Restriction map for
pF1KB9867
(Circular) (Six-base) MAPSORT of: pF1KB9867.seq Check: 9943 from: 1 to: 4451
pF1KB9867 4451 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 275 275
Size: 4451
AatII G_ACGT'C
Cuts at: 1397 1397
Size: 4451
AccI GT'mk_AC
Cuts at: 1489 1489
Size: 4451
Acc65I G'GTAC_C
Cuts at: 1467 1467
Size: 4451
AclI AA'CG_TT
Cuts at: 4291 4291
Size: 4451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 2276 2708 3455 3723 940
Size: 1336 432 747 268 1668
Fragments arranged by size:
1668 1336 747 432 268
AflIII A'CryG_T
Cuts at: 1049 1803 2907 3884 1049
Size: 754 1104 977 1616
Fragments arranged by size:
1616 1104 977 754
AgeI A'CCGG_T
Cuts at: 2839 2839
Size: 4451
AlwNI CAG_nnn'CTG
Cuts at: 358 1756 3323 358
Size: 1398 1567 1486
Fragments arranged by size:
1567 1486 1398
ApaLI G'TGCA_C
Cuts at: 3221 3221
Size: 4451
ApoI r'AATT_y
Cuts at: 1455 3804 4060 1455
Size: 2349 256 1846
Fragments arranged by size:
2349 1846 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4451
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
AvaI C'yCGr_G
Cuts at: 1471 1471
Size: 4451
BamHI G'GATC_C
Cuts at: 1476 1476
Size: 4451
BanI G'GyrC_C
Cuts at: 1467 1881 2129 2164 1467
Size: 414 248 35 3754
Fragments arranged by size:
3754 414 248 35
BanII G_rGCy'C
Cuts at: 1297 1465 2495 1297
Size: 168 1030 3253
Fragments arranged by size:
3253 1030 168
BbeI G_GCGC'C
Cuts at: 1885 2133 1885
Size: 248 4203
BbsI GAAGACnn'nnnn_
Cuts at: 928 928
Size: 4451
BbvCI CC'TCA_GC
Cuts at: 676 676
Size: 4451
BciVI GTATCCnnnnn_n'
Cuts at: 187 2349 3110 4451 187
Size: 2162 761 1341 187
Fragments arranged by size:
2162 1341 761 187
BclI T'GATC_A
Cuts at: 455 536 935 1971 455
Size: 81 399 1036 2935
Fragments arranged by size:
2935 1036 399 81
BglII A'GATC_T
Cuts at: 315 1966 4003 315
Size: 1651 2037 763
Fragments arranged by size:
2037 1651 763
BlpI GC'TnA_GC
Cuts at: 589 1563 589
Size: 974 3477
Bme1580I G_kGCm'C
Cuts at: 2076 2169 3225 2076
Size: 93 1056 3302
Fragments arranged by size:
3302 1056 93
BmgBI CAC'GTC
Cuts at: 1050 1050
Size: 4451
BmrI ACTGGGnnnn_n'
Cuts at: 152 574 1663 1744 2079 4223 152
Size: 422 1089 81 335 2144 380
Fragments arranged by size:
2144 1089 422 380 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2818 2818
Size: 4451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 654 1423 468
Size: 186 769 3496
Fragments arranged by size:
3496 769 186
Bpu10I CC'TnA_GC
Cuts at: 676 676
Size: 4451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1627 2506 2998 3296 1627
Size: 879 492 298 2782
Fragments arranged by size:
2782 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 4155 643
Size: 3512 939
BsaAI yAC'GTr
Cuts at: 1804 1804
Size: 4451
BsaBI GATnn'nnATC
Cuts at: 6 1965 4002 6
Size: 1959 2037 455
Fragments arranged by size:
2037 1959 455
BsaHI Gr'CG_yC
Cuts at: 1394 1882 2130 2832 4328 1394
Size: 488 248 702 1496 1517
Fragments arranged by size:
1517 1496 702 488 248
BsaWI w'CCGG_w
Cuts at: 494 873 1640 2161 2839 3113 3260 3671 494
Size: 379 767 521 678 274 147 411 1274
Fragments arranged by size:
1274 767 678 521 411 379 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 652 682 1339 1369 652
Size: 30 657 30 3734
Fragments arranged by size:
3734 657 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1287 1333 1287
Size: 46 4405
BseYI C'CCAG_C
Cuts at: 1359 1876 2634 3211 3707 1359
Size: 517 758 577 496 2103
Fragments arranged by size:
2103 758 577 517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 604
Size: 4451
BsiEI CG_ry'CG
Cuts at: 104 1993 2039 3247 104
Size: 1889 46 1208 1308
Fragments arranged by size:
1889 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 427 1465 2243 2433 3225 427
Size: 1038 778 190 792 1653
Fragments arranged by size:
1653 1038 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1006 1177 1401 1006
Size: 171 224 4056
Fragments arranged by size:
4056 224 171
Bsp1286I G_dGCh'C
Cuts at: 427 1297 1465 2076 2169 2243 2433 2495 3225
Size: 870 168 611 93 74 190 62 730
Cuts at: 3225 427
Size: 1653
Fragments arranged by size:
1653 870 730 611 190 168 93 74
62
BspEI T'CCGG_A
Cuts at: 3671 3671
Size: 4451
BspHI T'CATG_A
Cuts at: 734 1230 3627 734
Size: 496 2397 1558
Fragments arranged by size:
2397 1558 496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 1501 1715 2017 2398 275
Size: 1226 214 302 381 2328
Fragments arranged by size:
2328 1226 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2743 2797 4281 4446 50
Size: 2693 54 1484 165 55
Fragments arranged by size:
2693 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 788 2363 788
Size: 1575 2876
BsrFI r'CCGG_y
Cuts at: 2449 2630 2839 2449
Size: 181 209 4061
Fragments arranged by size:
4061 209 181
BssSI C'ACGA_G
Cuts at: 1238 2722 3080 1238
Size: 1484 358 2609
Fragments arranged by size:
2609 1484 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1828 1828
Size: 4451
BstBI TT'CG_AA
Cuts at: 2812 2812
Size: 4451
BstEII G'GTnAC_C
Cuts at: 279 279
Size: 4451
BstXI CCAn_nnnn'nTGG
Cuts at: 222 642 1344 222
Size: 420 702 3329
Fragments arranged by size:
3329 702 420
BstYI r'GATC_y
Cuts at: 1 315 755 1409 1476 1943 1966 2301 2547
Size: 314 440 654 67 467 23 335 246
Cuts at: 2547 3559 3645 3657 4003 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 654 467 449 440 346 335 314
246 86 67 23 12
BtgI C'CryG_G
Cuts at: 131 131
Size: 4451
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1228 1317 2401 2582 2745 1228
Size: 89 1084 181 163 2934
Fragments arranged by size:
2934 1084 181 163 89
BtsI GCAGTG_nn'
Cuts at: 182 359 428 1694 182
Size: 177 69 1266 2939
Fragments arranged by size:
2939 1266 177 69
DraI TTT'AAA
Cuts at: 1450 1450
Size: 4451
DrdI GACnn_nn'nnGTC
Cuts at: 1393 2157 3015 1393
Size: 764 858 2829
Fragments arranged by size:
2829 858 764
EaeI y'GGCC_r
Cuts at: 326 728 2036 2210 2601 2628 3973 326
Size: 402 1308 174 391 27 1345 804
Fragments arranged by size:
1345 1308 804 402 391 174 27
EagI C'GGCC_G
Cuts at: 2036 2036
Size: 4451
EarI CTCTTCn'nnn_
Cuts at: 2474 2684 2474
Size: 210 4241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2659 2969 3115 4261 2659
Size: 310 146 1146 2849
Fragments arranged by size:
2849 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1463 1463
Size: 4451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 468 654 940 1423 2276 2708 3455 3723 468
Size: 186 286 483 853 432 747 268 1196
Fragments arranged by size:
1196 853 747 483 432 286 268 186
EcoNI CCTnn'n_nnAGG
Cuts at: 750 801 1312 750
Size: 51 511 3889
Fragments arranged by size:
3889 511 51
EcoO109I rG'GnC_Cy
Cuts at: 386 1305 1590 386
Size: 919 285 3247
Fragments arranged by size:
3247 919 285
EcoRI G'AATT_C
Cuts at: 1455 1455
Size: 4451
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 543 912 2470 543
Size: 369 1558 2524
Fragments arranged by size:
2524 1558 369
FspI TGC'GCA
Cuts at: 2232 2232
Size: 4451
HaeII r_GCGC'y
Cuts at: 1885 2133 3155 4134 1885
Size: 248 1022 979 2202
Fragments arranged by size:
2202 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1232 1264 1339 1371 2293 2323 2325 2355 1232
Size: 32 75 32 922 30 2 30 3328
Fragments arranged by size:
3328 922 75 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1490 1490
Size: 4451
HindIII A'AGCT_T
Cuts at: 711 711
Size: 4451
KasI G'GCGC_C
Cuts at: 1881 2129 1881
Size: 248 4203
KpnI G_GTAC'C
Cuts at: 1471 1471
Size: 4451
MluI A'CGCG_T
Cuts at: 3884 3884
Size: 4451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 285 1061 1437 3122 3306 285
Size: 776 376 1685 184 1430
Fragments arranged by size:
1685 1430 776 376 184
MscI TGG'CCA
Cuts at: 730 2212 730
Size: 1482 2969
MslI CAynn'nnrTG
Cuts at: 403 580 724 943 2440 2567 403
Size: 177 144 219 1497 127 2287
Fragments arranged by size:
2287 1497 219 177 144 127
MspA1I CmG'CkG
Cuts at: 593 1327 1562 1876 2236 3249 3494 593
Size: 734 235 314 360 1013 245 1550
Fragments arranged by size:
1550 1013 734 360 314 245 235
NaeI GCC'GGC
Cuts at: 2632 2632
Size: 4451
NarI GG'CG_CC
Cuts at: 1882 2130 1882
Size: 248 4203
NcoI C'CATG_G
Cuts at: 131 131
Size: 4451
NdeI CA'TA_TG
Cuts at: 687 687
Size: 4451
NgoMIV G'CCGG_C
Cuts at: 2630 2630
Size: 4451
NspI r_CATG'y
Cuts at: 1288 1504 2004 2911 1288
Size: 216 500 907 2828
Fragments arranged by size:
2828 907 500 216
PciI A'CATG_T
Cuts at: 2907 2907
Size: 4451
PmeI GTTT'AAAC
Cuts at: 1450 1450
Size: 4451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4419
PpuMI rG'GwC_Cy
Cuts at: 386 1305 386
Size: 919 3532
PstI C_TGCA'G
Cuts at: 1498 2183 1498
Size: 685 3766
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4451
PvuII CAG'CTG
Cuts at: 593 1327 1876 2236 593
Size: 734 549 360 2808
Fragments arranged by size:
2808 734 549 360
RsrII CG'GwC_CG
Cuts at: 2646 2646
Size: 4451
SacI G_AGCT'C
Cuts at: 1465 1465
Size: 4451
SalI G'TCGA_C
Cuts at: 1488 1488
Size: 4451
SapI GCTCTTCn'nnn_
Cuts at: 2474 2684 2474
Size: 210 4241
SbfI CC_TGCA'GG
Cuts at: 1498 1498
Size: 4451
ScaI AGT'ACT
Cuts at: 3968 3968
Size: 4451
SfcI C'TryA_G
Cuts at: 33 759 1149 1494 2179 3172 3363 33
Size: 726 390 345 685 993 191 1121
Fragments arranged by size:
1121 993 726 685 390 345 191
SfoI GGC'GCC
Cuts at: 1883 2131 1883
Size: 248 4203
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4451
SmaI CCC'GGG
Cuts at: 1473 1473
Size: 4451
SmlI C'TyrA_G
Cuts at: 1606 2521 3013 3275 1606
Size: 915 492 262 2782
Fragments arranged by size:
2782 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4451
SphI G_CATG'C
Cuts at: 1504 2004 1504
Size: 500 3951
StyI C'CwwG_G
Cuts at: 131 275 302 518 797 1253 1343 1585 1939
Size: 144 27 216 279 456 90 242 354
Cuts at: 1939 131
Size: 2643
Fragments arranged by size:
2643 456 354 279 242 216 144 90
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 393 931 1055 1359 1454 2034 2167 2626 2835
Size: 538 124 304 95 580 133 459 209
Cuts at: 2835 393
Size: 2009
Fragments arranged by size:
2009 580 538 459 304 209 133 124
95
TatI w'GTAC_w
Cuts at: 561 3966 561
Size: 3405 1046
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 256 288 3968 4000 256
Size: 32 3680 32 707
Fragments arranged by size:
3680 707 32 32
Tth111I GACn'n_nGTC
Cuts at: 555 927 2248 555
Size: 372 1321 2758
Fragments arranged by size:
2758 1321 372
XbaI T'CTAG_A
Cuts at: 1482 1482
Size: 4451
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1946 1946
Size: 4451
XmaI C'CCGG_G
Cuts at: 1471 1471
Size: 4451
ZraI GAC'GTC
Cuts at: 1395 1395
Size: 4451
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI