Restriction map for
pF1KB9893
(Circular) (Six-base) MAPSORT of: pF1KB9893.seq Check: 3416 from: 1 to: 6989
pF1KB9893 6989 bp
With 182 enzymes: SgfI *
January 5, 2011 13:33 ..
AccI GT'mk_AC
Cuts at: 554 908 2781 2997 4027 554
Size: 354 1873 216 1030 3516
Fragments arranged by size:
3516 1873 1030 354 216
Acc65I G'GTAC_C
Cuts at: 2319 4005 2319
Size: 1686 5303
AclI AA'CG_TT
Cuts at: 2919 6829 2919
Size: 3910 3079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1332 1477 1524 1622 1668 2281 2345 3745 3802
Size: 145 47 98 46 613 64 1400 57
Cuts at: 3802 3949 4814 5246 5993 6261 1332
Size: 147 865 432 747 268 2060
Fragments arranged by size:
2060 1400 865 747 613 432 268 147
145 98 64 57 47 46
AflIII A'CryG_T
Cuts at: 365 4341 5445 6422 365
Size: 3976 1104 977 932
Fragments arranged by size:
3976 1104 977 932
AgeI A'CCGG_T
Cuts at: 5377 5377
Size: 6989
AhdI GACnn_n'nnGTC
Cuts at: 130 781 2378 130
Size: 651 1597 4741
Fragments arranged by size:
4741 1597 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3961 3961
Size: 6989
AlwNI CAG_nnn'CTG
Cuts at: 791 1455 3940 4294 5861 791
Size: 664 2485 354 1567 1919
Fragments arranged by size:
2485 1919 1567 664 354
ApaLI G'TGCA_C
Cuts at: 5759 5759
Size: 6989
ApoI r'AATT_y
Cuts at: 408 565 858 1171 1200 1732 2794 2876 3004
Size: 157 293 313 29 532 1062 82 128
Cuts at: 3004 3993 6342 6598 408
Size: 989 2349 256 799
Fragments arranged by size:
2349 1062 989 799 532 313 293 256
157 128 82 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 6989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6989
AvaI C'yCGr_G
Cuts at: 3041 4009 3041
Size: 968 6021
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 226 259 226
Size: 33 6956
BamHI G'GATC_C
Cuts at: 3878 4014 3878
Size: 136 6853
BanI G'GyrC_C
Cuts at: 2319 3385 3405 4005 4419 4667 4702 2319
Size: 1066 20 600 414 248 35 4606
Fragments arranged by size:
4606 1066 600 414 248 35 20
BanII G_rGCy'C
Cuts at: 220 2090 2476 3309 3481 4003 5033 220
Size: 1870 386 833 172 522 1030 2176
Fragments arranged by size:
2176 1870 1030 833 522 386 172
BbeI G_GCGC'C
Cuts at: 4423 4671 4423
Size: 248 6741
BbvCI CC'TCA_GC
Cuts at: 2927 3164 2927
Size: 237 6752
BciVI GTATCCnnnnn_n'
Cuts at: 4887 5648 6989 4887
Size: 761 1341 4887
Fragments arranged by size:
4887 1341 761
BclI T'GATC_A
Cuts at: 2417 4509 2417
Size: 2092 4897
BfrBI ATG'CAT
Cuts at: 3631 3631
Size: 6989
BglII A'GATC_T
Cuts at: 4504 6541 4504
Size: 2037 4952
BlpI GC'TnA_GC
Cuts at: 4101 4101
Size: 6989
Bme1580I G_kGCm'C
Cuts at: 3299 4614 4707 5763 3299
Size: 1315 93 1056 4525
Fragments arranged by size:
4525 1315 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1773 2201 3089 4201 4282 4617 6761 1773
Size: 428 888 1112 81 335 2144 2001
Fragments arranged by size:
2144 2001 1112 888 428 335 81
BmtI G_CTAG'C
Cuts at: 2701 2701
Size: 6989
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1770 5356 1770
Size: 3586 3403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 424 833 952 2093 2794 316
Size: 108 409 119 1141 701 4511
Fragments arranged by size:
4511 1141 701 409 119 108
Bpu10I CC'TnA_GC
Cuts at: 822 2283 2591 2927 3164 822
Size: 1461 308 336 237 4647
Fragments arranged by size:
4647 1461 336 308 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2184 4165 5044 5536 5834 1402
Size: 782 1981 879 492 298 2557
Fragments arranged by size:
2557 1981 879 782 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3038 3587 6693 3038
Size: 549 3106 3334
Fragments arranged by size:
3334 3106 549
BsaAI yAC'GTr
Cuts at: 366 4342 366
Size: 3976 3013
BsaBI GATnn'nnATC
Cuts at: 6 1937 4503 6540 6
Size: 1931 2566 2037 455
Fragments arranged by size:
2566 2037 1931 455
BsaHI Gr'CG_yC
Cuts at: 4420 4668 5370 6866 4420
Size: 248 702 1496 4543
Fragments arranged by size:
4543 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2450 3968 4178 4699 5377 5651 5798 6209 2450
Size: 1518 210 521 678 274 147 411 3230
Fragments arranged by size:
3230 1518 678 521 411 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 536 566 866 896 2321 2351 3278 3308 536
Size: 30 300 30 1425 30 927 30 4217
Fragments arranged by size:
4217 1425 927 300 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1795 3181 3500 3574 1795
Size: 1386 319 74 5210
Fragments arranged by size:
5210 1386 319 74
BseYI C'CCAG_C
Cuts at: 172 698 2457 4414 5172 5749 6245 172
Size: 526 1759 1957 758 577 496 916
Fragments arranged by size:
1957 1759 916 758 577 526 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 767 1953 2925 215
Size: 552 1186 972 4279
Fragments arranged by size:
4279 1186 972 552
BsiEI CG_ry'CG
Cuts at: 104 4531 4577 5785 104
Size: 4427 46 1208 1308
Fragments arranged by size:
4427 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 220 317 938 1849 2540 3814 4003 4781 4971
Size: 97 621 911 691 1274 189 778 190
Cuts at: 4971 5763 220
Size: 792 1446
Fragments arranged by size:
1446 1274 911 792 778 691 621 190
189 97
BsmI GAATG_Cn'
Cuts at: 967 967
Size: 6989
Bsp1286I G_dGCh'C
Cuts at: 220 317 938 1849 2090 2476 2540 3299 3309
Size: 97 621 911 241 386 64 759 10
Cuts at: 3309 3481 3814 4003 4614 4707 4781 4971 5033
Size: 172 333 189 611 93 74 190 62
Cuts at: 5033 5763 220
Size: 730 1446
Fragments arranged by size:
1446 911 759 730 621 611 386 333
241 190 189 172 97 93 74 64
62 10
BspEI T'CCGG_A
Cuts at: 6209 6209
Size: 6989
BspHI T'CATG_A
Cuts at: 1998 6165 1998
Size: 4167 2822
BspMI ACCTGCnnnn'nnnn_
Cuts at: 404 3842 4039 4253 4555 4936 404
Size: 3438 197 214 302 381 2457
Fragments arranged by size:
3438 2457 381 302 214 197
BsrBI CCG'CTC
Cuts at: 50 5281 5335 6819 6984 50
Size: 5231 54 1484 165 55
Fragments arranged by size:
5231 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 717 4901 717
Size: 4184 2805
BsrFI r'CCGG_y
Cuts at: 114 4987 5168 5377 114
Size: 4873 181 209 1726
Fragments arranged by size:
4873 1726 209 181
BsrGI T'GTAC_A
Cuts at: 1837 1837
Size: 6989
BssSI C'ACGA_G
Cuts at: 148 2533 3482 5260 5618 148
Size: 2385 949 1778 358 1519
Fragments arranged by size:
2385 1778 1519 949 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3346 4366 3346
Size: 1020 5969
BstBI TT'CG_AA
Cuts at: 5350 5350
Size: 6989
BstEII G'GTnAC_C
Cuts at: 1368 1368
Size: 6989
BstXI CCAn_nnnn'nTGG
Cuts at: 1764 2487 2709 2762 3705 1764
Size: 723 222 53 943 5048
Fragments arranged by size:
5048 943 723 222 53
BstYI r'GATC_y
Cuts at: 1 1697 3878 4014 4481 4504 4839 5085 6097
Size: 1696 2181 136 467 23 335 246 1012
Cuts at: 6097 6183 6195 6541 1
Size: 86 12 346 449
Fragments arranged by size:
2181 1696 1012 467 449 346 335 246
136 86 23 12
BstZ17I GTA'TAC
Cuts at: 2782 2782
Size: 6989
Bsu36I CC'TnA_GG
Cuts at: 830 3182 830
Size: 2352 4637
BtgI C'CryG_G
Cuts at: 616 1815 2017 2508 616
Size: 1199 202 491 5097
Fragments arranged by size:
5097 1199 491 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4939 5120 5283 4939
Size: 181 163 6645
Fragments arranged by size:
6645 181 163
BtsI GCAGTG_nn'
Cuts at: 4232 4232
Size: 6989
ClaI AT'CG_AT
Cuts at: 447 447
Size: 6989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 852 887 1856 1891 3444 3479 852
Size: 35 969 35 1553 35 4362
Fragments arranged by size:
4362 1553 969 35 35 35
DraI TTT'AAA
Cuts at: 470 1857 3988 470
Size: 1387 2131 3471
Fragments arranged by size:
3471 2131 1387
DraIII CAC_nnn'GTG
Cuts at: 3346 3346
Size: 6989
DrdI GACnn_nn'nnGTC
Cuts at: 639 4695 5553 639
Size: 4056 858 2075
Fragments arranged by size:
4056 2075 858
EaeI y'GGCC_r
Cuts at: 690 1327 4574 4748 5139 5166 6511 690
Size: 637 3247 174 391 27 1345 1168
Fragments arranged by size:
3247 1345 1168 637 391 174 27
EagI C'GGCC_G
Cuts at: 4574 4574
Size: 6989
EarI CTCTTCn'nnn_
Cuts at: 1377 1543 2095 2134 2257 3131 5012 5222 1377
Size: 166 552 39 123 874 1881 210 3144
Fragments arranged by size:
3144 1881 874 552 210 166 123 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3891 5197 5507 5653 6799 3891
Size: 1306 310 146 1146 4081
Fragments arranged by size:
4081 1306 1146 310 146
EcoICRI GAG'CTC
Cuts at: 218 4001 218
Size: 3783 3206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 316 424 833 952 1332 1477 1524 1622 1668
Size: 108 409 119 380 145 47 98 46
Cuts at: 1668 2093 2281 2345 2794 3745 3802 3949 4814
Size: 425 188 64 449 951 57 147 865
Cuts at: 4814 5246 5993 6261 316
Size: 432 747 268 1044
Fragments arranged by size:
1044 951 865 747 449 432 425 409
380 268 188 147 145 119 108 98
64 57 47 46
EcoO109I rG'GnC_Cy
Cuts at: 324 703 826 1233 1648 1825 4128 324
Size: 379 123 407 415 177 2303 3185
Fragments arranged by size:
3185 2303 415 407 379 177 123
EcoRI G'AATT_C
Cuts at: 2876 3993 2876
Size: 1117 5872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 5008 2251
Size: 2757 4232
FspI TGC'GCA
Cuts at: 256 4770 256
Size: 4514 2475
HaeII r_GCGC'y
Cuts at: 4423 4671 5693 6672 4423
Size: 248 1022 979 4740
Fragments arranged by size:
4740 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 169 201 767 799 1125 1157 1682
Size: 32 21 32 566 32 326 32 525
Cuts at: 1682 1714 2364 2396 4831 4861 4863 4893 116
Size: 32 650 32 2435 30 2 30 2212
Fragments arranged by size:
2435 2212 650 566 525 326 32 32
32 32 32 32 30 30 21 2
HincII GTy'rAC
Cuts at: 644 4028 644
Size: 3384 3605
HindIII A'AGCT_T
Cuts at: 1103 3088 1103
Size: 1985 5004
KasI G'GCGC_C
Cuts at: 4419 4667 4419
Size: 248 6741
KpnI G_GTAC'C
Cuts at: 2323 4009 2323
Size: 1686 5303
MfeI C'AATT_G
Cuts at: 1247 1247
Size: 6989
MluI A'CGCG_T
Cuts at: 6422 6422
Size: 6989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 940 2501 2905 2956 5660 5844 650
Size: 290 1561 404 51 2704 184 1795
Fragments arranged by size:
2704 1795 1561 404 290 184 51
MscI TGG'CCA
Cuts at: 1329 4750 1329
Size: 3421 3568
MslI CAynn'nnrTG
Cuts at: 1762 2807 4978 5105 1762
Size: 1045 2171 127 3646
Fragments arranged by size:
3646 2171 1045 127
MspA1I CmG'CkG
Cuts at: 2071 2487 4100 4414 4774 5787 6032 2071
Size: 416 1613 314 360 1013 245 3028
Fragments arranged by size:
3028 1613 1013 416 360 314 245
NaeI GCC'GGC
Cuts at: 116 5170 116
Size: 5054 1935
NarI GG'CG_CC
Cuts at: 4420 4668 4420
Size: 248 6741
NcoI C'CATG_G
Cuts at: 616 1815 2017 2508 616
Size: 1199 202 491 5097
Fragments arranged by size:
5097 1199 491 202
NdeI CA'TA_TG
Cuts at: 960 960
Size: 6989
NgoMIV G'CCGG_C
Cuts at: 114 5168 114
Size: 5054 1935
NheI G'CTAG_C
Cuts at: 2697 2697
Size: 6989
NsiI A_TGCA'T
Cuts at: 3633 3633
Size: 6989
NspI r_CATG'y
Cuts at: 881 1814 2050 4042 4542 5449 881
Size: 933 236 1992 500 907 2421
Fragments arranged by size:
2421 1992 933 907 500 236
PciI A'CATG_T
Cuts at: 5445 5445
Size: 6989
PflMI CCAn_nnn'nTGG
Cuts at: 593 1526 593
Size: 933 6056
PfoI T'CCnGG_A
Cuts at: 1568 1568
Size: 6989
PmeI GTTT'AAAC
Cuts at: 3988 3988
Size: 6989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6128 6160 6128
Size: 32 6957
PpuMI rG'GwC_Cy
Cuts at: 324 703 826 1233 1648 1825 324
Size: 379 123 407 415 177 5488
Fragments arranged by size:
5488 415 407 379 177 123
PshAI GACnn'nnGTC
Cuts at: 1664 1664
Size: 6989
PsiI TTA'TAA
Cuts at: 2306 3863 2306
Size: 1557 5432
PstI C_TGCA'G
Cuts at: 3839 4036 4721 3839
Size: 197 685 6107
Fragments arranged by size:
6107 685 197
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6989
PvuII CAG'CTG
Cuts at: 2071 2487 4414 4774 2071
Size: 416 1927 360 4286
Fragments arranged by size:
4286 1927 416 360
RsrII CG'GwC_CG
Cuts at: 5184 5184
Size: 6989
SacI G_AGCT'C
Cuts at: 220 4003 220
Size: 3783 3206
SalI G'TCGA_C
Cuts at: 4026 4026
Size: 6989
SanDI GG'GwC_CC
Cuts at: 703 1233 703
Size: 530 6459
SapI GCTCTTCn'nnn_
Cuts at: 2095 2134 5012 5222 2095
Size: 39 2878 210 3862
Fragments arranged by size:
3862 2878 210 39
SbfI CC_TGCA'GG
Cuts at: 3839 4036 3839
Size: 197 6792
ScaI AGT'ACT
Cuts at: 262 1388 3172 6506 262
Size: 1126 1784 3334 745
Fragments arranged by size:
3334 1784 1126 745
SexAI A'CCwGG_T
Cuts at: 1364 1364
Size: 6989
SfcI C'TryA_G
Cuts at: 33 474 1392 1441 3300 3835 4032 4717 5710
Size: 441 918 49 1859 535 197 685 993
Cuts at: 5710 5901 33
Size: 191 1121
Fragments arranged by size:
1859 1121 993 918 685 535 441 197
191 49
SfoI GGC'GCC
Cuts at: 4421 4669 4421
Size: 248 6741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6989
SmaI CCC'GGG
Cuts at: 4011 4011
Size: 6989
SmlI C'TyrA_G
Cuts at: 1417 2163 3041 4144 5059 5551 5813 1417
Size: 746 878 1103 915 492 262 2593
Fragments arranged by size:
2593 1103 915 878 746 492 262
SpeI A'CTAG_T
Cuts at: 67 1993 67
Size: 1926 5063
SphI G_CATG'C
Cuts at: 4042 4542 4042
Size: 500 6489
SspI AAT'ATT
Cuts at: 464 3856 464
Size: 3392 3597
StyI C'CwwG_G
Cuts at: 455 616 1815 1974 2017 2277 2384 2508 3647
Size: 161 1199 159 43 260 107 124 1139
Cuts at: 3647 3704 4123 4477 455
Size: 57 419 354 2967
Fragments arranged by size:
2967 1199 1139 419 354 260 161 159
124 107 57 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 1967 1987 2235 2449 2815 3448 3660 3839
Size: 1841 20 248 214 366 633 212 179
Cuts at: 3839 4572 4705 5164 5373 126
Size: 733 133 459 209 1742
Fragments arranged by size:
1841 1742 733 633 459 366 248 214
212 209 179 133 20
TatI w'GTAC_w
Cuts at: 260 685 953 1386 1594 1837 2729 3170 3842
Size: 425 268 433 208 243 892 441 672
Cuts at: 3842 6504 260
Size: 2662 745
Fragments arranged by size:
2662 892 745 672 441 433 425 268
243 208
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 2321 2353 2432 2464 3278 3310 3398
Size: 32 1560 32 79 32 814 32 88
Cuts at: 3398 3430 3616 3648 6506 6538 729
Size: 32 186 32 2858 32 1180
Fragments arranged by size:
2858 1560 1180 814 186 88 79 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1823 4786 1823
Size: 2963 4026
XbaI T'CTAG_A
Cuts at: 4020 4020
Size: 6989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 927 2391 4484 927
Size: 1464 2093 3432
Fragments arranged by size:
3432 2093 1464
XhoI C'TCGA_G
Cuts at: 3041 3041
Size: 6989
XmaI C'CCGG_G
Cuts at: 4009 4009
Size: 6989
XmnI GAAnn'nnTTC
Cuts at: 565 565
Size: 6989
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BbsI BcgI BglI BmgBI BsiWI BsmBI BssHII
EcoNI EcoRV FseI FspAI HpaI NotI NruI PacI
PasI PmlI PspOMI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI