Restriction map for
pF1KB9923
(Circular) (Six-base) MAPSORT of: pF1KB9923.seq Check: 9711 from: 1 to: 4139
pF1KB9923 4139 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1177 1177
Size: 4139
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1000 1105 1964 2396 3143 3411 1000
Size: 105 859 432 747 268 1728
Fragments arranged by size:
1728 859 747 432 268 105
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AleI CACnn'nnGTG
Cuts at: 796 796
Size: 4139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 455 455
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 945 1444 3011 945
Size: 499 1567 2073
Fragments arranged by size:
2073 1567 499
ApaI G_GGCC'C
Cuts at: 354 899 354
Size: 545 3594
ApaLI G'TGCA_C
Cuts at: 430 1003 2909 430
Size: 573 1906 1660
Fragments arranged by size:
1906 1660 573
ApoI r'AATT_y
Cuts at: 1143 3492 3748 1143
Size: 2349 256 1534
Fragments arranged by size:
2349 1534 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 325 347 607 716 1159 325
Size: 22 260 109 443 3305
Fragments arranged by size:
3305 443 260 109 22
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 621 1155 1569 1817 1852 621
Size: 534 414 248 35 2908
Fragments arranged by size:
2908 534 414 248 35
BanII G_rGCy'C
Cuts at: 354 899 932 1153 2183 354
Size: 545 33 221 1030 2310
Fragments arranged by size:
2310 1030 545 221 33
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 1659 1659
Size: 4139
BglI GCCn_nnn'nGGC
Cuts at: 658 864 658
Size: 206 3933
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 737 803 1251 737
Size: 66 448 3625
Fragments arranged by size:
3625 448 66
Bme1580I G_kGCm'C
Cuts at: 354 434 899 1007 1764 1857 2913 354
Size: 80 465 108 757 93 1056 1580
Fragments arranged by size:
1580 1056 757 465 108 93 80
BmgBI CAC'GTC
Cuts at: 1008 1008
Size: 4139
BmrI ACTGGGnnnn_n'
Cuts at: 1022 1351 1432 1767 3911 1022
Size: 329 81 335 2144 1250
Fragments arranged by size:
2144 1250 335 329 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 742 2506 742
Size: 1764 2375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 967
Size: 4139
Bpu10I CC'TnA_GC
Cuts at: 913 913
Size: 4139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1315 2194 2686 2984 448
Size: 867 879 492 298 1603
Fragments arranged by size:
1603 879 867 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 281 3843 281
Size: 3562 577
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1328 1849 2527 2801 2948 3359 1328
Size: 521 678 274 147 411 2108
Fragments arranged by size:
2108 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 811 841 745
Size: 30 36 30 4043
Fragments arranged by size:
4043 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 343 403 661 709 886 1111 343
Size: 60 258 48 177 225 3371
Fragments arranged by size:
3371 258 225 177 60 48
BseYI C'CCAG_C
Cuts at: 458 784 1564 2322 2899 3395 458
Size: 326 780 758 577 496 1202
Fragments arranged by size:
1202 780 758 577 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 1096 962
Size: 134 4005
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 434 691 932 1007 1153 1931 2121 2913 434
Size: 257 241 75 146 778 190 792 1660
Fragments arranged by size:
1660 792 778 257 241 190 146 75
BsmBI CGTCTCn'nnnn_
Cuts at: 989 989
Size: 4139
Bsp1286I G_dGCh'C
Cuts at: 354 434 691 899 932 1007 1153 1764 1857
Size: 80 257 208 33 75 146 611 93
Cuts at: 1857 1931 2121 2183 2913 354
Size: 74 190 62 730 1580
Fragments arranged by size:
1580 730 611 257 208 190 146 93
80 75 74 62 33
BspEI T'CCGG_A
Cuts at: 3359 3359
Size: 4139
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 422 447 1189 1403 1705 2086 422
Size: 25 742 214 302 381 2475
Fragments arranged by size:
2475 742 381 302 214 25
BsrBI CCG'CTC
Cuts at: 50 393 2431 2485 3969 4134 50
Size: 343 2038 54 1484 165 55
Fragments arranged by size:
2038 1484 343 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2051 2051
Size: 4139
BsrFI r'CCGG_y
Cuts at: 365 2137 2318 2527 365
Size: 1772 181 209 1977
Fragments arranged by size:
1977 1772 209 181
BssSI C'ACGA_G
Cuts at: 2410 2768 2410
Size: 358 3781
BstAPI GCAn_nnn'nTGC
Cuts at: 1011 1516 1011
Size: 505 3634
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstEII G'GTnAC_C
Cuts at: 489 489
Size: 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 361 798 361
Size: 437 3702
BstYI r'GATC_y
Cuts at: 1 666 1100 1164 1631 1654 1989 2235 3247
Size: 665 434 64 467 23 335 246 1012
Cuts at: 3247 3333 3345 3691 1
Size: 86 12 346 449
Fragments arranged by size:
1012 665 467 449 434 346 335 246
86 64 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 1382 1382
Size: 4139
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 363 1724 1898 2289 2316 3661 363
Size: 1361 174 391 27 1345 841
Fragments arranged by size:
1361 1345 841 391 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 319 754 872 2162 2372 319
Size: 435 118 1290 210 2086
Fragments arranged by size:
2086 1290 435 210 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 307 2347 2657 2803 3949 307
Size: 2040 310 146 1146 497
Fragments arranged by size:
2040 1146 497 310 146
EcoICRI GAG'CTC
Cuts at: 930 1151 930
Size: 221 3918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1000 1105 1964 2396 3143 3411 967
Size: 33 105 859 432 747 268 1695
Fragments arranged by size:
1695 859 747 432 268 105 33
EcoNI CCTnn'n_nnAGG
Cuts at: 716 730 916 716
Size: 14 186 3939
Fragments arranged by size:
3939 186 14
EcoO109I rG'GnC_Cy
Cuts at: 222 411 516 603 660 721 895 942 1278
Size: 189 105 87 57 61 174 47 336
Cuts at: 1278 222
Size: 3083
Fragments arranged by size:
3083 336 189 174 105 87 61 57
47
EcoRI G'AATT_C
Cuts at: 1143 1143
Size: 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 147 489 2158 147
Size: 342 1669 2128
Fragments arranged by size:
2128 1669 342
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 968 1573 1821 2843 3822 968
Size: 605 248 1022 979 1285
Fragments arranged by size:
1285 1022 979 605 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 685 717 745 777 811 843 1981 2011 2013
Size: 32 28 32 34 32 1138 30 2
Cuts at: 2013 2043 685
Size: 30 2781
Fragments arranged by size:
2781 1138 34 32 32 32 30 30
28 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 2810 2994 134
Size: 2676 184 1279
Fragments arranged by size:
2676 1279 184
MscI TGG'CCA
Cuts at: 1900 1900
Size: 4139
MslI CAynn'nnrTG
Cuts at: 796 2128 2255 796
Size: 1332 127 2680
Fragments arranged by size:
2680 1332 127
MspA1I CmG'CkG
Cuts at: 1075 1250 1564 1924 2937 3182 1075
Size: 175 314 360 1013 245 2032
Fragments arranged by size:
2032 1013 360 314 245 175
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 1192 1692 2599 1192
Size: 500 907 2732
Fragments arranged by size:
2732 907 500
PasI CC'CwG_GG
Cuts at: 851 851
Size: 4139
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PpuMI rG'GwC_Cy
Cuts at: 222 411 516 222
Size: 189 105 3845
Fragments arranged by size:
3845 189 105
PspOMI G'GGCC_C
Cuts at: 350 895 350
Size: 545 3594
PspXI vC'TCGA_Gb
Cuts at: 325 607 325
Size: 282 3857
PstI C_TGCA'G
Cuts at: 594 1186 1871 594
Size: 592 685 2862
Fragments arranged by size:
2862 685 592
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 1075 1564 1924 1075
Size: 489 360 3290
Fragments arranged by size:
3290 489 360
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 932 1153 932
Size: 221 3918
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 2162 2372 2162
Size: 210 3929
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 590 1182 1867 2860 3051 33
Size: 557 592 685 993 191 1121
Fragments arranged by size:
1121 993 685 592 557 191
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 349 1161 349
Size: 812 3327
SmlI C'TyrA_G
Cuts at: 325 463 607 1294 2209 2701 2963 325
Size: 138 144 687 915 492 262 1501
Fragments arranged by size:
1501 915 687 492 262 144 138
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 1192 1692 1192
Size: 500 3639
StyI C'CwwG_G
Cuts at: 173 500 764 1273 1627 173
Size: 327 264 509 354 2685
Fragments arranged by size:
2685 509 354 327 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 316 808 952 1722 1855 2314 2523 316
Size: 492 144 770 133 459 209 1932
Fragments arranged by size:
1932 770 492 459 209 144 133
TatI w'GTAC_w
Cuts at: 188 3654 188
Size: 3466 673
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3656 3688 3656
Size: 32 4107
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1634 1634
Size: 4139
XhoI C'TCGA_G
Cuts at: 325 607 325
Size: 282 3857
XmaI C'CCGG_G
Cuts at: 347 1159 347
Size: 812 3327
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmlI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI