Restriction map for
pF1KB9947
(Circular) (Six-base) MAPSORT of: pF1KB9947.seq Check: 1233 from: 1 to: 4706
pF1KB9947 4706 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1744 1744
Size: 4706
Acc65I G'GTAC_C
Cuts at: 1722 1722
Size: 4706
AclI AA'CG_TT
Cuts at: 4546 4546
Size: 4706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 814 1157 2531 2963 3710 3978 529
Size: 285 343 1374 432 747 268 1257
Fragments arranged by size:
1374 1257 747 432 343 285 268
AflIII A'CryG_T
Cuts at: 194 2058 3162 4139 194
Size: 1864 1104 977 761
Fragments arranged by size:
1864 1104 977 761
AgeI A'CCGG_T
Cuts at: 3094 3094
Size: 4706
AhdI GACnn_n'nnGTC
Cuts at: 1487 1487
Size: 4706
AleI CACnn'nnGTG
Cuts at: 1345 1345
Size: 4706
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 689 1265 689
Size: 576 4130
AlwNI CAG_nnn'CTG
Cuts at: 159 792 1098 1245 1267 2011 3578 159
Size: 633 306 147 22 744 1567 1287
Fragments arranged by size:
1567 1287 744 633 306 147 22
ApaLI G'TGCA_C
Cuts at: 3476 3476
Size: 4706
ApoI r'AATT_y
Cuts at: 1710 4059 4315 1710
Size: 2349 256 2101
Fragments arranged by size:
2349 2101 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4706
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
AvaI C'yCGr_G
Cuts at: 1130 1604 1726 1130
Size: 474 122 4110
Fragments arranged by size:
4110 474 122
BamHI G'GATC_C
Cuts at: 1731 1731
Size: 4706
BanI G'GyrC_C
Cuts at: 673 1311 1351 1652 1722 2136 2384 2419 673
Size: 638 40 301 70 414 248 35 2960
Fragments arranged by size:
2960 638 414 301 248 70 40 35
BanII G_rGCy'C
Cuts at: 476 1720 2750 476
Size: 1244 1030 2432
Fragments arranged by size:
2432 1244 1030
BbeI G_GCGC'C
Cuts at: 1315 1656 2140 2388 1315
Size: 341 484 248 3633
Fragments arranged by size:
3633 484 341 248
BbsI GAAGACnn'nnnn_
Cuts at: 1017 1017
Size: 4706
BbvCI CC'TCA_GC
Cuts at: 1556 1556
Size: 4706
BciVI GTATCCnnnnn_n'
Cuts at: 2604 3365 4706 2604
Size: 761 1341 2604
Fragments arranged by size:
2604 1341 761
BclI T'GATC_A
Cuts at: 1109 2226 1109
Size: 1117 3589
BglI GCCn_nnn'nGGC
Cuts at: 1258 1360 1258
Size: 102 4604
BglII A'GATC_T
Cuts at: 626 2221 4258 626
Size: 1595 2037 1074
Fragments arranged by size:
2037 1595 1074
BlpI GC'TnA_GC
Cuts at: 1818 1818
Size: 4706
Bme1580I G_kGCm'C
Cuts at: 1452 2331 2424 3480 1452
Size: 879 93 1056 2678
Fragments arranged by size:
2678 1056 879 93
BmgBI CAC'GTC
Cuts at: 1563 1563
Size: 4706
BmrI ACTGGGnnnn_n'
Cuts at: 479 1531 1918 1999 2334 4478 479
Size: 1052 387 81 335 2144 707
Fragments arranged by size:
2144 1052 707 387 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 3073 541
Size: 2532 2174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 640 979 1120 1214 1243 208
Size: 432 339 141 94 29 3671
Fragments arranged by size:
3671 432 339 141 94 29
Bpu10I CC'TnA_GC
Cuts at: 1556 1556
Size: 4706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1882 2761 3253 3551 1882
Size: 879 492 298 3037
Fragments arranged by size:
3037 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 358 4410 358
Size: 4052 654
BsaAI yAC'GTr
Cuts at: 633 1447 2059 633
Size: 814 612 3280
Fragments arranged by size:
3280 814 612
BsaBI GATnn'nnATC
Cuts at: 6 2220 4257 6
Size: 2214 2037 455
Fragments arranged by size:
2214 2037 455
BsaHI Gr'CG_yC
Cuts at: 1312 1653 2137 2385 3087 4583 1312
Size: 341 484 248 702 1496 1435
Fragments arranged by size:
1496 1435 702 484 341 248
BsaWI w'CCGG_w
Cuts at: 1645 1895 2416 3094 3368 3515 3926 1645
Size: 250 521 678 274 147 411 2425
Fragments arranged by size:
2425 678 521 411 274 250 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 1616 1646 181
Size: 30 1405 30 3241
Fragments arranged by size:
3241 1405 30 30
BseYI C'CCAG_C
Cuts at: 152 264 1066 2131 2889 3466 3962 152
Size: 112 802 1065 758 577 496 896
Fragments arranged by size:
1065 896 802 758 577 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 145
Size: 4706
BsiEI CG_ry'CG
Cuts at: 104 2248 2294 3502 104
Size: 2144 46 1208 1308
Fragments arranged by size:
2144 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 194 1720 2498 2688 3480 194
Size: 1526 778 190 792 1420
Fragments arranged by size:
1526 1420 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 277 277
Size: 4706
Bsp1286I G_dGCh'C
Cuts at: 194 476 1452 1720 2331 2424 2498 2688 2750
Size: 282 976 268 611 93 74 190 62
Cuts at: 2750 3480 194
Size: 730 1420
Fragments arranged by size:
1420 976 730 611 282 268 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3926 3926
Size: 4706
BspHI T'CATG_A
Cuts at: 348 927 3882 348
Size: 579 2955 1172
Fragments arranged by size:
2955 1172 579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1403 1756 1970 2272 2653 1403
Size: 353 214 302 381 3456
Fragments arranged by size:
3456 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 2998 3052 4536 4701 50
Size: 2948 54 1484 165 55
Fragments arranged by size:
2948 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1457 2618 1457
Size: 1161 3545
BsrFI r'CCGG_y
Cuts at: 422 1698 2704 2885 3094 422
Size: 1276 1006 181 209 2034
Fragments arranged by size:
2034 1276 1006 209 181
BssSI C'ACGA_G
Cuts at: 986 2977 3335 986
Size: 1991 358 2357
Fragments arranged by size:
2357 1991 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1267 2083 1267
Size: 816 3890
BstBI TT'CG_AA
Cuts at: 3067 3067
Size: 4706
BstXI CCAn_nnnn'nTGG
Cuts at: 1347 1347
Size: 4706
BstYI r'GATC_y
Cuts at: 1 461 626 789 1731 2198 2221 2556 2802
Size: 460 165 163 942 467 23 335 246
Cuts at: 2802 3814 3900 3912 4258 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 942 467 460 449 346 335 246
165 163 86 23 12
BtgI C'CryG_G
Cuts at: 170 389 1346 1355 1506 170
Size: 219 957 9 151 3370
Fragments arranged by size:
3370 957 219 151 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2656 2837 3000 2656
Size: 181 163 4362
Fragments arranged by size:
4362 181 163
BtsI GCAGTG_nn'
Cuts at: 491 1149 1386 1949 491
Size: 658 237 563 3248
Fragments arranged by size:
3248 658 563 237
DraI TTT'AAA
Cuts at: 1705 1705
Size: 4706
DraIII CAC_nnn'GTG
Cuts at: 1428 1428
Size: 4706
DrdI GACnn_nn'nnGTC
Cuts at: 2412 3270 2412
Size: 858 3848
EaeI y'GGCC_r
Cuts at: 1151 1430 2291 2465 2856 2883 4228 1151
Size: 279 861 174 391 27 1345 1629
Fragments arranged by size:
1629 1345 861 391 279 174 27
EagI C'GGCC_G
Cuts at: 2291 2291
Size: 4706
EarI CTCTTCn'nnn_
Cuts at: 2729 2939 2729
Size: 210 4496
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1291 2914 3224 3370 4516 1291
Size: 1623 310 146 1146 1481
Fragments arranged by size:
1623 1481 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1718 1718
Size: 4706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 529 640 814 979 1120 1157 1214 1243
Size: 321 111 174 165 141 37 57 29
Cuts at: 1243 2531 2963 3710 3978 208
Size: 1288 432 747 268 936
Fragments arranged by size:
1288 936 747 432 321 268 174 165
141 111 57 37 29
EcoO109I rG'GnC_Cy
Cuts at: 454 876 1533 1845 454
Size: 422 657 312 3315
Fragments arranged by size:
3315 657 422 312
EcoRI G'AATT_C
Cuts at: 1710 1710
Size: 4706
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 419 2725 419
Size: 2306 2400
FspI TGC'GCA
Cuts at: 382 1234 2487 382
Size: 852 1253 2601
Fragments arranged by size:
2601 1253 852
FspAI rTGC'GCAy
Cuts at: 382 1234 382
Size: 852 3854
HaeII r_GCGC'y
Cuts at: 1315 1656 2140 2388 3410 4389 1315
Size: 341 484 248 1022 979 1632
Fragments arranged by size:
1632 1022 979 484 341 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 446 478 1458 1473 1490 1505 1616 1648 2548
Size: 32 980 15 17 15 111 32 900
Cuts at: 2548 2578 2580 2610 446
Size: 30 2 30 2542
Fragments arranged by size:
2542 980 900 111 32 32 30 30
17 15 15 2
HincII GTy'rAC
Cuts at: 1745 1745
Size: 4706
HindIII A'AGCT_T
Cuts at: 396 396
Size: 4706
KasI G'GCGC_C
Cuts at: 1311 1652 2136 2384 1311
Size: 341 484 248 3633
Fragments arranged by size:
3633 484 341 248
KpnI G_GTAC'C
Cuts at: 1726 1726
Size: 4706
MluI A'CGCG_T
Cuts at: 4139 4139
Size: 4706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 752 3377 3561 752
Size: 2625 184 1897
Fragments arranged by size:
2625 1897 184
MscI TGG'CCA
Cuts at: 1153 1432 2467 1153
Size: 279 1035 3392
Fragments arranged by size:
3392 1035 279
MslI CAynn'nnrTG
Cuts at: 1345 1462 2695 2822 1345
Size: 117 1233 127 3229
Fragments arranged by size:
3229 1233 127 117
MspA1I CmG'CkG
Cuts at: 159 488 1817 2131 2491 3504 3749 159
Size: 329 1329 314 360 1013 245 1116
Fragments arranged by size:
1329 1116 1013 360 329 314 245
NaeI GCC'GGC
Cuts at: 2887 2887
Size: 4706
NarI GG'CG_CC
Cuts at: 1312 1653 2137 2385 1312
Size: 341 484 248 3633
Fragments arranged by size:
3633 484 341 248
NcoI C'CATG_G
Cuts at: 170 170
Size: 4706
NgoMIV G'CCGG_C
Cuts at: 2885 2885
Size: 4706
NspI r_CATG'y
Cuts at: 198 1239 1759 2259 3166 198
Size: 1041 520 500 907 1738
Fragments arranged by size:
1738 1041 907 520 500
PciI A'CATG_T
Cuts at: 194 3162 194
Size: 2968 1738
PfoI T'CCnGG_A
Cuts at: 185 785 956 1163 1541 185
Size: 600 171 207 378 3350
Fragments arranged by size:
3350 600 378 207 171
PmeI GTTT'AAAC
Cuts at: 1705 1705
Size: 4706
PmlI CAC'GTG
Cuts at: 1447 1447
Size: 4706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 4674
PpuMI rG'GwC_Cy
Cuts at: 454 876 1533 454
Size: 422 657 3627
Fragments arranged by size:
3627 657 422
PstI C_TGCA'G
Cuts at: 498 1017 1381 1753 2438 498
Size: 519 364 372 685 2766
Fragments arranged by size:
2766 685 519 372 364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4706
PvuII CAG'CTG
Cuts at: 159 2131 2491 159
Size: 1972 360 2374
Fragments arranged by size:
2374 1972 360
RsrII CG'GwC_CG
Cuts at: 2901 2901
Size: 4706
SacI G_AGCT'C
Cuts at: 1720 1720
Size: 4706
SalI G'TCGA_C
Cuts at: 1743 1743
Size: 4706
SapI GCTCTTCn'nnn_
Cuts at: 2729 2939 2729
Size: 210 4496
SbfI CC_TGCA'GG
Cuts at: 1753 1753
Size: 4706
ScaI AGT'ACT
Cuts at: 4223 4223
Size: 4706
SexAI A'CCwGG_T
Cuts at: 1424 1424
Size: 4706
SfcI C'TryA_G
Cuts at: 33 494 1013 1377 1749 2434 3427 3618 33
Size: 461 519 364 372 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 461 372 364 191
SfoI GGC'GCC
Cuts at: 1313 1654 2138 2386 1313
Size: 341 484 248 3633
Fragments arranged by size:
3633 484 341 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4706
SmaI CCC'GGG
Cuts at: 1728 1728
Size: 4706
SmlI C'TyrA_G
Cuts at: 1861 2776 3268 3530 1861
Size: 915 492 262 3037
Fragments arranged by size:
3037 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4706
SphI G_CATG'C
Cuts at: 1239 1759 2259 1239
Size: 520 500 3686
Fragments arranged by size:
3686 520 500
StuI AGG'CCT
Cuts at: 1363 1549 1363
Size: 186 4520
StyI C'CwwG_G
Cuts at: 170 713 1840 2194 170
Size: 543 1127 354 2682
Fragments arranged by size:
2682 1127 543 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 237 1339 2289 2422 2881 3090 237
Size: 1102 950 133 459 209 1853
Fragments arranged by size:
1853 1102 950 459 209 133
TatI w'GTAC_w
Cuts at: 4221 4221
Size: 4706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 302 334 4223 4255 181
Size: 32 89 32 3889 32 632
Fragments arranged by size:
3889 632 89 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1537 2503 1537
Size: 966 3740
XbaI T'CTAG_A
Cuts at: 441 1737 441
Size: 1296 3410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1161 1504 2201 1161
Size: 343 697 3666
Fragments arranged by size:
3666 697 343
XmaI C'CCGG_G
Cuts at: 1726 1726
Size: 4706
XmnI GAAnn'nnTTC
Cuts at: 1024 1024
Size: 4706
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BcgI BfrBI BmtI BseRI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI