Restriction map for
pF1KB9934
(Circular) (Six-base) MAPSORT of: pF1KB9934.seq Check: 7068 from: 1 to: 5054
pF1KB9934 5054 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 702 1297 702
Size: 595 4459
AccI GT'mk_AC
Cuts at: 2092 2092
Size: 5054
Acc65I G'GTAC_C
Cuts at: 2070 2070
Size: 5054
AclI AA'CG_TT
Cuts at: 4894 4894
Size: 5054
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 3311 4058 4326 2879
Size: 432 747 268 3607
Fragments arranged by size:
3607 747 432 268
AfeI AGC'GCT
Cuts at: 376 376
Size: 5054
AflIII A'CryG_T
Cuts at: 435 2406 3510 4487 435
Size: 1971 1104 977 1002
Fragments arranged by size:
1971 1104 1002 977
AgeI A'CCGG_T
Cuts at: 750 3442 750
Size: 2692 2362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1055 1055
Size: 5054
AlwNI CAG_nnn'CTG
Cuts at: 231 492 657 789 1485 1506 2359 3926 231
Size: 261 165 132 696 21 853 1567 1359
Fragments arranged by size:
1567 1359 853 696 261 165 132 21
ApaI G_GGCC'C
Cuts at: 263 263
Size: 5054
ApaLI G'TGCA_C
Cuts at: 3824 3824
Size: 5054
ApoI r'AATT_y
Cuts at: 2058 4407 4663 2058
Size: 2349 256 2449
Fragments arranged by size:
2449 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
AvaI C'yCGr_G
Cuts at: 670 1405 2074 670
Size: 735 669 3650
Fragments arranged by size:
3650 735 669
BamHI G'GATC_C
Cuts at: 2079 2079
Size: 5054
BanI G'GyrC_C
Cuts at: 916 1124 1309 1330 1420 1905 2070 2484 2732
Size: 208 185 21 90 485 165 414 248
Cuts at: 2732 2767 916
Size: 35 3203
Fragments arranged by size:
3203 485 414 248 208 185 165 90
35 21
BanII G_rGCy'C
Cuts at: 263 395 478 1445 1561 1625 2068 3098 263
Size: 132 83 967 116 64 443 1030 2219
Fragments arranged by size:
2219 1030 967 443 132 116 83 64
BbeI G_GCGC'C
Cuts at: 1334 2488 2736 1334
Size: 1154 248 3652
Fragments arranged by size:
3652 1154 248
BbsI GAAGACnn'nnnn_
Cuts at: 158 158
Size: 5054
BciVI GTATCCnnnnn_n'
Cuts at: 1933 2952 3713 5054 1933
Size: 1019 761 1341 1933
Fragments arranged by size:
1933 1341 1019 761
BclI T'GATC_A
Cuts at: 2574 2574
Size: 5054
BglI GCCn_nnn'nGGC
Cuts at: 208 907 208
Size: 699 4355
BglII A'GATC_T
Cuts at: 1235 2569 4606 1235
Size: 1334 2037 1683
Fragments arranged by size:
2037 1683 1334
BlpI GC'TnA_GC
Cuts at: 626 1057 2166 626
Size: 431 1109 3514
Fragments arranged by size:
3514 1109 431
Bme1580I G_kGCm'C
Cuts at: 263 921 1910 2679 2772 3828 263
Size: 658 989 769 93 1056 1489
Fragments arranged by size:
1489 1056 989 769 658 93
BmrI ACTGGGnnnn_n'
Cuts at: 2266 2347 2682 4826 2266
Size: 81 335 2144 2494
Fragments arranged by size:
2494 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1020 1575 3421 1020
Size: 555 1846 2653
Fragments arranged by size:
2653 1846 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 829 1398 1549 1570 1867 2002 157
Size: 672 569 151 21 297 135 3209
Fragments arranged by size:
3209 672 569 297 151 135 21
Bpu10I CC'TnA_GC
Cuts at: 1398 1398
Size: 5054
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 3109 3601 3899 2230
Size: 879 492 298 3385
Fragments arranged by size:
3385 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 133 550 653 929 1185 1424 4758 133
Size: 417 103 276 256 239 3334 429
Fragments arranged by size:
3334 429 417 276 256 239 103
BsaAI yAC'GTr
Cuts at: 582 1599 2013 2407 582
Size: 1017 414 394 3229
Fragments arranged by size:
3229 1017 414 394
BsaBI GATnn'nnATC
Cuts at: 6 2568 4605 6
Size: 2562 2037 455
Fragments arranged by size:
2562 2037 455
BsaHI Gr'CG_yC
Cuts at: 1331 2485 2733 3435 4931 1331
Size: 1154 248 702 1496 1454
Fragments arranged by size:
1496 1454 1154 702 248
BsaWI w'CCGG_w
Cuts at: 750 1451 1925 2243 2764 3442 3716 3863 4274
Size: 701 474 318 521 678 274 147 411
Cuts at: 4274 750
Size: 1530
Fragments arranged by size:
1530 701 678 521 474 411 318 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 595 625 130
Size: 30 435 30 4559
Fragments arranged by size:
4559 435 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 184 1714 1717 1747 1999 2002 2020 126
Size: 58 1530 3 30 252 3 18 3160
Fragments arranged by size:
3160 1530 252 58 30 18 3 3
BseYI C'CCAG_C
Cuts at: 224 742 771 1592 1626 1841 2479 3237 3814
Size: 518 29 821 34 215 638 758 577
Cuts at: 3814 4310 224
Size: 496 968
Fragments arranged by size:
968 821 758 638 577 518 496 215
34 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 114 534 1760 2035 114
Size: 420 1226 275 3133
Fragments arranged by size:
3133 1226 420 275
BsiEI CG_ry'CG
Cuts at: 104 215 431 2596 2642 3850 104
Size: 111 216 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 216 111 46
BsiHKAI G_wGCw'C
Cuts at: 478 1445 1561 2012 2068 2846 3036 3828 478
Size: 967 116 451 56 778 190 792 1704
Fragments arranged by size:
1704 967 792 778 451 190 116 56
BsmBI CGTCTCn'nnnn_
Cuts at: 1822 1822
Size: 5054
Bsp1286I G_dGCh'C
Cuts at: 263 395 478 921 1445 1561 1625 1910 2012
Size: 132 83 443 524 116 64 285 102
Cuts at: 2012 2068 2679 2772 2846 3036 3098 3828 263
Size: 56 611 93 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 524 443 285 190 132
116 102 93 83 74 64 62 56
BspEI T'CCGG_A
Cuts at: 1451 1925 4274 1451
Size: 474 2349 2231
Fragments arranged by size:
2349 2231 474
BspHI T'CATG_A
Cuts at: 4230 4230
Size: 5054
BspMI ACCTGCnnnn'nnnn_
Cuts at: 702 1297 2104 2318 2620 3001 702
Size: 595 807 214 302 381 2755
Fragments arranged by size:
2755 807 595 381 302 214
BsrBI CCG'CTC
Cuts at: 50 116 180 389 630 1737 3346 3400 4884
Size: 66 64 209 241 1107 1609 54 1484
Cuts at: 4884 5049 50
Size: 165 55
Fragments arranged by size:
1609 1484 1107 241 209 165 66 64
55 54
BsrDI GCAATG_nn'
Cuts at: 1352 2966 1352
Size: 1614 3440
BsrFI r'CCGG_y
Cuts at: 750 901 938 3052 3233 3442 750
Size: 151 37 2114 181 209 2362
Fragments arranged by size:
2362 2114 209 181 151 37
BssSI C'ACGA_G
Cuts at: 196 3325 3683 196
Size: 3129 358 1567
Fragments arranged by size:
3129 1567 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2431 2431
Size: 5054
BstBI TT'CG_AA
Cuts at: 3415 3415
Size: 5054
BstXI CCAn_nnnn'nTGG
Cuts at: 1803 1803
Size: 5054
BstYI r'GATC_y
Cuts at: 1 593 1235 2079 2546 2569 2904 3150 4162
Size: 592 642 844 467 23 335 246 1012
Cuts at: 4162 4248 4260 4606 1
Size: 86 12 346 449
Fragments arranged by size:
1012 844 642 592 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 278 278
Size: 5054
BtgI C'CryG_G
Cuts at: 401 555 1434 1577 1910 401
Size: 154 879 143 333 3545
Fragments arranged by size:
3545 879 333 154 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3004 3185 3348 3004
Size: 181 163 4710
Fragments arranged by size:
4710 181 163
BtsI GCAGTG_nn'
Cuts at: 1695 2297 1695
Size: 602 4452
DraI TTT'AAA
Cuts at: 2053 2053
Size: 5054
DraIII CAC_nnn'GTG
Cuts at: 582 582
Size: 5054
DrdI GACnn_nn'nnGTC
Cuts at: 1882 2760 3618 1882
Size: 878 858 3318
Fragments arranged by size:
3318 878 858
EaeI y'GGCC_r
Cuts at: 212 264 428 716 759 864 908 1566 1784
Size: 52 164 288 43 105 44 658 218
Cuts at: 1784 2639 2813 3204 3231 4576 212
Size: 855 174 391 27 1345 690
Fragments arranged by size:
1345 855 690 658 391 288 218 174
164 105 52 44 43 27
EagI C'GGCC_G
Cuts at: 212 428 2639 212
Size: 216 2211 2627
Fragments arranged by size:
2627 2211 216
EarI CTCTTCn'nnn_
Cuts at: 328 1549 3077 3287 328
Size: 1221 1528 210 2095
Fragments arranged by size:
2095 1528 1221 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 210 310 3262 3572 3718 4864 210
Size: 100 2952 310 146 1146 400
Fragments arranged by size:
2952 1146 400 310 146 100
EcoICRI GAG'CTC
Cuts at: 476 1443 1559 2066 476
Size: 967 116 507 3464
Fragments arranged by size:
3464 967 507 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 829 1398 1549 1570 1867 2002 2879 3311
Size: 672 569 151 21 297 135 877 432
Cuts at: 3311 4058 4326 157
Size: 747 268 885
Fragments arranged by size:
885 877 747 672 569 432 297 268
151 135 21
EcoNI CCTnn'n_nnAGG
Cuts at: 910 1033 1304 910
Size: 123 271 4660
Fragments arranged by size:
4660 271 123
EcoO109I rG'GnC_Cy
Cuts at: 654 852 999 1047 1107 1402 1409 2193 654
Size: 198 147 48 60 295 7 784 3515
Fragments arranged by size:
3515 784 295 198 147 60 48 7
EcoRI G'AATT_C
Cuts at: 2058 2058
Size: 5054
EcoRV GAT'ATC
Cuts at: 2028 2028
Size: 5054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 630 3073 630
Size: 2443 2611
FspI TGC'GCA
Cuts at: 2835 2835
Size: 5054
HaeII r_GCGC'y
Cuts at: 186 378 1082 1334 2488 2736 3758 4737 186
Size: 192 704 252 1154 248 1022 979 503
Fragments arranged by size:
1154 1022 979 704 503 252 248 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1358 1390 1446 1478 2896 2926 2928 2958 1358
Size: 32 56 32 1418 30 2 30 3454
Fragments arranged by size:
3454 1418 56 32 32 30 30 2
HincII GTy'rAC
Cuts at: 603 2093 603
Size: 1490 3564
KasI G'GCGC_C
Cuts at: 1330 2484 2732 1330
Size: 1154 248 3652
Fragments arranged by size:
3652 1154 248
KpnI G_GTAC'C
Cuts at: 2074 2074
Size: 5054
MluI A'CGCG_T
Cuts at: 4487 4487
Size: 5054
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3725 3909 3725
Size: 184 4870
MscI TGG'CCA
Cuts at: 761 1568 1786 2815 761
Size: 807 218 1029 3000
Fragments arranged by size:
3000 1029 807 218
MslI CAynn'nnrTG
Cuts at: 164 3043 3170 164
Size: 2879 127 2048
Fragments arranged by size:
2879 2048 127
MspA1I CmG'CkG
Cuts at: 231 403 771 1418 1427 1725 1845 1872 2165
Size: 172 368 647 9 298 120 27 293
Cuts at: 2165 2479 2839 3852 4097 231
Size: 314 360 1013 245 1188
Fragments arranged by size:
1188 1013 647 368 360 314 298 293
245 172 120 27 9
NaeI GCC'GGC
Cuts at: 903 3235 903
Size: 2332 2722
NarI GG'CG_CC
Cuts at: 1331 2485 2733 1331
Size: 1154 248 3652
Fragments arranged by size:
3652 1154 248
NcoI C'CATG_G
Cuts at: 555 555
Size: 5054
NgoMIV G'CCGG_C
Cuts at: 901 3233 901
Size: 2332 2722
NotI GC'GGCC_GC
Cuts at: 212 212
Size: 5054
NspI r_CATG'y
Cuts at: 2107 2607 3514 2107
Size: 500 907 3647
Fragments arranged by size:
3647 907 500
PasI CC'CwG_GG
Cuts at: 874 874
Size: 5054
PciI A'CATG_T
Cuts at: 3510 3510
Size: 5054
PflMI CCAn_nnn'nTGG
Cuts at: 492 714 492
Size: 222 4832
PmeI GTTT'AAAC
Cuts at: 2053 2053
Size: 5054
PmlI CAC'GTG
Cuts at: 582 2013 582
Size: 1431 3623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 5022
PspOMI G'GGCC_C
Cuts at: 259 259
Size: 5054
PstI C_TGCA'G
Cuts at: 362 785 1034 1511 2101 2786 362
Size: 423 249 477 590 685 2630
Fragments arranged by size:
2630 685 590 477 423 249
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5054
PvuII CAG'CTG
Cuts at: 231 1725 1845 1872 2479 2839 231
Size: 1494 120 27 607 360 2446
Fragments arranged by size:
2446 1494 607 360 120 27
RsrII CG'GwC_CG
Cuts at: 3249 3249
Size: 5054
SacI G_AGCT'C
Cuts at: 478 1445 1561 2068 478
Size: 967 116 507 3464
Fragments arranged by size:
3464 967 507 116
SacII CC_GC'GG
Cuts at: 404 404
Size: 5054
SalI G'TCGA_C
Cuts at: 2091 2091
Size: 5054
SapI GCTCTTCn'nnn_
Cuts at: 328 1549 3077 3287 328
Size: 1221 1528 210 2095
Fragments arranged by size:
2095 1528 1221 210
SbfI CC_TGCA'GG
Cuts at: 785 2101 785
Size: 1316 3738
ScaI AGT'ACT
Cuts at: 4571 4571
Size: 5054
SexAI A'CCwGG_T
Cuts at: 563 563
Size: 5054
SfcI C'TryA_G
Cuts at: 33 358 781 1030 1507 1933 2097 2782 3775
Size: 325 423 249 477 426 164 685 993
Cuts at: 3775 3966 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 477 426 423 325 249
191 164
SfoI GGC'GCC
Cuts at: 1332 2486 2734 1332
Size: 1154 248 3652
Fragments arranged by size:
3652 1154 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5054
SmaI CCC'GGG
Cuts at: 1407 2076 1407
Size: 669 4385
SmlI C'TyrA_G
Cuts at: 2209 3124 3616 3878 2209
Size: 915 492 262 3385
Fragments arranged by size:
3385 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5054
SphI G_CATG'C
Cuts at: 2107 2607 2107
Size: 500 4554
StuI AGG'CCT
Cuts at: 124 1852 124
Size: 1728 3326
StyI C'CwwG_G
Cuts at: 530 538 555 1816 2188 2542 530
Size: 8 17 1261 372 354 3042
Fragments arranged by size:
3042 1261 372 354 17 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 1154 2637 2770 3229 3438 568
Size: 586 1483 133 459 209 2184
Fragments arranged by size:
2184 1483 586 459 209 133
TatI w'GTAC_w
Cuts at: 4569 4569
Size: 5054
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 130 162 551 583 4571 4603 130
Size: 32 389 32 3988 32 581
Fragments arranged by size:
3988 581 389 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1157 2851 1157
Size: 1694 3360
XbaI T'CTAG_A
Cuts at: 2085 2085
Size: 5054
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1203 1467 1486 1524 1614 2549 1203
Size: 264 19 38 90 935 3708
Fragments arranged by size:
3708 935 264 90 38 19
XmaI C'CCGG_G
Cuts at: 1405 2074 1405
Size: 669 4385
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI CspCI FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PfoI
PpuMI PshAI PsiI PspXI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI