Restriction map for
pF1KB9908
(Circular) (Six-base) MAPSORT of: pF1KB9908.seq Check: 8481 from: 1 to: 4880
pF1KB9908 4880 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1746 1746
Size: 4880
AccI GT'mk_AC
Cuts at: 1918 1918
Size: 4880
Acc65I G'GTAC_C
Cuts at: 1896 1896
Size: 4880
AclI AA'CG_TT
Cuts at: 4720 4720
Size: 4880
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 648 1063 1758 2705 3137 3884 4152 648
Size: 415 695 947 432 747 268 1376
Fragments arranged by size:
1376 947 747 695 432 415 268
AfeI AGC'GCT
Cuts at: 1339 1339
Size: 4880
AflIII A'CryG_T
Cuts at: 1193 2232 3336 4313 1193
Size: 1039 1104 977 1760
Fragments arranged by size:
1760 1104 1039 977
AgeI A'CCGG_T
Cuts at: 3268 3268
Size: 4880
AlwNI CAG_nnn'CTG
Cuts at: 504 819 1677 2185 3752 504
Size: 315 858 508 1567 1632
Fragments arranged by size:
1632 1567 858 508 315
ApaLI G'TGCA_C
Cuts at: 3650 3650
Size: 4880
ApoI r'AATT_y
Cuts at: 429 1884 4233 4489 429
Size: 1455 2349 256 820
Fragments arranged by size:
2349 1455 820 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4880
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
AvaI C'yCGr_G
Cuts at: 115 152 253 1048 1117 1321 1849 1900 115
Size: 37 101 795 69 204 528 51 3095
Fragments arranged by size:
3095 795 528 204 101 69 51 37
BamHI G'GATC_C
Cuts at: 1905 1905
Size: 4880
BanI G'GyrC_C
Cuts at: 296 1147 1896 2310 2558 2593 296
Size: 851 749 414 248 35 2583
Fragments arranged by size:
2583 851 749 414 248 35
BanII G_rGCy'C
Cuts at: 116 1825 1894 2924 116
Size: 1709 69 1030 2072
Fragments arranged by size:
2072 1709 1030 69
BbeI G_GCGC'C
Cuts at: 300 2314 2562 300
Size: 2014 248 2618
Fragments arranged by size:
2618 2014 248
BbsI GAAGACnn'nnnn_
Cuts at: 966 966
Size: 4880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 120 154 1235 1269 120
Size: 34 1081 34 3731
Fragments arranged by size:
3731 1081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2778 3539 4880 2778
Size: 761 1341 2778
Fragments arranged by size:
2778 1341 761
BclI T'GATC_A
Cuts at: 2400 2400
Size: 4880
BglI GCCn_nnn'nGGC
Cuts at: 294 950 294
Size: 656 4224
BglII A'GATC_T
Cuts at: 989 2395 4432 989
Size: 1406 2037 1437
Fragments arranged by size:
2037 1437 1406
BlpI GC'TnA_GC
Cuts at: 247 337 1992 247
Size: 90 1655 3135
Fragments arranged by size:
3135 1655 90
Bme1580I G_kGCm'C
Cuts at: 1118 1150 1425 1631 2505 2598 3654 1118
Size: 32 275 206 874 93 1056 2344
Fragments arranged by size:
2344 1056 874 275 206 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 2092 2173 2508 4652 2092
Size: 81 335 2144 2320
Fragments arranged by size:
2320 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 4880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 526 841 1024 1510 1600 193
Size: 333 315 183 486 90 3473
Fragments arranged by size:
3473 486 333 315 183 90
Bpu10I CC'TnA_GC
Cuts at: 565 565
Size: 4880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 904 2056 2935 3427 3725 130
Size: 774 1152 879 492 298 1285
Fragments arranged by size:
1285 1152 879 774 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 862 1651 4584 862
Size: 789 2933 1158
Fragments arranged by size:
2933 1158 789
BsaAI yAC'GTr
Cuts at: 2233 2233
Size: 4880
BsaBI GATnn'nnATC
Cuts at: 6 2394 4431 6
Size: 2388 2037 455
Fragments arranged by size:
2388 2037 455
BsaHI Gr'CG_yC
Cuts at: 297 2311 2559 3261 4757 297
Size: 2014 248 702 1496 420
Fragments arranged by size:
2014 1496 702 420 248
BsaWI w'CCGG_w
Cuts at: 1167 2069 2590 3268 3542 3689 4100 1167
Size: 902 521 678 274 147 411 1947
Fragments arranged by size:
1947 902 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1648 1678 1648
Size: 30 4850
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1306 1339 1366 1306
Size: 33 27 4820
Fragments arranged by size:
4820 33 27
BseYI C'CCAG_C
Cuts at: 497 1473 1844 2305 3063 3640 4136 497
Size: 976 371 461 758 577 496 1241
Fragments arranged by size:
1241 976 758 577 496 461 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 997 1724 477
Size: 520 727 3633
Fragments arranged by size:
3633 727 520
BsiEI CG_ry'CG
Cuts at: 104 2422 2468 3676 104
Size: 2318 46 1208 1308
Fragments arranged by size:
2318 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 116 1442 1825 1894 2672 2862 3654 116
Size: 1326 383 69 778 190 792 1342
Fragments arranged by size:
1342 1326 792 778 383 190 69
Bsp1286I G_dGCh'C
Cuts at: 116 1118 1150 1425 1442 1631 1825 1894 2505
Size: 1002 32 275 17 189 194 69 611
Cuts at: 2505 2598 2672 2862 2924 3654 116
Size: 93 74 190 62 730 1342
Fragments arranged by size:
1342 1002 730 611 275 194 190 189
93 74 69 62 32 17
BspEI T'CCGG_A
Cuts at: 1167 4100 1167
Size: 2933 1947
BspHI T'CATG_A
Cuts at: 4056 4056
Size: 4880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1019 1746 1930 2144 2446 2827 1019
Size: 727 184 214 302 381 3072
Fragments arranged by size:
3072 727 381 302 214 184
BsrBI CCG'CTC
Cuts at: 50 282 1302 1358 3172 3226 4710 4875 50
Size: 232 1020 56 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 1020 232 165 56 55 54
BsrDI GCAATG_nn'
Cuts at: 2792 2792
Size: 4880
BsrFI r'CCGG_y
Cuts at: 2878 3059 3268 2878
Size: 181 209 4490
Fragments arranged by size:
4490 209 181
BsrGI T'GTAC_A
Cuts at: 1031 1031
Size: 4880
BssSI C'ACGA_G
Cuts at: 324 556 3151 3509 324
Size: 232 2595 358 1695
Fragments arranged by size:
2595 1695 358 232
BstAPI GCAn_nnn'nTGC
Cuts at: 2257 2257
Size: 4880
BstBI TT'CG_AA
Cuts at: 3241 3241
Size: 4880
BstYI r'GATC_y
Cuts at: 1 815 989 1905 2372 2395 2730 2976 3988
Size: 814 174 916 467 23 335 246 1012
Cuts at: 3988 4074 4086 4432 1
Size: 86 12 346 449
Fragments arranged by size:
1012 916 814 467 449 346 335 246
174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 464 464
Size: 4880
BtgI C'CryG_G
Cuts at: 107 794 1071 1151 107
Size: 687 277 80 3836
Fragments arranged by size:
3836 687 277 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2830 3011 3174 2830
Size: 181 163 4536
Fragments arranged by size:
4536 181 163
BtsI GCAGTG_nn'
Cuts at: 628 1425 1629 2123 628
Size: 797 204 494 3385
Fragments arranged by size:
3385 797 494 204
DraI TTT'AAA
Cuts at: 1879 1879
Size: 4880
DrdI GACnn_nn'nnGTC
Cuts at: 1775 2586 3444 1775
Size: 811 858 3211
Fragments arranged by size:
3211 858 811
EaeI y'GGCC_r
Cuts at: 368 1074 2465 2639 3030 3057 4402 368
Size: 706 1391 174 391 27 1345 846
Fragments arranged by size:
1391 1345 846 706 391 174 27
EagI C'GGCC_G
Cuts at: 2465 2465
Size: 4880
EarI CTCTTCn'nnn_
Cuts at: 230 1777 1813 2903 3113 230
Size: 1547 36 1090 210 1997
Fragments arranged by size:
1997 1547 1090 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3088 3398 3544 4690 3088
Size: 310 146 1146 3278
Fragments arranged by size:
3278 1146 310 146
EcoICRI GAG'CTC
Cuts at: 114 1823 1892 114
Size: 1709 69 3102
Fragments arranged by size:
3102 1709 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 526 648 841 1024 1063 1510 1600 1758
Size: 333 122 193 183 39 447 90 158
Cuts at: 1758 2705 3137 3884 4152 193
Size: 947 432 747 268 921
Fragments arranged by size:
947 921 747 447 432 333 268 193
183 158 122 90 39
EcoNI CCTnn'n_nnAGG
Cuts at: 532 1381 532
Size: 849 4031
EcoO109I rG'GnC_Cy
Cuts at: 448 468 514 1008 1038 1317 1386 1854 2019
Size: 20 46 494 30 279 69 468 165
Cuts at: 2019 448
Size: 3309
Fragments arranged by size:
3309 494 468 279 165 69 46 30
20
EcoRI G'AATT_C
Cuts at: 1884 1884
Size: 4880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1479 2899 1479
Size: 1420 3460
FspI TGC'GCA
Cuts at: 2661 2661
Size: 4880
HaeII r_GCGC'y
Cuts at: 300 802 1219 1341 2314 2562 3584 4563 300
Size: 502 417 122 973 248 1022 979 617
Fragments arranged by size:
1022 979 973 617 502 417 248 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2722 2752 2754 2784 2722
Size: 30 2 30 4818
Fragments arranged by size:
4818 30 30 2
HincII GTy'rAC
Cuts at: 1919 1919
Size: 4880
KasI G'GCGC_C
Cuts at: 296 2310 2558 296
Size: 2014 248 2618
Fragments arranged by size:
2618 2014 248
KpnI G_GTAC'C
Cuts at: 1900 1900
Size: 4880
MluI A'CGCG_T
Cuts at: 4313 4313
Size: 4880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3551 3735 3551
Size: 184 4696
MscI TGG'CCA
Cuts at: 2641 2641
Size: 4880
MslI CAynn'nnrTG
Cuts at: 2869 2996 2869
Size: 127 4753
MspA1I CmG'CkG
Cuts at: 344 480 501 1991 2305 2665 3678 3923 344
Size: 136 21 1490 314 360 1013 245 1301
Fragments arranged by size:
1490 1301 1013 360 314 245 136 21
NaeI GCC'GGC
Cuts at: 3061 3061
Size: 4880
NarI GG'CG_CC
Cuts at: 297 2311 2559 297
Size: 2014 248 2618
Fragments arranged by size:
2618 2014 248
NcoI C'CATG_G
Cuts at: 107 794 107
Size: 687 4193
NgoMIV G'CCGG_C
Cuts at: 3059 3059
Size: 4880
NspI r_CATG'y
Cuts at: 410 1114 1488 1933 2433 3340 410
Size: 704 374 445 500 907 1950
Fragments arranged by size:
1950 907 704 500 445 374
PciI A'CATG_T
Cuts at: 3336 3336
Size: 4880
PflMI CCAn_nnn'nTGG
Cuts at: 504 1637 1868 504
Size: 1133 231 3516
Fragments arranged by size:
3516 1133 231
PfoI T'CCnGG_A
Cuts at: 356 533 818 1670 356
Size: 177 285 852 3566
Fragments arranged by size:
3566 852 285 177
PmeI GTTT'AAAC
Cuts at: 1879 1879
Size: 4880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 4019
Size: 32 4848
PpuMI rG'GwC_Cy
Cuts at: 448 468 514 1008 1038 1317 1386 448
Size: 20 46 494 30 279 69 3942
Fragments arranged by size:
3942 494 279 69 46 30 20
PspXI vC'TCGA_Gb
Cuts at: 253 253
Size: 4880
PstI C_TGCA'G
Cuts at: 623 1766 1806 1927 2612 623
Size: 1143 40 121 685 2891
Fragments arranged by size:
2891 1143 685 121 40
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4880
PvuII CAG'CTG
Cuts at: 480 2305 2665 480
Size: 1825 360 2695
Fragments arranged by size:
2695 1825 360
RsrII CG'GwC_CG
Cuts at: 3075 3075
Size: 4880
SacI G_AGCT'C
Cuts at: 116 1825 1894 116
Size: 1709 69 3102
Fragments arranged by size:
3102 1709 69
SalI G'TCGA_C
Cuts at: 1917 1917
Size: 4880
SanDI GG'GwC_CC
Cuts at: 514 514
Size: 4880
SapI GCTCTTCn'nnn_
Cuts at: 230 1813 2903 3113 230
Size: 1583 1090 210 1997
Fragments arranged by size:
1997 1583 1090 210
SbfI CC_TGCA'GG
Cuts at: 1927 1927
Size: 4880
ScaI AGT'ACT
Cuts at: 4397 4397
Size: 4880
SfcI C'TryA_G
Cuts at: 33 619 1762 1802 1923 2608 3601 3792 33
Size: 586 1143 40 121 685 993 191 1121
Fragments arranged by size:
1143 1121 993 685 586 191 121 40
SfoI GGC'GCC
Cuts at: 298 2312 2560 298
Size: 2014 248 2618
Fragments arranged by size:
2618 2014 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4880
SmaI CCC'GGG
Cuts at: 154 1902 154
Size: 1748 3132
SmlI C'TyrA_G
Cuts at: 115 145 253 919 2035 2950 3442 3704 115
Size: 30 108 666 1116 915 492 262 1291
Fragments arranged by size:
1291 1116 915 666 492 262 108 30
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4880
SphI G_CATG'C
Cuts at: 1488 1933 2433 1488
Size: 445 500 3935
Fragments arranged by size:
3935 500 445
StyI C'CwwG_G
Cuts at: 107 794 872 2014 2368 107
Size: 687 78 1142 354 2619
Fragments arranged by size:
2619 1142 687 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 511 1675 2463 2596 3055 3264 511
Size: 1164 788 133 459 209 2127
Fragments arranged by size:
2127 1164 788 459 209 133
TatI w'GTAC_w
Cuts at: 1031 4395 1031
Size: 3364 1516
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 393 425 868 900 1091 1123 1648 1680 4397
Size: 32 443 32 191 32 525 32 2717
Cuts at: 4397 4429 393
Size: 32 844
Fragments arranged by size:
2717 844 525 443 191 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2677 2677
Size: 4880
XbaI T'CTAG_A
Cuts at: 1911 1911
Size: 4880
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1718 2375 1718
Size: 657 4223
XhoI C'TCGA_G
Cuts at: 115 253 115
Size: 138 4742
XmaI C'CCGG_G
Cuts at: 152 1900 152
Size: 1748 3132
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI