Restriction map for
pF1KB9941
(Circular) (Six-base) MAPSORT of: pF1KB9941.seq Check: 1773 from: 1 to: 4700
pF1KB9941 4700 bp
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4700
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 458 551 1006 2525 2957 3704 3972 165
Size: 293 93 455 1519 432 747 268 893
Fragments arranged by size:
1519 893 747 455 432 293 268 93
AflIII A'CryG_T
Cuts at: 1427 2052 3156 4133 1427
Size: 625 1104 977 1994
Fragments arranged by size:
1994 1104 977 625
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AhdI GACnn_n'nnGTC
Cuts at: 1431 1592 1431
Size: 161 4539
AleI CACnn'nnGTG
Cuts at: 967 967
Size: 4700
AlwNI CAG_nnn'CTG
Cuts at: 771 852 1393 2005 3572 771
Size: 81 541 612 1567 1899
Fragments arranged by size:
1899 1567 612 541 81
ApaLI G'TGCA_C
Cuts at: 3470 3470
Size: 4700
ApoI r'AATT_y
Cuts at: 1704 4053 4309 1704
Size: 2349 256 2095
Fragments arranged by size:
2349 2095 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 1681 1720 1681
Size: 39 4661
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1657 1690 1657
Size: 33 4667
BamHI G'GATC_C
Cuts at: 1442 1725 1442
Size: 283 4417
BanI G'GyrC_C
Cuts at: 313 640 916 994 1716 2130 2378 2413 313
Size: 327 276 78 722 414 248 35 2600
Fragments arranged by size:
2600 722 414 327 276 248 78 35
BanII G_rGCy'C
Cuts at: 1094 1460 1714 2744 1094
Size: 366 254 1030 3050
Fragments arranged by size:
3050 1030 366 254
BbeI G_GCGC'C
Cuts at: 2134 2382 2134
Size: 248 4452
BbsI GAAGACnn'nnnn_
Cuts at: 985 985
Size: 4700
BbvCI CC'TCA_GC
Cuts at: 1208 1208
Size: 4700
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3359 4700 2598
Size: 761 1341 2598
Fragments arranged by size:
2598 1341 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglI GCCn_nnn'nGGC
Cuts at: 914 1550 914
Size: 636 4064
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 1812 1812
Size: 4700
Bme1580I G_kGCm'C
Cuts at: 919 949 997 1546 2325 2418 3474 919
Size: 30 48 549 779 93 1056 2145
Fragments arranged by size:
2145 1056 779 549 93 48 30
BmgBI CAC'GTC
Cuts at: 1428 1428
Size: 4700
BmrI ACTGGGnnnn_n'
Cuts at: 675 1912 1993 2328 4472 675
Size: 1237 81 335 2144 903
Fragments arranged by size:
2144 1237 903 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1039 1532 1663 922
Size: 117 493 131 3959
Fragments arranged by size:
3959 493 131 117
Bpu10I CC'TnA_GC
Cuts at: 1208 1208
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 1876 2755 3247 3545 1129
Size: 747 879 492 298 2284
Fragments arranged by size:
2284 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 467 622 4404 467
Size: 155 3782 763
Fragments arranged by size:
3782 763 155
BsaAI yAC'GTr
Cuts at: 504 950 2053 504
Size: 446 1103 3151
Fragments arranged by size:
3151 1103 446
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 869 1593 2131 2379 3081 4577 869
Size: 724 538 248 702 1496 992
Fragments arranged by size:
1496 992 724 702 538 248
BsaWI w'CCGG_w
Cuts at: 472 1889 2410 3088 3362 3509 3920 472
Size: 1417 521 678 274 147 411 1252
Fragments arranged by size:
1417 1252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 445 475 1642 1672 445
Size: 30 1167 30 3473
Fragments arranged by size:
3473 1167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 1036 1102 1621 267
Size: 769 66 519 3346
Fragments arranged by size:
3346 769 519 66
BseYI C'CCAG_C
Cuts at: 493 852 912 942 1557 2125 2883 3460 3956
Size: 359 60 30 615 568 758 577 496
Cuts at: 3956 493
Size: 1237
Fragments arranged by size:
1237 758 615 577 568 496 359 60
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 796 813 1084 784
Size: 12 17 271 4400
Fragments arranged by size:
4400 271 17 12
BsiEI CG_ry'CG
Cuts at: 104 2242 2288 3496 104
Size: 2138 46 1208 1308
Fragments arranged by size:
2138 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1460 1714 2492 2682 3474 1460
Size: 254 778 190 792 2686
Fragments arranged by size:
2686 792 778 254 190
BsmI GAATG_Cn'
Cuts at: 488 488
Size: 4700
BsmBI CGTCTCn'nnnn_
Cuts at: 1327 1327
Size: 4700
Bsp1286I G_dGCh'C
Cuts at: 919 949 997 1094 1460 1546 1714 2325 2418
Size: 30 48 97 366 86 168 611 93
Cuts at: 2418 2492 2682 2744 3474 919
Size: 74 190 62 730 2145
Fragments arranged by size:
2145 730 611 366 190 168 97 93
86 74 62 48 30
BspEI T'CCGG_A
Cuts at: 472 3920 472
Size: 3448 1252
BspHI T'CATG_A
Cuts at: 836 1034 3876 836
Size: 198 2842 1660
Fragments arranged by size:
2842 1660 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 821 1020 1637 1750 1964 2266 2647 821
Size: 199 617 113 214 302 381 2874
Fragments arranged by size:
2874 617 381 302 214 199 113
BsrBI CCG'CTC
Cuts at: 50 1284 2992 3046 4530 4695 50
Size: 1234 1708 54 1484 165 55
Fragments arranged by size:
1708 1484 1234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 353 2612 353
Size: 2259 2441
BsrFI r'CCGG_y
Cuts at: 138 908 919 2698 2879 3088 138
Size: 770 11 1779 181 209 1750
Fragments arranged by size:
1779 1750 770 209 181 11
BssSI C'ACGA_G
Cuts at: 2971 3329 2971
Size: 358 4342
BstAPI GCAn_nnn'nTGC
Cuts at: 923 2077 923
Size: 1154 3546
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstEII G'GTnAC_C
Cuts at: 342 342
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 900 1272 900
Size: 372 4328
BstYI r'GATC_y
Cuts at: 1 704 1442 1725 2192 2215 2550 2796 3808
Size: 703 738 283 467 23 335 246 1012
Cuts at: 3808 3894 3906 4252 1
Size: 86 12 346 449
Fragments arranged by size:
1012 738 703 467 449 346 335 283
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1255 1255
Size: 4700
BtgI C'CryG_G
Cuts at: 107 146 1076 1577 107
Size: 39 930 501 3230
Fragments arranged by size:
3230 930 501 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 804 2650 2831 2994 804
Size: 1846 181 163 2510
Fragments arranged by size:
2510 1846 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 1943
Size: 4700
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 2406 3264 2406
Size: 858 3842
EaeI y'GGCC_r
Cuts at: 143 149 860 2285 2459 2850 2877 4222 143
Size: 6 711 1425 174 391 27 1345 621
Fragments arranged by size:
1425 1345 711 621 391 174 27 6
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 527 2723 2933 527
Size: 2196 210 2294
Fragments arranged by size:
2294 2196 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2908 3218 3364 4510 2908
Size: 310 146 1146 3098
Fragments arranged by size:
3098 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1458 1712 1458
Size: 254 4446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 458 551 922 1006 1039 1532 1663 2525
Size: 293 93 371 84 33 493 131 862
Cuts at: 2525 2957 3704 3972 165
Size: 432 747 268 893
Fragments arranged by size:
893 862 747 493 432 371 293 268
131 93 84 33
EcoNI CCTnn'n_nnAGG
Cuts at: 862 1021 1570 862
Size: 159 549 3992
Fragments arranged by size:
3992 549 159
EcoO109I rG'GnC_Cy
Cuts at: 605 1839 605
Size: 1234 3466
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4700
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 909 2134 2382 3404 4383 909
Size: 1225 248 1022 979 1226
Fragments arranged by size:
1226 1225 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 1400 1417 1432 1449 1578 1610 2542
Size: 32 923 17 15 17 129 32 932
Cuts at: 2542 2572 2574 2604 445
Size: 30 2 30 2541
Fragments arranged by size:
2541 932 923 129 32 32 30 30
17 17 15 2
HincII GTy'rAC
Cuts at: 516 1739 516
Size: 1223 3477
KasI G'GCGC_C
Cuts at: 2130 2378 2130
Size: 248 4452
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MfeI C'AATT_G
Cuts at: 1180 1180
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1490 3371 3555 1064
Size: 426 1881 184 2209
Fragments arranged by size:
2209 1881 426 184
MscI TGG'CCA
Cuts at: 145 862 2461 145
Size: 717 1599 2384
Fragments arranged by size:
2384 1599 717
MslI CAynn'nnrTG
Cuts at: 790 835 967 2689 2816 790
Size: 45 132 1722 127 2674
Fragments arranged by size:
2674 1722 132 127 45
MspA1I CmG'CkG
Cuts at: 579 1068 1663 1811 2125 2485 3498 3743 579
Size: 489 595 148 314 360 1013 245 1536
Fragments arranged by size:
1536 1013 595 489 360 314 245 148
NaeI GCC'GGC
Cuts at: 910 2881 910
Size: 1971 2729
NarI GG'CG_CC
Cuts at: 2131 2379 2131
Size: 248 4452
NcoI C'CATG_G
Cuts at: 107 1577 107
Size: 1470 3230
NgoMIV G'CCGG_C
Cuts at: 908 2879 908
Size: 1971 2729
NspI r_CATG'y
Cuts at: 1539 1753 2253 3160 1539
Size: 214 500 907 3079
Fragments arranged by size:
3079 907 500 214
PasI CC'CwG_GG
Cuts at: 600 600
Size: 4700
PciI A'CATG_T
Cuts at: 3156 3156
Size: 4700
PflMI CCAn_nnn'nTGG
Cuts at: 1185 1376 1185
Size: 191 4509
PfoI T'CCnGG_A
Cuts at: 659 1016 1640 659
Size: 357 624 3719
Fragments arranged by size:
3719 624 357
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PmlI CAC'GTG
Cuts at: 950 950
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4668
PstI C_TGCA'G
Cuts at: 1634 1747 2432 1634
Size: 113 685 3902
Fragments arranged by size:
3902 685 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 579 1068 2125 2485 579
Size: 489 1057 360 2794
Fragments arranged by size:
2794 1057 489 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1460 1714 1460
Size: 254 4446
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 4217 4217
Size: 4700
SfcI C'TryA_G
Cuts at: 33 264 1630 1743 2428 3421 3612 33
Size: 231 1366 113 685 993 191 1121
Fragments arranged by size:
1366 1121 993 685 231 191 113
SfoI GGC'GCC
Cuts at: 2132 2380 2132
Size: 248 4452
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1683 1722 1683
Size: 39 4661
SmlI C'TyrA_G
Cuts at: 1144 1855 2770 3262 3524 1144
Size: 711 915 492 262 2320
Fragments arranged by size:
2320 915 711 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 1753 2253 1753
Size: 500 4200
StuI AGG'CCT
Cuts at: 1012 1614 1012
Size: 602 4098
StyI C'CwwG_G
Cuts at: 107 152 404 863 1353 1466 1577 1834 2188
Size: 45 252 459 490 113 111 257 354
Cuts at: 2188 107
Size: 2619
Fragments arranged by size:
2619 490 459 354 257 252 113 111
45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 449 1123 2283 2416 2875 3084 449
Size: 674 1160 133 459 209 2065
Fragments arranged by size:
2065 1160 674 459 209 133
TatI w'GTAC_w
Cuts at: 1340 4215 1340
Size: 2875 1825
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 2497 2497
Size: 4700
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 2195 501
Size: 1694 3006
XmaI C'CCGG_G
Cuts at: 1681 1720 1681
Size: 39 4661
XmnI GAAnn'nnTTC
Cuts at: 1012 1012
Size: 4700
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BcgI BfrBI BmtI BsiWI BsrGI BssHII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI