Restriction map for
pF1KB9875
(Circular) (Six-base) MAPSORT of: pF1KB9875.seq Check: 3868 from: 1 to: 4694
pF1KB9875 4694 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1732 1732
Size: 4694
Acc65I G'GTAC_C
Cuts at: 1398 1710 1398
Size: 312 4382
AclI AA'CG_TT
Cuts at: 4534 4534
Size: 4694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 530 691 1181 1271 2519 2951 3698 3966
Size: 232 161 490 90 1248 432 747 268
Cuts at: 3966 298
Size: 1026
Fragments arranged by size:
1248 1026 747 490 432 268 232 161
90
AflIII A'CryG_T
Cuts at: 2046 3150 4127 2046
Size: 1104 977 2613
Fragments arranged by size:
2613 1104 977
AgeI A'CCGG_T
Cuts at: 3082 3082
Size: 4694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 877 877
Size: 4694
AlwNI CAG_nnn'CTG
Cuts at: 1999 3566 1999
Size: 1567 3127
ApaLI G'TGCA_C
Cuts at: 3464 3464
Size: 4694
ApoI r'AATT_y
Cuts at: 174 670 916 1001 1255 1300 1698 4047 4303
Size: 496 246 85 254 45 398 2349 256
Cuts at: 4303 174
Size: 565
Fragments arranged by size:
2349 565 496 398 256 254 246 85
45
AseI AT'TA_AT
Cuts at: 20 533 20
Size: 513 4181
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1714 1714
Size: 4694
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1382 1415 1382
Size: 33 4661
BamHI G'GATC_C
Cuts at: 1719 1719
Size: 4694
BanI G'GyrC_C
Cuts at: 1398 1710 2124 2372 2407 1398
Size: 312 414 248 35 3685
Fragments arranged by size:
3685 414 312 248 35
BanII G_rGCy'C
Cuts at: 1421 1569 1708 2738 1421
Size: 148 139 1030 3377
Fragments arranged by size:
3377 1030 148 139
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 1250 1250
Size: 4694
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3353 4694 2592
Size: 761 1341 2592
Fragments arranged by size:
2592 1341 761
BclI T'GATC_A
Cuts at: 2214 2214
Size: 4694
BglII A'GATC_T
Cuts at: 2209 4246 2209
Size: 2037 2657
BlpI GC'TnA_GC
Cuts at: 1806 1806
Size: 4694
Bme1580I G_kGCm'C
Cuts at: 1463 2319 2412 3468 1463
Size: 856 93 1056 2689
Fragments arranged by size:
2689 1056 856 93
BmrI ACTGGGnnnn_n'
Cuts at: 1473 1906 1987 2322 4466 1473
Size: 433 81 335 2144 1701
Fragments arranged by size:
2144 1701 433 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 4694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 867 257
Size: 610 4084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1870 2749 3241 3539 1870
Size: 879 492 298 3025
Fragments arranged by size:
3025 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1009 4398 1009
Size: 3389 1305
BsaAI yAC'GTr
Cuts at: 2047 2047
Size: 4694
BsaBI GATnn'nnATC
Cuts at: 6 2208 4245 6
Size: 2202 2037 455
Fragments arranged by size:
2202 2037 455
BsaHI Gr'CG_yC
Cuts at: 2125 2373 3075 4571 2125
Size: 248 702 1496 2248
Fragments arranged by size:
2248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3356 3503 3914 1883
Size: 521 678 274 147 411 2663
Fragments arranged by size:
2663 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 308 308
Size: 4694
BseYI C'CCAG_C
Cuts at: 330 840 2119 2877 3454 3950 330
Size: 510 1279 758 577 496 1074
Fragments arranged by size:
1279 1074 758 577 510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 462 1289 462
Size: 827 3867
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3490 104
Size: 2132 46 1208 1308
Fragments arranged by size:
2132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1421 1569 1708 2486 2676 3468 1421
Size: 148 139 778 190 792 2647
Fragments arranged by size:
2647 792 778 190 148 139
Bsp1286I G_dGCh'C
Cuts at: 1421 1463 1569 1708 2319 2412 2486 2676 2738
Size: 42 106 139 611 93 74 190 62
Cuts at: 2738 3468 1421
Size: 730 2647
Fragments arranged by size:
2647 730 611 190 139 106 93 74
62 42
BspEI T'CCGG_A
Cuts at: 3914 3914
Size: 4694
BspHI T'CATG_A
Cuts at: 3870 3870
Size: 4694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1744 1958 2260 2641 1744
Size: 214 302 381 3797
Fragments arranged by size:
3797 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1548 2606 1548
Size: 1058 3636
BsrFI r'CCGG_y
Cuts at: 2692 2873 3082 2692
Size: 181 209 4304
Fragments arranged by size:
4304 209 181
BsrGI T'GTAC_A
Cuts at: 542 806 542
Size: 264 4430
BssSI C'ACGA_G
Cuts at: 2965 3323 2965
Size: 358 4336
BstAPI GCAn_nnn'nTGC
Cuts at: 2071 2071
Size: 4694
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstXI CCAn_nnnn'nTGG
Cuts at: 537 537
Size: 4694
BstYI r'GATC_y
Cuts at: 1 223 1218 1485 1719 2186 2209 2544 2790
Size: 222 995 267 234 467 23 335 246
Cuts at: 2790 3802 3888 3900 4246 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 995 467 449 346 335 267 246
234 222 86 23 12
BtgI C'CryG_G
Cuts at: 387 387
Size: 4694
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2644 2825 2988 2644
Size: 181 163 4350
Fragments arranged by size:
4350 181 163
BtsI GCAGTG_nn'
Cuts at: 1937 1937
Size: 4694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1221 1256 1221
Size: 35 4659
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3258 2400
Size: 858 3836
EaeI y'GGCC_r
Cuts at: 2279 2453 2844 2871 4216 2279
Size: 174 391 27 1345 2757
Fragments arranged by size:
2757 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 326 1172 2717 2927 326
Size: 846 1545 210 2093
Fragments arranged by size:
2093 1545 846 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3212 3358 4504 2902
Size: 310 146 1146 3092
Fragments arranged by size:
3092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1419 1567 1706 1419
Size: 148 139 4407
Fragments arranged by size:
4407 148 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 298 530 691 867 1181 1271 2519 2951
Size: 41 232 161 176 314 90 1248 432
Cuts at: 2951 3698 3966 257
Size: 747 268 985
Fragments arranged by size:
1248 985 747 432 314 268 232 176
161 90 41
EcoO109I rG'GnC_Cy
Cuts at: 1833 1833
Size: 4694
EcoRI G'AATT_C
Cuts at: 1698 1698
Size: 4694
EcoRV GAT'ATC
Cuts at: 1386 1386
Size: 4694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1683 2713 1683
Size: 1030 3664
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 2128 2376 3398 4377 2128
Size: 248 1022 979 2445
Fragments arranged by size:
2445 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 703 735 2536 2566 2568 2598 703
Size: 32 1801 30 2 30 2799
Fragments arranged by size:
2799 1801 32 30 30 2
HincII GTy'rAC
Cuts at: 1733 1733
Size: 4694
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1402 1714 1402
Size: 312 4382
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3365 3549 3365
Size: 184 4510
MscI TGG'CCA
Cuts at: 2455 2455
Size: 4694
MslI CAynn'nnrTG
Cuts at: 251 266 535 1454 2683 2810 251
Size: 15 269 919 1229 127 2135
Fragments arranged by size:
2135 1229 919 269 127 15
MspA1I CmG'CkG
Cuts at: 844 1805 2119 2479 3492 3737 844
Size: 961 314 360 1013 245 1801
Fragments arranged by size:
1801 1013 961 360 314 245
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 4694
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NcoI C'CATG_G
Cuts at: 387 387
Size: 4694
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 4694
NspI r_CATG'y
Cuts at: 594 1364 1552 1747 2247 3154 594
Size: 770 188 195 500 907 2134
Fragments arranged by size:
2134 907 770 500 195 188
PasI CC'CwG_GG
Cuts at: 831 831
Size: 4694
PciI A'CATG_T
Cuts at: 3150 3150
Size: 4694
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1487 1519 3833 3865 1487
Size: 32 2314 32 2316
Fragments arranged by size:
2316 2314 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 488 520 1617 1649 488
Size: 32 1097 32 3533
Fragments arranged by size:
3533 1097 32 32
PstI C_TGCA'G
Cuts at: 119 1741 2426 119
Size: 1622 685 2387
Fragments arranged by size:
2387 1622 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 844 2119 2479 844
Size: 1275 360 3059
Fragments arranged by size:
3059 1275 360
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1421 1569 1708 1421
Size: 148 139 4407
Fragments arranged by size:
4407 148 139
SalI G'TCGA_C
Cuts at: 1731 1731
Size: 4694
SapI GCTCTTCn'nnn_
Cuts at: 1172 2717 2927 1172
Size: 1545 210 2939
Fragments arranged by size:
2939 1545 210
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SfcI C'TryA_G
Cuts at: 33 115 1737 2422 3415 3606 33
Size: 82 1622 685 993 191 1121
Fragments arranged by size:
1622 1121 993 685 191 82
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 1849 2764 3256 3518 1849
Size: 915 492 262 3025
Fragments arranged by size:
3025 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 1747 2247 1747
Size: 500 4194
StyI C'CwwG_G
Cuts at: 387 1828 2182 387
Size: 1441 354 2899
Fragments arranged by size:
2899 1441 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 553 665 2277 2410 2869 3078 553
Size: 112 1612 133 459 209 2169
Fragments arranged by size:
2169 1612 459 209 133 112
TatI w'GTAC_w
Cuts at: 542 806 4209 542
Size: 264 3403 1027
Fragments arranged by size:
3403 1027 264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 4662
Tth111I GACn'n_nGTC
Cuts at: 2491 2491
Size: 4694
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 4694
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI