Restriction map for
pF1KB9858
(Circular) (Six-base) MAPSORT of: pF1KB9858.seq Check: 9529 from: 1 to: 4313
pF1KB9858 4313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2138 2570 3317 3585 1210
Size: 928 432 747 268 1938
Fragments arranged by size:
1938 928 747 432 268
AflIII A'CryG_T
Cuts at: 1665 2769 3746 1665
Size: 1104 977 2232
Fragments arranged by size:
2232 1104 977
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AhdI GACnn_n'nnGTC
Cuts at: 772 772
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 883 1273 1618 3185 883
Size: 390 345 1567 2011
Fragments arranged by size:
2011 1567 390 345
ApaI G_GGCC'C
Cuts at: 969 969
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1333 1333
Size: 4313
BamHI G'GATC_C
Cuts at: 328 484 1338 328
Size: 156 854 3303
Fragments arranged by size:
3303 854 156
BanI G'GyrC_C
Cuts at: 352 595 649 938 1329 1743 1991 2026 352
Size: 243 54 289 391 414 248 35 2639
Fragments arranged by size:
2639 414 391 289 248 243 54 35
BanII G_rGCy'C
Cuts at: 224 232 555 749 969 1090 1171 1283 1327
Size: 8 323 194 220 121 81 112 44
Cuts at: 1327 2357 224
Size: 1030 2180
Fragments arranged by size:
2180 1030 323 220 194 121 112 81
44 8
BbeI G_GCGC'C
Cuts at: 942 1747 1995 942
Size: 805 248 3260
Fragments arranged by size:
3260 805 248
BbvCI CC'TCA_GC
Cuts at: 294 294
Size: 4313
BciVI GTATCCnnnnn_n'
Cuts at: 2211 2972 4313 2211
Size: 761 1341 2211
Fragments arranged by size:
2211 1341 761
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 901 901
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 976 1425 976
Size: 449 3864
Bme1580I G_kGCm'C
Cuts at: 425 839 969 1938 2031 3087 425
Size: 414 130 969 93 1056 1651
Fragments arranged by size:
1651 1056 969 414 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 290 710 971 1525 1606 1941 4085 290
Size: 420 261 554 81 335 2144 518
Fragments arranged by size:
2144 554 518 420 335 261 81
BmtI G_CTAG'C
Cuts at: 575 575
Size: 4313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 519 573 601 655 709 735 763 509
Size: 10 54 28 54 54 26 28 4059
Fragments arranged by size:
4059 54 54 54 28 28 26 10
Bpu10I CC'TnA_GC
Cuts at: 226 294 377 226
Size: 68 83 4162
Fragments arranged by size:
4162 83 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 2860 3158 1489
Size: 879 492 298 2644
Fragments arranged by size:
2644 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 213 577 685 780 4017 213
Size: 364 108 95 3237 509
Fragments arranged by size:
3237 509 364 108 95
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 939 1010 1262 1744 1992 2694 4190 939
Size: 71 252 482 248 702 1496 1062
Fragments arranged by size:
1496 1062 702 482 252 248 71
BsaWI w'CCGG_w
Cuts at: 193 842 1502 2023 2701 2975 3122 3533 193
Size: 649 660 521 678 274 147 411 973
Fragments arranged by size:
973 678 660 649 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 507 537 542 572 1177 1207 507
Size: 30 5 30 605 30 3613
Fragments arranged by size:
3613 605 30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1054 1198 1237 1240 1054
Size: 144 39 3 4127
Fragments arranged by size:
4127 144 39 3
BseYI C'CCAG_C
Cuts at: 1018 1207 1266 1738 2496 3073 3569 1018
Size: 189 59 472 758 577 496 1762
Fragments arranged by size:
1762 758 577 496 472 189 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 4313
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3109 104
Size: 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 169 232 1327 2105 2295 3087 169
Size: 63 1095 778 190 792 1395
Fragments arranged by size:
1395 1095 792 778 190 63
BsmBI CGTCTCn'nnnn_
Cuts at: 1286 1286
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 169 224 232 425 555 749 839 969 1090
Size: 55 8 193 130 194 90 130 121
Cuts at: 1090 1171 1283 1327 1938 2031 2105 2295 2357
Size: 81 112 44 611 93 74 190 62
Cuts at: 2357 3087 169
Size: 730 1395
Fragments arranged by size:
1395 730 611 194 193 190 130 130
121 112 93 90 81 74 62 55
44 8
BspEI T'CCGG_A
Cuts at: 193 842 3533 193
Size: 649 2691 973
Fragments arranged by size:
2691 973 649
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2605 2659 4143 4308 50
Size: 2555 54 1484 165 55
Fragments arranged by size:
2555 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 941 2311 2492 2701 941
Size: 1370 181 209 2553
Fragments arranged by size:
2553 1370 209 181
BsrGI T'GTAC_A
Cuts at: 239 239
Size: 4313
BssSI C'ACGA_G
Cuts at: 863 916 970 2584 2942 863
Size: 53 54 1614 358 2234
Fragments arranged by size:
2234 1614 358 54 53
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 328 484 1338 1805 1828 2163 2409 3421
Size: 327 156 854 467 23 335 246 1012
Cuts at: 3421 3507 3519 3865 1
Size: 86 12 346 449
Fragments arranged by size:
1012 854 467 449 346 335 327 246
156 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 266 1037 266
Size: 771 3542
BtgI C'CryG_G
Cuts at: 107 563 617 671 1061 107
Size: 456 54 54 390 3359
Fragments arranged by size:
3359 456 390 54 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1192 2263 2444 2607 1192
Size: 1071 181 163 2898
Fragments arranged by size:
2898 1071 181 163
BtsI GCAGTG_nn'
Cuts at: 182 1556 182
Size: 1374 2939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1125 1160 1125
Size: 35 4278
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DraIII CAC_nnn'GTG
Cuts at: 495 495
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 110 1238 1898 2072 2463 2490 3835 110
Size: 1128 660 174 391 27 1345 588
Fragments arranged by size:
1345 1128 660 588 391 174 27
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 972 2521 2831 2977 4123 972
Size: 1549 310 146 1146 1162
Fragments arranged by size:
1549 1162 1146 310 146
EcoICRI GAG'CTC
Cuts at: 230 1325 230
Size: 1095 3218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 519 573 601 655 709 735 763 1210
Size: 10 54 28 54 54 26 28 447
Cuts at: 1210 2138 2570 3317 3585 509
Size: 928 432 747 268 1237
Fragments arranged by size:
1237 928 747 447 432 268 54 54
54 28 28 26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 537 537
Size: 4313
EcoO109I rG'GnC_Cy
Cuts at: 965 966 1032 1204 1452 965
Size: 1 66 172 248 3826
Fragments arranged by size:
3826 248 172 66 1
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 942 1747 1995 3017 3996 942
Size: 805 248 1022 979 1259
Fragments arranged by size:
1259 1022 979 805 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 277 309 758 790 844 876 1072 1104 1177
Size: 32 449 32 54 32 196 32 73
Cuts at: 1177 1209 2155 2185 2187 2217 277
Size: 32 946 30 2 30 2373
Fragments arranged by size:
2373 946 449 196 73 54 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1352 1352
Size: 4313
KasI G'GCGC_C
Cuts at: 938 1743 1991 938
Size: 805 248 3260
Fragments arranged by size:
3260 805 248
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 832 2984 3168 832
Size: 2152 184 1977
Fragments arranged by size:
2152 1977 184
MscI TGG'CCA
Cuts at: 112 1240 2074 112
Size: 1128 834 2351
Fragments arranged by size:
2351 1128 834
MslI CAynn'nnrTG
Cuts at: 409 2302 2429 409
Size: 1893 127 2293
Fragments arranged by size:
2293 1893 127
MspA1I CmG'CkG
Cuts at: 755 1424 1738 2098 3111 3356 755
Size: 669 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 669 360 314 245
NaeI GCC'GGC
Cuts at: 943 2494 943
Size: 1551 2762
NarI GG'CG_CC
Cuts at: 939 1744 1992 939
Size: 805 248 3260
Fragments arranged by size:
3260 805 248
NcoI C'CATG_G
Cuts at: 107 617 671 107
Size: 510 54 3749
Fragments arranged by size:
3749 510 54
NgoMIV G'CCGG_C
Cuts at: 941 2492 941
Size: 1551 2762
NheI G'CTAG_C
Cuts at: 571 571
Size: 4313
NspI r_CATG'y
Cuts at: 873 1366 1866 2773 873
Size: 493 500 907 2413
Fragments arranged by size:
2413 907 500 493
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 1032 1032
Size: 4313
PshAI GACnn'nnGTC
Cuts at: 1155 1155
Size: 4313
PspOMI G'GGCC_C
Cuts at: 965 965
Size: 4313
PstI C_TGCA'G
Cuts at: 1360 2045 1360
Size: 685 3628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1738 2098 1738
Size: 360 3953
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 232 1327 232
Size: 1095 3218
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 1356 2041 3034 3225 33
Size: 1323 685 993 191 1121
Fragments arranged by size:
1323 1121 993 685 191
SfoI GGC'GCC
Cuts at: 940 1745 1993 940
Size: 805 248 3260
Fragments arranged by size:
3260 805 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SgrAI Cr'CCGG_yG
Cuts at: 941 941
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1468 2383 2875 3137 1468
Size: 915 492 262 2644
Fragments arranged by size:
2644 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 873 1366 1866 873
Size: 493 500 3320
Fragments arranged by size:
3320 500 493
StyI C'CwwG_G
Cuts at: 107 129 617 671 1447 1801 107
Size: 22 488 54 776 354 2619
Fragments arranged by size:
2619 776 488 354 54 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 201 1896 2029 2488 2697 201
Size: 1695 133 459 209 1817
Fragments arranged by size:
1817 1695 459 209 133
TatI w'GTAC_w
Cuts at: 239 3828 239
Size: 3589 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BsiWI BsmI
BssHII BstEII BstXI BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI