuts at: 679 1248 1662 1910 1945 679
Size: 569 414 248 35 2966
Fragments arranged by size:
2966 569 414 248 35
BanII G_rGCy'C
Cuts at: 966 1246 2276 966
Size: 280 1030 2922
Fragments arranged by size:
2922 1030 280
BbeI G_GCGC'C
Cuts at: 1666 1914 1666
Size: 248 3984
BbsI GAAGACnn'nnnn_
Cuts at: 330 1178 330
Size: 848 3384
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2891 4232 2130
Size: 761 1341 2130
Fragments arranged by size:
2130 1341 761
BclI T'GATC_A
Cuts at: 1752 1752
Size: 4232
BfrBI ATG'CAT
Cuts at: 539 539
Size: 4232
BglI GCCn_nnn'nGGC
Cuts at: 160 160
Size: 4232
BglII A'GATC_T
Cuts at: 849 1747 3784 849
Size: 898 2037 1297
Fragments arranged by size:
2037 1297 898
BlpI GC'TnA_GC
Cuts at: 1344 1344
Size: 4232
Bme1580I G_kGCm'C
Cuts at: 549 769 966 1857 1950 3006 549
Size: 220 197 891 93 1056 1775
Fragments arranged by size:
1775 1056 891 220 197 93
BmrI ACTGGGnnnn_n'
Cuts at: 1444 1525 1860 4004 1444
Size: 81 335 2144 1672
Fragments arranged by size:
2144 1672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 4232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 375
Size: 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 978 1408 2287 2779 3077 562
Size: 416 430 879 492 298 1717
Fragments arranged by size:
1717 879 492 430 416 298
BsaI GGTCTCn'nnnn_
Cuts at: 3936 3936
Size: 4232
BsaAI yAC'GTr
Cuts at: 408 1022 1585 408
Size: 614 563 3055
Fragments arranged by size:
3055 614 563
BsaBI GATnn'nnATC
Cuts at: 6 616 1746 3783 6
Size: 610 1130 2037 455
Fragments arranged by size:
2037 1130 610 455
BsaHI Gr'CG_yC
Cuts at: 1663 1911 2613 4109 1663
Size: 248 702 1496 1786
Fragments arranged by size:
1786 1496 702 248
BsaWI w'CCGG_w
Cuts at: 551 1421 1942 2620 2894 3041 3452 551
Size: 870 521 678 274 147 411 1331
Fragments arranged by size:
1331 870 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 854 885 207
Size: 647 31 3554
Fragments arranged by size:
3554 647 31
BseYI C'CCAG_C
Cuts at: 984 1657 2415 2992 3488 984
Size: 673 758 577 496 1728
Fragments arranged by size:
1728 758 673 577 496
BsiEI CG_ry'CG
Cuts at: 104 197 773 1774 1820 3028 104
Size: 93 576 1001 46 1208 1308
Fragments arranged by size:
1308 1208 1001 576 93 46
BsiHKAI G_wGCw'C
Cuts at: 769 1246 2024 2214 3006 769
Size: 477 778 190 792 1995
Fragments arranged by size:
1995 792 778 477 190
BsmI GAATG_Cn'
Cuts at: 612 612
Size: 4232
BsmBI CGTCTCn'nnnn_
Cuts at: 219 219
Size: 4232
Bsp1286I G_dGCh'C
Cuts at: 549 769 966 1246 1857 1950 2024 2214 2276
Size: 220 197 280 611 93 74 190 62
Cuts at: 2276 3006 549
Size: 730 1775
Fragments arranged by size:
1775 730 611 280 220 197 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3452 3452
Size: 4232
BspHI T'CATG_A
Cuts at: 3408 3408
Size: 4232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 746 1282 1496 1798 2179 275
Size: 471 536 214 302 381 2328
Fragments arranged by size:
2328 536 471 381 302 214
BsrBI CCG'CTC
Cuts at: 50 359 2524 2578 4062 4227 50
Size: 309 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 309 165 55 54
BsrDI GCAATG_nn'
Cuts at: 995 2144 995
Size: 1149 3083
BsrFI r'CCGG_y
Cuts at: 126 163 270 551 590 2230 2411 2620 126
Size: 37 107 281 39 1640 181 209 1738
Fragments arranged by size:
1738 1640 281 209 181 107 39 37
BsrGI T'GTAC_A
Cuts at: 371 371
Size: 4232
BssSI C'ACGA_G
Cuts at: 2503 2861 2503
Size: 358 3874
BstAPI GCAn_nnn'nTGC
Cuts at: 994 1609 994
Size: 615 3617
BstBI TT'CG_AA
Cuts at: 2593 2593
Size: 4232
BstYI r'GATC_y
Cuts at: 1 458 849 1257 1724 1747 2082 2328 3340
Size: 457 391 408 467 23 335 246 1012
Cuts at: 3340 3426 3438 3784 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 457 449 408 391 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 773 773
Size: 4232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2182 2363 2526 2182
Size: 181 163 3888
Fragments arranged by size:
3888 181 163
BtsI GCAGTG_nn'
Cuts at: 203 1475 203
Size: 1272 2960
ClaI AT'CG_AT
Cuts at: 880 880
Size: 4232
DraI TTT'AAA
Cuts at: 1231 1231
Size: 4232
DrdI GACnn_nn'nnGTC
Cuts at: 1938 2796 1938
Size: 858 3374
EaeI y'GGCC_r
Cuts at: 124 161 320 392 776 1817 1991 2382 2409
Size: 37 159 72 384 1041 174 391 27
Cuts at: 2409 3754 124
Size: 1345 602
Fragments arranged by size:
1345 1041 602 391 384 174 159 72
37 27
EagI C'GGCC_G
Cuts at: 1817 1817
Size: 4232
EarI CTCTTCn'nnn_
Cuts at: 673 2255 2465 673
Size: 1582 210 2440
Fragments arranged by size:
2440 1582 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2440 2750 2896 4042 2440
Size: 310 146 1146 2630
Fragments arranged by size:
2630 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1244 1244
Size: 4232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 2057 2489 3236 3504 375
Size: 1682 432 747 268 1103
Fragments arranged by size:
1682 1103 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 962 1371 962
Size: 409 3823
EcoRI G'AATT_C
Cuts at: 1236 1236
Size: 4232
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 267 1148 2251 267
Size: 881 1103 2248
Fragments arranged by size:
2248 1103 881
FspI TGC'GCA
Cuts at: 2013 2013
Size: 4232
HaeII r_GCGC'y
Cuts at: 1666 1914 2936 3915 1666
Size: 248 1022 979 1983
Fragments arranged by size:
1983 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 709 741 1023 1055 2074 2104 2106 2136 709
Size: 32 282 32 1019 30 2 30 2805
Fragments arranged by size:
2805 1019 282 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1271 1271
Size: 4232
HindIII A'AGCT_T
Cuts at: 1130 1130
Size: 4232
KasI G'GCGC_C
Cuts at: 1662 1910 1662
Size: 248 3984
KpnI G_GTAC'C
Cuts at: 683 1252 683
Size: 569 3663
MluI A'CGCG_T
Cuts at: 3665 3665
Size: 4232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3087 2903
Size: 184 4048
MscI TGG'CCA
Cuts at: 322 778 1993 322
Size: 456 1215 2561
Fragments arranged by size:
2561 1215 456
MslI CAynn'nnrTG
Cuts at: 328 367 643 772 2221 2348 328
Size: 39 276 129 1449 127 2212
Fragments arranged by size:
2212 1449 276 129 127 39
MspA1I CmG'CkG
Cuts at: 560 1343 1657 2017 3030 3275 560
Size: 783 314 360 1013 245 1517
Fragments arranged by size:
1517 1013 783 360 314 245
NaeI GCC'GGC
Cuts at: 128 272 592 2413 128
Size: 144 320 1821 1947
Fragments arranged by size:
1947 1821 320 144
NarI GG'CG_CC
Cuts at: 1663 1911 1663
Size: 248 3984
NgoMIV G'CCGG_C
Cuts at: 126 270 590 2411 126
Size: 144 320 1821 1947
Fragments arranged by size:
1947 1821 320 144
NsiI A_TGCA'T
Cuts at: 541 541
Size: 4232
NspI r_CATG'y
Cuts at: 378 539 765 1285 1785 2692 378
Size: 161 226 520 500 907 1918
Fragments arranged by size:
1918 907 520 500 226 161
PasI CC'CwG_GG
Cuts at: 585 585
Size: 4232
PciI A'CATG_T
Cuts at: 374 2688 374
Size: 2314 1918
PmeI GTTT'AAAC
Cuts at: 1231 1231
Size: 4232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3371 3403 3371
Size: 32 4200
PspOMI G'GGCC_C
Cuts at: 962 962
Size: 4232
PstI C_TGCA'G
Cuts at: 488 1279 1964 488
Size: 791 685 2756
Fragments arranged by size:
2756 791 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4232
PvuII CAG'CTG
Cuts at: 1657 2017 1657
Size: 360 3872
RsrII CG'GwC_CG
Cuts at: 166 2427 166
Size: 2261 1971
SacI G_AGCT'C
Cuts at: 1246 1246
Size: 4232
SalI G'TCGA_C
Cuts at: 1269 1269
Size: 4232
SapI GCTCTTCn'nnn_
Cuts at: 2255 2465 2255
Size: 210 4022
SbfI CC_TGCA'GG
Cuts at: 1279 1279
Size: 4232
ScaI AGT'ACT
Cuts at: 3749 3749
Size: 4232
SexAI A'CCwGG_T
Cuts at: 887 887
Size: 4232
SfcI C'TryA_G
Cuts at: 33 484 756 1275 1960 2953 3144 33
Size: 451 272 519 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 451 272 191
SfoI GGC'GCC
Cuts at: 1664 1912 1664
Size: 248 3984
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4232
SmaI CCC'GGG
Cuts at: 1254 1254
Size: 4232
SmlI C'TyrA_G
Cuts at: 577 957 1387 2302 2794 3056 577
Size: 380 430 915 492 262 1753
Fragments arranged by size:
1753 915 492 430 380 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4232
SphI G_CATG'C
Cuts at: 1285 1785 1285
Size: 500 3732
SspI AAT'ATT
Cuts at: 1188 1188
Size: 4232
StyI C'CwwG_G
Cuts at: 794 1366 1720 794
Size: 572 354 3306
Fragments arranged by size:
3306 572 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 127 1815 1948 2407 2616 127
Size: 1688 133 459 209 1743
Fragments arranged by size:
1743 1688 459 209 133
TatI w'GTAC_w
Cuts at: 371 900 3747 371
Size: 529 2847 856
Fragments arranged by size:
2847 856 529
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 460 492 812 844 3749 3781 460
Size: 32 320 32 2905 32 911
Fragments arranged by size:
2905 911 320 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2029 2029
Size: 4232
XbaI T'CTAG_A
Cuts at: 1135 1263 1135
Size: 128 4104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1727 1727
Size: 4232
XmaI C'CCGG_G
Cuts at: 1252 1252
Size: 4232
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI AscI AvrII
BbvCI BcgI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI
BssHII BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI