Restriction map for
pF1KB9866
(Circular) (Six-base) MAPSORT of: pF1KB9866.seq Check: 2732 from: 1 to: 4418
pF1KB9866 4418 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 274 1076 274
Size: 802 3616
AccI GT'mk_AC
Cuts at: 1456 1456
Size: 4418
Acc65I G'GTAC_C
Cuts at: 1434 1434
Size: 4418
AclI AA'CG_TT
Cuts at: 4258 4258
Size: 4418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 652 2243 2675 3422 3690 472
Size: 180 1591 432 747 268 1200
Fragments arranged by size:
1591 1200 747 432 268 180
AflIII A'CryG_T
Cuts at: 236 1770 2874 3851 236
Size: 1534 1104 977 803
Fragments arranged by size:
1534 1104 977 803
AgeI A'CCGG_T
Cuts at: 2806 2806
Size: 4418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 229 950 229
Size: 721 3697
AlwNI CAG_nnn'CTG
Cuts at: 1723 3290 1723
Size: 1567 2851
ApaI G_GGCC'C
Cuts at: 978 978
Size: 4418
ApaLI G'TGCA_C
Cuts at: 3188 3188
Size: 4418
ApoI r'AATT_y
Cuts at: 1422 3771 4027 1422
Size: 2349 256 1813
Fragments arranged by size:
2349 1813 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 970 1279 1438 970
Size: 309 159 3950
Fragments arranged by size:
3950 309 159
BamHI G'GATC_C
Cuts at: 1443 1443
Size: 4418
BanI G'GyrC_C
Cuts at: 1218 1434 1848 2096 2131 1218
Size: 216 414 248 35 3505
Fragments arranged by size:
3505 414 248 216 35
BanII G_rGCy'C
Cuts at: 466 978 1432 2462 466
Size: 512 454 1030 2422
Fragments arranged by size:
2422 1030 512 454
BbeI G_GCGC'C
Cuts at: 1222 1852 2100 1222
Size: 630 248 3540
Fragments arranged by size:
3540 630 248
BbsI GAAGACnn'nnnn_
Cuts at: 138 549 712 138
Size: 411 163 3844
Fragments arranged by size:
3844 411 163
BbvCI CC'TCA_GC
Cuts at: 1205 1205
Size: 4418
BciVI GTATCCnnnnn_n'
Cuts at: 2316 3077 4418 2316
Size: 761 1341 2316
Fragments arranged by size:
2316 1341 761
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BglI GCCn_nnn'nGGC
Cuts at: 821 838 1099 1172 1363 1372 821
Size: 17 261 73 191 9 3867
Fragments arranged by size:
3867 261 191 73 17 9
BglII A'GATC_T
Cuts at: 770 1933 3970 770
Size: 1163 2037 1218
Fragments arranged by size:
2037 1218 1163
BlpI GC'TnA_GC
Cuts at: 1530 1530
Size: 4418
Bme1580I G_kGCm'C
Cuts at: 978 2043 2136 3192 978
Size: 1065 93 1056 2204
Fragments arranged by size:
2204 1065 1056 93
BmgBI CAC'GTC
Cuts at: 237 237
Size: 4418
BmrI ACTGGGnnnn_n'
Cuts at: 1098 1630 1711 2046 4190 1098
Size: 532 81 335 2144 1326
Fragments arranged by size:
2144 1326 532 335 81
BmtI G_CTAG'C
Cuts at: 1009 1009
Size: 4418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 675 787 1273 138
Size: 537 112 486 3283
Fragments arranged by size:
3283 537 486 112
Bpu10I CC'TnA_GC
Cuts at: 1205 1205
Size: 4418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1111 1594 2473 2965 3263 781
Size: 330 483 879 492 298 1936
Fragments arranged by size:
1936 879 492 483 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 1337 4122 1337
Size: 2785 1633
BsaAI yAC'GTr
Cuts at: 393 1771 393
Size: 1378 3040
BsaBI GATnn'nnATC
Cuts at: 6 1932 3969 6
Size: 1926 2037 455
Fragments arranged by size:
2037 1926 455
BsaHI Gr'CG_yC
Cuts at: 830 1219 1849 2097 2799 4295 830
Size: 389 630 248 702 1496 953
Fragments arranged by size:
1496 953 702 630 389 248
BsaWI w'CCGG_w
Cuts at: 1395 1607 2128 2806 3080 3227 3638 1395
Size: 212 521 678 274 147 411 2175
Fragments arranged by size:
2175 678 521 411 274 212 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 223
Size: 30 4388
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 454 457 1297 1300 1303 451
Size: 3 3 840 3 3 3566
Fragments arranged by size:
3566 840 3 3 3 3
BseYI C'CCAG_C
Cuts at: 216 275 466 846 877 1162 1843 2601 3178
Size: 59 191 380 31 285 681 758 577
Cuts at: 3178 3674 216
Size: 496 960
Fragments arranged by size:
960 758 681 577 496 380 285 191
59 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 382
Size: 4418
BsiEI CG_ry'CG
Cuts at: 104 513 654 889 1106 1960 2006 3214 104
Size: 409 141 235 217 854 46 1208 1308
Fragments arranged by size:
1308 1208 854 409 235 217 141 46
BsiHKAI G_wGCw'C
Cuts at: 158 1432 2210 2400 3192 158
Size: 1274 778 190 792 1384
Fragments arranged by size:
1384 1274 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 158 466 978 1432 2043 2136 2210 2400 2462
Size: 308 512 454 611 93 74 190 62
Cuts at: 2462 3192 158
Size: 730 1384
Fragments arranged by size:
1384 730 611 512 454 308 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3638 3638
Size: 4418
BspHI T'CATG_A
Cuts at: 3594 3594
Size: 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 274 1076 1468 1682 1984 2365 274
Size: 802 392 214 302 381 2327
Fragments arranged by size:
2327 802 392 381 302 214
BsrBI CCG'CTC
Cuts at: 50 639 1110 1241 2710 2764 4248 4413 50
Size: 589 471 131 1469 54 1484 165 55
Fragments arranged by size:
1484 1469 589 471 165 131 55 54
BsrDI GCAATG_nn'
Cuts at: 2330 2330
Size: 4418
BsrFI r'CCGG_y
Cuts at: 601 2416 2597 2806 601
Size: 1815 181 209 2213
Fragments arranged by size:
2213 1815 209 181
BssHII G'CGCG_C
Cuts at: 1021 1021
Size: 4418
BssSI C'ACGA_G
Cuts at: 1198 2689 3047 1198
Size: 1491 358 2569
Fragments arranged by size:
2569 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 405 1795 405
Size: 1390 3028
BstBI TT'CG_AA
Cuts at: 2779 2779
Size: 4418
BstXI CCAn_nnnn'nTGG
Cuts at: 675 675
Size: 4418
BstYI r'GATC_y
Cuts at: 1 770 1443 1910 1933 2268 2514 3526 3612
Size: 769 673 467 23 335 246 1012 86
Cuts at: 3612 3624 3970 1
Size: 12 346 449
Fragments arranged by size:
1012 769 673 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 161 224 513 698 959 989 1307 107
Size: 54 63 289 185 261 30 318 3218
Fragments arranged by size:
3218 318 289 261 185 63 54 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 260 582 2368 2549 2712 260
Size: 322 1786 181 163 1966
Fragments arranged by size:
1966 1786 322 181 163
BtsI GCAGTG_nn'
Cuts at: 1661 1661
Size: 4418
DraI TTT'AAA
Cuts at: 1417 1417
Size: 4418
DraIII CAC_nnn'GTG
Cuts at: 405 405
Size: 4418
DrdI GACnn_nn'nnGTC
Cuts at: 2124 2982 2124
Size: 858 3560
EaeI y'GGCC_r
Cuts at: 530 603 886 1056 1103 1406 2003 2177 2568
Size: 73 283 170 47 303 597 174 391
Cuts at: 2568 2595 3940 530
Size: 27 1345 1008
Fragments arranged by size:
1345 1008 597 391 303 283 174 170
73 47 27
EagI C'GGCC_G
Cuts at: 886 1103 2003 886
Size: 217 900 3301
Fragments arranged by size:
3301 900 217
EarI CTCTTCn'nnn_
Cuts at: 410 448 2441 2651 410
Size: 38 1993 210 2177
Fragments arranged by size:
2177 1993 210 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 244 1029 2626 2936 3082 4228 244
Size: 785 1597 310 146 1146 434
Fragments arranged by size:
1597 1146 785 434 310 146
EcoICRI GAG'CTC
Cuts at: 1430 1430
Size: 4418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 138 472 652 675 787 1273 2243 2675 3422
Size: 334 180 23 112 486 970 432 747
Cuts at: 3422 3690 138
Size: 268 866
Fragments arranged by size:
970 866 747 486 432 334 268 180
112 23
EcoNI CCTnn'n_nnAGG
Cuts at: 946 1015 946
Size: 69 4349
EcoO109I rG'GnC_Cy
Cuts at: 311 840 974 975 1274 1557 311
Size: 529 134 1 299 283 3172
Fragments arranged by size:
3172 529 299 283 134 1
EcoRI G'AATT_C
Cuts at: 1422 1422
Size: 4418
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 729 2437 729
Size: 1708 2710
FspI TGC'GCA
Cuts at: 409 526 926 2199 409
Size: 117 400 1273 2628
Fragments arranged by size:
2628 1273 400 117
FspAI rTGC'GCAy
Cuts at: 409 526 926 409
Size: 117 400 3901
Fragments arranged by size:
3901 400 117
HaeII r_GCGC'y
Cuts at: 346 541 1222 1265 1852 2100 3122 4101 346
Size: 195 681 43 587 248 1022 979 663
Fragments arranged by size:
1022 979 681 663 587 248 195 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 481 513 843 875 2260 2290 2292
Size: 32 211 32 330 32 1385 30 2
Cuts at: 2292 2322 238
Size: 30 2334
Fragments arranged by size:
2334 1385 330 211 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1457 1457
Size: 4418
KasI G'GCGC_C
Cuts at: 1218 1848 2096 1218
Size: 630 248 3540
Fragments arranged by size:
3540 630 248
KpnI G_GTAC'C
Cuts at: 1438 1438
Size: 4418
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3089 3273 3089
Size: 184 4234
MscI TGG'CCA
Cuts at: 1408 2179 1408
Size: 771 3647
MslI CAynn'nnrTG
Cuts at: 211 358 724 2407 2534 211
Size: 147 366 1683 127 2095
Fragments arranged by size:
2095 1683 366 147 127
MspA1I CmG'CkG
Cuts at: 226 450 515 647 656 846 934 991 1309
Size: 224 65 132 9 190 88 57 318
Cuts at: 1309 1382 1529 1843 2203 3216 3461 226
Size: 73 147 314 360 1013 245 1183
Fragments arranged by size:
1183 1013 360 318 314 245 224 190
147 132 88 73 65 57 9
NaeI GCC'GGC
Cuts at: 603 2599 603
Size: 1996 2422
NarI GG'CG_CC
Cuts at: 1219 1849 2097 1219
Size: 630 248 3540
Fragments arranged by size:
3540 630 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4418
NgoMIV G'CCGG_C
Cuts at: 601 2597 601
Size: 1996 2422
NheI G'CTAG_C
Cuts at: 1005 1005
Size: 4418
NspI r_CATG'y
Cuts at: 765 1471 1971 2878 765
Size: 706 500 907 2305
Fragments arranged by size:
2305 907 706 500
PasI CC'CwG_GG
Cuts at: 942 942
Size: 4418
PciI A'CATG_T
Cuts at: 2874 2874
Size: 4418
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PmlI CAC'GTG
Cuts at: 393 393
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4386
PpuMI rG'GwC_Cy
Cuts at: 1274 1274
Size: 4418
PshAI GACnn'nnGTC
Cuts at: 553 1349 553
Size: 796 3622
PspOMI G'GGCC_C
Cuts at: 974 974
Size: 4418
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 286 318 286
Size: 32 4386
PstI C_TGCA'G
Cuts at: 350 1158 1465 2150 350
Size: 808 307 685 2618
Fragments arranged by size:
2618 808 685 307
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 450 1843 2203 450
Size: 1393 360 2665
Fragments arranged by size:
2665 1393 360
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 1432 1432
Size: 4418
SacII CC_GC'GG
Cuts at: 227 516 992 1310 227
Size: 289 476 318 3335
Fragments arranged by size:
3335 476 318 289
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SanDI GG'GwC_CC
Cuts at: 1274 1274
Size: 4418
SapI GCTCTTCn'nnn_
Cuts at: 2441 2651 2441
Size: 210 4208
SbfI CC_TGCA'GG
Cuts at: 1465 1465
Size: 4418
ScaI AGT'ACT
Cuts at: 610 3935 610
Size: 3325 1093
SfcI C'TryA_G
Cuts at: 33 346 1154 1461 2146 3139 3330 33
Size: 313 808 307 685 993 191 1121
Fragments arranged by size:
1121 993 808 685 313 307 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1363 1372 1363
Size: 9 4409
SfoI GGC'GCC
Cuts at: 1220 1850 2098 1220
Size: 630 248 3540
Fragments arranged by size:
3540 630 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SmaI CCC'GGG
Cuts at: 972 1440 972
Size: 468 3950
SmlI C'TyrA_G
Cuts at: 796 1126 1573 2488 2980 3242 796
Size: 330 447 915 492 262 1972
Fragments arranged by size:
1972 915 492 447 330 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 1471 1971 1471
Size: 500 3918
StyI C'CwwG_G
Cuts at: 107 473 1552 1906 107
Size: 366 1079 354 2619
Fragments arranged by size:
2619 1079 366 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 289 1030 2001 2134 2593 2802 289
Size: 741 971 133 459 209 1905
Fragments arranged by size:
1905 971 741 459 209 133
TatI w'GTAC_w
Cuts at: 608 3933 608
Size: 3325 1093
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 223 255 3935 3967 223
Size: 32 3680 32 674
Fragments arranged by size:
3680 674 32 32
Tth111I GACn'n_nGTC
Cuts at: 2215 2215
Size: 4418
XbaI T'CTAG_A
Cuts at: 1449 1449
Size: 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1913 1913
Size: 4418
XmaI C'CCGG_G
Cuts at: 970 1438 970
Size: 468 3950
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI HindIII HpaI
MfeI NdeI NotI NruI NsiI PacI PflMI PfoI
PsiI PspXI SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI