Restriction map for
pF1KB9853
(Circular) (Six-base) MAPSORT of: pF1KB9853.seq Check: 6405 from: 1 to: 4268
pF1KB9853 4268 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 894 1306 894
Size: 412 3856
Acc65I G'GTAC_C
Cuts at: 1284 1284
Size: 4268
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 2093 2525 3272 3540 970
Size: 1123 432 747 268 1698
Fragments arranged by size:
1698 1123 747 432 268
AflIII A'CryG_T
Cuts at: 1620 2724 3701 1620
Size: 1104 977 2187
Fragments arranged by size:
2187 1104 977
AgeI A'CCGG_T
Cuts at: 2656 2656
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 558 1573 3140 558
Size: 1015 1567 1686
Fragments arranged by size:
1686 1567 1015
ApaLI G'TGCA_C
Cuts at: 3038 3038
Size: 4268
ApoI r'AATT_y
Cuts at: 290 674 771 802 836 1037 1119 1202 1272
Size: 384 97 31 34 201 82 83 70
Cuts at: 1272 3621 3877 290
Size: 2349 256 681
Fragments arranged by size:
2349 681 384 256 201 97 83 82
70 34 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 1288 1288
Size: 4268
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 370 1242 1284 1698 1946 1981 370
Size: 872 42 414 248 35 2657
Fragments arranged by size:
2657 872 414 248 42 35
BanII G_rGCy'C
Cuts at: 1282 2312 1282
Size: 1030 3238
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 364 398 364
Size: 34 4234
BciVI GTATCCnnnnn_n'
Cuts at: 2166 2927 4268 2166
Size: 761 1341 2166
Fragments arranged by size:
2166 1341 761
BclI T'GATC_A
Cuts at: 1788 1788
Size: 4268
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 1380 1380
Size: 4268
Bme1580I G_kGCm'C
Cuts at: 1893 1986 3042 1893
Size: 93 1056 3119
Fragments arranged by size:
3119 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 129 1480 1561 1896 4040 129
Size: 1351 81 335 2144 357
Fragments arranged by size:
2144 1351 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 826 793
Size: 33 4235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 2815 3113 1444
Size: 879 492 298 2599
Fragments arranged by size:
2599 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1148 1782 3819 6
Size: 1142 634 2037 455
Fragments arranged by size:
2037 1142 634 455
BsaHI Gr'CG_yC
Cuts at: 1063 1699 1947 2649 4145 1063
Size: 636 248 702 1496 1186
Fragments arranged by size:
1496 1186 702 636 248
BsaWI w'CCGG_w
Cuts at: 1457 1978 2656 2930 3077 3488 1457
Size: 521 678 274 147 411 2237
Fragments arranged by size:
2237 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1693 2451 3028 3524 1693
Size: 758 577 496 2437
Fragments arranged by size:
2437 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 608 608
Size: 4268
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3064 104
Size: 1706 46 1208 1308
Fragments arranged by size:
1706 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1282 2060 2250 3042 1282
Size: 778 190 792 2508
Fragments arranged by size:
2508 792 778 190
BsmI GAATG_Cn'
Cuts at: 1019 1019
Size: 4268
BsmBI CGTCTCn'nnnn_
Cuts at: 1054 1054
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 1282 1893 1986 2060 2250 2312 3042 1282
Size: 611 93 74 190 62 730 2508
Fragments arranged by size:
2508 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3488 3488
Size: 4268
BspHI T'CATG_A
Cuts at: 942 3444 942
Size: 2502 1766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1318 1532 1834 2215 1318
Size: 214 302 381 3371
Fragments arranged by size:
3371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 2266 2447 2656 2266
Size: 181 209 3878
Fragments arranged by size:
3878 209 181
BssSI C'ACGA_G
Cuts at: 2539 2897 2539
Size: 358 3910
BstAPI GCAn_nnn'nTGC
Cuts at: 1645 1645
Size: 4268
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 399 399
Size: 4268
BstYI r'GATC_y
Cuts at: 1 130 1293 1760 1783 2118 2364 3376 3462
Size: 129 1163 467 23 335 246 1012 86
Cuts at: 3462 3474 3820 1
Size: 12 346 449
Fragments arranged by size:
1163 1012 467 449 346 335 246 129
86 23 12
BstZ17I GTA'TAC
Cuts at: 895 895
Size: 4268
Bsu36I CC'TnA_GG
Cuts at: 1125 1125
Size: 4268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2218 2399 2562 2218
Size: 181 163 3924
Fragments arranged by size:
3924 181 163
BtsI GCAGTG_nn'
Cuts at: 1511 1511
Size: 4268
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 233 268 233
Size: 35 4233
DraI TTT'AAA
Cuts at: 930 1267 930
Size: 337 3931
DrdI GACnn_nn'nnGTC
Cuts at: 1974 2832 1974
Size: 858 3410
EaeI y'GGCC_r
Cuts at: 1853 2027 2418 2445 3790 1853
Size: 174 391 27 1345 2331
Fragments arranged by size:
2331 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 482 1001 2291 2501 482
Size: 519 1290 210 2249
Fragments arranged by size:
2249 1290 519 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2476 2786 2932 4078 2476
Size: 310 146 1146 2666
Fragments arranged by size:
2666 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 826 970 2093 2525 3272 3540 793
Size: 33 144 1123 432 747 268 1521
Fragments arranged by size:
1521 1123 747 432 268 144 33
EcoO109I rG'GnC_Cy
Cuts at: 1407 1407
Size: 4268
EcoRI G'AATT_C
Cuts at: 674 1119 1272 674
Size: 445 153 3670
Fragments arranged by size:
3670 445 153
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 886 2287 886
Size: 1401 2867
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 1702 1950 2972 3951 1702
Size: 248 1022 979 2019
Fragments arranged by size:
2019 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 2110 2140 2142 2172 211
Size: 32 1867 30 2 30 2307
Fragments arranged by size:
2307 1867 32 30 30 2
HincII GTy'rAC
Cuts at: 279 444 1307 279
Size: 165 863 3240
Fragments arranged by size:
3240 863 165
HpaI GTT'AAC
Cuts at: 444 444
Size: 4268
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 1288 1288
Size: 4268
MfeI C'AATT_G
Cuts at: 419 419
Size: 4268
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 457 2939 3123 457
Size: 2482 184 1602
Fragments arranged by size:
2482 1602 184
MscI TGG'CCA
Cuts at: 2029 2029
Size: 4268
MslI CAynn'nnrTG
Cuts at: 2257 2384 2257
Size: 127 4141
MspA1I CmG'CkG
Cuts at: 439 622 1379 1693 2053 3066 3311 439
Size: 183 757 314 360 1013 245 1396
Fragments arranged by size:
1396 1013 757 360 314 245 183
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 4268
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 4268
NspI r_CATG'y
Cuts at: 203 1321 1821 2728 203
Size: 1118 500 907 1743
Fragments arranged by size:
1743 1118 907 500
PciI A'CATG_T
Cuts at: 2724 2724
Size: 4268
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4268
PfoI T'CCnGG_A
Cuts at: 809 809
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3407 3439 3407
Size: 32 4236
PstI C_TGCA'G
Cuts at: 145 1315 2000 145
Size: 1170 685 2413
Fragments arranged by size:
2413 1170 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 622 1693 2053 622
Size: 1071 360 2837
Fragments arranged by size:
2837 1071 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 145 1315 145
Size: 1170 3098
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 141 386 426 1131 1311 1996 2989 3180
Size: 108 245 40 705 180 685 993 191
Cuts at: 3180 33
Size: 1121
Fragments arranged by size:
1121 993 705 685 245 191 180 108
40
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 1423 2338 2830 3092 1423
Size: 915 492 262 2599
Fragments arranged by size:
2599 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 203 1321 1821 203
Size: 1118 500 2650
Fragments arranged by size:
2650 1118 500
SspI AAT'ATT
Cuts at: 354 354
Size: 4268
StyI C'CwwG_G
Cuts at: 1402 1756 1402
Size: 354 3914
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 463 1851 1984 2443 2652 463
Size: 1388 133 459 209 2079
Fragments arranged by size:
2079 1388 459 209 133
TatI w'GTAC_w
Cuts at: 1095 1100 3783 1095
Size: 5 2683 1580
Fragments arranged by size:
2683 1580 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4236
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 693 1299 693
Size: 606 3662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1763 1763
Size: 4268
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsrGI BssHII
BstEII BtgI ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII NcoI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI