Restriction map for
pF1KB9910
(Circular) (Six-base) MAPSORT of: pF1KB9910.seq Check: 3457 from: 1 to: 4883
pF1KB9910 4883 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1495 1622 1495
Size: 127 4756
AatII G_ACGT'C
Cuts at: 1727 1727
Size: 4883
AccI GT'mk_AC
Cuts at: 1921 1921
Size: 4883
Acc65I G'GTAC_C
Cuts at: 656 1798 1899 656
Size: 1142 101 3640
Fragments arranged by size:
3640 1142 101
AclI AA'CG_TT
Cuts at: 1877 4723 1877
Size: 2846 2037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 539 617 1276 1411 2708 3140 3887 4155
Size: 214 78 659 135 1297 432 747 268
Cuts at: 4155 325
Size: 1053
Fragments arranged by size:
1297 1053 747 659 432 268 214 135
78
AfeI AGC'GCT
Cuts at: 907 907
Size: 4883
AflIII A'CryG_T
Cuts at: 815 2235 3339 4316 815
Size: 1420 1104 977 1382
Fragments arranged by size:
1420 1382 1104 977
AgeI A'CCGG_T
Cuts at: 3271 3271
Size: 4883
AleI CACnn'nnGTG
Cuts at: 373 676 1714 373
Size: 303 1038 3542
Fragments arranged by size:
3542 1038 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 926 926
Size: 4883
AlwNI CAG_nnn'CTG
Cuts at: 983 1487 1500 1515 2188 3755 983
Size: 504 13 15 673 1567 2111
Fragments arranged by size:
2111 1567 673 504 15 13
ApaLI G'TGCA_C
Cuts at: 3653 3653
Size: 4883
ApoI r'AATT_y
Cuts at: 256 1887 4236 4492 256
Size: 1631 2349 256 647
Fragments arranged by size:
2349 1631 647 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
AvaI C'yCGr_G
Cuts at: 266 711 994 1903 266
Size: 445 283 909 3246
Fragments arranged by size:
3246 909 445 283
BamHI G'GATC_C
Cuts at: 1908 1908
Size: 4883
BanI G'GyrC_C
Cuts at: 127 656 1798 1899 2313 2561 2596 127
Size: 529 1142 101 414 248 35 2414
Fragments arranged by size:
2414 1142 529 414 248 101 35
BanII G_rGCy'C
Cuts at: 874 1020 1146 1897 2927 874
Size: 146 126 751 1030 2830
Fragments arranged by size:
2830 1030 751 146 126
BbeI G_GCGC'C
Cuts at: 131 2317 2565 131
Size: 2186 248 2449
Fragments arranged by size:
2449 2186 248
BbsI GAAGACnn'nnnn_
Cuts at: 1594 1594
Size: 4883
BciVI GTATCCnnnnn_n'
Cuts at: 2781 3542 4883 2781
Size: 761 1341 2781
Fragments arranged by size:
2781 1341 761
BclI T'GATC_A
Cuts at: 476 2403 476
Size: 1927 2956
BglI GCCn_nnn'nGGC
Cuts at: 988 988
Size: 4883
BglII A'GATC_T
Cuts at: 2398 4435 2398
Size: 2037 2846
BlpI GC'TnA_GC
Cuts at: 1995 1995
Size: 4883
Bme1580I G_kGCm'C
Cuts at: 961 2508 2601 3657 961
Size: 1547 93 1056 2187
Fragments arranged by size:
2187 1547 1056 93
BmgBI CAC'GTC
Cuts at: 1563 1563
Size: 4883
BmrI ACTGGGnnnn_n'
Cuts at: 2095 2176 2511 4655 2095
Size: 81 335 2144 2323
Fragments arranged by size:
2323 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3250 3250
Size: 4883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 317 1687 1798 230
Size: 87 1370 111 3315
Fragments arranged by size:
3315 1370 111 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2059 2938 3430 3728 1597
Size: 462 879 492 298 2752
Fragments arranged by size:
2752 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 335 523 1204 4587 335
Size: 188 681 3383 631
Fragments arranged by size:
3383 681 631 188
BsaAI yAC'GTr
Cuts at: 569 818 2236 569
Size: 249 1418 3216
Fragments arranged by size:
3216 1418 249
BsaBI GATnn'nnATC
Cuts at: 6 2397 4434 6
Size: 2391 2037 455
Fragments arranged by size:
2391 2037 455
BsaHI Gr'CG_yC
Cuts at: 128 932 1724 2314 2562 3264 4760 128
Size: 804 792 590 248 702 1496 251
Fragments arranged by size:
1496 804 792 702 590 251 248
BsaWI w'CCGG_w
Cuts at: 722 1077 2072 2593 3271 3545 3692 4103 722
Size: 355 995 521 678 274 147 411 1502
Fragments arranged by size:
1502 995 678 521 411 355 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 1751 1781 802
Size: 30 919 30 3904
Fragments arranged by size:
3904 919 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 767 874 1064 1724 767
Size: 107 190 660 3926
Fragments arranged by size:
3926 660 190 107
BseYI C'CCAG_C
Cuts at: 534 1553 2308 3066 3643 4139 534
Size: 1019 755 758 577 496 1278
Fragments arranged by size:
1278 1019 758 755 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 922 1486 454
Size: 468 564 3851
Fragments arranged by size:
3851 564 468
BsiEI CG_ry'CG
Cuts at: 104 164 2425 2471 3679 104
Size: 60 2261 46 1208 1308
Fragments arranged by size:
2261 1308 1208 60 46
BsiHKAI G_wGCw'C
Cuts at: 722 815 1345 1550 1897 2675 2865 3657 722
Size: 93 530 205 347 778 190 792 1948
Fragments arranged by size:
1948 792 778 530 347 205 190 93
BsiWI C'GTAC_G
Cuts at: 1190 1190
Size: 4883
BsmI GAATG_Cn'
Cuts at: 250 250
Size: 4883
Bsp1286I G_dGCh'C
Cuts at: 722 815 874 961 1020 1146 1345 1550 1897
Size: 93 59 87 59 126 199 205 347
Cuts at: 1897 2508 2601 2675 2865 2927 3657 722
Size: 611 93 74 190 62 730 1948
Fragments arranged by size:
1948 730 611 347 205 199 190 126
93 93 87 74 62 59 59
BspEI T'CCGG_A
Cuts at: 722 4103 722
Size: 3381 1502
BspHI T'CATG_A
Cuts at: 927 4059 927
Size: 3132 1751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 401 426 1495 1622 1786 1933 2147 2449 2830
Size: 25 1069 127 164 147 214 302 381
Cuts at: 2830 401
Size: 2454
Fragments arranged by size:
2454 1069 381 302 214 164 147 127
25
BsrBI CCG'CTC
Cuts at: 50 3175 3229 4713 4878 50
Size: 3125 54 1484 165 55
Fragments arranged by size:
3125 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1385 1727 2795 1385
Size: 342 1068 3473
Fragments arranged by size:
3473 1068 342
BsrFI r'CCGG_y
Cuts at: 2881 3062 3271 2881
Size: 181 209 4493
Fragments arranged by size:
4493 209 181
BssSI C'ACGA_G
Cuts at: 3154 3512 3154
Size: 358 4525
BstAPI GCAn_nnn'nTGC
Cuts at: 2260 2260
Size: 4883
BstBI TT'CG_AA
Cuts at: 3244 3244
Size: 4883
BstYI r'GATC_y
Cuts at: 1 1908 2375 2398 2733 2979 3991 4077 4089
Size: 1907 467 23 335 246 1012 86 12
Cuts at: 4089 4435 1
Size: 346 449
Fragments arranged by size:
1907 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 804 107
Size: 697 4186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1776 2833 3014 3177 1776
Size: 1057 181 163 3482
Fragments arranged by size:
3482 1057 181 163
BtsI GCAGTG_nn'
Cuts at: 1591 1848 2126 1591
Size: 257 278 4348
Fragments arranged by size:
4348 278 257
DraI TTT'AAA
Cuts at: 1134 1882 1134
Size: 748 4135
DrdI GACnn_nn'nnGTC
Cuts at: 1039 2589 3447 1039
Size: 1550 858 2475
Fragments arranged by size:
2475 1550 858
EaeI y'GGCC_r
Cuts at: 161 189 398 2468 2642 3033 3060 4405 161
Size: 28 209 2070 174 391 27 1345 639
Fragments arranged by size:
2070 1345 639 391 209 174 28 27
EagI C'GGCC_G
Cuts at: 161 2468 161
Size: 2307 2576
EarI CTCTTCn'nnn_
Cuts at: 301 460 2906 3116 301
Size: 159 2446 210 2068
Fragments arranged by size:
2446 2068 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3091 3401 3547 4693 3091
Size: 310 146 1146 3281
Fragments arranged by size:
3281 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1895 1895
Size: 4883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 317 325 539 617 1276 1411 1687 1798
Size: 87 8 214 78 659 135 276 111
Cuts at: 1798 2708 3140 3887 4155 230
Size: 910 432 747 268 958
Fragments arranged by size:
958 910 747 659 432 276 268 214
135 111 87 78 8
EcoNI CCTnn'n_nnAGG
Cuts at: 975 975
Size: 4883
EcoO109I rG'GnC_Cy
Cuts at: 576 980 1316 1512 2022 576
Size: 404 336 196 510 3437
Fragments arranged by size:
3437 510 404 336 196
EcoRI G'AATT_C
Cuts at: 1887 1887
Size: 4883
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 781 2902 781
Size: 2121 2762
FspI TGC'GCA
Cuts at: 883 2664 883
Size: 1781 3102
HaeII r_GCGC'y
Cuts at: 131 909 2317 2565 3587 4566 131
Size: 778 1408 248 1022 979 448
Fragments arranged by size:
1408 1022 979 778 448 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1223 1243 1255 1275 2725 2755 2757 2787 1223
Size: 20 12 20 1450 30 2 30 3319
Fragments arranged by size:
3319 1450 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 1262 1922 1262
Size: 660 4223
HindIII A'AGCT_T
Cuts at: 637 1531 637
Size: 894 3989
KasI G'GCGC_C
Cuts at: 127 2313 2561 127
Size: 2186 248 2449
Fragments arranged by size:
2449 2186 248
KpnI G_GTAC'C
Cuts at: 660 1802 1903 660
Size: 1142 101 3640
Fragments arranged by size:
3640 1142 101
MluI A'CGCG_T
Cuts at: 4316 4316
Size: 4883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3554 3738 3554
Size: 184 4699
MscI TGG'CCA
Cuts at: 2644 2644
Size: 4883
MslI CAynn'nnrTG
Cuts at: 373 676 1063 1714 1870 2872 2999 373
Size: 303 387 651 156 1002 127 2257
Fragments arranged by size:
2257 1002 651 387 303 156 127
MspA1I CmG'CkG
Cuts at: 373 877 964 1434 1521 1557 1994 2308 2668
Size: 504 87 470 87 36 437 314 360
Cuts at: 2668 3681 3926 373
Size: 1013 245 1330
Fragments arranged by size:
1330 1013 504 470 437 360 314 245
87 87 36
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 4883
NarI GG'CG_CC
Cuts at: 128 2314 2562 128
Size: 2186 248 2449
Fragments arranged by size:
2449 2186 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4883
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 4883
NspI r_CATG'y
Cuts at: 692 1936 2436 3343 692
Size: 1244 500 907 2232
Fragments arranged by size:
2232 1244 907 500
PasI CC'CwG_GG
Cuts at: 977 977
Size: 4883
PciI A'CATG_T
Cuts at: 3339 3339
Size: 4883
PflMI CCAn_nnn'nTGG
Cuts at: 1185 1185
Size: 4883
PfoI T'CCnGG_A
Cuts at: 1106 1775 1106
Size: 669 4214
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4883
PmlI CAC'GTG
Cuts at: 569 569
Size: 4883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 4851
PpuMI rG'GwC_Cy
Cuts at: 576 1512 576
Size: 936 3947
PshAI GACnn'nnGTC
Cuts at: 935 935
Size: 4883
PsiI TTA'TAA
Cuts at: 587 587
Size: 4883
PstI C_TGCA'G
Cuts at: 1439 1520 1930 2615 1439
Size: 81 410 685 3707
Fragments arranged by size:
3707 685 410 81
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4883
PvuII CAG'CTG
Cuts at: 964 1434 1521 1557 2308 2668 964
Size: 470 87 36 751 360 3179
Fragments arranged by size:
3179 751 470 360 87 36
RsrII CG'GwC_CG
Cuts at: 3078 3078
Size: 4883
SacI G_AGCT'C
Cuts at: 1897 1897
Size: 4883
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4883
SapI GCTCTTCn'nnn_
Cuts at: 2906 3116 2906
Size: 210 4673
SbfI CC_TGCA'GG
Cuts at: 1930 1930
Size: 4883
ScaI AGT'ACT
Cuts at: 4400 4400
Size: 4883
SfcI C'TryA_G
Cuts at: 33 1435 1516 1926 2611 3604 3795 33
Size: 1402 81 410 685 993 191 1121
Fragments arranged by size:
1402 1121 993 685 410 191 81
SfoI GGC'GCC
Cuts at: 129 2315 2563 129
Size: 2186 248 2449
Fragments arranged by size:
2449 2186 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
SmaI CCC'GGG
Cuts at: 268 1905 268
Size: 1637 3246
SmlI C'TyrA_G
Cuts at: 711 1576 2038 2953 3445 3707 711
Size: 865 462 915 492 262 1887
Fragments arranged by size:
1887 915 865 492 462 262
SpeI A'CTAG_T
Cuts at: 67 1326 67
Size: 1259 3624
SphI G_CATG'C
Cuts at: 692 1936 2436 692
Size: 1244 500 3139
Fragments arranged by size:
3139 1244 500
StyI C'CwwG_G
Cuts at: 107 953 1420 2017 2371 107
Size: 846 467 597 354 2619
Fragments arranged by size:
2619 846 597 467 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1372 1386 2466 2599 3058 3267 1372
Size: 14 1080 133 459 209 2988
Fragments arranged by size:
2988 1080 459 209 133 14
TatI w'GTAC_w
Cuts at: 1632 4398 1632
Size: 2766 2117
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4400 4432 4400
Size: 32 4851
Tth111I GACn'n_nGTC
Cuts at: 2680 2680
Size: 4883
XbaI T'CTAG_A
Cuts at: 1914 1914
Size: 4883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2378 2378
Size: 4883
XhoI C'TCGA_G
Cuts at: 711 711
Size: 4883
XmaI C'CCGG_G
Cuts at: 266 1903 266
Size: 1637 3246
XmnI GAAnn'nnTTC
Cuts at: 1283 1283
Size: 4883
ZraI GAC'GTC
Cuts at: 1725 1725
Size: 4883
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI ApaI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI Bpu10I BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI