Restriction map for
pF1KB9903
(Circular) (Six-base) MAPSORT of: pF1KB9903.seq Check: 67 from: 1 to: 3878
pF1KB9903 3878 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 916 916
Size: 3878
Acc65I G'GTAC_C
Cuts at: 894 894
Size: 3878
AclI AA'CG_TT
Cuts at: 3718 3718
Size: 3878
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 773 787 1703 2135 2882 3150 773
Size: 14 916 432 747 268 1501
Fragments arranged by size:
1501 916 747 432 268 14
AflIII A'CryG_T
Cuts at: 1230 2334 3311 1230
Size: 1104 977 1797
Fragments arranged by size:
1797 1104 977
AgeI A'CCGG_T
Cuts at: 2266 2266
Size: 3878
AlwNI CAG_nnn'CTG
Cuts at: 366 661 1183 2750 366
Size: 295 522 1567 1494
Fragments arranged by size:
1567 1494 522 295
ApaI G_GGCC'C
Cuts at: 821 821
Size: 3878
ApaLI G'TGCA_C
Cuts at: 608 2648 608
Size: 2040 1838
ApoI r'AATT_y
Cuts at: 197 882 3231 3487 197
Size: 685 2349 256 588
Fragments arranged by size:
2349 685 588 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3878
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
AvaI C'yCGr_G
Cuts at: 898 898
Size: 3878
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 447 480 447
Size: 33 3845
BamHI G'GATC_C
Cuts at: 903 903
Size: 3878
BanI G'GyrC_C
Cuts at: 894 1308 1556 1591 894
Size: 414 248 35 3181
Fragments arranged by size:
3181 414 248 35
BanII G_rGCy'C
Cuts at: 821 892 1922 821
Size: 71 1030 2777
Fragments arranged by size:
2777 1030 71
BbeI G_GCGC'C
Cuts at: 1312 1560 1312
Size: 248 3630
BbsI GAAGACnn'nnnn_
Cuts at: 306 857 306
Size: 551 3327
BciVI GTATCCnnnnn_n'
Cuts at: 475 1776 2537 3878 475
Size: 1301 761 1341 475
Fragments arranged by size:
1341 1301 761 475
BclI T'GATC_A
Cuts at: 357 1398 357
Size: 1041 2837
BglII A'GATC_T
Cuts at: 1393 3430 1393
Size: 2037 1841
BlpI GC'TnA_GC
Cuts at: 990 990
Size: 3878
Bme1580I G_kGCm'C
Cuts at: 612 821 1503 1596 2652 612
Size: 209 682 93 1056 1838
Fragments arranged by size:
1838 1056 682 209 93
BmrI ACTGGGnnnn_n'
Cuts at: 424 1090 1171 1506 3650 424
Size: 666 81 335 2144 652
Fragments arranged by size:
2144 666 652 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2245
Size: 3878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 683
Size: 3878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1933 2425 2723 1054
Size: 879 492 298 2209
Fragments arranged by size:
2209 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3582 3582
Size: 3878
BsaAI yAC'GTr
Cuts at: 1231 1231
Size: 3878
BsaBI GATnn'nnATC
Cuts at: 6 1392 3429 6
Size: 1386 2037 455
Fragments arranged by size:
2037 1386 455
BsaHI Gr'CG_yC
Cuts at: 1309 1557 2259 3755 1309
Size: 248 702 1496 1432
Fragments arranged by size:
1496 1432 702 248
BsaWI w'CCGG_w
Cuts at: 477 1067 1588 2266 2540 2687 3098 477
Size: 590 521 678 274 147 411 1257
Fragments arranged by size:
1257 678 590 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1303 2061 2638 3134 1303
Size: 758 577 496 2047
Fragments arranged by size:
2047 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 171 1420 1466 2674 104
Size: 67 1249 46 1208 1308
Fragments arranged by size:
1308 1249 1208 67 46
BsiHKAI G_wGCw'C
Cuts at: 612 892 1670 1860 2652 612
Size: 280 778 190 792 1838
Fragments arranged by size:
1838 792 778 280 190
BsmI GAATG_Cn'
Cuts at: 729 729
Size: 3878
Bsp1286I G_dGCh'C
Cuts at: 612 821 892 1503 1596 1670 1860 1922 2652
Size: 209 71 611 93 74 190 62 730
Cuts at: 2652 612
Size: 1838
Fragments arranged by size:
1838 730 611 209 190 93 74 71
62
BspEI T'CCGG_A
Cuts at: 3098 3098
Size: 3878
BspHI T'CATG_A
Cuts at: 3054 3054
Size: 3878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 928 1142 1444 1825 928
Size: 214 302 381 2981
Fragments arranged by size:
2981 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2170 2224 3708 3873 50
Size: 2120 54 1484 165 55
Fragments arranged by size:
2120 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1790 1790
Size: 3878
BsrFI r'CCGG_y
Cuts at: 1876 2057 2266 1876
Size: 181 209 3488
Fragments arranged by size:
3488 209 181
BssSI C'ACGA_G
Cuts at: 406 2149 2507 406
Size: 1743 358 1777
Fragments arranged by size:
1777 1743 358
BstAPI GCAn_nnn'nTGC
Cuts at: 616 1255 616
Size: 639 3239
BstBI TT'CG_AA
Cuts at: 2239 2239
Size: 3878
BstEII G'GTnAC_C
Cuts at: 480 480
Size: 3878
BstYI r'GATC_y
Cuts at: 1 791 903 1370 1393 1728 1974 2986 3072
Size: 790 112 467 23 335 246 1012 86
Cuts at: 3072 3084 3430 1
Size: 12 346 449
Fragments arranged by size:
1012 790 467 449 346 335 246 112
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3878
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1828 2009 2172 1828
Size: 181 163 3534
Fragments arranged by size:
3534 181 163
BtsI GCAGTG_nn'
Cuts at: 1121 1121
Size: 3878
DraI TTT'AAA
Cuts at: 877 877
Size: 3878
DrdI GACnn_nn'nnGTC
Cuts at: 1584 2442 1584
Size: 858 3020
EaeI y'GGCC_r
Cuts at: 1463 1637 2028 2055 3400 1463
Size: 174 391 27 1345 1941
Fragments arranged by size:
1941 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1463 1463
Size: 3878
EarI CTCTTCn'nnn_
Cuts at: 833 1901 2111 833
Size: 1068 210 2600
Fragments arranged by size:
2600 1068 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 139 2086 2396 2542 3688 139
Size: 1947 310 146 1146 329
Fragments arranged by size:
1947 1146 329 310 146
EcoICRI GAG'CTC
Cuts at: 890 890
Size: 3878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 683 773 787 1703 2135 2882 3150 683
Size: 90 14 916 432 747 268 1411
Fragments arranged by size:
1411 916 747 432 268 90 14
EcoO109I rG'GnC_Cy
Cuts at: 817 1017 817
Size: 200 3678
EcoRI G'AATT_C
Cuts at: 882 882
Size: 3878
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 662 1897 662
Size: 1235 2643
FspI TGC'GCA
Cuts at: 1659 1659
Size: 3878
HaeII r_GCGC'y
Cuts at: 1312 1560 2582 3561 1312
Size: 248 1022 979 1629
Fragments arranged by size:
1629 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1720 1750 1752 1782 1720
Size: 30 2 30 3816
Fragments arranged by size:
3816 30 30 2
HincII GTy'rAC
Cuts at: 917 917
Size: 3878
KasI G'GCGC_C
Cuts at: 1308 1556 1308
Size: 248 3630
KpnI G_GTAC'C
Cuts at: 898 898
Size: 3878
MluI A'CGCG_T
Cuts at: 3311 3311
Size: 3878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2549 2733 2549
Size: 184 3694
MscI TGG'CCA
Cuts at: 1639 1639
Size: 3878
MslI CAynn'nnrTG
Cuts at: 1867 1994 1867
Size: 127 3751
MspA1I CmG'CkG
Cuts at: 989 1303 1663 2676 2921 989
Size: 314 360 1013 245 1946
Fragments arranged by size:
1946 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 3878
NarI GG'CG_CC
Cuts at: 1309 1557 1309
Size: 248 3630
NcoI C'CATG_G
Cuts at: 107 107
Size: 3878
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 3878
NspI r_CATG'y
Cuts at: 931 1431 2338 931
Size: 500 907 2471
Fragments arranged by size:
2471 907 500
PciI A'CATG_T
Cuts at: 2334 2334
Size: 3878
PflMI CCAn_nnn'nTGG
Cuts at: 661 661
Size: 3878
PmeI GTTT'AAAC
Cuts at: 877 877
Size: 3878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 374 406 574 606 3017 3049 374
Size: 32 168 32 2411 32 1203
Fragments arranged by size:
2411 1203 168 32 32 32
PsiI TTA'TAA
Cuts at: 518 518
Size: 3878
PspOMI G'GGCC_C
Cuts at: 817 817
Size: 3878
PstI C_TGCA'G
Cuts at: 782 925 1610 782
Size: 143 685 3050
Fragments arranged by size:
3050 685 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3878
PvuII CAG'CTG
Cuts at: 1303 1663 1303
Size: 360 3518
RsrII CG'GwC_CG
Cuts at: 2073 2073
Size: 3878
SacI G_AGCT'C
Cuts at: 892 892
Size: 3878
SalI G'TCGA_C
Cuts at: 915 915
Size: 3878
SapI GCTCTTCn'nnn_
Cuts at: 1901 2111 1901
Size: 210 3668
SbfI CC_TGCA'GG
Cuts at: 925 925
Size: 3878
ScaI AGT'ACT
Cuts at: 539 3395 539
Size: 2856 1022
SfcI C'TryA_G
Cuts at: 33 778 921 1606 2599 2790 33
Size: 745 143 685 993 191 1121
Fragments arranged by size:
1121 993 745 685 191 143
SfoI GGC'GCC
Cuts at: 1310 1558 1310
Size: 248 3630
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3878
SmaI CCC'GGG
Cuts at: 900 900
Size: 3878
SmlI C'TyrA_G
Cuts at: 1033 1948 2440 2702 1033
Size: 915 492 262 2209
Fragments arranged by size:
2209 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3878
SphI G_CATG'C
Cuts at: 931 1431 931
Size: 500 3378
StyI C'CwwG_G
Cuts at: 107 1012 1366 107
Size: 905 354 2619
Fragments arranged by size:
2619 905 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 1461 1594 2053 2262 406
Size: 1055 133 459 209 2022
Fragments arranged by size:
2022 1055 459 209 133
TatI w'GTAC_w
Cuts at: 149 537 3393 149
Size: 388 2856 634
Fragments arranged by size:
2856 634 388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 3846
Tth111I GACn'n_nGTC
Cuts at: 856 1675 856
Size: 819 3059
XbaI T'CTAG_A
Cuts at: 909 909
Size: 3878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1373 1373
Size: 3878
XmaI C'CCGG_G
Cuts at: 898 898
Size: 3878
XmnI GAAnn'nnTTC
Cuts at: 386 589 386
Size: 203 3675
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI