Restriction map for
pF1KB9876
(Circular) (Six-base) MAPSORT of: pF1KB9876.seq Check: 1096 from: 1 to: 4739
pF1KB9876 4739 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1120 1120
Size: 4739
AccI GT'mk_AC
Cuts at: 1777 1777
Size: 4739
Acc65I G'GTAC_C
Cuts at: 675 1755 675
Size: 1080 3659
AclI AA'CG_TT
Cuts at: 4579 4579
Size: 4739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 602 683 1444 1580 2564 2996 3743 4011
Size: 342 81 761 136 984 432 747 268
Cuts at: 4011 260
Size: 988
Fragments arranged by size:
988 984 761 747 432 342 268 136
81
AflIII A'CryG_T
Cuts at: 362 2091 3195 4172 362
Size: 1729 1104 977 929
Fragments arranged by size:
1729 1104 977 929
AgeI A'CCGG_T
Cuts at: 3127 3127
Size: 4739
AhdI GACnn_n'nnGTC
Cuts at: 443 545 1653 443
Size: 102 1108 3529
Fragments arranged by size:
3529 1108 102
AlwNI CAG_nnn'CTG
Cuts at: 2044 3611 2044
Size: 1567 3172
ApaI G_GGCC'C
Cuts at: 1193 1193
Size: 4739
ApaLI G'TGCA_C
Cuts at: 3509 3509
Size: 4739
ApoI r'AATT_y
Cuts at: 153 267 318 1069 1371 1743 4092 4348 153
Size: 114 51 751 302 372 2349 256 544
Fragments arranged by size:
2349 751 544 372 302 256 114 51
AseI AT'TA_AT
Cuts at: 20 20
Size: 4739
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
AvaI C'yCGr_G
Cuts at: 1759 1759
Size: 4739
BamHI G'GATC_C
Cuts at: 1764 1764
Size: 4739
BanI G'GyrC_C
Cuts at: 675 1755 2169 2417 2452 675
Size: 1080 414 248 35 2962
Fragments arranged by size:
2962 1080 414 248 35
BanII G_rGCy'C
Cuts at: 493 1193 1753 2783 493
Size: 700 560 1030 2449
Fragments arranged by size:
2449 1030 700 560
BbeI G_GCGC'C
Cuts at: 2173 2421 2173
Size: 248 4491
BbsI GAAGACnn'nnnn_
Cuts at: 455 701 991 1106 1649 1687 455
Size: 246 290 115 543 38 3507
Fragments arranged by size:
3507 543 290 246 115 38
BbvCI CC'TCA_GC
Cuts at: 1009 1009
Size: 4739
BciVI GTATCCnnnnn_n'
Cuts at: 2637 3398 4739 2637
Size: 761 1341 2637
Fragments arranged by size:
2637 1341 761
BclI T'GATC_A
Cuts at: 2259 2259
Size: 4739
BglII A'GATC_T
Cuts at: 198 2254 4291 198
Size: 2056 2037 646
Fragments arranged by size:
2056 2037 646
BlpI GC'TnA_GC
Cuts at: 1851 1851
Size: 4739
Bme1580I G_kGCm'C
Cuts at: 1193 2364 2457 3513 1193
Size: 1171 93 1056 2419
Fragments arranged by size:
2419 1171 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 506 1951 2032 2367 4511 506
Size: 1445 81 335 2144 734
Fragments arranged by size:
2144 1445 734 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3106
Size: 4739
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 590
Size: 4739
Bpu10I CC'TnA_GC
Cuts at: 1009 1009
Size: 4739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1915 2794 3286 3584 1915
Size: 879 492 298 3070
Fragments arranged by size:
3070 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 570 4443 570
Size: 3873 866
BsaAI yAC'GTr
Cuts at: 363 2092 363
Size: 1729 3010
BsaBI GATnn'nnATC
Cuts at: 6 191 197 2253 4290 6
Size: 185 6 2056 2037 455
Fragments arranged by size:
2056 2037 455 185 6
BsaHI Gr'CG_yC
Cuts at: 2170 2418 3120 4616 2170
Size: 248 702 1496 2293
Fragments arranged by size:
2293 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1928 2449 3127 3401 3548 3959 1928
Size: 521 678 274 147 411 2708
Fragments arranged by size:
2708 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 548 578 548
Size: 30 4709
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 587 193
Size: 394 4345
BseYI C'CCAG_C
Cuts at: 1094 2164 2922 3499 3995 1094
Size: 1070 758 577 496 1838
Fragments arranged by size:
1838 1070 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 436 2281 2327 3535 104
Size: 332 1845 46 1208 1308
Fragments arranged by size:
1845 1308 1208 332 46
BsiHKAI G_wGCw'C
Cuts at: 493 1514 1753 2531 2721 3513 493
Size: 1021 239 778 190 792 1719
Fragments arranged by size:
1719 1021 792 778 239 190
Bsp1286I G_dGCh'C
Cuts at: 493 1193 1514 1753 2364 2457 2531 2721 2783
Size: 700 321 239 611 93 74 190 62
Cuts at: 2783 3513 493
Size: 730 1719
Fragments arranged by size:
1719 730 700 611 321 239 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3959 3959
Size: 4739
BspHI T'CATG_A
Cuts at: 3915 3915
Size: 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1120 1789 2003 2305 2686 1120
Size: 669 214 302 381 3173
Fragments arranged by size:
3173 669 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3031 3085 4569 4734 50
Size: 2981 54 1484 165 55
Fragments arranged by size:
2981 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 866 2651 866
Size: 1785 2954
BsrFI r'CCGG_y
Cuts at: 411 1127 1475 2737 2918 3127 411
Size: 716 348 1262 181 209 2023
Fragments arranged by size:
2023 1262 716 348 209 181
BsrGI T'GTAC_A
Cuts at: 225 1173 225
Size: 948 3791
BssSI C'ACGA_G
Cuts at: 1568 3010 3368 1568
Size: 1442 358 2939
Fragments arranged by size:
2939 1442 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2116 2116
Size: 4739
BstBI TT'CG_AA
Cuts at: 3100 3100
Size: 4739
BstYI r'GATC_y
Cuts at: 1 192 198 743 828 1764 2231 2254 2589
Size: 191 6 545 85 936 467 23 335
Cuts at: 2589 2835 3847 3933 3945 4291 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 936 545 467 449 346 335 246
191 86 85 23 12 6
BtgI C'CryG_G
Cuts at: 107 1184 107
Size: 1077 3662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1164 2689 2870 3033 1164
Size: 1525 181 163 2870
Fragments arranged by size:
2870 1525 181 163
BtsI GCAGTG_nn'
Cuts at: 1119 1982 1119
Size: 863 3876
DraI TTT'AAA
Cuts at: 1002 1539 1738 1002
Size: 537 199 4003
Fragments arranged by size:
4003 537 199
DrdI GACnn_nn'nnGTC
Cuts at: 2445 3303 2445
Size: 858 3881
EaeI y'GGCC_r
Cuts at: 2324 2498 2889 2916 4261 2324
Size: 174 391 27 1345 2802
Fragments arranged by size:
2802 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2324 2324
Size: 4739
EarI CTCTTCn'nnn_
Cuts at: 2762 2972 2762
Size: 210 4529
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2947 3257 3403 4549 2947
Size: 310 146 1146 3137
Fragments arranged by size:
3137 1146 310 146
EcoICRI GAG'CTC
Cuts at: 491 1751 491
Size: 1260 3479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 590 602 683 1444 1580 2564 2996 3743
Size: 330 12 81 761 136 984 432 747
Cuts at: 3743 4011 260
Size: 268 988
Fragments arranged by size:
988 984 761 747 432 330 268 136
81 12
EcoO109I rG'GnC_Cy
Cuts at: 1189 1190 1239 1455 1878 1189
Size: 1 49 216 423 4050
Fragments arranged by size:
4050 423 216 49 1
EcoRI G'AATT_C
Cuts at: 1743 1743
Size: 4739
EcoRV GAT'ATC
Cuts at: 117 117
Size: 4739
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4739
FspI TGC'GCA
Cuts at: 2520 2520
Size: 4739
HaeII r_GCGC'y
Cuts at: 2173 2421 3443 4422 2173
Size: 248 1022 979 2490
Fragments arranged by size:
2490 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 418 429 450 461 531 548 563 580 888
Size: 11 21 11 70 17 15 17 308
Cuts at: 888 920 1304 1336 1639 1671 2581 2611 2613
Size: 32 384 32 303 32 910 30 2
Cuts at: 2613 2643 418
Size: 30 2514
Fragments arranged by size:
2514 910 384 308 303 70 32 32
32 30 30 21 17 17 15 11
11 2
HincII GTy'rAC
Cuts at: 1778 1778
Size: 4739
KasI G'GCGC_C
Cuts at: 2169 2417 2169
Size: 248 4491
KpnI G_GTAC'C
Cuts at: 679 1759 679
Size: 1080 3659
MluI A'CGCG_T
Cuts at: 4172 4172
Size: 4739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 534 1414 3410 3594 534
Size: 880 1996 184 1679
Fragments arranged by size:
1996 1679 880 184
MscI TGG'CCA
Cuts at: 2500 2500
Size: 4739
MslI CAynn'nnrTG
Cuts at: 233 1183 2728 2855 233
Size: 950 1545 127 2117
Fragments arranged by size:
2117 1545 950 127
MspA1I CmG'CkG
Cuts at: 1013 1094 1850 2164 2524 3537 3782 1013
Size: 81 756 314 360 1013 245 1970
Fragments arranged by size:
1970 1013 756 360 314 245 81
NaeI GCC'GGC
Cuts at: 1477 2920 1477
Size: 1443 3296
NarI GG'CG_CC
Cuts at: 2170 2418 2170
Size: 248 4491
NcoI C'CATG_G
Cuts at: 107 1184 107
Size: 1077 3662
NgoMIV G'CCGG_C
Cuts at: 1475 2918 1475
Size: 1443 3296
NspI r_CATG'y
Cuts at: 226 1792 2292 3199 226
Size: 1566 500 907 1766
Fragments arranged by size:
1766 1566 907 500
PasI CC'CwG_GG
Cuts at: 535 670 535
Size: 135 4604
PciI A'CATG_T
Cuts at: 3195 3195
Size: 4739
PmeI GTTT'AAAC
Cuts at: 1738 1738
Size: 4739
PmlI CAC'GTG
Cuts at: 363 363
Size: 4739
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 3878
Size: 32 4707
PpuMI rG'GwC_Cy
Cuts at: 1239 1455 1239
Size: 216 4523
PsiI TTA'TAA
Cuts at: 482 482
Size: 4739
PspOMI G'GGCC_C
Cuts at: 1189 1189
Size: 4739
PstI C_TGCA'G
Cuts at: 1786 2471 1786
Size: 685 4054
PvuI CG_AT'CG
Cuts at: 104 436 104
Size: 332 4407
PvuII CAG'CTG
Cuts at: 1013 1094 2164 2524 1013
Size: 81 1070 360 3228
Fragments arranged by size:
3228 1070 360 81
RsrII CG'GwC_CG
Cuts at: 2934 2934
Size: 4739
SacI G_AGCT'C
Cuts at: 493 1753 493
Size: 1260 3479
SalI G'TCGA_C
Cuts at: 1776 1776
Size: 4739
SapI GCTCTTCn'nnn_
Cuts at: 2762 2972 2762
Size: 210 4529
SbfI CC_TGCA'GG
Cuts at: 1786 1786
Size: 4739
ScaI AGT'ACT
Cuts at: 4256 4256
Size: 4739
SexAI A'CCwGG_T
Cuts at: 474 474
Size: 4739
SfcI C'TryA_G
Cuts at: 33 1153 1480 1782 2467 3460 3651 33
Size: 1120 327 302 685 993 191 1121
Fragments arranged by size:
1121 1120 993 685 327 302 191
SfoI GGC'GCC
Cuts at: 2171 2419 2171
Size: 248 4491
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4739
SmaI CCC'GGG
Cuts at: 1761 1761
Size: 4739
SmlI C'TyrA_G
Cuts at: 1894 2809 3301 3563 1894
Size: 915 492 262 3070
Fragments arranged by size:
3070 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4739
SphI G_CATG'C
Cuts at: 1792 2292 1792
Size: 500 4239
SspI AAT'ATT
Cuts at: 1035 1035
Size: 4739
StyI C'CwwG_G
Cuts at: 107 787 1184 1520 1873 2227 107
Size: 680 397 336 353 354 2619
Fragments arranged by size:
2619 680 397 354 353 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1308 2322 2455 2914 3123 1308
Size: 1014 133 459 209 2924
Fragments arranged by size:
2924 1014 459 209 133
TatI w'GTAC_w
Cuts at: 225 1173 4254 225
Size: 948 3081 710
Fragments arranged by size:
3081 948 710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4256 4288 4256
Size: 32 4707
Tth111I GACn'n_nGTC
Cuts at: 2536 2536
Size: 4739
XbaI T'CTAG_A
Cuts at: 1770 1770
Size: 4739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 769 1270 2234 769
Size: 501 964 3274
Fragments arranged by size:
3274 964 501
XmaI C'CCGG_G
Cuts at: 1759 1759
Size: 4739
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PshAI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI