Restriction map for
pF1KB9914
(Circular) (Six-base) MAPSORT of: pF1KB9914.seq Check: 5219 from: 1 to: 4712
pF1KB9914 4712 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 716 1642 716
Size: 926 3786
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 1728 1728
Size: 4712
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 473 774 1306 1606 2537 2969 3716 3984
Size: 305 301 532 300 931 432 747 268
Cuts at: 3984 168
Size: 896
Fragments arranged by size:
931 896 747 532 432 305 301 300
268
AflIII A'CryG_T
Cuts at: 486 758 1010 2064 3168 4145 486
Size: 272 252 1054 1104 977 1053
Fragments arranged by size:
1104 1054 1053 977 272 252
AgeI A'CCGG_T
Cuts at: 3100 3100
Size: 4712
AlwNI CAG_nnn'CTG
Cuts at: 2017 3584 2017
Size: 1567 3145
ApaLI G'TGCA_C
Cuts at: 3482 3482
Size: 4712
ApoI r'AATT_y
Cuts at: 315 1248 1716 4065 4321 315
Size: 933 468 2349 256 706
Fragments arranged by size:
2349 933 706 468 256
AseI AT'TA_AT
Cuts at: 20 382 1183 20
Size: 362 801 3549
Fragments arranged by size:
3549 801 362
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 772 1565 1732 772
Size: 793 167 3752
Fragments arranged by size:
3752 793 167
BamHI G'GATC_C
Cuts at: 112 1737 112
Size: 1625 3087
BanI G'GyrC_C
Cuts at: 1422 1728 2142 2390 2425 1422
Size: 306 414 248 35 3709
Fragments arranged by size:
3709 414 306 248 35
BanII G_rGCy'C
Cuts at: 731 1726 2756 731
Size: 995 1030 2687
Fragments arranged by size:
2687 1030 995
BbeI G_GCGC'C
Cuts at: 2146 2394 2146
Size: 248 4464
BbsI GAAGACnn'nnnn_
Cuts at: 1630 1630
Size: 4712
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3371 4712 2610
Size: 761 1341 2610
Fragments arranged by size:
2610 1341 761
BclI T'GATC_A
Cuts at: 1175 2232 1175
Size: 1057 3655
BglII A'GATC_T
Cuts at: 2227 4264 2227
Size: 2037 2675
BlpI GC'TnA_GC
Cuts at: 742 1824 742
Size: 1082 3630
Bme1580I G_kGCm'C
Cuts at: 2337 2430 3486 2337
Size: 93 1056 3563
Fragments arranged by size:
3563 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1924 2005 2340 4484 1924
Size: 81 335 2144 2152
Fragments arranged by size:
2152 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 435 954 3079 435
Size: 519 2125 2068
Fragments arranged by size:
2125 2068 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 186 659 772 910 1090 108
Size: 78 473 113 138 180 3730
Fragments arranged by size:
3730 473 180 138 113 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 1888 2767 3259 3557 405
Size: 1483 879 492 298 1560
Fragments arranged by size:
1560 1483 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 294 336 673 4416 294
Size: 42 337 3743 590
Fragments arranged by size:
3743 590 337 42
BsaAI yAC'GTr
Cuts at: 1013 2065 1013
Size: 1052 3660
BsaBI GATnn'nnATC
Cuts at: 6 2226 4263 6
Size: 2220 2037 455
Fragments arranged by size:
2220 2037 455
BsaHI Gr'CG_yC
Cuts at: 281 713 1601 1639 2143 2391 3093 4589 281
Size: 432 888 38 504 248 702 1496 404
Fragments arranged by size:
1496 888 702 504 432 404 248 38
BsaWI w'CCGG_w
Cuts at: 1901 2422 3100 3374 3521 3932 1901
Size: 521 678 274 147 411 2681
Fragments arranged by size:
2681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 184 214 745 775 1114 1144 184
Size: 30 531 30 339 30 3752
Fragments arranged by size:
3752 531 339 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 111 571 623 1282 1549 1594 111
Size: 460 52 659 267 45 3229
Fragments arranged by size:
3229 659 460 267 52 45
BseYI C'CCAG_C
Cuts at: 2137 2895 3472 3968 2137
Size: 758 577 496 2881
Fragments arranged by size:
2881 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1395 2254 2300 3508 104
Size: 1291 859 46 1208 1308
Fragments arranged by size:
1308 1291 1208 859 46
BsiHKAI G_wGCw'C
Cuts at: 758 1274 1726 2504 2694 3486 758
Size: 516 452 778 190 792 1984
Fragments arranged by size:
1984 792 778 516 452 190
BsmI GAATG_Cn'
Cuts at: 167 427 167
Size: 260 4452
Bsp1286I G_dGCh'C
Cuts at: 731 758 1274 1726 2337 2430 2504 2694 2756
Size: 27 516 452 611 93 74 190 62
Cuts at: 2756 3486 731
Size: 730 1957
Fragments arranged by size:
1957 730 611 516 452 190 93 74
62 27
BspEI T'CCGG_A
Cuts at: 3932 3932
Size: 4712
BspHI T'CATG_A
Cuts at: 3888 3888
Size: 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 393 674 1762 1976 2278 2659 393
Size: 281 1088 214 302 381 2446
Fragments arranged by size:
2446 1088 381 302 281 214
BsrBI CCG'CTC
Cuts at: 50 3004 3058 4542 4707 50
Size: 2954 54 1484 165 55
Fragments arranged by size:
2954 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2624 2624
Size: 4712
BsrFI r'CCGG_y
Cuts at: 131 235 2710 2891 3100 131
Size: 104 2475 181 209 1743
Fragments arranged by size:
2475 1743 209 181 104
BsrGI T'GTAC_A
Cuts at: 899 899
Size: 4712
BssSI C'ACGA_G
Cuts at: 2983 3341 2983
Size: 358 4354
BstAPI GCAn_nnn'nTGC
Cuts at: 194 1210 2089 194
Size: 1016 879 2817
Fragments arranged by size:
2817 1016 879
BstBI TT'CG_AA
Cuts at: 3073 3073
Size: 4712
BstXI CCAn_nnnn'nTGG
Cuts at: 150 1200 150
Size: 1050 3662
BstYI r'GATC_y
Cuts at: 1 112 1100 1160 1737 2204 2227 2562 2808
Size: 111 988 60 577 467 23 335 246
Cuts at: 2808 3820 3906 3918 4264 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 988 577 467 449 346 335 246
111 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 811 811
Size: 4712
BtgI C'CryG_G
Cuts at: 107 284 1088 1526 107
Size: 177 804 438 3293
Fragments arranged by size:
3293 804 438 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 213 656 2662 2843 3006 213
Size: 443 2006 181 163 1919
Fragments arranged by size:
2006 1919 443 181 163
BtsI GCAGTG_nn'
Cuts at: 873 1080 1955 873
Size: 207 875 3630
Fragments arranged by size:
3630 875 207
ClaI AT'CG_AT
Cuts at: 158 158
Size: 4712
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DrdI GACnn_nn'nnGTC
Cuts at: 286 2418 3276 286
Size: 2132 858 1722
Fragments arranged by size:
2132 1722 858
EaeI y'GGCC_r
Cuts at: 350 566 971 1190 2297 2471 2862 2889 4234
Size: 216 405 219 1107 174 391 27 1345
Cuts at: 4234 350
Size: 828
Fragments arranged by size:
1345 1107 828 405 391 219 216 174
27
EagI C'GGCC_G
Cuts at: 2297 2297
Size: 4712
EarI CTCTTCn'nnn_
Cuts at: 1525 2735 2945 1525
Size: 1210 210 3292
Fragments arranged by size:
3292 1210 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2920 3230 3376 4522 2920
Size: 310 146 1146 3110
Fragments arranged by size:
3110 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1724 1724
Size: 4712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 168 186 473 659 772 774 910 1090
Size: 60 18 287 186 113 2 136 180
Cuts at: 1090 1306 1606 2537 2969 3716 3984 108
Size: 216 300 931 432 747 268 836
Fragments arranged by size:
931 836 747 432 300 287 268 216
186 180 136 113 60 18 2
EcoO109I rG'GnC_Cy
Cuts at: 690 1851 690
Size: 1161 3551
EcoRI G'AATT_C
Cuts at: 315 1716 315
Size: 1401 3311
EcoRV GAT'ATC
Cuts at: 951 1314 951
Size: 363 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 198 723 2731 198
Size: 525 2008 2179
Fragments arranged by size:
2179 2008 525
FspI TGC'GCA
Cuts at: 2493 2493
Size: 4712
HaeII r_GCGC'y
Cuts at: 2146 2394 3416 4395 2146
Size: 248 1022 979 2463
Fragments arranged by size:
2463 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2554 2584 2586 2616 2554
Size: 30 2 30 4650
Fragments arranged by size:
4650 30 30 2
HincII GTy'rAC
Cuts at: 629 1751 629
Size: 1122 3590
KasI G'GCGC_C
Cuts at: 2142 2390 2142
Size: 248 4464
KpnI G_GTAC'C
Cuts at: 1732 1732
Size: 4712
MluI A'CGCG_T
Cuts at: 486 4145 486
Size: 3659 1053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1157 1188 3383 3567 1157
Size: 31 2195 184 2302
Fragments arranged by size:
2302 2195 184 31
MscI TGG'CCA
Cuts at: 352 1192 2473 352
Size: 840 1281 2591
Fragments arranged by size:
2591 1281 840
MslI CAynn'nnrTG
Cuts at: 1198 2701 2828 1198
Size: 1503 127 3082
Fragments arranged by size:
3082 1503 127
MspA1I CmG'CkG
Cuts at: 970 1068 1823 2137 2497 3510 3755 970
Size: 98 755 314 360 1013 245 1927
Fragments arranged by size:
1927 1013 755 360 314 245 98
NaeI GCC'GGC
Cuts at: 2893 2893
Size: 4712
NarI GG'CG_CC
Cuts at: 2143 2391 2143
Size: 248 4464
NcoI C'CATG_G
Cuts at: 107 284 1526 107
Size: 177 1242 3293
Fragments arranged by size:
3293 1242 177
NgoMIV G'CCGG_C
Cuts at: 2891 2891
Size: 4712
NspI r_CATG'y
Cuts at: 762 1213 1765 2265 3172 762
Size: 451 552 500 907 2302
Fragments arranged by size:
2302 907 552 500 451
PciI A'CATG_T
Cuts at: 758 3168 758
Size: 2410 2302
PflMI CCAn_nnn'nTGG
Cuts at: 987 1147 987
Size: 160 4552
PfoI T'CCnGG_A
Cuts at: 926 926
Size: 4712
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4680
PstI C_TGCA'G
Cuts at: 922 1550 1759 2444 922
Size: 628 209 685 3190
Fragments arranged by size:
3190 685 628 209
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 2137 2497 2137
Size: 360 4352
RsrII CG'GwC_CG
Cuts at: 1044 2907 1044
Size: 1863 2849
SacI G_AGCT'C
Cuts at: 1726 1726
Size: 4712
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 1525 2735 2945 1525
Size: 1210 210 3292
Fragments arranged by size:
3292 1210 210
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 193 4229 193
Size: 4036 676
SfcI C'TryA_G
Cuts at: 33 843 918 1257 1546 1755 2440 3433 3624
Size: 810 75 339 289 209 685 993 191
Cuts at: 3624 33
Size: 1121
Fragments arranged by size:
1121 993 810 685 339 289 209 191
75
SfoI GGC'GCC
Cuts at: 2144 2392 2144
Size: 248 4464
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 1734 1734
Size: 4712
SmlI C'TyrA_G
Cuts at: 420 1867 2782 3274 3536 420
Size: 1447 915 492 262 1596
Fragments arranged by size:
1596 1447 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
SspI AAT'ATT
Cuts at: 1378 1378
Size: 4712
StuI AGG'CCT
Cuts at: 1412 1412
Size: 4712
StyI C'CwwG_G
Cuts at: 107 137 284 1351 1451 1526 1846 2200 107
Size: 30 147 1067 100 75 320 354 2619
Fragments arranged by size:
2619 1067 354 320 147 100 75 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 1451 2295 2428 2887 3096 1344
Size: 107 844 133 459 209 2960
Fragments arranged by size:
2960 844 459 209 133 107
TatI w'GTAC_w
Cuts at: 191 599 899 4227 191
Size: 408 300 3328 676
Fragments arranged by size:
3328 676 408 300
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 494 526 4229 4261 494
Size: 32 3703 32 945
Fragments arranged by size:
3703 945 32 32
Tth111I GACn'n_nGTC
Cuts at: 2509 2509
Size: 4712
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 210 2207 210
Size: 1997 2715
XmaI C'CCGG_G
Cuts at: 1732 1732
Size: 4712
XmnI GAAnn'nnTTC
Cuts at: 1412 1412
Size: 4712
ZraI GAC'GTC
Cuts at: 714 1640 714
Size: 926 3786
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmBI BssHII BstEII BstZ17I
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI