Restriction map for
pF1KB9846
(Circular) (Six-base) MAPSORT of: pF1KB9846.seq Check: 4960 from: 1 to: 4235
pF1KB9846 4235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 380 380
Size: 4235
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 1251 1251
Size: 4235
AclI AA'CG_TT
Cuts at: 957 4075 957
Size: 3118 1117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 958 2060 2492 3239 3507 733
Size: 225 1102 432 747 268 1461
Fragments arranged by size:
1461 1102 747 432 268 225
AfeI AGC'GCT
Cuts at: 691 914 691
Size: 223 4012
AflIII A'CryG_T
Cuts at: 1587 2691 3668 1587
Size: 1104 977 2154
Fragments arranged by size:
2154 1104 977
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 767 799 767
Size: 32 4203
AlwNI CAG_nnn'CTG
Cuts at: 129 182 1540 3107 129
Size: 53 1358 1567 1257
Fragments arranged by size:
1567 1358 1257 53
ApaI G_GGCC'C
Cuts at: 414 414
Size: 4235
ApaLI G'TGCA_C
Cuts at: 535 3005 535
Size: 2470 1765
ApoI r'AATT_y
Cuts at: 837 1239 3588 3844 837
Size: 402 2349 256 1228
Fragments arranged by size:
2349 1228 402 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 415 462 1255 415
Size: 47 793 3395
Fragments arranged by size:
3395 793 47
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 1251 1665 1913 1948 1251
Size: 414 248 35 3538
Fragments arranged by size:
3538 414 248 35
BanII G_rGCy'C
Cuts at: 414 1249 2279 414
Size: 835 1030 2370
Fragments arranged by size:
2370 1030 835
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BbsI GAAGACnn'nnnn_
Cuts at: 604 604
Size: 4235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 115 149 115
Size: 34 4201
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 414 539 1016 1860 1953 3009 414
Size: 125 477 844 93 1056 1640
Fragments arranged by size:
1640 1056 844 477 125 93
BmrI ACTGGGnnnn_n'
Cuts at: 957 1447 1528 1863 4007 957
Size: 490 81 335 2144 1185
Fragments arranged by size:
2144 1185 490 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 421 562 640 818 1114 301
Size: 120 141 78 178 296 3422
Fragments arranged by size:
3422 296 178 141 120 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 1411 2290 2782 3080 144
Size: 1267 879 492 298 1299
Fragments arranged by size:
1299 1267 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 162 1090 1210 3939 162
Size: 928 120 2729 458
Fragments arranged by size:
2729 928 458 120
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 377 467 476 1666 1914 2616 4112 377
Size: 90 9 1190 248 702 1496 500
Fragments arranged by size:
1496 1190 702 500 248 90 9
BsaWI w'CCGG_w
Cuts at: 1424 1945 2623 2897 3044 3455 1424
Size: 521 678 274 147 411 2204
Fragments arranged by size:
2204 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 1036 499
Size: 537 3698
BseYI C'CCAG_C
Cuts at: 873 909 1660 2418 2995 3491 873
Size: 36 751 758 577 496 1617
Fragments arranged by size:
1617 758 751 577 496 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 463 277
Size: 186 4049
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3031 104
Size: 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 539 1249 2027 2217 3009 539
Size: 710 778 190 792 1765
Fragments arranged by size:
1765 792 778 710 190
Bsp1286I G_dGCh'C
Cuts at: 414 539 1016 1249 1860 1953 2027 2217 2279
Size: 125 477 233 611 93 74 190 62
Cuts at: 2279 3009 414
Size: 730 1640
Fragments arranged by size:
1640 730 611 477 233 190 125 93
74 62
BspEI T'CCGG_A
Cuts at: 3455 3455
Size: 4235
BspHI T'CATG_A
Cuts at: 3411 3411
Size: 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 1285 1499 1801 2182 236
Size: 1049 214 302 381 2289
Fragments arranged by size:
2289 1049 381 302 214
BsrBI CCG'CTC
Cuts at: 50 285 2527 2581 4065 4230 50
Size: 235 2242 54 1484 165 55
Fragments arranged by size:
2242 1484 235 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4235
BsrFI r'CCGG_y
Cuts at: 521 2233 2414 2623 521
Size: 1712 181 209 2133
Fragments arranged by size:
2133 1712 209 181
BssHII G'CGCG_C
Cuts at: 220 220
Size: 4235
BssSI C'ACGA_G
Cuts at: 2506 2864 2506
Size: 358 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 543 1612 543
Size: 1069 3166
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstYI r'GATC_y
Cuts at: 1 1260 1727 1750 2085 2331 3343 3429 3441
Size: 1259 467 23 335 246 1012 86 12
Cuts at: 3441 3787 1
Size: 346 449
Fragments arranged by size:
1259 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 220 1478 220
Size: 1258 2977
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DraIII CAC_nnn'GTG
Cuts at: 441 441
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 1820 1994 2385 2412 3757 1820
Size: 174 391 27 1345 2298
Fragments arranged by size:
2298 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 761 1172 2258 2468 761
Size: 411 1086 210 2528
Fragments arranged by size:
2528 1086 411 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 622 2443 2753 2899 4045 622
Size: 1821 310 146 1146 812
Fragments arranged by size:
1821 1146 812 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 421 562 640 733 818 958 1114 2060
Size: 120 141 78 93 85 140 156 946
Cuts at: 2060 2492 3239 3507 301
Size: 432 747 268 1029
Fragments arranged by size:
1029 946 747 432 268 156 141 140
120 93 85 78
EcoNI CCTnn'n_nnAGG
Cuts at: 1051 1051
Size: 4235
EcoO109I rG'GnC_Cy
Cuts at: 186 360 411 1056 1374 186
Size: 174 51 645 318 3047
Fragments arranged by size:
3047 645 318 174 51
EcoRI G'AATT_C
Cuts at: 837 1239 837
Size: 402 3833
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2254 2254
Size: 4235
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 249 693 733 916 1669 1917 2939 3918 249
Size: 444 40 183 753 248 1022 979 566
Fragments arranged by size:
1022 979 753 566 444 248 183 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2077 2107 2109 2139 2077
Size: 30 2 30 4173
Fragments arranged by size:
4173 30 30 2
HincII GTy'rAC
Cuts at: 955 1274 955
Size: 319 3916
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 1255 1255
Size: 4235
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 752 972 2906 3090 752
Size: 220 1934 184 1897
Fragments arranged by size:
1934 1897 220 184
MscI TGG'CCA
Cuts at: 1996 1996
Size: 4235
MslI CAynn'nnrTG
Cuts at: 2224 2351 2224
Size: 127 4108
MspA1I CmG'CkG
Cuts at: 1346 1660 2020 3033 3278 1346
Size: 314 360 1013 245 2303
Fragments arranged by size:
2303 1013 360 314 245
NaeI GCC'GGC
Cuts at: 523 2416 523
Size: 1893 2342
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NcoI C'CATG_G
Cuts at: 107 107
Size: 4235
NgoMIV G'CCGG_C
Cuts at: 521 2414 521
Size: 1893 2342
NspI r_CATG'y
Cuts at: 543 1288 1788 2695 543
Size: 745 500 907 2083
Fragments arranged by size:
2083 907 745 500
PasI CC'CwG_GG
Cuts at: 1016 1016
Size: 4235
PciI A'CATG_T
Cuts at: 2691 2691
Size: 4235
PfoI T'CCnGG_A
Cuts at: 339 470 578 339
Size: 131 108 3996
Fragments arranged by size:
3996 131 108
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 4203
PpuMI rG'GwC_Cy
Cuts at: 186 186
Size: 4235
PshAI GACnn'nnGTC
Cuts at: 950 950
Size: 4235
PspOMI G'GGCC_C
Cuts at: 410 410
Size: 4235
PstI C_TGCA'G
Cuts at: 125 632 1282 1967 125
Size: 507 650 685 2393
Fragments arranged by size:
2393 685 650 507
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 1660 2020 1660
Size: 360 3875
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SanDI GG'GwC_CC
Cuts at: 186 186
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 1172 2258 2468 1172
Size: 1086 210 2939
Fragments arranged by size:
2939 1086 210
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SexAI A'CCwGG_T
Cuts at: 437 1131 437
Size: 694 3541
SfcI C'TryA_G
Cuts at: 33 121 558 628 1226 1278 1963 2956 3147
Size: 88 437 70 598 52 685 993 191
Cuts at: 3147 33
Size: 1121
Fragments arranged by size:
1121 993 685 598 437 191 88 70
52
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SgrAI Cr'CCGG_yG
Cuts at: 521 521
Size: 4235
SmaI CCC'GGG
Cuts at: 464 1257 464
Size: 793 3442
SmlI C'TyrA_G
Cuts at: 159 1390 2305 2797 3059 159
Size: 1231 915 492 262 1335
Fragments arranged by size:
1335 1231 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
SrfI GCCC'GGGC
Cuts at: 464 464
Size: 4235
StuI AGG'CCT
Cuts at: 1217 1217
Size: 4235
StyI C'CwwG_G
Cuts at: 107 1369 1723 107
Size: 1262 354 2619
Fragments arranged by size:
2619 1262 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 923 1818 1951 2410 2619 355
Size: 568 895 133 459 209 1971
Fragments arranged by size:
1971 895 568 459 209 133
TatI w'GTAC_w
Cuts at: 3750 3750
Size: 4235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4203
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 247 793 1730 247
Size: 546 937 2752
Fragments arranged by size:
2752 937 546
XmaI C'CCGG_G
Cuts at: 462 1255 462
Size: 793 3442
XmnI GAAnn'nnTTC
Cuts at: 116 706 116
Size: 590 3645
ZraI GAC'GTC
Cuts at: 378 378
Size: 4235
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI
BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PsiI
PspXI PsrI SacII SfiI SnaBI SspI SwaI XhoI