Restriction map for
pF1KB9885
(Circular) (Six-base) MAPSORT of: pF1KB9885.seq Check: 5258 from: 1 to: 5072
pF1KB9885 5072 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1134 1134
Size: 5072
AccI GT'mk_AC
Cuts at: 1290 1688 2110 1290
Size: 398 422 4252
Fragments arranged by size:
4252 422 398
Acc65I G'GTAC_C
Cuts at: 2088 2088
Size: 5072
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 761 2897 3329 4076 4344 622
Size: 139 2136 432 747 268 1350
Fragments arranged by size:
2136 1350 747 432 268 139
AflII C'TTAA_G
Cuts at: 949 949
Size: 5072
AflIII A'CryG_T
Cuts at: 2424 3528 4505 2424
Size: 1104 977 2991
Fragments arranged by size:
2991 1104 977
AgeI A'CCGG_T
Cuts at: 3460 3460
Size: 5072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1434 1434
Size: 5072
AlwNI CAG_nnn'CTG
Cuts at: 249 1048 1428 1998 2377 3944 249
Size: 799 380 570 379 1567 1377
Fragments arranged by size:
1567 1377 799 570 380 379
ApaLI G'TGCA_C
Cuts at: 3842 3842
Size: 5072
ApoI r'AATT_y
Cuts at: 1657 1901 2076 4425 4681 1657
Size: 244 175 2349 256 2048
Fragments arranged by size:
2349 2048 256 244 175
AseI AT'TA_AT
Cuts at: 20 20
Size: 5072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 917 2092 917
Size: 1175 3897
AvrII C'CTAG_G
Cuts at: 1114 1114
Size: 5072
BamHI G'GATC_C
Cuts at: 2097 2097
Size: 5072
BanI G'GyrC_C
Cuts at: 1681 2088 2502 2750 2785 1681
Size: 407 414 248 35 3968
Fragments arranged by size:
3968 414 407 248 35
BanII G_rGCy'C
Cuts at: 380 473 902 926 947 1093 2086 3116 380
Size: 93 429 24 21 146 993 1030 2336
Fragments arranged by size:
2336 1030 993 429 146 93 24 21
BbeI G_GCGC'C
Cuts at: 2506 2754 2506
Size: 248 4824
BbvCI CC'TCA_GC
Cuts at: 533 1676 533
Size: 1143 3929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1774 1808 1774
Size: 34 5038
BciVI GTATCCnnnnn_n'
Cuts at: 2970 3731 5072 2970
Size: 761 1341 2970
Fragments arranged by size:
2970 1341 761
BclI T'GATC_A
Cuts at: 2592 2592
Size: 5072
BglI GCCn_nnn'nGGC
Cuts at: 1036 1343 1036
Size: 307 4765
BglII A'GATC_T
Cuts at: 1380 2030 2587 4624 1380
Size: 650 557 2037 1828
Fragments arranged by size:
2037 1828 650 557
BlpI GC'TnA_GC
Cuts at: 379 1363 1513 2184 379
Size: 984 150 671 3267
Fragments arranged by size:
3267 984 671 150
Bme1580I G_kGCm'C
Cuts at: 1684 2697 2790 3846 1684
Size: 1013 93 1056 2910
Fragments arranged by size:
2910 1056 1013 93
BmrI ACTGGGnnnn_n'
Cuts at: 1334 2059 2284 2365 2700 4844 1334
Size: 725 225 81 335 2144 1562
Fragments arranged by size:
2144 1562 725 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1170 3439 1170
Size: 2269 2803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 646 889 1193 1738 1765 271
Size: 375 243 304 545 27 3578
Fragments arranged by size:
3578 545 375 304 243 27
Bpu10I CC'TnA_GC
Cuts at: 533 1676 1704 533
Size: 1143 28 3901
Fragments arranged by size:
3901 1143 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1447 2248 3127 3619 3917 1165
Size: 282 801 879 492 298 2320
Fragments arranged by size:
2320 879 801 492 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 4776 4776
Size: 5072
BsaAI yAC'GTr
Cuts at: 1242 2425 1242
Size: 1183 3889
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 423 543 2503 2751 3453 4949 423
Size: 120 1960 248 702 1496 546
Fragments arranged by size:
1960 1496 702 546 248 120
BsaWI w'CCGG_w
Cuts at: 1962 2261 2782 3460 3734 3881 4292 1962
Size: 299 521 678 274 147 411 2742
Fragments arranged by size:
2742 678 521 411 299 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 438 468 438
Size: 30 5042
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 550 694 787 886 901 904 1388 550
Size: 144 93 99 15 3 484 4234
Fragments arranged by size:
4234 484 144 99 93 15 3
BseYI C'CCAG_C
Cuts at: 329 373 1788 2497 3255 3832 4328 329
Size: 44 1415 709 758 577 496 1073
Fragments arranged by size:
1415 1073 758 709 577 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 826 202
Size: 624 4448
BsiEI CG_ry'CG
Cuts at: 104 2614 2660 3868 104
Size: 2510 46 1208 1308
Fragments arranged by size:
2510 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 902 926 947 1739 2086 2864 3054 3846 902
Size: 24 21 792 347 778 190 792 2128
Fragments arranged by size:
2128 792 792 778 347 190 24 21
BsmI GAATG_Cn'
Cuts at: 169 169
Size: 5072
Bsp1286I G_dGCh'C
Cuts at: 380 473 902 926 947 1093 1684 1739 2086
Size: 93 429 24 21 146 591 55 347
Cuts at: 2086 2697 2790 2864 3054 3116 3846 380
Size: 611 93 74 190 62 730 1606
Fragments arranged by size:
1606 730 611 591 429 347 190 146
93 93 74 62 55 24 21
BspEI T'CCGG_A
Cuts at: 4292 4292
Size: 5072
BspHI T'CATG_A
Cuts at: 4248 4248
Size: 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 794 1134 2122 2336 2638 3019 794
Size: 340 988 214 302 381 2847
Fragments arranged by size:
2847 988 381 340 302 214
BsrBI CCG'CTC
Cuts at: 50 540 3364 3418 4902 5067 50
Size: 490 2824 54 1484 165 55
Fragments arranged by size:
2824 1484 490 165 55 54
BsrDI GCAATG_nn'
Cuts at: 794 1418 1838 2019 2984 794
Size: 624 420 181 965 2882
Fragments arranged by size:
2882 965 624 420 181
BsrFI r'CCGG_y
Cuts at: 3070 3251 3460 3070
Size: 181 209 4682
Fragments arranged by size:
4682 209 181
BsrGI T'GTAC_A
Cuts at: 508 508
Size: 5072
BssSI C'ACGA_G
Cuts at: 1862 3343 3701 1862
Size: 1481 358 3233
Fragments arranged by size:
3233 1481 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2449 2449
Size: 5072
BstBI TT'CG_AA
Cuts at: 3433 3433
Size: 5072
BstXI CCAn_nnnn'nTGG
Cuts at: 177 670 177
Size: 493 4579
BstYI r'GATC_y
Cuts at: 1 1380 2030 2097 2564 2587 2922 3168 4180
Size: 1379 650 67 467 23 335 246 1012
Cuts at: 4180 4266 4278 4624 1
Size: 86 12 346 449
Fragments arranged by size:
1379 1012 650 467 449 346 335 246
86 67 23 12
BstZ17I GTA'TAC
Cuts at: 1291 1291
Size: 5072
Bsu36I CC'TnA_GG
Cuts at: 1145 1916 1985 1145
Size: 771 69 4232
Fragments arranged by size:
4232 771 69
BtgI C'CryG_G
Cuts at: 107 1031 1275 1522 107
Size: 924 244 247 3657
Fragments arranged by size:
3657 924 247 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3022 3203 3366 3022
Size: 181 163 4728
Fragments arranged by size:
4728 181 163
BtsI GCAGTG_nn'
Cuts at: 902 1174 1485 2315 902
Size: 272 311 830 3659
Fragments arranged by size:
3659 830 311 272
DraI TTT'AAA
Cuts at: 2071 2071
Size: 5072
DraIII CAC_nnn'GTG
Cuts at: 1491 1491
Size: 5072
DrdI GACnn_nn'nnGTC
Cuts at: 1958 2778 3636 1958
Size: 820 858 3394
Fragments arranged by size:
3394 858 820
EaeI y'GGCC_r
Cuts at: 225 263 501 836 1482 1548 2657 2831 3222
Size: 38 238 335 646 66 1109 174 391
Cuts at: 3222 3249 4594 225
Size: 27 1345 703
Fragments arranged by size:
1345 1109 703 646 391 335 238 174
66 38 27
EagI C'GGCC_G
Cuts at: 2657 2657
Size: 5072
EarI CTCTTCn'nnn_
Cuts at: 188 3095 3305 188
Size: 2907 210 1955
Fragments arranged by size:
2907 1955 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 618 1318 3280 3590 3736 4882 618
Size: 700 1962 310 146 1146 808
Fragments arranged by size:
1962 1146 808 700 310 146
EcoICRI GAG'CTC
Cuts at: 900 924 945 2084 900
Size: 24 21 1139 3888
Fragments arranged by size:
3888 1139 24 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 622 646 761 889 1193 1738 1765 2897
Size: 351 24 115 128 304 545 27 1132
Cuts at: 2897 3329 4076 4344 271
Size: 432 747 268 999
Fragments arranged by size:
1132 999 747 545 432 351 304 268
128 115 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1500 1500
Size: 5072
EcoO109I rG'GnC_Cy
Cuts at: 633 1118 1141 1774 2211 633
Size: 485 23 633 437 3494
Fragments arranged by size:
3494 633 485 437 23
EcoRI G'AATT_C
Cuts at: 2076 2076
Size: 5072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 630 3091 630
Size: 2461 2611
FspI TGC'GCA
Cuts at: 2853 2853
Size: 5072
HaeII r_GCGC'y
Cuts at: 2506 2754 3776 4755 2506
Size: 248 1022 979 2823
Fragments arranged by size:
2823 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 327 359 1594 1626 2914 2944 2946 2976 327
Size: 32 1235 32 1288 30 2 30 2423
Fragments arranged by size:
2423 1288 1235 32 32 30 30 2
HincII GTy'rAC
Cuts at: 566 1011 2111 566
Size: 445 1100 3527
Fragments arranged by size:
3527 1100 445
HindIII A'AGCT_T
Cuts at: 211 1365 1582 211
Size: 1154 217 3701
Fragments arranged by size:
3701 1154 217
HpaI GTT'AAC
Cuts at: 566 1011 566
Size: 445 4627
KasI G'GCGC_C
Cuts at: 2502 2750 2502
Size: 248 4824
KpnI G_GTAC'C
Cuts at: 2092 2092
Size: 5072
MluI A'CGCG_T
Cuts at: 4505 4505
Size: 5072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3743 3927 3743
Size: 184 4888
MscI TGG'CCA
Cuts at: 227 265 503 838 1484 1550 2833 227
Size: 38 238 335 646 66 1283 2466
Fragments arranged by size:
2466 1283 646 335 238 66 38
MslI CAynn'nnrTG
Cuts at: 175 1852 3061 3188 175
Size: 1677 1209 127 2059
Fragments arranged by size:
2059 1677 1209 127
MspA1I CmG'CkG
Cuts at: 201 287 333 373 532 600 880 1137 1512
Size: 86 46 40 159 68 280 257 375
Cuts at: 1512 1631 1788 1835 1995 2183 2497 2857 3870
Size: 119 157 47 160 188 314 360 1013
Cuts at: 3870 4115 201
Size: 245 1158
Fragments arranged by size:
1158 1013 375 360 314 280 257 245
188 160 159 157 119 86 68 47
46 40
NaeI GCC'GGC
Cuts at: 3253 3253
Size: 5072
NarI GG'CG_CC
Cuts at: 2503 2751 2503
Size: 248 4824
NcoI C'CATG_G
Cuts at: 107 1031 1522 107
Size: 924 491 3657
Fragments arranged by size:
3657 924 491
NgoMIV G'CCGG_C
Cuts at: 3251 3251
Size: 5072
NspI r_CATG'y
Cuts at: 1851 2125 2625 3532 1851
Size: 274 500 907 3391
Fragments arranged by size:
3391 907 500 274
PasI CC'CwG_GG
Cuts at: 1698 1698
Size: 5072
PciI A'CATG_T
Cuts at: 3528 3528
Size: 5072
PflMI CCAn_nnn'nTGG
Cuts at: 249 1491 249
Size: 1242 3830
PfoI T'CCnGG_A
Cuts at: 149 662 692 1209 149
Size: 513 30 517 4012
Fragments arranged by size:
4012 517 513 30
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 5040
PpuMI rG'GwC_Cy
Cuts at: 633 1141 633
Size: 508 4564
PshAI GACnn'nnGTC
Cuts at: 1889 1889
Size: 5072
PstI C_TGCA'G
Cuts at: 117 599 2119 2804 117
Size: 482 1520 685 2385
Fragments arranged by size:
2385 1520 685 482
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5072
PvuII CAG'CTG
Cuts at: 201 333 373 532 880 1512 1631 1788 1995
Size: 132 40 159 348 632 119 157 207
Cuts at: 1995 2497 2857 201
Size: 502 360 2416
Fragments arranged by size:
2416 632 502 360 348 207 159 157
132 119 40
RsrII CG'GwC_CG
Cuts at: 3267 3267
Size: 5072
SacI G_AGCT'C
Cuts at: 902 926 947 2086 902
Size: 24 21 1139 3888
Fragments arranged by size:
3888 1139 24 21
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SapI GCTCTTCn'nnn_
Cuts at: 188 3095 3305 188
Size: 2907 210 1955
Fragments arranged by size:
2907 1955 210
SbfI CC_TGCA'GG
Cuts at: 2119 2119
Size: 5072
ScaI AGT'ACT
Cuts at: 1262 4589 1262
Size: 3327 1745
SfcI C'TryA_G
Cuts at: 33 113 595 1769 1929 2034 2115 2800 3793
Size: 80 482 1174 160 105 81 685 993
Cuts at: 3793 3984 33
Size: 191 1121
Fragments arranged by size:
1174 1121 993 685 482 191 160 105
81 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 1036 1036
Size: 5072
SfoI GGC'GCC
Cuts at: 2504 2752 2504
Size: 248 4824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SmaI CCC'GGG
Cuts at: 2094 2094
Size: 5072
SmlI C'TyrA_G
Cuts at: 949 1180 1462 2227 3142 3634 3896 949
Size: 231 282 765 915 492 262 2125
Fragments arranged by size:
2125 915 765 492 282 262 231
SpeI A'CTAG_T
Cuts at: 67 1263 67
Size: 1196 3876
SphI G_CATG'C
Cuts at: 2125 2625 2125
Size: 500 4572
StuI AGG'CCT
Cuts at: 1709 1709
Size: 5072
StyI C'CwwG_G
Cuts at: 107 614 629 1031 1114 1371 1475 1522 2206
Size: 507 15 402 83 257 104 47 684
Cuts at: 2206 2560 107
Size: 354 2619
Fragments arranged by size:
2619 684 507 402 354 257 104 83
47 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 398 1711 2655 2788 3247 3456 398
Size: 1313 944 133 459 209 2014
Fragments arranged by size:
2014 1313 944 459 209 133
TatI w'GTAC_w
Cuts at: 508 1260 4587 508
Size: 752 3327 993
Fragments arranged by size:
3327 993 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1601 1633 4589 4621 1601
Size: 32 2956 32 2052
Fragments arranged by size:
2956 2052 32 32
Tth111I GACn'n_nGTC
Cuts at: 2869 2869
Size: 5072
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 496 762 1429 1747 2567 496
Size: 266 667 318 820 3001
Fragments arranged by size:
3001 820 667 318 266
XmaI C'CCGG_G
Cuts at: 2092 2092
Size: 5072
XmnI GAAnn'nnTTC
Cuts at: 721 2055 721
Size: 1334 3738
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmBI BssHII BstEII
ClaI CspCI EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI