Restriction map for
pF1KB9869
(Circular) (Six-base) MAPSORT of: pF1KB9869.seq Check: 4356 from: 1 to: 4493
pF1KB9869 4493 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AatII G_ACGT'C
Cuts at: 1175 1175
Size: 4493
AccI GT'mk_AC
Cuts at: 749 1531 749
Size: 782 3711
Acc65I G'GTAC_C
Cuts at: 1509 1509
Size: 4493
AclI AA'CG_TT
Cuts at: 4333 4333
Size: 4493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 199 2318 2750 3497 3765 164
Size: 35 2119 432 747 268 892
Fragments arranged by size:
2119 892 747 432 268 35
AflIII A'CryG_T
Cuts at: 1845 2949 3926 1845
Size: 1104 977 2412
Fragments arranged by size:
2412 1104 977
AgeI A'CCGG_T
Cuts at: 2881 2881
Size: 4493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 582 949 582
Size: 367 4126
AlwNI CAG_nnn'CTG
Cuts at: 189 601 1069 1798 3365 189
Size: 412 468 729 1567 1317
Fragments arranged by size:
1567 1317 729 468 412
ApaI G_GGCC'C
Cuts at: 913 913
Size: 4493
ApaLI G'TGCA_C
Cuts at: 328 3263 328
Size: 2935 1558
ApoI r'AATT_y
Cuts at: 171 1497 3846 4102 171
Size: 1326 2349 256 562
Fragments arranged by size:
2349 1326 562 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4493
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
AvaI C'yCGr_G
Cuts at: 116 275 316 1513 116
Size: 159 41 1197 3096
Fragments arranged by size:
3096 1197 159 41
BamHI G'GATC_C
Cuts at: 1518 1518
Size: 4493
BanI G'GyrC_C
Cuts at: 1509 1923 2171 2206 1509
Size: 414 248 35 3796
Fragments arranged by size:
3796 414 248 35
BanII G_rGCy'C
Cuts at: 386 913 1102 1507 2537 386
Size: 527 189 405 1030 2342
Fragments arranged by size:
2342 1030 527 405 189
BbeI G_GCGC'C
Cuts at: 1927 2175 1927
Size: 248 4245
BbvCI CC'TCA_GC
Cuts at: 1062 1062
Size: 4493
BciVI GTATCCnnnnn_n'
Cuts at: 2391 3152 4493 2391
Size: 761 1341 2391
Fragments arranged by size:
2391 1341 761
BclI T'GATC_A
Cuts at: 2013 2013
Size: 4493
BglI GCCn_nnn'nGGC
Cuts at: 231 434 526 231
Size: 203 92 4198
Fragments arranged by size:
4198 203 92
BglII A'GATC_T
Cuts at: 2008 4045 2008
Size: 2037 2456
BlpI GC'TnA_GC
Cuts at: 209 1605 209
Size: 1396 3097
Bme1580I G_kGCm'C
Cuts at: 332 659 913 1306 2118 2211 3267 332
Size: 327 254 393 812 93 1056 1558
Fragments arranged by size:
1558 1056 812 393 327 254 93
BmgBI CAC'GTC
Cuts at: 453 453
Size: 4493
BmrI ACTGGGnnnn_n'
Cuts at: 1357 1705 1786 2121 4265 1357
Size: 348 81 335 2144 1585
Fragments arranged by size:
2144 1585 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 1366
Size: 4493
Bpu10I CC'TnA_GC
Cuts at: 1062 1062
Size: 4493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 1669 2548 3040 3338 1385
Size: 284 879 492 298 2540
Fragments arranged by size:
2540 879 492 298 284
BsaI GGTCTCn'nnnn_
Cuts at: 4197 4197
Size: 4493
BsaAI yAC'GTr
Cuts at: 1846 1846
Size: 4493
BsaBI GATnn'nnATC
Cuts at: 6 2007 4044 6
Size: 2001 2037 455
Fragments arranged by size:
2037 2001 455
BsaHI Gr'CG_yC
Cuts at: 193 1172 1924 2172 2874 4370 193
Size: 979 752 248 702 1496 316
Fragments arranged by size:
1496 979 752 702 316 248
BsaWI w'CCGG_w
Cuts at: 240 1682 2203 2881 3155 3302 3713 240
Size: 1442 521 678 274 147 411 1020
Fragments arranged by size:
1442 1020 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 175 1050 1120 136
Size: 39 875 70 3509
Fragments arranged by size:
3509 875 70 39
BseYI C'CCAG_C
Cuts at: 321 801 1918 2676 3253 3749 321
Size: 480 1117 758 577 496 1065
Fragments arranged by size:
1117 1065 758 577 496 480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1019 1019
Size: 4493
BsiEI CG_ry'CG
Cuts at: 104 419 530 2035 2081 3289 104
Size: 315 111 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 315 111 46
BsiHKAI G_wGCw'C
Cuts at: 332 842 1507 2285 2475 3267 332
Size: 510 665 778 190 792 1558
Fragments arranged by size:
1558 792 778 665 510 190
BsmI GAATG_Cn'
Cuts at: 571 1153 571
Size: 582 3911
Bsp1286I G_dGCh'C
Cuts at: 332 386 659 842 913 1102 1306 1507 2118
Size: 54 273 183 71 189 204 201 611
Cuts at: 2118 2211 2285 2475 2537 3267 332
Size: 93 74 190 62 730 1558
Fragments arranged by size:
1558 730 611 273 204 201 190 189
183 93 74 71 62 54
BspEI T'CCGG_A
Cuts at: 3713 3713
Size: 4493
BspHI T'CATG_A
Cuts at: 3669 3669
Size: 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1543 1757 2059 2440 1543
Size: 214 302 381 3596
Fragments arranged by size:
3596 381 302 214
BsrBI CCG'CTC
Cuts at: 50 268 965 1082 2785 2839 4323 4488 50
Size: 218 697 117 1703 54 1484 165 55
Fragments arranged by size:
1703 1484 697 218 165 117 55 54
BsrDI GCAATG_nn'
Cuts at: 2405 2405
Size: 4493
BsrFI r'CCGG_y
Cuts at: 299 418 2491 2672 2881 299
Size: 119 2073 181 209 1911
Fragments arranged by size:
2073 1911 209 181 119
BssSI C'ACGA_G
Cuts at: 706 2764 3122 706
Size: 2058 358 2077
Fragments arranged by size:
2077 2058 358
BstAPI GCAn_nnn'nTGC
Cuts at: 309 1870 309
Size: 1561 2932
BstBI TT'CG_AA
Cuts at: 2854 2854
Size: 4493
BstYI r'GATC_y
Cuts at: 1 1376 1518 1985 2008 2343 2589 3601 3687
Size: 1375 142 467 23 335 246 1012 86
Cuts at: 3687 3699 4045 1
Size: 12 346 449
Fragments arranged by size:
1375 1012 467 449 346 335 246 142
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1181 1181
Size: 4493
BtgI C'CryG_G
Cuts at: 107 107
Size: 4493
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2443 2624 2787 2443
Size: 181 163 4149
Fragments arranged by size:
4149 181 163
BtsI GCAGTG_nn'
Cuts at: 566 599 1736 566
Size: 33 1137 3323
Fragments arranged by size:
3323 1137 33
ClaI AT'CG_AT
Cuts at: 1466 1466
Size: 4493
DraI TTT'AAA
Cuts at: 1492 1492
Size: 4493
DrdI GACnn_nn'nnGTC
Cuts at: 2199 3057 2199
Size: 858 3635
EaeI y'GGCC_r
Cuts at: 416 507 527 2078 2252 2643 2670 4015 416
Size: 91 20 1551 174 391 27 1345 894
Fragments arranged by size:
1551 1345 894 391 174 91 27 20
EagI C'GGCC_G
Cuts at: 416 527 2078 416
Size: 111 1551 2831
Fragments arranged by size:
2831 1551 111
EarI CTCTTCn'nnn_
Cuts at: 1286 2516 2726 1286
Size: 1230 210 3053
Fragments arranged by size:
3053 1230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 607 2701 3011 3157 4303 607
Size: 2094 310 146 1146 797
Fragments arranged by size:
2094 1146 797 310 146
EcoICRI GAG'CTC
Cuts at: 1505 1505
Size: 4493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 164 199 1366 2318 2750 3497 3765 164
Size: 35 1167 952 432 747 268 892
Fragments arranged by size:
1167 952 892 747 432 268 35
EcoO109I rG'GnC_Cy
Cuts at: 585 858 910 1440 1632 585
Size: 273 52 530 192 3446
Fragments arranged by size:
3446 530 273 192 52
EcoRI G'AATT_C
Cuts at: 171 1497 171
Size: 1326 3167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2512 2512
Size: 4493
FspI TGC'GCA
Cuts at: 2274 2274
Size: 4493
HaeII r_GCGC'y
Cuts at: 1927 2175 3197 4176 1927
Size: 248 1022 979 2244
Fragments arranged by size:
2244 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2335 2365 2367 2397 2335
Size: 30 2 30 4431
Fragments arranged by size:
4431 30 30 2
HincII GTy'rAC
Cuts at: 1532 1532
Size: 4493
KasI G'GCGC_C
Cuts at: 1923 2171 1923
Size: 248 4245
KpnI G_GTAC'C
Cuts at: 1513 1513
Size: 4493
MluI A'CGCG_T
Cuts at: 3926 3926
Size: 4493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3164 3348 3164
Size: 184 4309
MscI TGG'CCA
Cuts at: 509 2254 509
Size: 1745 2748
MslI CAynn'nnrTG
Cuts at: 1333 2482 2609 1333
Size: 1149 127 3217
Fragments arranged by size:
3217 1149 127
MspA1I CmG'CkG
Cuts at: 252 339 415 805 1604 1918 2278 3291 3536
Size: 87 76 390 799 314 360 1013 245
Cuts at: 3536 252
Size: 1209
Fragments arranged by size:
1209 1013 799 390 360 314 245 87
76
NaeI GCC'GGC
Cuts at: 301 420 2674 301
Size: 119 2254 2120
Fragments arranged by size:
2254 2120 119
NarI GG'CG_CC
Cuts at: 1924 2172 1924
Size: 248 4245
NcoI C'CATG_G
Cuts at: 107 107
Size: 4493
NgoMIV G'CCGG_C
Cuts at: 299 418 2672 299
Size: 119 2254 2120
Fragments arranged by size:
2254 2120 119
NspI r_CATG'y
Cuts at: 686 1546 2046 2953 686
Size: 860 500 907 2226
Fragments arranged by size:
2226 907 860 500
PciI A'CATG_T
Cuts at: 2949 2949
Size: 4493
PmeI GTTT'AAAC
Cuts at: 1492 1492
Size: 4493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4461
PpuMI rG'GwC_Cy
Cuts at: 858 858
Size: 4493
PshAI GACnn'nnGTC
Cuts at: 1199 1199
Size: 4493
PspOMI G'GGCC_C
Cuts at: 909 909
Size: 4493
PstI C_TGCA'G
Cuts at: 875 1540 2225 875
Size: 665 685 3143
Fragments arranged by size:
3143 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4493
PvuII CAG'CTG
Cuts at: 339 1918 2278 339
Size: 1579 360 2554
Fragments arranged by size:
2554 1579 360
RsrII CG'GwC_CG
Cuts at: 2688 2688
Size: 4493
SacI G_AGCT'C
Cuts at: 1507 1507
Size: 4493
SalI G'TCGA_C
Cuts at: 1530 1530
Size: 4493
SapI GCTCTTCn'nnn_
Cuts at: 2516 2726 2516
Size: 210 4283
SbfI CC_TGCA'GG
Cuts at: 875 1540 875
Size: 665 3828
ScaI AGT'ACT
Cuts at: 406 4010 406
Size: 3604 889
SexAI A'CCwGG_T
Cuts at: 611 668 611
Size: 57 4436
SfcI C'TryA_G
Cuts at: 33 294 871 1536 2221 3214 3405 33
Size: 261 577 665 685 993 191 1121
Fragments arranged by size:
1121 993 685 665 577 261 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 434 526 434
Size: 92 4401
SfoI GGC'GCC
Cuts at: 1925 2173 1925
Size: 248 4245
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
SmaI CCC'GGG
Cuts at: 118 1515 118
Size: 1397 3096
SmlI C'TyrA_G
Cuts at: 1400 1648 2563 3055 3317 1400
Size: 248 915 492 262 2576
Fragments arranged by size:
2576 915 492 262 248
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4493
SphI G_CATG'C
Cuts at: 1546 2046 1546
Size: 500 3993
StuI AGG'CCT
Cuts at: 520 877 1179 520
Size: 357 302 3834
Fragments arranged by size:
3834 357 302
StyI C'CwwG_G
Cuts at: 107 432 515 878 1627 1981 107
Size: 325 83 363 749 354 2619
Fragments arranged by size:
2619 749 363 354 325 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2076 2209 2668 2877 2076
Size: 133 459 209 3692
Fragments arranged by size:
3692 459 209 133
TatI w'GTAC_w
Cuts at: 404 4008 404
Size: 3604 889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4461
Tth111I GACn'n_nGTC
Cuts at: 613 2290 613
Size: 1677 2816
XbaI T'CTAG_A
Cuts at: 1524 1524
Size: 4493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 487 666 760 1988 487
Size: 179 94 1228 2992
Fragments arranged by size:
2992 1228 179 94
XmaI C'CCGG_G
Cuts at: 116 1513 116
Size: 1397 3096
ZraI GAC'GTC
Cuts at: 1173 1173
Size: 4493
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmtI BsaXI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PsiI PspXI PsrI SacII SanDI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI