Restriction map for
pF1KB9865
(Circular) (Six-base) MAPSORT of: pF1KB9865.seq Check: 963 from: 1 to: 4409
pF1KB9865 4409 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 218 474 218
Size: 256 4153
AccI GT'mk_AC
Cuts at: 1447 1447
Size: 4409
Acc65I G'GTAC_C
Cuts at: 1425 1425
Size: 4409
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 2666 3413 3681 2234
Size: 432 747 268 2962
Fragments arranged by size:
2962 747 432 268
AfeI AGC'GCT
Cuts at: 304 304
Size: 4409
AflIII A'CryG_T
Cuts at: 317 1761 2865 3842 317
Size: 1444 1104 977 884
Fragments arranged by size:
1444 1104 977 884
AgeI A'CCGG_T
Cuts at: 2797 2797
Size: 4409
AhdI GACnn_n'nnGTC
Cuts at: 294 294
Size: 4409
AleI CACnn'nnGTG
Cuts at: 331 331
Size: 4409
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1186 1218 1186
Size: 32 4377
AlwNI CAG_nnn'CTG
Cuts at: 687 969 1714 3281 687
Size: 282 745 1567 1815
Fragments arranged by size:
1815 1567 745 282
ApaLI G'TGCA_C
Cuts at: 214 487 3179 214
Size: 273 2692 1444
Fragments arranged by size:
2692 1444 273
ApoI r'AATT_y
Cuts at: 1413 3762 4018 1413
Size: 2349 256 1804
Fragments arranged by size:
2349 1804 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 1429 1429
Size: 4409
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 187 230 640 1155 1425 1839 2087 2122 187
Size: 43 410 515 270 414 248 35 2474
Fragments arranged by size:
2474 515 414 410 270 248 43 35
BanII G_rGCy'C
Cuts at: 1094 1423 2453 1094
Size: 329 1030 3050
Fragments arranged by size:
3050 1030 329
BbeI G_GCGC'C
Cuts at: 191 1843 2091 191
Size: 1652 248 2509
Fragments arranged by size:
2509 1652 248
BbsI GAAGACnn'nnnn_
Cuts at: 576 576
Size: 4409
BbvCI CC'TCA_GC
Cuts at: 452 452
Size: 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 225 259 225
Size: 34 4375
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BglI GCCn_nnn'nGGC
Cuts at: 124 649 658 712 1310 124
Size: 525 9 54 598 3223
Fragments arranged by size:
3223 598 525 54 9
BglII A'GATC_T
Cuts at: 1924 3961 1924
Size: 2037 2372
BlpI GC'TnA_GC
Cuts at: 1521 1521
Size: 4409
Bme1580I G_kGCm'C
Cuts at: 218 491 574 681 1160 2034 2127 3183 218
Size: 273 83 107 479 874 93 1056 1444
Fragments arranged by size:
1444 1056 874 479 273 107 93 83
BmrI ACTGGGnnnn_n'
Cuts at: 594 1621 1702 2037 4181 594
Size: 1027 81 335 2144 822
Fragments arranged by size:
2144 1027 822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1176 2776 1176
Size: 1600 2809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 991 1333 1390 463
Size: 528 342 57 3482
Fragments arranged by size:
3482 528 342 57
Bpu10I CC'TnA_GC
Cuts at: 452 1255 452
Size: 803 3606
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 2464 2956 3254 1585
Size: 879 492 298 2740
Fragments arranged by size:
2740 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1280 4113 1280
Size: 2833 1576
BsaAI yAC'GTr
Cuts at: 1762 1762
Size: 4409
BsaBI GATnn'nnATC
Cuts at: 6 856 1923 3960 6
Size: 850 1067 2037 455
Fragments arranged by size:
2037 1067 850 455
BsaHI Gr'CG_yC
Cuts at: 188 1840 2088 2790 4286 188
Size: 1652 248 702 1496 311
Fragments arranged by size:
1652 1496 702 311 248
BsaWI w'CCGG_w
Cuts at: 1598 2119 2797 3071 3218 3629 1598
Size: 521 678 274 147 411 2378
Fragments arranged by size:
2378 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 817 847 1186 1216 817
Size: 30 339 30 4010
Fragments arranged by size:
4010 339 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 580 838 1282 169
Size: 411 258 444 3296
Fragments arranged by size:
3296 444 411 258
BseYI C'CCAG_C
Cuts at: 1834 2592 3169 3665 1834
Size: 758 577 496 2578
Fragments arranged by size:
2578 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 4409
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 218 491 1100 1423 2201 2391 3183 218
Size: 273 609 323 778 190 792 1444
Fragments arranged by size:
1444 792 778 609 323 273 190
BsmBI CGTCTCn'nnnn_
Cuts at: 517 967 517
Size: 450 3959
Bsp1286I G_dGCh'C
Cuts at: 218 491 574 681 1094 1100 1160 1423 2034
Size: 273 83 107 413 6 60 263 611
Cuts at: 2034 2127 2201 2391 2453 3183 218
Size: 93 74 190 62 730 1444
Fragments arranged by size:
1444 730 611 413 273 263 190 107
93 83 74 62 60 6
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 632 3585 632
Size: 2953 1456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 218 474 767 1459 1673 1975 2356 218
Size: 256 293 692 214 302 381 2271
Fragments arranged by size:
2271 692 381 302 293 256 214
BsrBI CCG'CTC
Cuts at: 50 945 2701 2755 4239 4404 50
Size: 895 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 895 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 447 959 2407 2588 2797 447
Size: 512 1448 181 209 2059
Fragments arranged by size:
2059 1448 512 209 181
BssHII G'CGCG_C
Cuts at: 792 794 792
Size: 2 4407
BssSI C'ACGA_G
Cuts at: 2680 3038 2680
Size: 358 4051
BstAPI GCAn_nnn'nTGC
Cuts at: 734 1786 734
Size: 1052 3357
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstEII G'GTnAC_C
Cuts at: 174 436 754 816 1078 174
Size: 262 318 62 262 3505
Fragments arranged by size:
3505 318 262 262 62
BstXI CCAn_nnnn'nTGG
Cuts at: 333 1357 333
Size: 1024 3385
BstYI r'GATC_y
Cuts at: 1 1067 1185 1434 1901 1924 2259 2505 3517
Size: 1066 118 249 467 23 335 246 1012
Cuts at: 3517 3603 3615 3961 1
Size: 86 12 346 449
Fragments arranged by size:
1066 1012 467 449 346 335 249 246
118 86 23 12
BtgI C'CryG_G
Cuts at: 113 707 811 113
Size: 594 104 3711
Fragments arranged by size:
3711 594 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 405 2359 2540 2703 405
Size: 1954 181 163 2111
Fragments arranged by size:
2111 1954 181 163
BtsI GCAGTG_nn'
Cuts at: 1652 1652
Size: 4409
ClaI AT'CG_AT
Cuts at: 860 860
Size: 4409
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DraIII CAC_nnn'GTG
Cuts at: 436 475 777 436
Size: 39 302 4068
Fragments arranged by size:
4068 302 39
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2973 2115
Size: 858 3551
EaeI y'GGCC_r
Cuts at: 116 258 578 650 1058 1296 1994 2168 2559
Size: 142 320 72 408 238 698 174 391
Cuts at: 2559 2586 3931 116
Size: 27 1345 594
Fragments arranged by size:
1345 698 594 408 391 320 238 174
142 72 27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 517 952 1105 2432 2642 517
Size: 435 153 1327 210 2284
Fragments arranged by size:
2284 1327 435 210 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1294 2617 2927 3073 4219 1294
Size: 1323 310 146 1146 1484
Fragments arranged by size:
1484 1323 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1421 1421
Size: 4409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 991 1333 1390 2234 2666 3413 3681 463
Size: 528 342 57 844 432 747 268 1191
Fragments arranged by size:
1191 844 747 528 432 342 268 57
EcoO109I rG'GnC_Cy
Cuts at: 879 966 1365 1548 879
Size: 87 399 183 3740
Fragments arranged by size:
3740 399 183 87
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 763 2190 763
Size: 1427 2982
HaeII r_GCGC'y
Cuts at: 191 306 1843 2091 3113 4092 191
Size: 115 1537 248 1022 979 508
Fragments arranged by size:
1537 1022 979 508 248 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 2251 2281 2283 2313 280
Size: 32 1939 30 2 30 2376
Fragments arranged by size:
2376 1939 32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
KasI G'GCGC_C
Cuts at: 187 1839 2087 187
Size: 1652 248 2509
Fragments arranged by size:
2509 1652 248
KpnI G_GTAC'C
Cuts at: 1429 1429
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3080 3264 3080
Size: 184 4225
MscI TGG'CCA
Cuts at: 260 652 1060 2170 260
Size: 392 408 1110 2499
Fragments arranged by size:
2499 1110 408 392
MslI CAynn'nnrTG
Cuts at: 331 424 592 862 1027 2398 2525 331
Size: 93 168 270 165 1371 127 2215
Fragments arranged by size:
2215 1371 270 168 165 127 93
MspA1I CmG'CkG
Cuts at: 115 344 813 843 1172 1377 1520 1834 2194
Size: 229 469 30 329 205 143 314 360
Cuts at: 2194 3207 3452 115
Size: 1013 245 1072
Fragments arranged by size:
1072 1013 469 360 329 314 245 229
205 143 30
NaeI GCC'GGC
Cuts at: 449 961 2590 449
Size: 512 1629 2268
Fragments arranged by size:
2268 1629 512
NarI GG'CG_CC
Cuts at: 188 1840 2088 188
Size: 1652 248 2509
Fragments arranged by size:
2509 1652 248
NgoMIV G'CCGG_C
Cuts at: 447 959 2588 447
Size: 512 1629 2268
Fragments arranged by size:
2268 1629 512
NspI r_CATG'y
Cuts at: 321 357 1462 1962 2869 321
Size: 36 1105 500 907 1861
Fragments arranged by size:
1861 1105 907 500 36
PasI CC'CwG_GG
Cuts at: 1214 1214
Size: 4409
PciI A'CATG_T
Cuts at: 317 2865 317
Size: 2548 1861
PflMI CCAn_nnn'nTGG
Cuts at: 777 777
Size: 4409
PfoI T'CCnGG_A
Cuts at: 1001 1367 1001
Size: 366 4043
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1186 1218 3548 3580 1186
Size: 32 2330 32 2015
Fragments arranged by size:
2330 2015 32 32
PpuMI rG'GwC_Cy
Cuts at: 966 1365 966
Size: 399 4010
PshAI GACnn'nnGTC
Cuts at: 1392 1392
Size: 4409
PstI C_TGCA'G
Cuts at: 1177 1456 2141 1177
Size: 279 685 3445
Fragments arranged by size:
3445 685 279
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 1834 2194 1834
Size: 360 4049
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 1423 1423
Size: 4409
SacII CC_GC'GG
Cuts at: 116 814 116
Size: 698 3711
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 952 1105 2432 2642 952
Size: 153 1327 210 2719
Fragments arranged by size:
2719 1327 210 153
SbfI CC_TGCA'GG
Cuts at: 1456 1456
Size: 4409
ScaI AGT'ACT
Cuts at: 3926 3926
Size: 4409
SexAI A'CCwGG_T
Cuts at: 170 170
Size: 4409
SfcI C'TryA_G
Cuts at: 33 135 1173 1383 1452 2137 3130 3321 33
Size: 102 1038 210 69 685 993 191 1121
Fragments arranged by size:
1121 1038 993 685 210 191 102 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 124 124
Size: 4409
SfoI GGC'GCC
Cuts at: 189 1841 2089 189
Size: 1652 248 2509
Fragments arranged by size:
2509 1652 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 1431 1431
Size: 4409
SmlI C'TyrA_G
Cuts at: 1564 2479 2971 3233 1564
Size: 915 492 262 2740
Fragments arranged by size:
2740 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3909
StuI AGG'CCT
Cuts at: 1313 1313
Size: 4409
StyI C'CwwG_G
Cuts at: 1543 1897 1543
Size: 354 4055
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1051 1992 2125 2584 2793 1051
Size: 941 133 459 209 2667
Fragments arranged by size:
2667 941 459 209 133
TatI w'GTAC_w
Cuts at: 833 1115 3924 833
Size: 282 2809 1318
Fragments arranged by size:
2809 1318 282
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4377
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1904 1904
Size: 4409
XmaI C'CCGG_G
Cuts at: 1429 1429
Size: 4409
XmnI GAAnn'nnTTC
Cuts at: 1201 1201
Size: 4409
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AlfI ApaI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BsmI BsrGI BstZ17I Bsu36I CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
PspOMI PspXI PsrI SanDI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI