Restriction map for
pF1KB9850
(Circular) (Six-base) MAPSORT of: pF1KB9850.seq Check: 8086 from: 1 to: 4244
pF1KB9850 4244 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2069 2501 3248 3516 2069
Size: 432 747 268 2797
Fragments arranged by size:
2797 747 432 268
AfeI AGC'GCT
Cuts at: 820 820
Size: 4244
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 518 850 1549 3116 518
Size: 332 699 1567 1646
Fragments arranged by size:
1646 1567 699 332
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 989 1264 989
Size: 275 3969
BamHI G'GATC_C
Cuts at: 195 1103 1269 195
Size: 908 166 3170
Fragments arranged by size:
3170 908 166
BanI G'GyrC_C
Cuts at: 1260 1674 1922 1957 1260
Size: 414 248 35 3547
Fragments arranged by size:
3547 414 248 35
BanII G_rGCy'C
Cuts at: 1258 2288 1258
Size: 1030 3214
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglII A'GATC_T
Cuts at: 221 689 1759 3796 221
Size: 468 1070 2037 669
Fragments arranged by size:
2037 1070 669 468
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 899 963 1869 1962 3018 899
Size: 64 906 93 1056 2125
Fragments arranged by size:
2125 1056 906 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 528 1456 1537 1872 4016 528
Size: 928 81 335 2144 756
Fragments arranged by size:
2144 928 756 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 915 2611 915
Size: 1696 2548
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 864 193
Size: 671 3573
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1420 2299 2791 3089 202
Size: 1218 879 492 298 1357
Fragments arranged by size:
1357 1218 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 467 936 1597 467
Size: 469 661 3114
Fragments arranged by size:
3114 661 469
BsaBI GATnn'nnATC
Cuts at: 6 1758 3795 6
Size: 1752 2037 455
Fragments arranged by size:
2037 1752 455
BsaHI Gr'CG_yC
Cuts at: 1675 1923 2625 4121 1675
Size: 248 702 1496 1798
Fragments arranged by size:
1798 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1433 1954 2632 2906 3053 3464 1226
Size: 207 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 207 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 646 676 646
Size: 30 4214
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 670
Size: 4244
BseYI C'CCAG_C
Cuts at: 1669 2427 3004 3500 1669
Size: 758 577 496 2413
Fragments arranged by size:
2413 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 456 456
Size: 4244
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1258 2036 2226 3018 1258
Size: 778 190 792 2484
Fragments arranged by size:
2484 792 778 190
BsmI GAATG_Cn'
Cuts at: 174 174
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 899 963 1258 1869 1962 2036 2226 2288 3018
Size: 64 295 611 93 74 190 62 730
Cuts at: 3018 899
Size: 2125
Fragments arranged by size:
2125 730 611 295 190 93 74 64
62
BspEI T'CCGG_A
Cuts at: 1226 3464 1226
Size: 2238 2006
BspHI T'CATG_A
Cuts at: 3420 3420
Size: 4244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 627 2536 2590 4074 4239 50
Size: 577 1909 54 1484 165 55
Fragments arranged by size:
1909 1484 577 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 2156 500
Size: 1656 2588
BsrFI r'CCGG_y
Cuts at: 2242 2423 2632 2242
Size: 181 209 3854
Fragments arranged by size:
3854 209 181
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 1621
Size: 4244
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstYI r'GATC_y
Cuts at: 1 160 195 221 689 764 1103 1269 1736
Size: 159 35 26 468 75 339 166 467
Cuts at: 1736 1759 2094 2340 3352 3438 3450 3796 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 468 467 449 346 339 335 246
166 159 86 75 35 26 23 12
BtgI C'CryG_G
Cuts at: 233 827 233
Size: 594 3650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 378 2194 2375 2538 378
Size: 1816 181 163 2084
Fragments arranged by size:
2084 1816 181 163
BtsI GCAGTG_nn'
Cuts at: 1487 1487
Size: 4244
DraI TTT'AAA
Cuts at: 1148 1243 1148
Size: 95 4149
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 257 347 426 564 572 759 1829 2003 2394
Size: 90 79 138 8 187 1070 174 391
Cuts at: 2394 2421 3766 257
Size: 27 1345 735
Fragments arranged by size:
1345 1070 735 391 187 174 138 90
79 27 8
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 634 1184 2267 2477 634
Size: 550 1083 210 2401
Fragments arranged by size:
2401 1083 550 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2452 2762 2908 4054 2452
Size: 310 146 1146 2642
Fragments arranged by size:
2642 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 864 2069 2501 3248 3516 193
Size: 671 1205 432 747 268 921
Fragments arranged by size:
1205 921 747 671 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 142 889 142
Size: 747 3497
EcoO109I rG'GnC_Cy
Cuts at: 203 719 857 1031 1383 203
Size: 516 138 174 352 3064
Fragments arranged by size:
3064 516 352 174 138
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 217 822 1117 1678 1926 2948 3927 217
Size: 605 295 561 248 1022 979 534
Fragments arranged by size:
1022 979 605 561 534 295 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 646 678 2086 2116 2118 2148 646
Size: 32 1408 30 2 30 2742
Fragments arranged by size:
2742 1408 32 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
HindIII A'AGCT_T
Cuts at: 946 1202 946
Size: 256 3988
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 179 644 2915 3099 179
Size: 465 2271 184 1324
Fragments arranged by size:
2271 1324 465 184
MscI TGG'CCA
Cuts at: 259 349 428 566 2005 259
Size: 90 79 138 1439 2498
Fragments arranged by size:
2498 1439 138 90 79
MslI CAynn'nnrTG
Cuts at: 494 2233 2360 494
Size: 1739 127 2378
Fragments arranged by size:
2378 1739 127
MspA1I CmG'CkG
Cuts at: 1355 1669 2029 3042 3287 1355
Size: 314 360 1013 245 2312
Fragments arranged by size:
2312 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2425 2425
Size: 4244
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NgoMIV G'CCGG_C
Cuts at: 2423 2423
Size: 4244
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PflMI CCAn_nnn'nTGG
Cuts at: 593 593
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PmlI CAC'GTG
Cuts at: 467 936 467
Size: 469 3775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PpuMI rG'GwC_Cy
Cuts at: 203 857 203
Size: 654 3590
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 1669 2029 1669
Size: 360 3884
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 634 2267 2477 634
Size: 1633 210 2401
Fragments arranged by size:
2401 1633 210
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 1287 1972 2965 3156 33
Size: 1254 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 217 989 1399 2314 2806 3068 217
Size: 772 410 915 492 262 1393
Fragments arranged by size:
1393 915 772 492 410 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StuI AGG'CCT
Cuts at: 137 151 1234 137
Size: 14 1083 3147
Fragments arranged by size:
3147 1083 14
StyI C'CwwG_G
Cuts at: 644 1378 1732 644
Size: 734 354 3156
Fragments arranged by size:
3156 734 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 424 1827 1960 2419 2628 424
Size: 1403 133 459 209 2040
Fragments arranged by size:
2040 1403 459 209 133
TatI w'GTAC_w
Cuts at: 282 3759 282
Size: 3477 767
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4212
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 1011 1739 360
Size: 651 728 2865
Fragments arranged by size:
2865 728 651
XhoI C'TCGA_G
Cuts at: 989 989
Size: 4244
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
XmnI GAAnn'nnTTC
Cuts at: 137 820 1193 137
Size: 683 373 3188
Fragments arranged by size:
3188 683 373
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI