Restriction map for
pF1KB9872
(Circular) (Six-base) MAPSORT of: pF1KB9872.seq Check: 5368 from: 1 to: 4598
pF1KB9872 4598 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1636 1636
Size: 4598
Acc65I G'GTAC_C
Cuts at: 1614 1614
Size: 4598
AclI AA'CG_TT
Cuts at: 4438 4438
Size: 4598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 2423 2855 3602 3870 703
Size: 1720 432 747 268 1431
Fragments arranged by size:
1720 1431 747 432 268
AflIII A'CryG_T
Cuts at: 440 1238 1950 3054 4031 440
Size: 798 712 1104 977 1007
Fragments arranged by size:
1104 1007 977 798 712
AgeI A'CCGG_T
Cuts at: 2986 2986
Size: 4598
AlwNI CAG_nnn'CTG
Cuts at: 1050 1903 3470 1050
Size: 853 1567 2178
Fragments arranged by size:
2178 1567 853
ApaI G_GGCC'C
Cuts at: 602 602
Size: 4598
ApaLI G'TGCA_C
Cuts at: 3368 3368
Size: 4598
ApoI r'AATT_y
Cuts at: 1602 3951 4207 1602
Size: 2349 256 1993
Fragments arranged by size:
2349 1993 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4598
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
AvaI C'yCGr_G
Cuts at: 153 541 586 594 676 1618 153
Size: 388 45 8 82 942 3133
Fragments arranged by size:
3133 942 388 82 45 8
BamHI G'GATC_C
Cuts at: 1623 1623
Size: 4598
BanI G'GyrC_C
Cuts at: 706 916 1614 2028 2276 2311 706
Size: 210 698 414 248 35 2993
Fragments arranged by size:
2993 698 414 248 210 35
BanII G_rGCy'C
Cuts at: 595 602 1276 1612 2642 595
Size: 7 674 336 1030 2551
Fragments arranged by size:
2551 1030 674 336 7
BbeI G_GCGC'C
Cuts at: 2032 2280 2032
Size: 248 4350
BciVI GTATCCnnnnn_n'
Cuts at: 1192 1222 2496 3257 4598 1192
Size: 30 1274 761 1341 1192
Fragments arranged by size:
1341 1274 1192 761 30
BclI T'GATC_A
Cuts at: 227 2118 227
Size: 1891 2707
BglII A'GATC_T
Cuts at: 2113 4150 2113
Size: 2037 2561
BlpI GC'TnA_GC
Cuts at: 929 1710 929
Size: 781 3817
Bme1580I G_kGCm'C
Cuts at: 434 602 709 919 2223 2316 3372 434
Size: 168 107 210 1304 93 1056 1660
Fragments arranged by size:
1660 1304 1056 210 168 107 93
BmgBI CAC'GTC
Cuts at: 1395 1395
Size: 4598
BmrI ACTGGGnnnn_n'
Cuts at: 1810 1891 2226 4370 1810
Size: 81 335 2144 2038
Fragments arranged by size:
2144 2038 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4598
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1027 1161 685
Size: 342 134 4122
Fragments arranged by size:
4122 342 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1774 2653 3145 3443 247
Size: 1527 879 492 298 1402
Fragments arranged by size:
1527 1402 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4302 4302
Size: 4598
BsaAI yAC'GTr
Cuts at: 1951 1951
Size: 4598
BsaBI GATnn'nnATC
Cuts at: 6 232 2112 4149 6
Size: 226 1880 2037 455
Fragments arranged by size:
2037 1880 455 226
BsaHI Gr'CG_yC
Cuts at: 377 2029 2277 2979 4475 377
Size: 1652 248 702 1496 500
Fragments arranged by size:
1652 1496 702 500 248
BsaWI w'CCGG_w
Cuts at: 1787 2308 2986 3260 3407 3818 1787
Size: 521 678 274 147 411 2567
Fragments arranged by size:
2567 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 430 445 460 475 430
Size: 15 15 15 4553
Fragments arranged by size:
4553 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 493 538 541 466
Size: 27 45 3 4523
Fragments arranged by size:
4523 45 27 3
BseYI C'CCAG_C
Cuts at: 294 367 838 912 2023 2781 3358 3854 294
Size: 73 471 74 1111 758 577 496 1038
Fragments arranged by size:
1111 1038 758 577 496 471 74 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1154
Size: 4598
BsiEI CG_ry'CG
Cuts at: 104 149 357 408 2140 2186 3394 104
Size: 45 208 51 1732 46 1208 1308
Fragments arranged by size:
1732 1308 1208 208 51 46 45
BsiHKAI G_wGCw'C
Cuts at: 595 1612 2390 2580 3372 595
Size: 1017 778 190 792 1821
Fragments arranged by size:
1821 1017 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 247 627 661 247
Size: 380 34 4184
Fragments arranged by size:
4184 380 34
Bsp1286I G_dGCh'C
Cuts at: 434 595 602 709 919 1276 1612 2223 2316
Size: 161 7 107 210 357 336 611 93
Cuts at: 2316 2390 2580 2642 3372 434
Size: 74 190 62 730 1660
Fragments arranged by size:
1660 730 611 357 336 210 190 161
107 93 74 62 7
BspEI T'CCGG_A
Cuts at: 3818 3818
Size: 4598
BspHI T'CATG_A
Cuts at: 866 3774 866
Size: 2908 1690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1648 1862 2164 2545 1648
Size: 214 302 381 3701
Fragments arranged by size:
3701 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2890 2944 4428 4593 50
Size: 2840 54 1484 165 55
Fragments arranged by size:
2840 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2510 2510
Size: 4598
BsrFI r'CCGG_y
Cuts at: 362 420 2596 2777 2986 362
Size: 58 2176 181 209 1974
Fragments arranged by size:
2176 1974 209 181 58
BssSI C'ACGA_G
Cuts at: 205 2869 3227 205
Size: 2664 358 1576
Fragments arranged by size:
2664 1576 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1975 1975
Size: 4598
BstBI TT'CG_AA
Cuts at: 2959 2959
Size: 4598
BstXI CCAn_nnnn'nTGG
Cuts at: 969 969
Size: 4598
BstYI r'GATC_y
Cuts at: 1 1046 1623 2090 2113 2448 2694 3706 3792
Size: 1045 577 467 23 335 246 1012 86
Cuts at: 3792 3804 4150 1
Size: 12 346 449
Fragments arranged by size:
1045 1012 577 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 334 979 334
Size: 645 3953
BtgI C'CryG_G
Cuts at: 485 731 485
Size: 246 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 597 1272 2548 2729 2892 597
Size: 675 1276 181 163 2303
Fragments arranged by size:
2303 1276 675 181 163
BtsI GCAGTG_nn'
Cuts at: 1142 1544 1841 1142
Size: 402 297 3899
Fragments arranged by size:
3899 402 297
DraI TTT'AAA
Cuts at: 1597 1597
Size: 4598
DrdI GACnn_nn'nnGTC
Cuts at: 117 2304 3162 117
Size: 2187 858 1553
Fragments arranged by size:
2187 1553 858
EaeI y'GGCC_r
Cuts at: 146 405 887 971 2183 2357 2748 2775 4120
Size: 259 482 84 1212 174 391 27 1345
Cuts at: 4120 146
Size: 624
Fragments arranged by size:
1345 1212 624 482 391 259 174 84
27
EagI C'GGCC_G
Cuts at: 146 405 2183 146
Size: 259 1778 2561
Fragments arranged by size:
2561 1778 259
EarI CTCTTCn'nnn_
Cuts at: 580 1111 1367 1475 2621 2831 580
Size: 531 256 108 1146 210 2347
Fragments arranged by size:
2347 1146 531 256 210 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1168 2806 3116 3262 4408 1168
Size: 1638 310 146 1146 1358
Fragments arranged by size:
1638 1358 1146 310 146
EcoICRI GAG'CTC
Cuts at: 593 1610 593
Size: 1017 3581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 703 1027 1161 2423 2855 3602 3870 685
Size: 18 324 134 1262 432 747 268 1413
Fragments arranged by size:
1413 1262 747 432 324 268 134 18
EcoO109I rG'GnC_Cy
Cuts at: 598 1038 1737 598
Size: 440 699 3459
Fragments arranged by size:
3459 699 440
EcoRI G'AATT_C
Cuts at: 1602 1602
Size: 4598
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 867 2617 867
Size: 1750 2848
FspI TGC'GCA
Cuts at: 2379 2379
Size: 4598
HaeII r_GCGC'y
Cuts at: 534 561 2032 2280 3302 4281 534
Size: 27 1471 248 1022 979 851
Fragments arranged by size:
1471 1022 979 851 248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 1250 1282 2440 2470 2472 2502 430
Size: 32 788 32 1158 30 2 30 2526
Fragments arranged by size:
2526 1158 788 32 32 30 30 2
HincII GTy'rAC
Cuts at: 426 1637 426
Size: 1211 3387
KasI G'GCGC_C
Cuts at: 2028 2276 2028
Size: 248 4350
KpnI G_GTAC'C
Cuts at: 1618 1618
Size: 4598
MfeI C'AATT_G
Cuts at: 1253 1304 1536 1253
Size: 51 232 4315
Fragments arranged by size:
4315 232 51
MluI A'CGCG_T
Cuts at: 4031 4031
Size: 4598
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 773 3269 3453 773
Size: 2496 184 1918
Fragments arranged by size:
2496 1918 184
MscI TGG'CCA
Cuts at: 889 973 2359 889
Size: 84 1386 3128
Fragments arranged by size:
3128 1386 84
MslI CAynn'nnrTG
Cuts at: 112 2587 2714 112
Size: 2475 127 1996
Fragments arranged by size:
2475 1996 127
MspA1I CmG'CkG
Cuts at: 1709 2023 2383 3396 3641 1709
Size: 314 360 1013 245 2666
Fragments arranged by size:
2666 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2779 2779
Size: 4598
NarI GG'CG_CC
Cuts at: 2029 2277 2029
Size: 248 4350
NcoI C'CATG_G
Cuts at: 485 485
Size: 4598
NgoMIV G'CCGG_C
Cuts at: 2777 2777
Size: 4598
NotI GC'GGCC_GC
Cuts at: 405 405
Size: 4598
NspI r_CATG'y
Cuts at: 318 444 1242 1651 2151 3058 318
Size: 126 798 409 500 907 1858
Fragments arranged by size:
1858 907 798 500 409 126
PciI A'CATG_T
Cuts at: 440 1238 3054 440
Size: 798 1816 1984
Fragments arranged by size:
1984 1816 798
PflMI CCAn_nnn'nTGG
Cuts at: 1050 1050
Size: 4598
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 4598
PmeI GTTT'AAAC
Cuts at: 1597 1597
Size: 4598
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 3737
Size: 32 4566
PshAI GACnn'nnGTC
Cuts at: 1430 1430
Size: 4598
PspOMI G'GGCC_C
Cuts at: 598 598
Size: 4598
PstI C_TGCA'G
Cuts at: 1645 2330 1645
Size: 685 3913
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4598
PvuII CAG'CTG
Cuts at: 2023 2383 2023
Size: 360 4238
RsrII CG'GwC_CG
Cuts at: 2793 2793
Size: 4598
SacI G_AGCT'C
Cuts at: 595 1612 595
Size: 1017 3581
SalI G'TCGA_C
Cuts at: 1635 1635
Size: 4598
SapI GCTCTTCn'nnn_
Cuts at: 580 1367 2621 2831 580
Size: 787 1254 210 2347
Fragments arranged by size:
2347 1254 787 210
SbfI CC_TGCA'GG
Cuts at: 1645 1645
Size: 4598
ScaI AGT'ACT
Cuts at: 346 4115 346
Size: 3769 829
SfcI C'TryA_G
Cuts at: 33 1223 1641 2326 3319 3510 33
Size: 1190 418 685 993 191 1121
Fragments arranged by size:
1190 1121 993 685 418 191
SfoI GGC'GCC
Cuts at: 2030 2278 2030
Size: 248 4350
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4598
SmaI CCC'GGG
Cuts at: 155 1620 155
Size: 1465 3133
SmlI C'TyrA_G
Cuts at: 262 1753 2668 3160 3422 262
Size: 1491 915 492 262 1438
Fragments arranged by size:
1491 1438 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4598
SphI G_CATG'C
Cuts at: 318 1651 2151 318
Size: 1333 500 2765
Fragments arranged by size:
2765 1333 500
StyI C'CwwG_G
Cuts at: 485 1732 2086 485
Size: 1247 354 2997
Fragments arranged by size:
2997 1247 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1600 2181 2314 2773 2982 1600
Size: 581 133 459 209 3216
Fragments arranged by size:
3216 581 459 209 133
TatI w'GTAC_w
Cuts at: 344 1139 1466 4113 344
Size: 795 327 2647 829
Fragments arranged by size:
2647 829 795 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 772 804 1384 1416 4115 4147 772
Size: 32 580 32 2699 32 1223
Fragments arranged by size:
2699 1223 580 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2395 2395
Size: 4598
XbaI T'CTAG_A
Cuts at: 1629 1629
Size: 4598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 166 291 2093 166
Size: 125 1802 2671
Fragments arranged by size:
2671 1802 125
XmaI C'CCGG_G
Cuts at: 153 1618 153
Size: 1465 3133
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PflMI PfoI PmeI
PpiI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NruI NsiI PacI PasI PmlI
PpuMI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI