Restriction map for
pF1KB9925
(Circular) (Six-base) MAPSORT of: pF1KB9925.seq Check: 1749 from: 1 to: 4151
pF1KB9925 4151 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 571 1976 2408 3155 3423 490
Size: 81 1405 432 747 268 1218
Fragments arranged by size:
1405 1218 747 432 268 81
AflIII A'CryG_T
Cuts at: 1016 1503 2607 3584 1016
Size: 487 1104 977 1583
Fragments arranged by size:
1583 1104 977 487
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 886 1456 3023 886
Size: 570 1567 2014
Fragments arranged by size:
2014 1567 570
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 651 785 1155 3504 3760 651
Size: 134 370 2349 256 1042
Fragments arranged by size:
2349 1042 370 256 134
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 119 863 1171 119
Size: 744 308 3099
Fragments arranged by size:
3099 744 308
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 956 1165 2195 956
Size: 209 1030 2912
Fragments arranged by size:
2912 1030 209
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 682 682
Size: 4151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 832 866 832
Size: 34 4117
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 531 1671 531
Size: 1140 3011
BglII A'GATC_T
Cuts at: 286 989 1666 3703 286
Size: 703 677 2037 734
Fragments arranged by size:
2037 734 703 677
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 498 1776 1869 2925 498
Size: 1278 93 1056 1724
Fragments arranged by size:
1724 1278 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 233 1363 1444 1779 3923 233
Size: 1130 81 335 2144 461
Fragments arranged by size:
2144 1130 461 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 4151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1130 970
Size: 160 3991
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 1327 2206 2698 2996 98
Size: 1229 879 492 298 1253
Fragments arranged by size:
1253 1229 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 598 3855 598
Size: 3257 894
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 1582 1830 2532 4028 1582
Size: 248 702 1496 1705
Fragments arranged by size:
1705 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1340 1861 2539 2813 2960 3371 1340
Size: 521 678 274 147 411 2120
Fragments arranged by size:
2120 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1103 1133 1103
Size: 30 4121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 233 1020 202
Size: 31 787 3333
Fragments arranged by size:
3333 787 31
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 680 884 313
Size: 367 204 3580
Fragments arranged by size:
3580 367 204
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 956 1141 1165 1943 2133 2925 956
Size: 185 24 778 190 792 2182
Fragments arranged by size:
2182 792 778 190 185 24
BsmI GAATG_Cn'
Cuts at: 853 853
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 498 956 1141 1165 1776 1869 1943 2133 2195
Size: 458 185 24 611 93 74 190 62
Cuts at: 2195 2925 498
Size: 730 1724
Fragments arranged by size:
1724 730 611 458 190 185 93 74
62 24
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 353 3327 353
Size: 2974 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1201 1415 1717 2098 1201
Size: 214 302 381 3254
Fragments arranged by size:
3254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 971 2063 971
Size: 1092 3059
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 561 1528 561
Size: 967 3184
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstYI r'GATC_y
Cuts at: 1 286 989 1176 1643 1666 2001 2247 3259
Size: 285 703 187 467 23 335 246 1012
Cuts at: 3259 3345 3357 3703 1
Size: 86 12 346 449
Fragments arranged by size:
1012 703 467 449 346 335 285 246
187 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 143 410 498 1394 143
Size: 267 88 896 2900
Fragments arranged by size:
2900 896 267 88
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 1736 1910 2301 2328 3673 1736
Size: 174 391 27 1345 2214
Fragments arranged by size:
2214 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 1062 2174 2384 1062
Size: 1112 210 2829
Fragments arranged by size:
2829 1112 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 954 1163 954
Size: 209 3942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 571 970 1130 1976 2408 3155 3423 490
Size: 81 399 160 846 432 747 268 1218
Fragments arranged by size:
1218 846 747 432 399 268 160 81
EcoO109I rG'GnC_Cy
Cuts at: 1290 1290
Size: 4151
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
EcoRV GAT'ATC
Cuts at: 414 414
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 1585 1833 2855 3834 1585
Size: 248 1022 979 1902
Fragments arranged by size:
1902 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1103 1135 1993 2023 2025 2055 1103
Size: 32 858 30 2 30 3199
Fragments arranged by size:
3199 858 32 30 30 2
HincII GTy'rAC
Cuts at: 935 1190 935
Size: 255 3896
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 908 1021 2140 2267 908
Size: 113 1119 127 2792
Fragments arranged by size:
2792 1119 127 113
MspA1I CmG'CkG
Cuts at: 1262 1576 1936 2949 3194 1262
Size: 314 360 1013 245 2219
Fragments arranged by size:
2219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1020 1204 1704 2611 1020
Size: 184 500 907 2560
Fragments arranged by size:
2560 907 500 184
PciI A'CATG_T
Cuts at: 1016 2607 1016
Size: 1591 2560
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PstI C_TGCA'G
Cuts at: 138 435 493 566 1198 1883 138
Size: 297 58 73 632 685 2406
Fragments arranged by size:
2406 685 632 297 73 58
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 956 1165 956
Size: 209 3942
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 134 210 431 489 562 1194 1879 2872
Size: 101 76 221 58 73 632 685 993
Cuts at: 2872 3063 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 632 221 191 101 76
73 58
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 121 1173 121
Size: 1052 3099
SmlI C'TyrA_G
Cuts at: 113 1306 2221 2713 2975 113
Size: 1193 915 492 262 1289
Fragments arranged by size:
1289 1193 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
SspI AAT'ATT
Cuts at: 892 1048 892
Size: 156 3995
StyI C'CwwG_G
Cuts at: 1285 1639 1285
Size: 354 3797
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 383 1734 1867 2326 2535 383
Size: 1351 133 459 209 1999
Fragments arranged by size:
1999 1351 459 209 133
TatI w'GTAC_w
Cuts at: 206 3666 206
Size: 3460 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 4119
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XmaI C'CCGG_G
Cuts at: 119 1171 119
Size: 1052 3099
XmnI GAAnn'nnTTC
Cuts at: 484 484
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI