Restriction map for
pF1KB9857
(Circular) (Six-base) MAPSORT of: pF1KB9857.seq Check: 4955 from: 1 to: 4301
pF1KB9857 4301 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 421 421
Size: 4301
AccI GT'mk_AC
Cuts at: 389 1339 389
Size: 950 3351
Acc65I G'GTAC_C
Cuts at: 1317 1317
Size: 4301
AclI AA'CG_TT
Cuts at: 4141 4141
Size: 4301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 378 2126 2558 3305 3573 378
Size: 1748 432 747 268 1106
Fragments arranged by size:
1748 1106 747 432 268
AflIII A'CryG_T
Cuts at: 1653 2757 3734 1653
Size: 1104 977 2220
Fragments arranged by size:
2220 1104 977
AgeI A'CCGG_T
Cuts at: 2689 2689
Size: 4301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 424 571 424
Size: 147 4154
AlwNI CAG_nnn'CTG
Cuts at: 654 1606 3173 654
Size: 952 1567 1782
Fragments arranged by size:
1782 1567 952
ApaLI G'TGCA_C
Cuts at: 3071 3071
Size: 4301
ApoI r'AATT_y
Cuts at: 1305 3654 3910 1305
Size: 2349 256 1696
Fragments arranged by size:
2349 1696 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4301
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
AvaI C'yCGr_G
Cuts at: 353 811 888 1321 353
Size: 458 77 433 3333
Fragments arranged by size:
3333 458 433 77
BamHI G'GATC_C
Cuts at: 1326 1326
Size: 4301
BanI G'GyrC_C
Cuts at: 408 892 1162 1317 1731 1979 2014 408
Size: 484 270 155 414 248 35 2695
Fragments arranged by size:
2695 484 414 270 248 155 35
BanII G_rGCy'C
Cuts at: 188 295 360 1315 2345 188
Size: 107 65 955 1030 2144
Fragments arranged by size:
2144 1030 955 107 65
BbeI G_GCGC'C
Cuts at: 896 1735 1983 896
Size: 839 248 3214
Fragments arranged by size:
3214 839 248
BbsI GAAGACnn'nnnn_
Cuts at: 1235 1235
Size: 4301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4267
BciVI GTATCCnnnnn_n'
Cuts at: 2199 2960 4301 2199
Size: 761 1341 2199
Fragments arranged by size:
2199 1341 761
BclI T'GATC_A
Cuts at: 113 1821 113
Size: 1708 2593
BglII A'GATC_T
Cuts at: 1816 3853 1816
Size: 2037 2264
BlpI GC'TnA_GC
Cuts at: 622 1413 622
Size: 791 3510
Bme1580I G_kGCm'C
Cuts at: 372 991 1926 2019 3075 372
Size: 619 935 93 1056 1598
Fragments arranged by size:
1598 1056 935 619 93
BmrI ACTGGGnnnn_n'
Cuts at: 1513 1594 1929 4073 1513
Size: 81 335 2144 1741
Fragments arranged by size:
2144 1741 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 344 148
Size: 196 4105
Bpu10I CC'TnA_GC
Cuts at: 737 737
Size: 4301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 655 702 1090 1477 2356 2848 3146 571
Size: 84 47 388 387 879 492 298 1726
Fragments arranged by size:
1726 879 492 388 387 298 84 47
BsaI GGTCTCn'nnnn_
Cuts at: 631 4005 631
Size: 3374 927
BsaAI yAC'GTr
Cuts at: 1654 1654
Size: 4301
BsaBI GATnn'nnATC
Cuts at: 6 244 1815 3852 6
Size: 238 1571 2037 455
Fragments arranged by size:
2037 1571 455 238
BsaHI Gr'CG_yC
Cuts at: 418 493 893 1732 1980 2682 4178 418
Size: 75 400 839 248 702 1496 541
Fragments arranged by size:
1496 839 702 541 400 248 75
BsaWI w'CCGG_w
Cuts at: 1112 1490 2011 2689 2963 3110 3521 1112
Size: 378 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 491 521 491
Size: 30 4271
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 256
Size: 4301
BseYI C'CCAG_C
Cuts at: 473 967 1726 2484 3061 3557 473
Size: 494 759 758 577 496 1217
Fragments arranged by size:
1217 759 758 577 496 494
BsiEI CG_ry'CG
Cuts at: 104 1843 1889 3097 104
Size: 1739 46 1208 1308
Fragments arranged by size:
1739 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 1057 1315 2093 2283 3075 188
Size: 869 258 778 190 792 1414
Fragments arranged by size:
1414 869 792 778 258 190
BsmBI CGTCTCn'nnnn_
Cuts at: 709 709
Size: 4301
Bsp1286I G_dGCh'C
Cuts at: 188 295 360 372 991 1057 1315 1926 2019
Size: 107 65 12 619 66 258 611 93
Cuts at: 2019 2093 2283 2345 3075 188
Size: 74 190 62 730 1414
Fragments arranged by size:
1414 730 619 611 258 190 107 93
74 66 65 62 12
BspEI T'CCGG_A
Cuts at: 1112 3521 1112
Size: 2409 1892
BspHI T'CATG_A
Cuts at: 941 3477 941
Size: 2536 1765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1351 1565 1867 2248 1351
Size: 214 302 381 3404
Fragments arranged by size:
3404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2593 2647 4131 4296 50
Size: 2543 54 1484 165 55
Fragments arranged by size:
2543 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 401 2213 401
Size: 1812 2489
BsrFI r'CCGG_y
Cuts at: 2299 2480 2689 2299
Size: 181 209 3911
Fragments arranged by size:
3911 209 181
BssSI C'ACGA_G
Cuts at: 383 2572 2930 383
Size: 2189 358 1754
Fragments arranged by size:
2189 1754 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1678 1678
Size: 4301
BstBI TT'CG_AA
Cuts at: 2662 2662
Size: 4301
BstEII G'GTnAC_C
Cuts at: 150 150
Size: 4301
BstYI r'GATC_y
Cuts at: 1 239 1326 1793 1816 2151 2397 3409 3495
Size: 238 1087 467 23 335 246 1012 86
Cuts at: 3495 3507 3853 1
Size: 12 346 449
Fragments arranged by size:
1087 1012 467 449 346 335 246 238
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1193 1193
Size: 4301
BtgI C'CryG_G
Cuts at: 1082 1082
Size: 4301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 945 2251 2432 2595 945
Size: 1306 181 163 2651
Fragments arranged by size:
2651 1306 181 163
BtsI GCAGTG_nn'
Cuts at: 1544 1544
Size: 4301
ClaI AT'CG_AT
Cuts at: 1133 1133
Size: 4301
DraI TTT'AAA
Cuts at: 1300 1300
Size: 4301
DrdI GACnn_nn'nnGTC
Cuts at: 2007 2865 2007
Size: 858 3443
EaeI y'GGCC_r
Cuts at: 1005 1886 2060 2451 2478 3823 1005
Size: 881 174 391 27 1345 1483
Fragments arranged by size:
1483 1345 881 391 174 27
EagI C'GGCC_G
Cuts at: 1886 1886
Size: 4301
EarI CTCTTCn'nnn_
Cuts at: 230 746 797 2324 2534 230
Size: 516 51 1527 210 1997
Fragments arranged by size:
1997 1527 516 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 625 2509 2819 2965 4111 625
Size: 1884 310 146 1146 815
Fragments arranged by size:
1884 1146 815 310 146
EcoICRI GAG'CTC
Cuts at: 186 1313 186
Size: 1127 3174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 344 378 2126 2558 3305 3573 148
Size: 196 34 1748 432 747 268 876
Fragments arranged by size:
1748 876 747 432 268 196 34
EcoNI CCTnn'n_nnAGG
Cuts at: 145 271 586 145
Size: 126 315 3860
Fragments arranged by size:
3860 315 126
EcoO109I rG'GnC_Cy
Cuts at: 138 201 349 807 1440 138
Size: 63 148 458 633 2999
Fragments arranged by size:
2999 633 458 148 63
EcoRI G'AATT_C
Cuts at: 1305 1305
Size: 4301
EcoRV GAT'ATC
Cuts at: 318 318
Size: 4301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 715 2320 715
Size: 1605 2696
FspI TGC'GCA
Cuts at: 2082 2082
Size: 4301
HaeII r_GCGC'y
Cuts at: 896 1735 1983 3005 3984 896
Size: 839 248 1022 979 1213
Fragments arranged by size:
1213 1022 979 839 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 215 247 254 286 630 662 687 719 923
Size: 32 7 32 344 32 25 32 204
Cuts at: 923 955 2143 2173 2175 2205 215
Size: 32 1188 30 2 30 2311
Fragments arranged by size:
2311 1188 344 204 32 32 32 32
32 30 30 25 7 2
HincII GTy'rAC
Cuts at: 885 1340 885
Size: 455 3846
KasI G'GCGC_C
Cuts at: 892 1731 1979 892
Size: 839 248 3214
Fragments arranged by size:
3214 839 248
KpnI G_GTAC'C
Cuts at: 1321 1321
Size: 4301
MluI A'CGCG_T
Cuts at: 3734 3734
Size: 4301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 587 2972 3156 587
Size: 2385 184 1732
Fragments arranged by size:
2385 1732 184
MscI TGG'CCA
Cuts at: 2062 2062
Size: 4301
MslI CAynn'nnrTG
Cuts at: 253 406 1087 2290 2417 253
Size: 153 681 1203 127 2137
Fragments arranged by size:
2137 1203 681 153 127
MspA1I CmG'CkG
Cuts at: 469 477 626 1412 1726 2086 3099 3344 469
Size: 8 149 786 314 360 1013 245 1426
Fragments arranged by size:
1426 1013 786 360 314 245 149 8
NaeI GCC'GGC
Cuts at: 2482 2482
Size: 4301
NarI GG'CG_CC
Cuts at: 893 1732 1980 893
Size: 839 248 3214
Fragments arranged by size:
3214 839 248
NcoI C'CATG_G
Cuts at: 1082 1082
Size: 4301
NgoMIV G'CCGG_C
Cuts at: 2480 2480
Size: 4301
NspI r_CATG'y
Cuts at: 555 1297 1354 1854 2761 555
Size: 742 57 500 907 2095
Fragments arranged by size:
2095 907 742 500 57
PasI CC'CwG_GG
Cuts at: 288 288
Size: 4301
PciI A'CATG_T
Cuts at: 2757 2757
Size: 4301
PmeI GTTT'AAAC
Cuts at: 1300 1300
Size: 4301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 4269
PpuMI rG'GwC_Cy
Cuts at: 138 201 349 138
Size: 63 148 4090
Fragments arranged by size:
4090 148 63
PstI C_TGCA'G
Cuts at: 335 1348 2033 335
Size: 1013 685 2603
Fragments arranged by size:
2603 1013 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4301
PvuII CAG'CTG
Cuts at: 1726 2086 1726
Size: 360 3941
RsrII CG'GwC_CG
Cuts at: 2496 2496
Size: 4301
SacI G_AGCT'C
Cuts at: 188 1315 188
Size: 1127 3174
SalI G'TCGA_C
Cuts at: 1338 1338
Size: 4301
SapI GCTCTTCn'nnn_
Cuts at: 2324 2534 2324
Size: 210 4091
SbfI CC_TGCA'GG
Cuts at: 1348 1348
Size: 4301
ScaI AGT'ACT
Cuts at: 3818 3818
Size: 4301
SfcI C'TryA_G
Cuts at: 33 331 1344 2029 3022 3213 33
Size: 298 1013 685 993 191 1121
Fragments arranged by size:
1121 1013 993 685 298 191
SfoI GGC'GCC
Cuts at: 894 1733 1981 894
Size: 839 248 3214
Fragments arranged by size:
3214 839 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4301
SmaI CCC'GGG
Cuts at: 1323 1323
Size: 4301
SmlI C'TyrA_G
Cuts at: 586 634 681 1069 1456 2371 2863 3125 586
Size: 48 47 388 387 915 492 262 1762
Fragments arranged by size:
1762 915 492 388 387 262 48 47
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4301
SphI G_CATG'C
Cuts at: 555 1354 1854 555
Size: 799 500 3002
Fragments arranged by size:
3002 799 500
StuI AGG'CCT
Cuts at: 515 679 1100 515
Size: 164 421 3716
Fragments arranged by size:
3716 421 164
StyI C'CwwG_G
Cuts at: 605 1082 1435 1789 605
Size: 477 353 354 3117
Fragments arranged by size:
3117 477 354 353
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 971 1884 2017 2476 2685 971
Size: 913 133 459 209 2587
Fragments arranged by size:
2587 913 459 209 133
TatI w'GTAC_w
Cuts at: 122 3816 122
Size: 3694 607
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 491 523 562 594 946 978 1212 1244 3818
Size: 32 39 32 352 32 234 32 2574
Cuts at: 3818 3850 491
Size: 32 942
Fragments arranged by size:
2574 942 352 234 39 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2098 2098
Size: 4301
XbaI T'CTAG_A
Cuts at: 1332 1332
Size: 4301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1796 1796
Size: 4301
XmaI C'CCGG_G
Cuts at: 1321 1321
Size: 4301
ZraI GAC'GTC
Cuts at: 419 419
Size: 4301
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI BsgI
BsiWI BsmI BsrGI BssHII BstXI BstZ17I CspCI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI