Restriction map for
pF1KB9856
(Circular) (Six-base) MAPSORT of: pF1KB9856.seq Check: 4264 from: 1 to: 4283
pF1KB9856 4283 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 737 1321 737
Size: 584 3699
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2540 3287 3555 2108
Size: 432 747 268 2836
Fragments arranged by size:
2836 747 432 268
AfeI AGC'GCT
Cuts at: 856 856
Size: 4283
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AleI CACnn'nnGTG
Cuts at: 527 527
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 1588 3155 1588
Size: 1567 2716
ApaLI G'TGCA_C
Cuts at: 1269 3053 1269
Size: 1784 2499
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 863 889 1303 863
Size: 26 414 3843
Fragments arranged by size:
3843 414 26
AvrII C'CTAG_G
Cuts at: 1053 1053
Size: 4283
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 1299 1713 1961 1996 1299
Size: 414 248 35 3586
Fragments arranged by size:
3586 414 248 35
BanII G_rGCy'C
Cuts at: 154 870 1297 2327 154
Size: 716 427 1030 2110
Fragments arranged by size:
2110 1030 716 427
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 624 624
Size: 4283
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BglI GCCn_nnn'nGGC
Cuts at: 379 379
Size: 4283
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 941 1273 1908 2001 3057 941
Size: 332 635 93 1056 2167
Fragments arranged by size:
2167 1056 635 332 93
BmgBI CAC'GTC
Cuts at: 899 936 899
Size: 37 4246
BmrI ACTGGGnnnn_n'
Cuts at: 466 561 1495 1576 1911 4055 466
Size: 95 934 81 335 2144 694
Fragments arranged by size:
2144 934 694 335 95 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
Bpu10I CC'TnA_GC
Cuts at: 677 677
Size: 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 1459 2338 2830 3128 155
Size: 1304 879 492 298 1310
Fragments arranged by size:
1310 1304 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 151 3987 151
Size: 3836 447
BsaAI yAC'GTr
Cuts at: 804 1636 804
Size: 832 3451
BsaBI GATnn'nnATC
Cuts at: 6 418 1120 1797 3834 6
Size: 412 702 677 2037 455
Fragments arranged by size:
2037 702 677 455 412
BsaHI Gr'CG_yC
Cuts at: 566 669 835 995 1714 1962 2664 4160 566
Size: 103 166 160 719 248 702 1496 689
Fragments arranged by size:
1496 719 702 689 248 166 160 103
BsaWI w'CCGG_w
Cuts at: 332 949 1472 1993 2671 2945 3092 3503 332
Size: 617 523 521 678 274 147 411 1112
Fragments arranged by size:
1112 678 617 523 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 223 247 253 217
Size: 6 24 6 4247
Fragments arranged by size:
4247 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 766 703
Size: 63 4220
BseYI C'CCAG_C
Cuts at: 869 940 1176 1708 2466 3043 3539 869
Size: 71 236 532 758 577 496 1613
Fragments arranged by size:
1613 758 577 532 496 236 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 505 331
Size: 174 4109
BsiEI CG_ry'CG
Cuts at: 104 447 1825 1871 3079 104
Size: 343 1378 46 1208 1308
Fragments arranged by size:
1378 1308 1208 343 46
BsiHKAI G_wGCw'C
Cuts at: 126 1273 1297 2075 2265 3057 126
Size: 1147 24 778 190 792 1352
Fragments arranged by size:
1352 1147 792 778 190 24
BsmI GAATG_Cn'
Cuts at: 706 1243 706
Size: 537 3746
Bsp1286I G_dGCh'C
Cuts at: 126 154 870 941 1273 1297 1908 2001 2075
Size: 28 716 71 332 24 611 93 74
Cuts at: 2075 2265 2327 3057 126
Size: 190 62 730 1352
Fragments arranged by size:
1352 730 716 611 332 190 93 74
71 62 28 24
BspEI T'CCGG_A
Cuts at: 949 3503 949
Size: 2554 1729
BspHI T'CATG_A
Cuts at: 611 3459 611
Size: 2848 1435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 1333 1547 1849 2230 401
Size: 932 214 302 381 2454
Fragments arranged by size:
2454 932 381 302 214
BsrBI CCG'CTC
Cuts at: 50 815 2575 2629 4113 4278 50
Size: 765 1760 54 1484 165 55
Fragments arranged by size:
1760 1484 765 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 442 847 2281 2462 2671 442
Size: 405 1434 181 209 2054
Fragments arranged by size:
2054 1434 405 209 181
BsrGI T'GTAC_A
Cuts at: 431 431
Size: 4283
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstEII G'GTnAC_C
Cuts at: 384 906 384
Size: 522 3761
BstYI r'GATC_y
Cuts at: 1 952 1308 1775 1798 2133 2379 3391 3477
Size: 951 356 467 23 335 246 1012 86
Cuts at: 3477 3489 3835 1
Size: 12 346 449
Fragments arranged by size:
1012 951 467 449 356 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 146 569 743 107
Size: 39 423 174 3647
Fragments arranged by size:
3647 423 174 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 519 765 2233 2414 2577 519
Size: 246 1468 181 163 2225
Fragments arranged by size:
2225 1468 246 181 163
BtsI GCAGTG_nn'
Cuts at: 506 1526 506
Size: 1020 3263
DraI TTT'AAA
Cuts at: 1236 1282 1236
Size: 46 4237
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 290 444 792 1022 1868 2042 2433 2460 3805
Size: 154 348 230 846 174 391 27 1345
Cuts at: 3805 290
Size: 768
Fragments arranged by size:
1345 846 768 391 348 230 174 154
27
EagI C'GGCC_G
Cuts at: 444 1868 444
Size: 1424 2859
EarI CTCTTCn'nnn_
Cuts at: 680 2306 2516 680
Size: 1626 210 2447
Fragments arranged by size:
2447 1626 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1295 1295
Size: 4283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 2540 3287 3555 2108
Size: 432 747 268 2836
Fragments arranged by size:
2836 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1422 1422
Size: 4283
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 212 280 858 1717 1965 2987 3966 212
Size: 68 578 859 248 1022 979 529
Fragments arranged by size:
1022 979 859 578 529 248 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 223 255 360 392 679 711 2125 2155 2157
Size: 32 105 32 287 32 1414 30 2
Cuts at: 2157 2187 223
Size: 30 2319
Fragments arranged by size:
2319 1414 287 105 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 470 677 1016 2954 3138 470
Size: 207 339 1938 184 1615
Fragments arranged by size:
1938 1615 339 207 184
MscI TGG'CCA
Cuts at: 292 2044 292
Size: 1752 2531
MslI CAynn'nnrTG
Cuts at: 409 527 2272 2399 409
Size: 118 1745 127 2293
Fragments arranged by size:
2293 1745 127 118
MspA1I CmG'CkG
Cuts at: 148 449 873 1394 1708 2068 3081 3326 148
Size: 301 424 521 314 360 1013 245 1105
Fragments arranged by size:
1105 1013 521 424 360 314 301 245
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 107 107
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 1336 1836 2743 1336
Size: 500 907 2876
Fragments arranged by size:
2876 907 500
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PfoI T'CCnGG_A
Cuts at: 817 817
Size: 4283
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PstI C_TGCA'G
Cuts at: 208 1330 2015 208
Size: 1122 685 2476
Fragments arranged by size:
2476 1122 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 873 1708 2068 873
Size: 835 360 3088
Fragments arranged by size:
3088 835 360
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1297 1297
Size: 4283
SacII CC_GC'GG
Cuts at: 149 149
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 257 577 3800 257
Size: 320 3223 740
Fragments arranged by size:
3223 740 320
SfcI C'TryA_G
Cuts at: 33 204 1326 2011 3004 3195 33
Size: 171 1122 685 993 191 1121
Fragments arranged by size:
1122 1121 993 685 191 171
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 891 1305 891
Size: 414 3869
SmlI C'TyrA_G
Cuts at: 170 1438 2353 2845 3107 170
Size: 1268 915 492 262 1346
Fragments arranged by size:
1346 1268 915 492 262
SnaBI TAC'GTA
Cuts at: 804 804
Size: 4283
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
StyI C'CwwG_G
Cuts at: 107 212 287 1053 1417 1771 107
Size: 105 75 766 364 354 2619
Fragments arranged by size:
2619 766 364 354 105 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 739 894 1866 1999 2458 2667 739
Size: 155 972 133 459 209 2355
Fragments arranged by size:
2355 972 459 209 155 133
TatI w'GTAC_w
Cuts at: 255 431 575 3798 255
Size: 176 144 3223 740
Fragments arranged by size:
3223 740 176 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 944 976 3800 3832 944
Size: 32 2824 32 1395
Fragments arranged by size:
2824 1395 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1778 1778
Size: 4283
XmaI C'CCGG_G
Cuts at: 889 1303 889
Size: 414 3869
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BmtI BpmI BsiWI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SrfI SspI StuI SwaI XhoI XmnI ZraI