Restriction map for
pF1KB9879
(Circular) (Six-base) MAPSORT of: pF1KB9879.seq Check: 5569 from: 1 to: 4865
pF1KB9879 4865 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1903 1903
Size: 4865
Acc65I G'GTAC_C
Cuts at: 1881 1881
Size: 4865
AclI AA'CG_TT
Cuts at: 4705 4705
Size: 4865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 943 1405 2690 3122 3869 4137 174
Size: 769 462 1285 432 747 268 902
Fragments arranged by size:
1285 902 769 747 462 432 268
AflII C'TTAA_G
Cuts at: 322 322
Size: 4865
AflIII A'CryG_T
Cuts at: 2217 3321 4298 2217
Size: 1104 977 2784
Fragments arranged by size:
2784 1104 977
AgeI A'CCGG_T
Cuts at: 3253 3253
Size: 4865
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 262 301 734 262
Size: 39 433 4393
Fragments arranged by size:
4393 433 39
AlwNI CAG_nnn'CTG
Cuts at: 622 807 2170 3737 622
Size: 185 1363 1567 1750
Fragments arranged by size:
1750 1567 1363 185
ApaI G_GGCC'C
Cuts at: 314 371 1605 1726 314
Size: 57 1234 121 3453
Fragments arranged by size:
3453 1234 121 57
ApaLI G'TGCA_C
Cuts at: 3635 3635
Size: 4865
ApoI r'AATT_y
Cuts at: 1869 4218 4474 1869
Size: 2349 256 2260
Fragments arranged by size:
2349 2260 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4865
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4865
AvaI C'yCGr_G
Cuts at: 292 307 1261 1700 1746 1885 292
Size: 15 954 439 46 139 3272
Fragments arranged by size:
3272 954 439 139 46 15
BamHI G'GATC_C
Cuts at: 1890 1890
Size: 4865
BanI G'GyrC_C
Cuts at: 651 779 1230 1326 1459 1668 1881 2295 2543
Size: 128 451 96 133 209 213 414 248
Cuts at: 2543 2578 651
Size: 35 2938
Fragments arranged by size:
2938 451 414 248 213 209 133 128
96 35
BanII G_rGCy'C
Cuts at: 314 371 1215 1605 1726 1879 2909 314
Size: 57 844 390 121 153 1030 2270
Fragments arranged by size:
2270 1030 844 390 153 121 57
BbeI G_GCGC'C
Cuts at: 783 1234 1463 2299 2547 783
Size: 451 229 836 248 3101
Fragments arranged by size:
3101 836 451 248 229
BbsI GAAGACnn'nnnn_
Cuts at: 1646 1646
Size: 4865
BbvCI CC'TCA_GC
Cuts at: 548 548
Size: 4865
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 874 908 874
Size: 34 4831
BciVI GTATCCnnnnn_n'
Cuts at: 901 2763 3524 4865 901
Size: 1862 761 1341 901
Fragments arranged by size:
1862 1341 901 761
BclI T'GATC_A
Cuts at: 2385 2385
Size: 4865
BglI GCCn_nnn'nGGC
Cuts at: 600 1186 600
Size: 586 4279
BglII A'GATC_T
Cuts at: 2380 4417 2380
Size: 2037 2828
BlpI GC'TnA_GC
Cuts at: 1977 1977
Size: 4865
Bme1580I G_kGCm'C
Cuts at: 314 371 1367 1605 1663 1673 1726 1765 1787
Size: 57 996 238 58 10 53 39 22
Cuts at: 1787 2490 2583 3639 314
Size: 703 93 1056 1540
Fragments arranged by size:
1540 1056 996 703 238 93 58 57
53 39 22 10
BmrI ACTGGGnnnn_n'
Cuts at: 1329 1595 2077 2158 2493 4637 1329
Size: 266 482 81 335 2144 1557
Fragments arranged by size:
2144 1557 482 335 266 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 3232 318
Size: 2914 1951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 887 1847 511
Size: 376 960 3529
Fragments arranged by size:
3529 960 376
Bpu10I CC'TnA_GC
Cuts at: 548 548
Size: 4865
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 681 2041 2920 3412 3710 256
Size: 425 1360 879 492 298 1411
Fragments arranged by size:
1411 1360 879 492 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 1345 4569 1345
Size: 3224 1641
BsaAI yAC'GTr
Cuts at: 285 2218 285
Size: 1933 2932
BsaBI GATnn'nnATC
Cuts at: 6 2379 4416 6
Size: 2373 2037 455
Fragments arranged by size:
2373 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 124 780 971 1118 1231 1460 2296 2544
Size: 12 656 191 147 113 229 836 248
Cuts at: 2544 3246 4742 112
Size: 702 1496 235
Fragments arranged by size:
1496 836 702 656 248 235 229 191
147 113 12
BsaWI w'CCGG_w
Cuts at: 2054 2575 3253 3527 3674 4085 2054
Size: 521 678 274 147 411 2834
Fragments arranged by size:
2834 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 1315 1467 1767 550
Size: 765 152 300 3648
Fragments arranged by size:
3648 765 300 152
BseYI C'CCAG_C
Cuts at: 371 392 398 525 1095 1160 1282 1487 1499
Size: 21 6 127 570 65 122 205 12
Cuts at: 1499 2290 3048 3625 4121 371
Size: 791 758 577 496 1115
Fragments arranged by size:
1115 791 758 577 570 496 205 127
122 65 21 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 1472 1644 139
Size: 1333 172 3360
Fragments arranged by size:
3360 1333 172
BsiEI CG_ry'CG
Cuts at: 104 1730 2407 2453 3661 104
Size: 1626 677 46 1208 1308
Fragments arranged by size:
1626 1308 1208 677 46
BsiHKAI G_wGCw'C
Cuts at: 290 1879 2657 2847 3639 290
Size: 1589 778 190 792 1516
Fragments arranged by size:
1589 1516 792 778 190
BsmI GAATG_Cn'
Cuts at: 723 723
Size: 4865
Bsp1286I G_dGCh'C
Cuts at: 290 314 371 1215 1367 1605 1663 1673 1726
Size: 24 57 844 152 238 58 10 53
Cuts at: 1726 1765 1787 1879 2490 2583 2657 2847 2909
Size: 39 22 92 611 93 74 190 62
Cuts at: 2909 3639 290
Size: 730 1516
Fragments arranged by size:
1516 844 730 611 238 190 152 93
92 74 62 58 57 53 39 24
22 10
BspEI T'CCGG_A
Cuts at: 4085 4085
Size: 4865
BspHI T'CATG_A
Cuts at: 4041 4041
Size: 4865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 164 1915 2129 2431 2812 164
Size: 1751 214 302 381 2217
Fragments arranged by size:
2217 1751 381 302 214
BsrBI CCG'CTC
Cuts at: 50 302 1632 1751 3157 3211 4695 4860 50
Size: 252 1330 119 1406 54 1484 165 55
Fragments arranged by size:
1484 1406 1330 252 165 119 55 54
BsrDI GCAATG_nn'
Cuts at: 963 1373 2777 963
Size: 410 1404 3051
Fragments arranged by size:
3051 1404 410
BsrFI r'CCGG_y
Cuts at: 412 967 1067 1176 2863 3044 3253 412
Size: 555 100 109 1687 181 209 2024
Fragments arranged by size:
2024 1687 555 209 181 109 100
BssSI C'ACGA_G
Cuts at: 3136 3494 3136
Size: 358 4507
BstAPI GCAn_nnn'nTGC
Cuts at: 2242 2242
Size: 4865
BstBI TT'CG_AA
Cuts at: 3226 3226
Size: 4865
BstXI CCAn_nnnn'nTGG
Cuts at: 345 525 345
Size: 180 4685
BstYI r'GATC_y
Cuts at: 1 1890 2357 2380 2715 2961 3973 4059 4071
Size: 1889 467 23 335 246 1012 86 12
Cuts at: 4071 4417 1
Size: 346 449
Fragments arranged by size:
1889 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 169 176 542 1145 1481 107
Size: 62 7 366 603 336 3491
Fragments arranged by size:
3491 603 366 336 62 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1489 2815 2996 3159 1489
Size: 1326 181 163 3195
Fragments arranged by size:
3195 1326 181 163
BtsI GCAGTG_nn'
Cuts at: 623 797 986 2108 623
Size: 174 189 1122 3380
Fragments arranged by size:
3380 1122 189 174
ClaI AT'CG_AT
Cuts at: 254 254
Size: 4865
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1803 1838 1803
Size: 35 4830
DraI TTT'AAA
Cuts at: 1864 1864
Size: 4865
DraIII CAC_nnn'GTG
Cuts at: 1323 1323
Size: 4865
DrdI GACnn_nn'nnGTC
Cuts at: 2571 3429 2571
Size: 858 4007
EaeI y'GGCC_r
Cuts at: 332 515 1394 1727 2450 2624 3015 3042 4387
Size: 183 879 333 723 174 391 27 1345
Cuts at: 4387 332
Size: 810
Fragments arranged by size:
1345 879 810 723 391 333 183 174
27
EagI C'GGCC_G
Cuts at: 1727 2450 1727
Size: 723 4142
EarI CTCTTCn'nnn_
Cuts at: 685 934 2888 3098 685
Size: 249 1954 210 2452
Fragments arranged by size:
2452 1954 249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 807 1445 3073 3383 3529 4675 807
Size: 638 1628 310 146 1146 997
Fragments arranged by size:
1628 1146 997 638 310 146
EcoICRI GAG'CTC
Cuts at: 1877 1877
Size: 4865
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 511 887 943 1405 1847 2690 3122 3869
Size: 337 376 56 462 442 843 432 747
Cuts at: 3869 4137 174
Size: 268 902
Fragments arranged by size:
902 843 747 462 442 432 376 337
268 56
EcoNI CCTnn'n_nnAGG
Cuts at: 1430 1430
Size: 4865
EcoO109I rG'GnC_Cy
Cuts at: 311 342 367 368 506 563 1152 1422 1441
Size: 31 25 1 138 57 589 270 19
Cuts at: 1441 1601 1602 1722 1854 2004 311
Size: 160 1 120 132 150 3172
Fragments arranged by size:
3172 589 270 160 150 138 132 120
57 31 25 19 1 1
EcoRI G'AATT_C
Cuts at: 1869 1869
Size: 4865
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1704 2884 1704
Size: 1180 3685
FspI TGC'GCA
Cuts at: 2646 2646
Size: 4865
HaeII r_GCGC'y
Cuts at: 783 814 1234 1463 2299 2547 3569 4548 783
Size: 31 420 229 836 248 1022 979 1100
Fragments arranged by size:
1100 1022 979 836 420 248 229 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2707 2737 2739 2769 2707
Size: 30 2 30 4803
Fragments arranged by size:
4803 30 30 2
HincII GTy'rAC
Cuts at: 1904 1904
Size: 4865
KasI G'GCGC_C
Cuts at: 779 1230 1459 2295 2543 779
Size: 451 229 836 248 3101
Fragments arranged by size:
3101 836 451 248 229
KpnI G_GTAC'C
Cuts at: 1885 1885
Size: 4865
MluI A'CGCG_T
Cuts at: 4298 4298
Size: 4865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3536 3720 3536
Size: 184 4681
MscI TGG'CCA
Cuts at: 517 2626 517
Size: 2109 2756
MslI CAynn'nnrTG
Cuts at: 2854 2981 2854
Size: 127 4738
MspA1I CmG'CkG
Cuts at: 375 613 1095 1147 1348 1549 1568 1580 1767
Size: 238 482 52 201 201 19 12 187
Cuts at: 1767 1976 2290 2650 3663 3908 375
Size: 209 314 360 1013 245 1332
Fragments arranged by size:
1332 1013 482 360 314 245 238 209
201 201 187 52 19 12
NaeI GCC'GGC
Cuts at: 969 1069 1178 3046 969
Size: 100 109 1868 2788
Fragments arranged by size:
2788 1868 109 100
NarI GG'CG_CC
Cuts at: 780 1231 1460 2296 2544 780
Size: 451 229 836 248 3101
Fragments arranged by size:
3101 836 451 248 229
NcoI C'CATG_G
Cuts at: 107 176 107
Size: 69 4796
NgoMIV G'CCGG_C
Cuts at: 967 1067 1176 3044 967
Size: 100 109 1868 2788
Fragments arranged by size:
2788 1868 109 100
NspI r_CATG'y
Cuts at: 1918 2418 3325 1918
Size: 500 907 3458
Fragments arranged by size:
3458 907 500
PasI CC'CwG_GG
Cuts at: 598 1404 1597 1787 598
Size: 806 193 190 3676
Fragments arranged by size:
3676 806 193 190
PciI A'CATG_T
Cuts at: 3321 3321
Size: 4865
PflMI CCAn_nnn'nTGG
Cuts at: 182 182
Size: 4865
PfoI T'CCnGG_A
Cuts at: 903 1219 903
Size: 316 4549
PmeI GTTT'AAAC
Cuts at: 1864 1864
Size: 4865
PmlI CAC'GTG
Cuts at: 285 285
Size: 4865
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 4004
Size: 32 4833
PpuMI rG'GwC_Cy
Cuts at: 342 506 1422 1854 342
Size: 164 916 432 3353
Fragments arranged by size:
3353 916 432 164
PspOMI G'GGCC_C
Cuts at: 310 367 1601 1722 310
Size: 57 1234 121 3453
Fragments arranged by size:
3453 1234 121 57
PstI C_TGCA'G
Cuts at: 618 1473 1912 2597 618
Size: 855 439 685 2886
Fragments arranged by size:
2886 855 685 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4865
PvuII CAG'CTG
Cuts at: 375 613 1095 2290 2650 375
Size: 238 482 1195 360 2590
Fragments arranged by size:
2590 1195 482 360 238
RsrII CG'GwC_CG
Cuts at: 234 3060 234
Size: 2826 2039
SacI G_AGCT'C
Cuts at: 1879 1879
Size: 4865
SacII CC_GC'GG
Cuts at: 1148 1148
Size: 4865
SalI G'TCGA_C
Cuts at: 1902 1902
Size: 4865
SanDI GG'GwC_CC
Cuts at: 506 1854 506
Size: 1348 3517
SapI GCTCTTCn'nnn_
Cuts at: 934 2888 3098 934
Size: 1954 210 2701
Fragments arranged by size:
2701 1954 210
SbfI CC_TGCA'GG
Cuts at: 1912 1912
Size: 4865
ScaI AGT'ACT
Cuts at: 4382 4382
Size: 4865
SfcI C'TryA_G
Cuts at: 33 614 1469 1908 2593 3586 3777 33
Size: 581 855 439 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 581 439 191
SfoI GGC'GCC
Cuts at: 781 1232 1461 2297 2545 781
Size: 451 229 836 248 3101
Fragments arranged by size:
3101 836 451 248 229
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4865
SgrAI Cr'CCGG_yG
Cuts at: 967 967
Size: 4865
SmaI CCC'GGG
Cuts at: 309 1702 1887 309
Size: 1393 185 3287
Fragments arranged by size:
3287 1393 185
SmlI C'TyrA_G
Cuts at: 271 322 660 2020 2935 3427 3689 271
Size: 51 338 1360 915 492 262 1447
Fragments arranged by size:
1447 1360 915 492 338 262 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4865
SphI G_CATG'C
Cuts at: 1918 2418 1918
Size: 500 4365
SrfI GCCC'GGGC
Cuts at: 309 309
Size: 4865
StuI AGG'CCT
Cuts at: 641 641
Size: 4865
StyI C'CwwG_G
Cuts at: 107 176 338 1418 1999 2353 107
Size: 69 162 1080 581 354 2619
Fragments arranged by size:
2619 1080 581 354 162 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1311 1431 1660 2448 2581 3040 3249 1311
Size: 120 229 788 133 459 209 2927
Fragments arranged by size:
2927 788 459 229 209 133 120
TatI w'GTAC_w
Cuts at: 494 1739 4380 494
Size: 1245 2641 979
Fragments arranged by size:
2641 1245 979
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4382 4414 4382
Size: 32 4833
Tth111I GACn'n_nGTC
Cuts at: 1117 2662 1117
Size: 1545 3320
XbaI T'CTAG_A
Cuts at: 1896 1896
Size: 4865
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 2360 342
Size: 2018 2847
XmaI C'CCGG_G
Cuts at: 307 1700 1885 307
Size: 1393 185 3287
Fragments arranged by size:
3287 1393 185
XmnI GAAnn'nnTTC
Cuts at: 928 928
Size: 4865
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspXI PsrI SexAI SfiI SnaBI SspI
SwaI XhoI ZraI