Restriction map for
pF1KB9936
(Circular) (Six-base) MAPSORT of: pF1KB9936.seq Check: 4897 from: 1 to: 5015
pF1KB9936 5015 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 1868 1868
Size: 5015
AccI GT'mk_AC
Cuts at: 2053 2053
Size: 5015
Acc65I G'GTAC_C
Cuts at: 1356 2031 1356
Size: 675 4340
AclI AA'CG_TT
Cuts at: 4855 4855
Size: 5015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3272 4019 4287 2840
Size: 432 747 268 3568
Fragments arranged by size:
3568 747 432 268
AflIII A'CryG_T
Cuts at: 1565 2367 3471 4448 1565
Size: 802 1104 977 2132
Fragments arranged by size:
2132 1104 977 802
AgeI A'CCGG_T
Cuts at: 3403 3403
Size: 5015
AhdI GACnn_n'nnGTC
Cuts at: 1137 1137
Size: 5015
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1619 1619
Size: 5015
AlwNI CAG_nnn'CTG
Cuts at: 258 1155 1755 2320 3887 258
Size: 897 600 565 1567 1386
Fragments arranged by size:
1567 1386 897 600 565
ApaI G_GGCC'C
Cuts at: 271 271
Size: 5015
ApaLI G'TGCA_C
Cuts at: 631 3785 631
Size: 3154 1861
ApoI r'AATT_y
Cuts at: 133 2019 4368 4624 133
Size: 1886 2349 256 524
Fragments arranged by size:
2349 1886 524 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5015
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
AvaI C'yCGr_G
Cuts at: 366 898 1674 2035 366
Size: 532 776 361 3346
Fragments arranged by size:
3346 776 532 361
BamHI G'GATC_C
Cuts at: 2040 2040
Size: 5015
BanI G'GyrC_C
Cuts at: 328 395 508 982 1269 1356 1428 2031 2445
Size: 67 113 474 287 87 72 603 414
Cuts at: 2445 2693 2728 328
Size: 248 35 2615
Fragments arranged by size:
2615 603 474 414 287 248 113 87
72 67 35
BanII G_rGCy'C
Cuts at: 271 908 1186 1525 1681 2029 3059 271
Size: 637 278 339 156 348 1030 2227
Fragments arranged by size:
2227 1030 637 348 339 278 156
BbeI G_GCGC'C
Cuts at: 986 1273 1432 2449 2697 986
Size: 287 159 1017 248 3304
Fragments arranged by size:
3304 1017 287 248 159
BbsI GAAGACnn'nnnn_
Cuts at: 736 736
Size: 5015
BciVI GTATCCnnnnn_n'
Cuts at: 1753 2913 3674 5015 1753
Size: 1160 761 1341 1753
Fragments arranged by size:
1753 1341 1160 761
BclI T'GATC_A
Cuts at: 2535 2535
Size: 5015
BglI GCCn_nnn'nGGC
Cuts at: 440 506 1034 1597 1721 1775 440
Size: 66 528 563 124 54 3680
Fragments arranged by size:
3680 563 528 124 66 54
BglII A'GATC_T
Cuts at: 2530 4567 2530
Size: 2037 2978
BlpI GC'TnA_GC
Cuts at: 2127 2127
Size: 5015
Bme1580I G_kGCm'C
Cuts at: 271 635 2640 2733 3789 271
Size: 364 2005 93 1056 1497
Fragments arranged by size:
2005 1497 1056 364 93
BmgBI CAC'GTC
Cuts at: 630 630
Size: 5015
BmrI ACTGGGnnnn_n'
Cuts at: 246 274 1368 1765 2227 2308 2643 4787 246
Size: 28 1094 397 462 81 335 2144 474
Fragments arranged by size:
2144 1094 474 462 397 335 81 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3382 3382
Size: 5015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 226 564 915 1165 1641 1642 220
Size: 6 338 351 250 476 1 3593
Fragments arranged by size:
3593 476 351 338 250 6 1
Bpu10I CC'TnA_GC
Cuts at: 211 1054 211
Size: 843 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1891 2191 3070 3562 3860 1891
Size: 300 879 492 298 3046
Fragments arranged by size:
3046 879 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 670 4719 670
Size: 4049 966
BsaAI yAC'GTr
Cuts at: 2368 2368
Size: 5015
BsaBI GATnn'nnATC
Cuts at: 6 1411 1417 2529 4566 6
Size: 1405 6 1112 2037 455
Fragments arranged by size:
2037 1405 1112 455 6
BsaHI Gr'CG_yC
Cuts at: 589 983 1037 1071 1133 1270 1429 1865 2446
Size: 394 54 34 62 137 159 436 581
Cuts at: 2446 2694 3396 4892 589
Size: 248 702 1496 712
Fragments arranged by size:
1496 712 702 581 436 394 248 159
137 62 54 34
BsaWI w'CCGG_w
Cuts at: 1994 2204 2725 3403 3677 3824 4235 1994
Size: 210 521 678 274 147 411 2774
Fragments arranged by size:
2774 678 521 411 274 210 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 856
Size: 30 4985
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 208 916 1002 1214 190
Size: 18 708 86 212 3991
Fragments arranged by size:
3991 708 212 86 18
BseYI C'CCAG_C
Cuts at: 271 1029 1187 1208 1238 1306 1819 1974 2440
Size: 758 158 21 30 68 513 155 466
Cuts at: 2440 3198 3775 4271 271
Size: 758 577 496 1015
Fragments arranged by size:
1015 758 758 577 513 496 466 158
155 68 30 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 556 902 1921 139
Size: 417 346 1019 3233
Fragments arranged by size:
3233 1019 417 346
BsiEI CG_ry'CG
Cuts at: 104 2557 2603 3811 104
Size: 2453 46 1208 1308
Fragments arranged by size:
2453 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 583 635 1003 1060 1525 2029 2807 2997 3789
Size: 52 368 57 465 504 778 190 792
Cuts at: 3789 583
Size: 1809
Fragments arranged by size:
1809 792 778 504 465 368 190 57
52
BsmBI CGTCTCn'nnnn_
Cuts at: 172 172
Size: 5015
Bsp1286I G_dGCh'C
Cuts at: 271 583 635 908 1003 1060 1186 1525 1681
Size: 312 52 273 95 57 126 339 156
Cuts at: 1681 2029 2640 2733 2807 2997 3059 3789 271
Size: 348 611 93 74 190 62 730 1497
Fragments arranged by size:
1497 730 611 348 339 312 273 190
156 126 95 93 74 62 57 52
BspEI T'CCGG_A
Cuts at: 1994 4235 1994
Size: 2241 2774
BspHI T'CATG_A
Cuts at: 200 4191 200
Size: 3991 1024
BspMI ACCTGCnnnn'nnnn_
Cuts at: 266 347 2065 2279 2581 2962 266
Size: 81 1718 214 302 381 2319
Fragments arranged by size:
2319 1718 381 302 214 81
BsrBI CCG'CTC
Cuts at: 50 879 3307 3361 4845 5010 50
Size: 829 2428 54 1484 165 55
Fragments arranged by size:
2428 1484 829 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2927 2927
Size: 5015
BsrFI r'CCGG_y
Cuts at: 434 455 940 985 1431 1459 3013 3194 3403
Size: 21 485 45 446 28 1554 181 209
Cuts at: 3403 434
Size: 2046
Fragments arranged by size:
2046 1554 485 446 209 181 45 28
21
BsrGI T'GTAC_A
Cuts at: 1667 1667
Size: 5015
BssSI C'ACGA_G
Cuts at: 1322 3286 3644 1322
Size: 1964 358 2693
Fragments arranged by size:
2693 1964 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1155 2392 1155
Size: 1237 3778
BstBI TT'CG_AA
Cuts at: 118 3376 118
Size: 3258 1757
BstXI CCAn_nnnn'nTGG
Cuts at: 289 1848 289
Size: 1559 3456
BstYI r'GATC_y
Cuts at: 1 2040 2507 2530 2865 3111 4123 4209 4221
Size: 2039 467 23 335 246 1012 86 12
Cuts at: 4221 4567 1
Size: 346 449
Fragments arranged by size:
2039 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 127 332 399 530 1127 1728 107
Size: 20 205 67 131 597 601 3394
Fragments arranged by size:
3394 601 597 205 131 67 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1067 1404 2965 3146 3309 1067
Size: 337 1561 181 163 2773
Fragments arranged by size:
2773 1561 337 181 163
BtsI GCAGTG_nn'
Cuts at: 621 2258 621
Size: 1637 3378
ClaI AT'CG_AT
Cuts at: 1405 1405
Size: 5015
DraI TTT'AAA
Cuts at: 2014 2014
Size: 5015
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2721 3579 1809
Size: 912 858 3245
Fragments arranged by size:
3245 912 858
EaeI y'GGCC_r
Cuts at: 432 953 987 1395 1469 1589 1703 1850 1967
Size: 521 34 408 74 120 114 147 117
Cuts at: 1967 2600 2774 3165 3192 4537 432
Size: 633 174 391 27 1345 910
Fragments arranged by size:
1345 910 633 521 408 391 174 147
120 117 114 74 34 27
EagI C'GGCC_G
Cuts at: 2600 2600
Size: 5015
EarI CTCTTCn'nnn_
Cuts at: 668 3038 3248 668
Size: 2370 210 2435
Fragments arranged by size:
2435 2370 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 555 1267 3223 3533 3679 4825 187
Size: 368 712 1956 310 146 1146 377
Fragments arranged by size:
1956 1146 712 377 368 310 146
EcoICRI GAG'CTC
Cuts at: 1523 2027 1523
Size: 504 4511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 226 564 915 1165 1641 1642 2840 3272
Size: 6 338 351 250 476 1 1198 432
Cuts at: 3272 4019 4287 220
Size: 747 268 948
Fragments arranged by size:
1198 948 747 476 432 351 338 268
250 6 1
EcoO109I rG'GnC_Cy
Cuts at: 207 268 296 620 759 839 2154 207
Size: 61 28 324 139 80 1315 3068
Fragments arranged by size:
3068 1315 324 139 80 61 28
EcoRI G'AATT_C
Cuts at: 2019 2019
Size: 5015
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 5015
FspI TGC'GCA
Cuts at: 2796 2796
Size: 5015
HaeII r_GCGC'y
Cuts at: 986 1273 1313 1432 1460 2449 2697 3719 4698
Size: 287 40 119 28 989 248 1022 979
Cuts at: 4698 986
Size: 1303
Fragments arranged by size:
1303 1022 989 979 287 248 119 40
28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 1123 1155 1408 1440 1830 1862 2857
Size: 32 424 32 253 32 390 32 995
Cuts at: 2857 2887 2889 2919 667
Size: 30 2 30 2763
Fragments arranged by size:
2763 995 424 390 253 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 656 2054 656
Size: 1398 3617
HpaI GTT'AAC
Cuts at: 656 656
Size: 5015
KasI G'GCGC_C
Cuts at: 982 1269 1428 2445 2693 982
Size: 287 159 1017 248 3304
Fragments arranged by size:
3304 1017 287 248 159
KpnI G_GTAC'C
Cuts at: 1360 2035 1360
Size: 675 4340
MluI A'CGCG_T
Cuts at: 1565 4448 1565
Size: 2883 2132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1904 3686 3870 1904
Size: 1782 184 3049
Fragments arranged by size:
3049 1782 184
MscI TGG'CCA
Cuts at: 1397 2776 1397
Size: 1379 3636
MslI CAynn'nnrTG
Cuts at: 3004 3131 3004
Size: 127 4888
MspA1I CmG'CkG
Cuts at: 129 497 532 542 891 1033 1101 1155 1620
Size: 368 35 10 349 142 68 54 465
Cuts at: 1620 2126 2440 2800 3813 4058 129
Size: 506 314 360 1013 245 1086
Fragments arranged by size:
1086 1013 506 465 368 360 349 314
245 142 68 54 35 10
NaeI GCC'GGC
Cuts at: 436 457 987 1433 1461 3196 436
Size: 21 530 446 28 1735 2255
Fragments arranged by size:
2255 1735 530 446 28 21
NarI GG'CG_CC
Cuts at: 983 1270 1429 2446 2694 983
Size: 287 159 1017 248 3304
Fragments arranged by size:
3304 1017 287 248 159
NcoI C'CATG_G
Cuts at: 107 332 399 1127 107
Size: 225 67 728 3995
Fragments arranged by size:
3995 728 225 67
NgoMIV G'CCGG_C
Cuts at: 434 455 985 1431 1459 3194 434
Size: 21 530 446 28 1735 2255
Fragments arranged by size:
2255 1735 530 446 28 21
NspI r_CATG'y
Cuts at: 1944 2068 2568 3475 1944
Size: 124 500 907 3484
Fragments arranged by size:
3484 907 500 124
PasI CC'CwG_GG
Cuts at: 1516 1545 1516
Size: 29 4986
PciI A'CATG_T
Cuts at: 3471 3471
Size: 5015
PflMI CCAn_nnn'nTGG
Cuts at: 405 1620 405
Size: 1215 3800
PmeI GTTT'AAAC
Cuts at: 2014 2014
Size: 5015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4983
PpuMI rG'GwC_Cy
Cuts at: 207 296 620 759 839 207
Size: 89 324 139 80 4383
Fragments arranged by size:
4383 324 139 89 80
PspOMI G'GGCC_C
Cuts at: 267 267
Size: 5015
PstI C_TGCA'G
Cuts at: 1442 2062 2747 1442
Size: 620 685 3710
Fragments arranged by size:
3710 685 620
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5015
PvuII CAG'CTG
Cuts at: 497 542 1033 1155 1620 2440 2800 497
Size: 45 491 122 465 820 360 2712
Fragments arranged by size:
2712 820 491 465 360 122 45
RsrII CG'GwC_CG
Cuts at: 3210 3210
Size: 5015
SacI G_AGCT'C
Cuts at: 1525 2029 1525
Size: 504 4511
SacII CC_GC'GG
Cuts at: 130 533 130
Size: 403 4612
SalI G'TCGA_C
Cuts at: 2052 2052
Size: 5015
SanDI GG'GwC_CC
Cuts at: 296 620 839 296
Size: 324 219 4472
Fragments arranged by size:
4472 324 219
SapI GCTCTTCn'nnn_
Cuts at: 3038 3248 3038
Size: 210 4805
SbfI CC_TGCA'GG
Cuts at: 2062 2062
Size: 5015
ScaI AGT'ACT
Cuts at: 286 4532 286
Size: 4246 769
SfcI C'TryA_G
Cuts at: 33 803 1121 1438 2058 2743 3736 3927 33
Size: 770 318 317 620 685 993 191 1121
Fragments arranged by size:
1121 993 770 685 620 318 317 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1597 1597
Size: 5015
SfoI GGC'GCC
Cuts at: 984 1271 1430 2447 2695 984
Size: 287 159 1017 248 3304
Fragments arranged by size:
3304 1017 287 248 159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5015
SmaI CCC'GGG
Cuts at: 1676 2037 1676
Size: 361 4654
SmlI C'TyrA_G
Cuts at: 1906 2170 3085 3577 3839 1906
Size: 264 915 492 262 3082
Fragments arranged by size:
3082 915 492 264 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5015
SphI G_CATG'C
Cuts at: 2068 2568 2068
Size: 500 4515
SrfI GCCC'GGGC
Cuts at: 1676 1676
Size: 5015
StyI C'CwwG_G
Cuts at: 107 332 399 1127 2149 2503 107
Size: 225 67 728 1022 354 2619
Fragments arranged by size:
2619 1022 728 354 225 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 838 1122 1189 2598 2731 3190 3399 838
Size: 284 67 1409 133 459 209 2454
Fragments arranged by size:
2454 1409 459 284 209 133 67
TatI w'GTAC_w
Cuts at: 284 1667 4530 284
Size: 1383 2863 769
Fragments arranged by size:
2863 1383 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 4532 4564 856
Size: 32 3644 32 1307
Fragments arranged by size:
3644 1307 32 32
Tth111I GACn'n_nGTC
Cuts at: 735 2812 735
Size: 2077 2938
XbaI T'CTAG_A
Cuts at: 2046 2046
Size: 5015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 322 2510 322
Size: 2188 2827
XmaI C'CCGG_G
Cuts at: 1674 2035 1674
Size: 361 4654
ZraI GAC'GTC
Cuts at: 1866 1866
Size: 5015
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI BsiWI BsmI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SexAI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI