Restriction map for
pF1KB9849
(Circular) (Six-base) MAPSORT of: pF1KB9849.seq Check: 2633 from: 1 to: 4244
pF1KB9849 4244 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 490 490
Size: 4244
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 666 2069 2501 3248 3516 393
Size: 273 1403 432 747 268 1121
Fragments arranged by size:
1403 1121 747 432 273 268
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 497 2632 497
Size: 2135 2109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 448 508 853 448
Size: 60 345 3839
Fragments arranged by size:
3839 345 60
AlwNI CAG_nnn'CTG
Cuts at: 1549 3116 1549
Size: 1567 2677
ApaI G_GGCC'C
Cuts at: 127 549 127
Size: 422 3822
ApaLI G'TGCA_C
Cuts at: 493 3014 493
Size: 2521 1723
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 673 804 1058 1264 673
Size: 131 254 206 3653
Fragments arranged by size:
3653 254 206 131
AvrII C'CTAG_G
Cuts at: 817 817
Size: 4244
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 148 179 669 732 1260 1674 1922 1957 148
Size: 31 490 63 528 414 248 35 2435
Fragments arranged by size:
2435 528 490 414 248 63 35 31
BanII G_rGCy'C
Cuts at: 127 549 680 1258 2288 127
Size: 422 131 578 1030 2083
Fragments arranged by size:
2083 1030 578 422 131
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 858 858
Size: 4244
BbvCI CC'TCA_GC
Cuts at: 934 934
Size: 4244
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 601 1099 601
Size: 498 3746
BglII A'GATC_T
Cuts at: 1759 3796 1759
Size: 2037 2207
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 127 497 549 737 1869 1962 3018 127
Size: 370 52 188 1132 93 1056 1353
Fragments arranged by size:
1353 1132 1056 370 188 93 52
BmgBI CAC'GTC
Cuts at: 894 894
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1537 1872 4016 1456
Size: 81 335 2144 1684
Fragments arranged by size:
2144 1684 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
Bpu10I CC'TnA_GC
Cuts at: 934 934
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1420 2299 2791 3089 277
Size: 1143 879 492 298 1432
Fragments arranged by size:
1432 1143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 207 762 1597 207
Size: 555 835 2854
Fragments arranged by size:
2854 835 555
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 509 1675 1923 2625 4121 509
Size: 1166 248 702 1496 632
Fragments arranged by size:
1496 1166 702 632 248
BsaWI w'CCGG_w
Cuts at: 497 1433 1954 2632 2906 3053 3464 497
Size: 936 521 678 274 147 411 1277
Fragments arranged by size:
1277 936 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 168 536 714 1033 1084 1113 1669 2427 3004
Size: 368 178 319 51 29 556 758 577
Cuts at: 3004 3500 168
Size: 496 912
Fragments arranged by size:
912 758 577 556 496 368 319 178
51 29
BsiEI CG_ry'CG
Cuts at: 104 985 1786 1832 3040 104
Size: 881 801 46 1208 1308
Fragments arranged by size:
1308 1208 881 801 46
BsiHKAI G_wGCw'C
Cuts at: 422 497 614 680 767 1022 1258 2036 2226
Size: 75 117 66 87 255 236 778 190
Cuts at: 2226 3018 422
Size: 792 1648
Fragments arranged by size:
1648 792 778 255 236 190 117 87
75 66
BsiWI C'GTAC_G
Cuts at: 1047 1047
Size: 4244
BsmI GAATG_Cn'
Cuts at: 309 309
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 127 422 497 549 614 680 737 767 1022
Size: 295 75 52 65 66 57 30 255
Cuts at: 1022 1258 1869 1962 2036 2226 2288 3018 127
Size: 236 611 93 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 295 255 236 190 93
75 74 66 65 62 57 52 30
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 490 661 1294 1508 1810 2191 490
Size: 171 633 214 302 381 2543
Fragments arranged by size:
2543 633 381 302 214 171
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2156 2156
Size: 4244
BsrFI r'CCGG_y
Cuts at: 497 1203 2242 2423 2632 497
Size: 706 1039 181 209 2109
Fragments arranged by size:
2109 1039 706 209 181
BssHII G'CGCG_C
Cuts at: 632 634 632
Size: 2 4242
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 352 1621 352
Size: 1269 2975
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstEII G'GTnAC_C
Cuts at: 975 975
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 852 963 852
Size: 111 4133
BstYI r'GATC_y
Cuts at: 1 1126 1269 1736 1759 2094 2340 3352 3438
Size: 1125 143 467 23 335 246 1012 86
Cuts at: 3438 3450 3796 1
Size: 12 346 449
Fragments arranged by size:
1125 1012 467 449 346 335 246 143
86 23 12
BtgI C'CryG_G
Cuts at: 107 263 390 851 107
Size: 156 127 461 3500
Fragments arranged by size:
3500 461 156 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 831 2194 2375 2538 831
Size: 1363 181 163 2537
Fragments arranged by size:
2537 1363 181 163
BtsI GCAGTG_nn'
Cuts at: 1179 1487 1179
Size: 308 3936
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 566 982 989 1829 2003 2394 2421 3766 566
Size: 416 7 840 174 391 27 1345 1044
Fragments arranged by size:
1345 1044 840 416 391 174 27 7
EagI C'GGCC_G
Cuts at: 982 1829 982
Size: 847 3397
EarI CTCTTCn'nnn_
Cuts at: 448 478 685 730 2267 2477 448
Size: 30 207 45 1537 210 2215
Fragments arranged by size:
2215 1537 210 207 45 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1203 2452 2762 2908 4054 1203
Size: 1249 310 146 1146 1393
Fragments arranged by size:
1393 1249 1146 310 146
EcoICRI GAG'CTC
Cuts at: 678 1256 678
Size: 578 3666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 393 666 2069 2501 3248 3516 393
Size: 273 1403 432 747 268 1121
Fragments arranged by size:
1403 1121 747 432 273 268
EcoO109I rG'GnC_Cy
Cuts at: 124 603 800 1383 124
Size: 479 197 583 2985
Fragments arranged by size:
2985 583 479 197
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 841 1123 2025 841
Size: 282 902 3060
Fragments arranged by size:
3060 902 282
FspAI rTGC'GCAy
Cuts at: 841 841
Size: 4244
HaeII r_GCGC'y
Cuts at: 271 1678 1926 2948 3927 271
Size: 1407 248 1022 979 588
Fragments arranged by size:
1407 1022 979 588 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2086 2116 2118 2148 2086
Size: 30 2 30 4182
Fragments arranged by size:
4182 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1154 2915 3099 1154
Size: 1761 184 2299
Fragments arranged by size:
2299 1761 184
MscI TGG'CCA
Cuts at: 991 2005 991
Size: 1014 3230
MslI CAynn'nnrTG
Cuts at: 319 490 850 961 2233 2360 319
Size: 171 360 111 1272 127 2203
Fragments arranged by size:
2203 1272 360 171 127 111
MspA1I CmG'CkG
Cuts at: 369 392 938 987 1355 1669 2029 3042 3287
Size: 23 546 49 368 314 360 1013 245
Cuts at: 3287 369
Size: 1326
Fragments arranged by size:
1326 1013 546 368 360 314 245 49
23
NaeI GCC'GGC
Cuts at: 1205 2425 1205
Size: 1220 3024
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 107 107
Size: 4244
NgoMIV G'CCGG_C
Cuts at: 1203 2423 1203
Size: 1220 3024
NspI r_CATG'y
Cuts at: 1146 1297 1797 2704 1146
Size: 151 500 907 2686
Fragments arranged by size:
2686 907 500 151
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 1078 1078
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PpuMI rG'GwC_Cy
Cuts at: 800 800
Size: 4244
PspOMI G'GGCC_C
Cuts at: 123 545 123
Size: 422 3822
PstI C_TGCA'G
Cuts at: 466 1111 1291 1976 466
Size: 645 180 685 2734
Fragments arranged by size:
2734 685 645 180
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 938 1669 2029 938
Size: 731 360 3153
Fragments arranged by size:
3153 731 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 680 1258 680
Size: 578 3666
SacII CC_GC'GG
Cuts at: 393 393
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 685 730 2267 2477 685
Size: 45 1537 210 2452
Fragments arranged by size:
2452 1537 210 45
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 174 462 699 1107 1287 1972 2965 3156
Size: 141 288 237 408 180 685 993 191
Cuts at: 3156 33
Size: 1121
Fragments arranged by size:
1121 993 685 408 288 237 191 180
141
SfiI GGCCn_nnn'nGGCC
Cuts at: 601 601
Size: 4244
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SgrAI Cr'CCGG_yG
Cuts at: 497 497
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 292 1399 2314 2806 3068 292
Size: 1107 915 492 262 1468
Fragments arranged by size:
1468 1107 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StyI C'CwwG_G
Cuts at: 107 817 1378 1732 107
Size: 710 561 354 2619
Fragments arranged by size:
2619 710 561 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 570 1089 1827 1960 2419 2628 570
Size: 519 738 133 459 209 2186
Fragments arranged by size:
2186 738 519 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4212
Tth111I GACn'n_nGTC
Cuts at: 1194 2041 1194
Size: 847 3397
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1739 1739
Size: 4244
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI BaeI
BcgI BfrBI BmtI BpmI BsaXI BseRI BsgI BsmBI
BsrGI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SanDI SexAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI