Restriction map for
pF1KB9851
(Circular) (Six-base) MAPSORT of: pF1KB9851.seq Check: 3100 from: 1 to: 4253
pF1KB9851 4253 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1291 1291
Size: 4253
Acc65I G'GTAC_C
Cuts at: 1269 1269
Size: 4253
AclI AA'CG_TT
Cuts at: 4093 4093
Size: 4253
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1145 2078 2510 3257 3525 1015
Size: 130 933 432 747 268 1743
Fragments arranged by size:
1743 933 747 432 268 130
AflIII A'CryG_T
Cuts at: 1605 2709 3686 1605
Size: 1104 977 2172
Fragments arranged by size:
2172 1104 977
AgeI A'CCGG_T
Cuts at: 2641 2641
Size: 4253
AhdI GACnn_n'nnGTC
Cuts at: 981 981
Size: 4253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 553 688 553
Size: 135 4118
AlwNI CAG_nnn'CTG
Cuts at: 234 753 985 1558 3125 234
Size: 519 232 573 1567 1362
Fragments arranged by size:
1567 1362 573 519 232
ApaI G_GGCC'C
Cuts at: 836 836
Size: 4253
ApaLI G'TGCA_C
Cuts at: 3023 3023
Size: 4253
ApoI r'AATT_y
Cuts at: 113 481 724 1257 3606 3862 113
Size: 368 243 533 2349 256 504
Fragments arranged by size:
2349 533 504 368 256 243
AseI AT'TA_AT
Cuts at: 20 20
Size: 4253
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
AvaI C'yCGr_G
Cuts at: 1273 1273
Size: 4253
BamHI G'GATC_C
Cuts at: 1192 1278 1192
Size: 86 4167
BanI G'GyrC_C
Cuts at: 1269 1683 1931 1966 1269
Size: 414 248 35 3556
Fragments arranged by size:
3556 414 248 35
BanII G_rGCy'C
Cuts at: 836 1267 2297 836
Size: 431 1030 2792
Fragments arranged by size:
2792 1030 431
BbeI G_GCGC'C
Cuts at: 1687 1935 1687
Size: 248 4005
BbvCI CC'TCA_GC
Cuts at: 422 422
Size: 4253
BciVI GTATCCnnnnn_n'
Cuts at: 829 2151 2912 4253 829
Size: 1322 761 1341 829
Fragments arranged by size:
1341 1322 829 761
BclI T'GATC_A
Cuts at: 1025 1773 1025
Size: 748 3505
BglI GCCn_nnn'nGGC
Cuts at: 551 1106 551
Size: 555 3698
BglII A'GATC_T
Cuts at: 1768 3805 1768
Size: 2037 2216
BlpI GC'TnA_GC
Cuts at: 541 1166 1365 541
Size: 625 199 3429
Fragments arranged by size:
3429 625 199
Bme1580I G_kGCm'C
Cuts at: 836 1878 1971 3027 836
Size: 1042 93 1056 2062
Fragments arranged by size:
2062 1056 1042 93
BmrI ACTGGGnnnn_n'
Cuts at: 146 569 1226 1465 1546 1881 4025 146
Size: 423 657 239 81 335 2144 374
Fragments arranged by size:
2144 657 423 374 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4253
Bpu10I CC'TnA_GC
Cuts at: 422 449 733 422
Size: 27 284 3942
Fragments arranged by size:
3942 284 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1429 2308 2800 3098 367
Size: 1062 879 492 298 1522
Fragments arranged by size:
1522 1062 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 277 3957 277
Size: 3680 573
BsaAI yAC'GTr
Cuts at: 1606 1606
Size: 4253
BsaBI GATnn'nnATC
Cuts at: 6 1767 3804 6
Size: 1761 2037 455
Fragments arranged by size:
2037 1761 455
BsaHI Gr'CG_yC
Cuts at: 1684 1932 2634 4130 1684
Size: 248 702 1496 1807
Fragments arranged by size:
1807 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1442 1963 2641 2915 3062 3473 1442
Size: 521 678 274 147 411 2222
Fragments arranged by size:
2222 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 982 1012 982
Size: 30 4223
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 892 1075 289
Size: 603 183 3467
Fragments arranged by size:
3467 603 183
BseYI C'CCAG_C
Cuts at: 457 753 1678 2436 3013 3509 457
Size: 296 925 758 577 496 1201
Fragments arranged by size:
1201 925 758 577 496 296
BsiEI CG_ry'CG
Cuts at: 104 1795 1841 3049 104
Size: 1691 46 1208 1308
Fragments arranged by size:
1691 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1267 2045 2235 3027 1267
Size: 778 190 792 2493
Fragments arranged by size:
2493 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 836 1267 1878 1971 2045 2235 2297 3027 836
Size: 431 611 93 74 190 62 730 2062
Fragments arranged by size:
2062 730 611 431 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3473 3473
Size: 4253
BspHI T'CATG_A
Cuts at: 3429 3429
Size: 4253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1303 1517 1819 2200 1303
Size: 214 302 381 3356
Fragments arranged by size:
3356 381 302 214
BsrBI CCG'CTC
Cuts at: 50 414 2545 2599 4083 4248 50
Size: 364 2131 54 1484 165 55
Fragments arranged by size:
2131 1484 364 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2165 2165
Size: 4253
BsrFI r'CCGG_y
Cuts at: 1147 2251 2432 2641 1147
Size: 1104 181 209 2759
Fragments arranged by size:
2759 1104 209 181
BssSI C'ACGA_G
Cuts at: 2524 2882 2524
Size: 358 3895
BstAPI GCAn_nnn'nTGC
Cuts at: 1630 1630
Size: 4253
BstBI TT'CG_AA
Cuts at: 2614 2614
Size: 4253
BstEII G'GTnAC_C
Cuts at: 1143 1143
Size: 4253
BstXI CCAn_nnnn'nTGG
Cuts at: 926 926
Size: 4253
BstYI r'GATC_y
Cuts at: 1 780 1192 1278 1745 1768 2103 2349 3361
Size: 779 412 86 467 23 335 246 1012
Cuts at: 3361 3447 3459 3805 1
Size: 86 12 346 449
Fragments arranged by size:
1012 779 467 449 412 346 335 246
86 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 965 965
Size: 4253
BtgI C'CryG_G
Cuts at: 107 107
Size: 4253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2203 2384 2547 2203
Size: 181 163 3909
Fragments arranged by size:
3909 181 163
BtsI GCAGTG_nn'
Cuts at: 414 807 1080 1496 414
Size: 393 273 416 3171
Fragments arranged by size:
3171 416 393 273
DraI TTT'AAA
Cuts at: 1236 1252 1236
Size: 16 4237
DraIII CAC_nnn'GTG
Cuts at: 577 577
Size: 4253
DrdI GACnn_nn'nnGTC
Cuts at: 1959 2817 1959
Size: 858 3395
EaeI y'GGCC_r
Cuts at: 125 189 200 398 1838 2012 2403 2430 3775
Size: 64 11 198 1440 174 391 27 1345
Cuts at: 3775 125
Size: 603
Fragments arranged by size:
1440 1345 603 391 198 174 64 27
11
EagI C'GGCC_G
Cuts at: 1838 1838
Size: 4253
EarI CTCTTCn'nnn_
Cuts at: 958 1013 1164 2276 2486 958
Size: 55 151 1112 210 2725
Fragments arranged by size:
2725 1112 210 151 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2461 2771 2917 4063 2461
Size: 310 146 1146 2651
Fragments arranged by size:
2651 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1265 1265
Size: 4253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1145 2078 2510 3257 3525 1015
Size: 130 933 432 747 268 1743
Fragments arranged by size:
1743 933 747 432 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 607 763 607
Size: 156 4097
EcoO109I rG'GnC_Cy
Cuts at: 1392 1392
Size: 4253
EcoRI G'AATT_C
Cuts at: 113 724 1257 113
Size: 611 533 3109
Fragments arranged by size:
3109 611 533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 201 2272 201
Size: 2071 2182
FspI TGC'GCA
Cuts at: 2034 2034
Size: 4253
HaeII r_GCGC'y
Cuts at: 1687 1935 2957 3936 1687
Size: 248 1022 979 2004
Fragments arranged by size:
2004 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 967 999 2095 2125 2127 2157 210
Size: 32 725 32 1096 30 2 30 2306
Fragments arranged by size:
2306 1096 725 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1292 1292
Size: 4253
KasI G'GCGC_C
Cuts at: 1683 1931 1683
Size: 248 4005
KpnI G_GTAC'C
Cuts at: 1273 1273
Size: 4253
MluI A'CGCG_T
Cuts at: 3686 3686
Size: 4253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2924 3108 2924
Size: 184 4069
MscI TGG'CCA
Cuts at: 127 202 400 2014 127
Size: 75 198 1614 2366
Fragments arranged by size:
2366 1614 198 75
MslI CAynn'nnrTG
Cuts at: 325 521 2242 2369 325
Size: 196 1721 127 2209
Fragments arranged by size:
2209 1721 196 127
MspA1I CmG'CkG
Cuts at: 438 593 1364 1678 2038 3051 3296 438
Size: 155 771 314 360 1013 245 1395
Fragments arranged by size:
1395 1013 771 360 314 245 155
NaeI GCC'GGC
Cuts at: 2434 2434
Size: 4253
NarI GG'CG_CC
Cuts at: 1684 1932 1684
Size: 248 4005
NcoI C'CATG_G
Cuts at: 107 107
Size: 4253
NgoMIV G'CCGG_C
Cuts at: 2432 2432
Size: 4253
NspI r_CATG'y
Cuts at: 309 1306 1806 2713 309
Size: 997 500 907 1849
Fragments arranged by size:
1849 997 907 500
PasI CC'CwG_GG
Cuts at: 1116 1116
Size: 4253
PciI A'CATG_T
Cuts at: 2709 2709
Size: 4253
PflMI CCAn_nnn'nTGG
Cuts at: 300 300
Size: 4253
PfoI T'CCnGG_A
Cuts at: 161 719 161
Size: 558 3695
PmeI GTTT'AAAC
Cuts at: 1252 1252
Size: 4253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4221
PspOMI G'GGCC_C
Cuts at: 832 832
Size: 4253
PstI C_TGCA'G
Cuts at: 269 443 749 1300 1985 269
Size: 174 306 551 685 2537
Fragments arranged by size:
2537 685 551 306 174
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4253
PvuII CAG'CTG
Cuts at: 438 593 1678 2038 438
Size: 155 1085 360 2653
Fragments arranged by size:
2653 1085 360 155
RsrII CG'GwC_CG
Cuts at: 2448 2448
Size: 4253
SacI G_AGCT'C
Cuts at: 1267 1267
Size: 4253
SalI G'TCGA_C
Cuts at: 1290 1290
Size: 4253
SapI GCTCTTCn'nnn_
Cuts at: 958 1013 2276 2486 958
Size: 55 1263 210 2725
Fragments arranged by size:
2725 1263 210 55
SbfI CC_TGCA'GG
Cuts at: 1300 1300
Size: 4253
ScaI AGT'ACT
Cuts at: 3770 3770
Size: 4253
SfcI C'TryA_G
Cuts at: 33 265 439 745 1296 1981 2974 3165 33
Size: 232 174 306 551 685 993 191 1121
Fragments arranged by size:
1121 993 685 551 306 232 191 174
SfoI GGC'GCC
Cuts at: 1685 1933 1685
Size: 248 4005
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4253
SmaI CCC'GGG
Cuts at: 1275 1275
Size: 4253
SmlI C'TyrA_G
Cuts at: 382 1408 2323 2815 3077 382
Size: 1026 915 492 262 1558
Fragments arranged by size:
1558 1026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4253
SphI G_CATG'C
Cuts at: 1306 1806 1306
Size: 500 3753
StuI AGG'CCT
Cuts at: 122 122
Size: 4253
StyI C'CwwG_G
Cuts at: 107 1104 1187 1387 1741 107
Size: 997 83 200 354 2619
Fragments arranged by size:
2619 997 354 200 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 588 1129 1836 1969 2428 2637 588
Size: 541 707 133 459 209 2204
Fragments arranged by size:
2204 707 541 459 209 133
TatI w'GTAC_w
Cuts at: 3768 3768
Size: 4253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 982 1014 3770 3802 982
Size: 32 2756 32 1433
Fragments arranged by size:
2756 1433 32 32
Tth111I GACn'n_nGTC
Cuts at: 2050 2050
Size: 4253
XbaI T'CTAG_A
Cuts at: 1284 1284
Size: 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 327 843 1748 297
Size: 30 516 905 2802
Fragments arranged by size:
2802 905 516 30
XmaI C'CCGG_G
Cuts at: 1273 1273
Size: 4253
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BpmI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI