Restriction map for
pF1KB9891
(Circular) (Six-base) MAPSORT of: pF1KB9891.seq Check: 2988 from: 1 to: 4514
pF1KB9891 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:32 ..
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1469 2339 2771 3124 3786 1273
Size: 196 870 432 353 662 2001
Fragments arranged by size:
2001 870 662 432 353 196
AflIII A'CryG_T
Cuts at: 480 1866 3666 3947 480
Size: 1386 1800 281 1047
Fragments arranged by size:
1800 1386 1047 281
AlwNI CAG_nnn'CTG
Cuts at: 919 1819 3257 919
Size: 900 1438 2176
Fragments arranged by size:
2176 1438 900
ApaLI G'TGCA_C
Cuts at: 1341 3352 1341
Size: 2011 2503
ApoI r'AATT_y
Cuts at: 356 1026 1232 1273 1518 3867 4123 356
Size: 670 206 41 245 2349 256 747
Fragments arranged by size:
2349 747 670 256 245 206 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 1534 1534
Size: 4514
BamHI G'GATC_C
Cuts at: 1477 1539 1477
Size: 62 4452
BanI G'GyrC_C
Cuts at: 1211 1530 1944 2192 2227 1211
Size: 319 414 248 35 3498
Fragments arranged by size:
3498 414 319 248 35
BanII G_rGCy'C
Cuts at: 1186 1528 2558 1186
Size: 342 1030 3142
Fragments arranged by size:
3142 1030 342
BbeI G_GCGC'C
Cuts at: 1948 2196 1948
Size: 248 4266
BciVI GTATCCnnnnn_n'
Cuts at: 390 468 2412 3468 4514 390
Size: 78 1944 1056 1046 390
Fragments arranged by size:
1944 1056 1046 390 78
BclI T'GATC_A
Cuts at: 2034 2034
Size: 4514
BfrBI ATG'CAT
Cuts at: 982 982
Size: 4514
BglII A'GATC_T
Cuts at: 1256 2029 4066 1256
Size: 773 2037 1704
Fragments arranged by size:
2037 1704 773
BlpI GC'TnA_GC
Cuts at: 1626 1626
Size: 4514
Bme1580I G_kGCm'C
Cuts at: 650 1345 2139 2232 3356 650
Size: 695 794 93 1124 1808
Fragments arranged by size:
1808 1124 794 695 93
BmrI ACTGGGnnnn_n'
Cuts at: 1726 1807 2142 4286 1726
Size: 81 335 2144 1954
Fragments arranged by size:
2144 1954 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 436 620 827 203
Size: 233 184 207 3890
Fragments arranged by size:
3890 233 207 184
Bpu10I CC'TnA_GC
Cuts at: 1044 1044
Size: 4514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 520 1482 1690 2569 3283 3581 310
Size: 210 962 208 879 714 298 1243
Fragments arranged by size:
1243 962 879 714 298 210 208
BsaI GGTCTCn'nnnn_
Cuts at: 440 4218 440
Size: 3778 736
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 1945 2193 2895 4391 1945
Size: 248 702 1496 2068
Fragments arranged by size:
2068 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1703 2224 2902 3313 3460 3734 1703
Size: 521 678 411 147 274 2483
Fragments arranged by size:
2483 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 756 786 825 855 1392 1422 756
Size: 30 39 30 537 30 3848
Fragments arranged by size:
3848 537 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 200 212 1124 200
Size: 12 912 3590
Fragments arranged by size:
3590 912 12
BseYI C'CCAG_C
Cuts at: 153 726 1939 2697 3362 3770 153
Size: 573 1213 758 665 408 897
Fragments arranged by size:
1213 897 758 665 573 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 1198
Size: 4514
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3332 104
Size: 1952 46 1230 1286
Fragments arranged by size:
1952 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1345 1528 2306 2496 3356 1345
Size: 183 778 190 860 2503
Fragments arranged by size:
2503 860 778 190 183
BsmBI CGTCTCn'nnnn_
Cuts at: 454 454
Size: 4514
Bsp1286I G_dGCh'C
Cuts at: 650 1186 1345 1528 2139 2232 2306 2496 2558
Size: 536 159 183 611 93 74 190 62
Cuts at: 2558 3356 650
Size: 798 1808
Fragments arranged by size:
1808 798 611 536 190 183 159 93
74 62
BspHI T'CATG_A
Cuts at: 528 2946 528
Size: 2418 2096
BspMI ACCTGCnnnn'nnnn_
Cuts at: 927 1564 1778 2080 2461 927
Size: 637 214 302 381 2980
Fragments arranged by size:
2980 637 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2806 2860 4344 4509 50
Size: 2756 54 1484 165 55
Fragments arranged by size:
2756 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2426 2426
Size: 4514
BsrFI r'CCGG_y
Cuts at: 2512 2693 2512
Size: 181 4333
BssSI C'ACGA_G
Cuts at: 2785 3493 2785
Size: 708 3806
BstAPI GCAn_nnn'nTGC
Cuts at: 1891 1891
Size: 4514
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstYI r'GATC_y
Cuts at: 1 662 1256 1477 1539 2006 2029 2364 2610
Size: 661 594 221 62 467 23 335 246
Cuts at: 2610 2916 2928 3014 4066 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 661 594 467 449 335 306 246
221 86 62 23 12
BtgI C'CryG_G
Cuts at: 1076 1076
Size: 4514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 245 1757 245
Size: 1512 3002
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3564 2220
Size: 1344 3170
EaeI y'GGCC_r
Cuts at: 2099 2273 2664 2691 4036 2099
Size: 174 391 27 1345 2577
Fragments arranged by size:
2577 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 139 1101 1289 2537 2747 139
Size: 962 188 1248 210 1906
Fragments arranged by size:
1906 1248 962 210 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 209 668 2722 3464 3610 4324 209
Size: 459 2054 742 146 714 399
Fragments arranged by size:
2054 742 714 459 399 146
EcoICRI GAG'CTC
Cuts at: 1526 1526
Size: 4514
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 436 620 827 1273 1469 2339 2771 3124
Size: 233 184 207 446 196 870 432 353
Cuts at: 3124 3786 203
Size: 662 931
Fragments arranged by size:
931 870 662 446 432 353 233 207
196 184
EcoO109I rG'GnC_Cy
Cuts at: 1653 1653
Size: 4514
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1427 2533 1427
Size: 1106 3408
FspI TGC'GCA
Cuts at: 2295 2295
Size: 4514
HaeII r_GCGC'y
Cuts at: 507 1948 2196 3426 4197 507
Size: 1441 248 1230 771 824
Fragments arranged by size:
1441 1230 824 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 823 855 2356 2386 2388 2418 823
Size: 32 1501 30 2 30 2919
Fragments arranged by size:
2919 1501 32 30 30 2
HincII GTy'rAC
Cuts at: 1065 1553 1065
Size: 488 4026
HpaI GTT'AAC
Cuts at: 1065 1065
Size: 4514
KasI G'GCGC_C
Cuts at: 1944 2192 1944
Size: 248 4266
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MfeI C'AATT_G
Cuts at: 542 1188 542
Size: 646 3868
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 945 1433 3273 3457 945
Size: 488 1840 184 2002
Fragments arranged by size:
2002 1840 488 184
MscI TGG'CCA
Cuts at: 2275 2275
Size: 4514
MslI CAynn'nnrTG
Cuts at: 927 1351 1433 2503 2630 927
Size: 424 82 1070 127 2811
Fragments arranged by size:
2811 1070 424 127 82
MspA1I CmG'CkG
Cuts at: 172 1625 1939 2299 3083 3328 172
Size: 1453 314 360 784 245 1358
Fragments arranged by size:
1453 1358 784 360 314 245
NaeI GCC'GGC
Cuts at: 2695 2695
Size: 4514
NarI GG'CG_CC
Cuts at: 1945 2193 1945
Size: 248 4266
NgoMIV G'CCGG_C
Cuts at: 2693 2693
Size: 4514
NsiI A_TGCA'T
Cuts at: 984 984
Size: 4514
NspI r_CATG'y
Cuts at: 484 1506 1567 2067 3670 484
Size: 1022 61 500 1603 1328
Fragments arranged by size:
1603 1328 1022 500 61
PciI A'CATG_T
Cuts at: 480 3666 480
Size: 3186 1328
PflMI CCAn_nnn'nTGG
Cuts at: 775 775
Size: 4514
PfoI T'CCnGG_A
Cuts at: 774 774
Size: 4514
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 4482
PstI C_TGCA'G
Cuts at: 1561 2246 1561
Size: 685 3829
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 172 1939 2299 172
Size: 1767 360 2387
Fragments arranged by size:
2387 1767 360
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1528 1528
Size: 4514
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 1289 2537 2747 1289
Size: 1248 210 3056
Fragments arranged by size:
3056 1248 210
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 1407 4031 1407
Size: 2624 1890
SexAI A'CCwGG_T
Cuts at: 430 430
Size: 4514
SfcI C'TryA_G
Cuts at: 33 1001 1444 1557 2242 3210 3401 33
Size: 968 443 113 685 968 191 1146
Fragments arranged by size:
1146 968 968 685 443 191 113
SfoI GGC'GCC
Cuts at: 1946 2194 1946
Size: 248 4266
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1536 1536
Size: 4514
SmlI C'TyrA_G
Cuts at: 289 535 1497 1669 2584 3298 3560 289
Size: 246 962 172 915 714 262 1243
Fragments arranged by size:
1243 962 915 714 262 246 172
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 1567 2067 1567
Size: 500 4014
StuI AGG'CCT
Cuts at: 407 407
Size: 4514
StyI C'CwwG_G
Cuts at: 1648 2002 1648
Size: 354 4160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 370 1405 4029 370
Size: 1035 2624 855
Fragments arranged by size:
2624 1035 855
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 208 240 825 857 1097 1129 4031 4063 208
Size: 32 585 32 240 32 2902 32 659
Fragments arranged by size:
2902 659 585 240 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2311 2311
Size: 4514
XbaI T'CTAG_A
Cuts at: 1545 1545
Size: 4514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2009 2009
Size: 4514
XmaI C'CCGG_G
Cuts at: 1534 1534
Size: 4514
XmnI GAAnn'nnTTC
Cuts at: 1258 1258
Size: 4514
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BglI BmgBI BmtI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII NcoI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI