Restriction map for
pF1KB9874
(Circular) (Six-base) MAPSORT of: pF1KB9874.seq Check: 7769 from: 1 to: 4658
pF1KB9874 4658 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 339 339
Size: 4658
AccI GT'mk_AC
Cuts at: 380 1696 380
Size: 1316 3342
Acc65I G'GTAC_C
Cuts at: 1674 1674
Size: 4658
AclI AA'CG_TT
Cuts at: 4498 4498
Size: 4658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 952 1247 1630 2483 2915 3662 3930 251
Size: 701 295 383 853 432 747 268 979
Fragments arranged by size:
979 853 747 701 432 383 295 268
AflIII A'CryG_T
Cuts at: 2010 3114 4091 2010
Size: 1104 977 2577
Fragments arranged by size:
2577 1104 977
AgeI A'CCGG_T
Cuts at: 3046 3046
Size: 4658
AleI CACnn'nnGTG
Cuts at: 577 991 577
Size: 414 4244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1458 1490 1458
Size: 32 4626
AlwNI CAG_nnn'CTG
Cuts at: 1565 1963 3530 1565
Size: 398 1567 2693
Fragments arranged by size:
2693 1567 398
ApaLI G'TGCA_C
Cuts at: 3428 3428
Size: 4658
ApoI r'AATT_y
Cuts at: 794 868 924 1008 1662 4011 4267 794
Size: 74 56 84 654 2349 256 1185
Fragments arranged by size:
2349 1185 654 256 84 74 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 4658
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
AvaI C'yCGr_G
Cuts at: 1678 1678
Size: 4658
AvrII C'CTAG_G
Cuts at: 484 804 484
Size: 320 4338
BamHI G'GATC_C
Cuts at: 1683 1683
Size: 4658
BanI G'GyrC_C
Cuts at: 1674 2088 2336 2371 1674
Size: 414 248 35 3961
Fragments arranged by size:
3961 414 248 35
BanII G_rGCy'C
Cuts at: 1672 2702 1672
Size: 1030 3628
BbeI G_GCGC'C
Cuts at: 2092 2340 2092
Size: 248 4410
BbsI GAAGACnn'nnnn_
Cuts at: 356 356
Size: 4658
BciVI GTATCCnnnnn_n'
Cuts at: 2556 3317 4658 2556
Size: 761 1341 2556
Fragments arranged by size:
2556 1341 761
BclI T'GATC_A
Cuts at: 2178 2178
Size: 4658
BfrBI ATG'CAT
Cuts at: 394 1067 394
Size: 673 3985
BglII A'GATC_T
Cuts at: 176 1641 2173 4210 176
Size: 1465 532 2037 624
Fragments arranged by size:
2037 1465 624 532
BlpI GC'TnA_GC
Cuts at: 1770 1770
Size: 4658
Bme1580I G_kGCm'C
Cuts at: 1459 2283 2376 3432 1459
Size: 824 93 1056 2685
Fragments arranged by size:
2685 1056 824 93
BmrI ACTGGGnnnn_n'
Cuts at: 348 1870 1951 2286 4430 348
Size: 1522 81 335 2144 576
Fragments arranged by size:
2144 1522 576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 4658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 481 544 160
Size: 321 63 4274
Fragments arranged by size:
4274 321 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 699 1420 1834 2713 3205 3503 436
Size: 263 721 414 879 492 298 1591
Fragments arranged by size:
1591 879 721 492 414 298 263
BsaI GGTCTCn'nnnn_
Cuts at: 4362 4362
Size: 4658
BsaAI yAC'GTr
Cuts at: 1596 2011 1596
Size: 415 4243
BsaBI GATnn'nnATC
Cuts at: 6 959 2172 4209 6
Size: 953 1213 2037 455
Fragments arranged by size:
2037 1213 953 455
BsaHI Gr'CG_yC
Cuts at: 2089 2337 3039 4535 2089
Size: 248 702 1496 2212
Fragments arranged by size:
2212 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1847 2368 3046 3320 3467 3878 1847
Size: 521 678 274 147 411 2627
Fragments arranged by size:
2627 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 142 172 457 487 1458 1488 142
Size: 30 285 30 971 30 3312
Fragments arranged by size:
3312 971 285 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 510 478
Size: 32 4626
BseYI C'CCAG_C
Cuts at: 2083 2841 3418 3914 2083
Size: 758 577 496 2827
Fragments arranged by size:
2827 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2200 2246 3454 104
Size: 2096 46 1208 1308
Fragments arranged by size:
2096 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 916 1672 2450 2640 3432 916
Size: 756 778 190 792 2142
Fragments arranged by size:
2142 792 778 756 190
BsmI GAATG_Cn'
Cuts at: 212 212
Size: 4658
Bsp1286I G_dGCh'C
Cuts at: 916 1459 1672 2283 2376 2450 2640 2702 3432
Size: 543 213 611 93 74 190 62 730
Cuts at: 3432 916
Size: 2142
Fragments arranged by size:
2142 730 611 543 213 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3878 3878
Size: 4658
BspHI T'CATG_A
Cuts at: 620 3834 620
Size: 3214 1444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 339 1708 1922 2224 2605 339
Size: 1369 214 302 381 2392
Fragments arranged by size:
2392 1369 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1271 2950 3004 4488 4653 50
Size: 1221 1679 54 1484 165 55
Fragments arranged by size:
1679 1484 1221 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2570 2570
Size: 4658
BsrFI r'CCGG_y
Cuts at: 2656 2837 3046 2656
Size: 181 209 4268
Fragments arranged by size:
4268 209 181
BssSI C'ACGA_G
Cuts at: 2929 3287 2929
Size: 358 4300
BstAPI GCAn_nnn'nTGC
Cuts at: 2035 2035
Size: 4658
BstBI TT'CG_AA
Cuts at: 204 3019 204
Size: 2815 1843
BstEII G'GTnAC_C
Cuts at: 1198 1264 1415 1429 1198
Size: 66 151 14 4427
Fragments arranged by size:
4427 151 66 14
BstYI r'GATC_y
Cuts at: 1 176 1641 1683 2150 2173 2508 2754 3766
Size: 175 1465 42 467 23 335 246 1012
Cuts at: 3766 3852 3864 4210 1
Size: 86 12 346 449
Fragments arranged by size:
1465 1012 467 449 346 335 246 175
86 42 23 12
BtgI C'CryG_G
Cuts at: 107 675 1363 107
Size: 568 688 3402
Fragments arranged by size:
3402 688 568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2608 2789 2952 2608
Size: 181 163 4314
Fragments arranged by size:
4314 181 163
BtsI GCAGTG_nn'
Cuts at: 986 998 1514 1901 986
Size: 12 516 387 3743
Fragments arranged by size:
3743 516 387 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 150 185 150
Size: 35 4623
DraI TTT'AAA
Cuts at: 813 1007 1657 813
Size: 194 650 3814
Fragments arranged by size:
3814 650 194
DraIII CAC_nnn'GTG
Cuts at: 1599 1599
Size: 4658
DrdI GACnn_nn'nnGTC
Cuts at: 709 2364 3222 709
Size: 1655 858 2145
Fragments arranged by size:
2145 1655 858
EaeI y'GGCC_r
Cuts at: 678 2243 2417 2808 2835 4180 678
Size: 1565 174 391 27 1345 1156
Fragments arranged by size:
1565 1345 1156 391 174 27
EagI C'GGCC_G
Cuts at: 2243 2243
Size: 4658
EarI CTCTTCn'nnn_
Cuts at: 416 2681 2891 416
Size: 2265 210 2183
Fragments arranged by size:
2265 2183 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2866 3176 3322 4468 2866
Size: 310 146 1146 3056
Fragments arranged by size:
3056 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1670 1670
Size: 4658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 251 481 544 952 1247 1630 2483 2915
Size: 91 230 63 408 295 383 853 432
Cuts at: 2915 3662 3930 160
Size: 747 268 888
Fragments arranged by size:
888 853 747 432 408 383 295 268
230 91 63
EcoNI CCTnn'n_nnAGG
Cuts at: 1424 1481 1424
Size: 57 4601
EcoO109I rG'GnC_Cy
Cuts at: 1797 1797
Size: 4658
EcoRI G'AATT_C
Cuts at: 868 1662 868
Size: 794 3864
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4658
FspI TGC'GCA
Cuts at: 2439 2439
Size: 4658
HaeII r_GCGC'y
Cuts at: 2092 2340 3362 4341 2092
Size: 248 1022 979 2409
Fragments arranged by size:
2409 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 426 458 1480 1512 2500 2530 2532
Size: 32 156 32 1022 32 988 30 2
Cuts at: 2532 2562 238
Size: 30 2334
Fragments arranged by size:
2334 1022 988 156 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1697 1697
Size: 4658
KasI G'GCGC_C
Cuts at: 2088 2336 2088
Size: 248 4410
KpnI G_GTAC'C
Cuts at: 1678 1678
Size: 4658
MluI A'CGCG_T
Cuts at: 4091 4091
Size: 4658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 594 3329 3513 594
Size: 2735 184 1739
Fragments arranged by size:
2735 1739 184
MscI TGG'CCA
Cuts at: 680 2419 680
Size: 1739 2919
MslI CAynn'nnrTG
Cuts at: 577 991 2647 2774 577
Size: 414 1656 127 2461
Fragments arranged by size:
2461 1656 414 127
MspA1I CmG'CkG
Cuts at: 229 522 1365 1769 2083 2443 3456 3701 229
Size: 293 843 404 314 360 1013 245 1186
Fragments arranged by size:
1186 1013 843 404 360 314 293 245
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 4658
NarI GG'CG_CC
Cuts at: 2089 2337 2089
Size: 248 4410
NcoI C'CATG_G
Cuts at: 107 675 107
Size: 568 4090
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 4658
NsiI A_TGCA'T
Cuts at: 396 1069 396
Size: 673 3985
NspI r_CATG'y
Cuts at: 1711 2211 3118 1711
Size: 500 907 3251
Fragments arranged by size:
3251 907 500
PciI A'CATG_T
Cuts at: 3114 3114
Size: 4658
PflMI CCAn_nnn'nTGG
Cuts at: 1209 1209
Size: 4658
PmeI GTTT'AAAC
Cuts at: 1657 1657
Size: 4658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1240 1272 1458 1490 3797 3829 1240
Size: 32 186 32 2307 32 2069
Fragments arranged by size:
2307 2069 186 32 32 32
PsiI TTA'TAA
Cuts at: 959 959
Size: 4658
PstI C_TGCA'G
Cuts at: 292 350 993 1705 2390 292
Size: 58 643 712 685 2560
Fragments arranged by size:
2560 712 685 643 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4658
PvuII CAG'CTG
Cuts at: 229 522 2083 2443 229
Size: 293 1561 360 2444
Fragments arranged by size:
2444 1561 360 293
RsrII CG'GwC_CG
Cuts at: 2853 2853
Size: 4658
SacI G_AGCT'C
Cuts at: 1672 1672
Size: 4658
SacII CC_GC'GG
Cuts at: 1366 1366
Size: 4658
SalI G'TCGA_C
Cuts at: 1695 1695
Size: 4658
SapI GCTCTTCn'nnn_
Cuts at: 2681 2891 2681
Size: 210 4448
SbfI CC_TGCA'GG
Cuts at: 1705 1705
Size: 4658
ScaI AGT'ACT
Cuts at: 1119 4175 1119
Size: 3056 1602
SfcI C'TryA_G
Cuts at: 33 288 346 758 989 1289 1394 1701 2386
Size: 255 58 412 231 300 105 307 685
Cuts at: 2386 3379 3570 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 412 307 300 255 231
191 105 58
SfoI GGC'GCC
Cuts at: 2090 2338 2090
Size: 248 4410
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
SmaI CCC'GGG
Cuts at: 1680 1680
Size: 4658
SmlI C'TyrA_G
Cuts at: 415 714 1435 1813 2728 3220 3482 415
Size: 299 721 378 915 492 262 1591
Fragments arranged by size:
1591 915 721 492 378 299 262
SpeI A'CTAG_T
Cuts at: 67 1120 67
Size: 1053 3605
SphI G_CATG'C
Cuts at: 1711 2211 1711
Size: 500 4158
SspI AAT'ATT
Cuts at: 737 1178 737
Size: 441 4217
StuI AGG'CCT
Cuts at: 313 313
Size: 4658
StyI C'CwwG_G
Cuts at: 107 126 484 675 804 1792 2146 107
Size: 19 358 191 129 988 354 2619
Fragments arranged by size:
2619 988 358 354 191 129 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 208 2241 2374 2833 3042 208
Size: 2033 133 459 209 1824
Fragments arranged by size:
2033 1824 459 209 133
TatI w'GTAC_w
Cuts at: 1089 1117 4173 1089
Size: 28 3056 1574
Fragments arranged by size:
3056 1574 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 142 174 1585 1617 4175 4207 142
Size: 32 1411 32 2558 32 593
Fragments arranged by size:
2558 1411 593 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2455 2455
Size: 4658
XbaI T'CTAG_A
Cuts at: 1258 1689 1258
Size: 431 4227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2153 2153
Size: 4658
XmaI C'CCGG_G
Cuts at: 1678 1678
Size: 4658
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI BaeI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI XmnI ZraI