Restriction map for
pF1KB9948
(Circular) (Six-base) MAPSORT of: pF1KB9948.seq Check: 5092 from: 1 to: 5132
pF1KB9948 5132 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 517 517
Size: 5132
AatII G_ACGT'C
Cuts at: 623 623
Size: 5132
AccI GT'mk_AC
Cuts at: 2170 2170
Size: 5132
Acc65I G'GTAC_C
Cuts at: 2148 2148
Size: 5132
AclI AA'CG_TT
Cuts at: 4972 4972
Size: 5132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 1246 1892 2957 3389 4136 4404 889
Size: 357 646 1065 432 747 268 1617
Fragments arranged by size:
1617 1065 747 646 432 357 268
AfeI AGC'GCT
Cuts at: 532 532
Size: 5132
AflIII A'CryG_T
Cuts at: 428 2484 3588 4565 428
Size: 2056 1104 977 995
Fragments arranged by size:
2056 1104 995 977
AgeI A'CCGG_T
Cuts at: 3520 3520
Size: 5132
AleI CACnn'nnGTG
Cuts at: 1270 1270
Size: 5132
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1192 1192
Size: 5132
AlwNI CAG_nnn'CTG
Cuts at: 263 2437 4004 263
Size: 2174 1567 1391
Fragments arranged by size:
2174 1567 1391
ApaLI G'TGCA_C
Cuts at: 328 523 1263 3902 328
Size: 195 740 2639 1558
Fragments arranged by size:
2639 1558 740 195
ApoI r'AATT_y
Cuts at: 220 346 1018 1087 2071 2101 2136 4485 4741
Size: 126 672 69 984 30 35 2349 256
Cuts at: 4741 220
Size: 611
Fragments arranged by size:
2349 984 672 611 256 126 69 35
30
AseI AT'TA_AT
Cuts at: 20 20
Size: 5132
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
AvaI C'yCGr_G
Cuts at: 1636 2152 1636
Size: 516 4616
BamHI G'GATC_C
Cuts at: 2157 2157
Size: 5132
BanI G'GyrC_C
Cuts at: 178 338 1722 2148 2562 2810 2845 178
Size: 160 1384 426 414 248 35 2465
Fragments arranged by size:
2465 1384 426 414 248 160 35
BanII G_rGCy'C
Cuts at: 1208 1430 2146 3176 1208
Size: 222 716 1030 3164
Fragments arranged by size:
3164 1030 716 222
BbeI G_GCGC'C
Cuts at: 182 2566 2814 182
Size: 2384 248 2500
Fragments arranged by size:
2500 2384 248
BbsI GAAGACnn'nnnn_
Cuts at: 745 828 745
Size: 83 5049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1627 1661 1627
Size: 34 5098
BciVI GTATCCnnnnn_n'
Cuts at: 3030 3791 5132 3030
Size: 761 1341 3030
Fragments arranged by size:
3030 1341 761
BclI T'GATC_A
Cuts at: 2652 2652
Size: 5132
BfrBI ATG'CAT
Cuts at: 1900 1900
Size: 5132
BglI GCCn_nnn'nGGC
Cuts at: 560 560
Size: 5132
BglII A'GATC_T
Cuts at: 2647 4684 2647
Size: 2037 3095
BlpI GC'TnA_GC
Cuts at: 2244 2244
Size: 5132
Bme1580I G_kGCm'C
Cuts at: 332 527 1267 2757 2850 3906 332
Size: 195 740 1490 93 1056 1558
Fragments arranged by size:
1558 1490 1056 740 195 93
BmgBI CAC'GTC
Cuts at: 1257 1257
Size: 5132
BmrI ACTGGGnnnn_n'
Cuts at: 2344 2425 2760 4904 2344
Size: 81 335 2144 2572
Fragments arranged by size:
2572 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3499 3499
Size: 5132
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 1604 420
Size: 1184 3948
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2308 3187 3679 3977 2308
Size: 879 492 298 3463
Fragments arranged by size:
3463 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1103 4836 1103
Size: 3733 1399
BsaAI yAC'GTr
Cuts at: 431 693 1374 2485 431
Size: 262 681 1111 3078
Fragments arranged by size:
3078 1111 681 262
BsaBI GATnn'nnATC
Cuts at: 6 2646 4683 6
Size: 2640 2037 455
Fragments arranged by size:
2640 2037 455
BsaHI Gr'CG_yC
Cuts at: 179 620 2563 2811 3513 5009 179
Size: 441 1943 248 702 1496 302
Fragments arranged by size:
1943 1496 702 441 302 248
BsaWI w'CCGG_w
Cuts at: 1112 1907 2321 2842 3520 3794 3941 4352 1112
Size: 795 414 521 678 274 147 411 1892
Fragments arranged by size:
1892 795 678 521 414 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 418 448 1066 1096 418
Size: 30 618 30 4454
Fragments arranged by size:
4454 618 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 163
Size: 5132
BseYI C'CCAG_C
Cuts at: 550 1149 1319 1714 2557 3315 3892 4388 550
Size: 599 170 395 843 758 577 496 1294
Fragments arranged by size:
1294 843 758 599 577 496 395 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 249
Size: 5132
BsiEI CG_ry'CG
Cuts at: 104 588 1416 1998 2674 2720 3928 104
Size: 484 828 582 676 46 1208 1308
Fragments arranged by size:
1308 1208 828 676 582 484 46
BsiHKAI G_wGCw'C
Cuts at: 157 332 527 1208 1267 2146 2924 3114 3906
Size: 175 195 681 59 879 778 190 792
Cuts at: 3906 157
Size: 1383
Fragments arranged by size:
1383 879 792 778 681 195 190 175
59
Bsp1286I G_dGCh'C
Cuts at: 157 332 527 1208 1267 1430 2146 2757 2850
Size: 175 195 681 59 163 716 611 93
Cuts at: 2850 2924 3114 3176 3906 157
Size: 74 190 62 730 1383
Fragments arranged by size:
1383 730 716 681 611 195 190 175
163 93 74 62 59
BspEI T'CCGG_A
Cuts at: 4352 4352
Size: 5132
BspHI T'CATG_A
Cuts at: 4308 4308
Size: 5132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 2182 2396 2698 3079 517
Size: 1665 214 302 381 2570
Fragments arranged by size:
2570 1665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3424 3478 4962 5127 50
Size: 3374 54 1484 165 55
Fragments arranged by size:
3374 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3044 3044
Size: 5132
BsrFI r'CCGG_y
Cuts at: 1415 3130 3311 3520 1415
Size: 1715 181 209 3027
Fragments arranged by size:
3027 1715 209 181
BssSI C'ACGA_G
Cuts at: 3403 3761 3403
Size: 358 4774
BstAPI GCAn_nnn'nTGC
Cuts at: 263 1456 2509 263
Size: 1193 1053 2886
Fragments arranged by size:
2886 1193 1053
BstBI TT'CG_AA
Cuts at: 2075 3493 2075
Size: 1418 3714
BstYI r'GATC_y
Cuts at: 1 422 684 877 1220 1943 2157 2624 2647
Size: 421 262 193 343 723 214 467 23
Cuts at: 2647 2982 3228 4240 4326 4338 4684 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 723 467 449 421 346 343 335
262 246 214 193 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 184 184
Size: 5132
BtgI C'CryG_G
Cuts at: 107 582 1343 1670 107
Size: 475 761 327 3569
Fragments arranged by size:
3569 761 475 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1614 3082 3263 3426 1614
Size: 1468 181 163 3320
Fragments arranged by size:
3320 1468 181 163
BtsI GCAGTG_nn'
Cuts at: 261 466 2375 261
Size: 205 1909 3018
Fragments arranged by size:
3018 1909 205
DraI TTT'AAA
Cuts at: 2108 2131 2108
Size: 23 5109
DrdI GACnn_nn'nnGTC
Cuts at: 619 2044 2838 3696 619
Size: 1425 794 858 2055
Fragments arranged by size:
2055 1425 858 794
EaeI y'GGCC_r
Cuts at: 585 1413 1814 2717 2891 3282 3309 4654 585
Size: 828 401 903 174 391 27 1345 1063
Fragments arranged by size:
1345 1063 903 828 401 391 174 27
EagI C'GGCC_G
Cuts at: 585 1413 2717 585
Size: 828 1304 3000
Fragments arranged by size:
3000 1304 828
EarI CTCTTCn'nnn_
Cuts at: 1759 3155 3365 1759
Size: 1396 210 3526
Fragments arranged by size:
3526 1396 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3340 3650 3796 4942 3340
Size: 310 146 1146 3530
Fragments arranged by size:
3530 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1206 2144 1206
Size: 938 4194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 889 1246 1604 1892 2957 3389 4136 4404
Size: 469 357 358 288 1065 432 747 268
Cuts at: 4404 420
Size: 1148
Fragments arranged by size:
1148 1065 747 469 432 358 357 288
268
EcoNI CCTnn'n_nnAGG
Cuts at: 1228 1228
Size: 5132
EcoO109I rG'GnC_Cy
Cuts at: 1053 2271 1053
Size: 1218 3914
EcoRI G'AATT_C
Cuts at: 2071 2136 2071
Size: 65 5067
EcoRV GAT'ATC
Cuts at: 1887 1887
Size: 5132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1326 3151 1326
Size: 1825 3307
FspI TGC'GCA
Cuts at: 2913 2913
Size: 5132
HaeII r_GCGC'y
Cuts at: 182 374 534 582 2566 2814 3836 4815 182
Size: 192 160 48 1984 248 1022 979 499
Fragments arranged by size:
1984 1022 979 499 248 192 160 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 699 731 2974 3004 3006 3036 699
Size: 32 2243 30 2 30 2795
Fragments arranged by size:
2795 2243 32 30 30 2
HincII GTy'rAC
Cuts at: 2094 2171 2094
Size: 77 5055
HindIII A'AGCT_T
Cuts at: 1967 1967
Size: 5132
HpaI GTT'AAC
Cuts at: 2094 2094
Size: 5132
KasI G'GCGC_C
Cuts at: 178 2562 2810 178
Size: 2384 248 2500
Fragments arranged by size:
2500 2384 248
KpnI G_GTAC'C
Cuts at: 2152 2152
Size: 5132
MluI A'CGCG_T
Cuts at: 4565 4565
Size: 5132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3803 3987 3803
Size: 184 4948
MscI TGG'CCA
Cuts at: 1816 2893 1816
Size: 1077 4055
MslI CAynn'nnrTG
Cuts at: 511 1270 3121 3248 511
Size: 759 1851 127 2395
Fragments arranged by size:
2395 1851 759 127
MspA1I CmG'CkG
Cuts at: 584 798 2243 2557 2917 3930 4175 584
Size: 214 1445 314 360 1013 245 1541
Fragments arranged by size:
1541 1445 1013 360 314 245 214
NaeI GCC'GGC
Cuts at: 3313 3313
Size: 5132
NarI GG'CG_CC
Cuts at: 179 2563 2811 179
Size: 2384 248 2500
Fragments arranged by size:
2500 2384 248
NcoI C'CATG_G
Cuts at: 107 1343 107
Size: 1236 3896
NgoMIV G'CCGG_C
Cuts at: 3311 3311
Size: 5132
NotI GC'GGCC_GC
Cuts at: 585 585
Size: 5132
NsiI A_TGCA'T
Cuts at: 1902 1902
Size: 5132
NspI r_CATG'y
Cuts at: 1569 1900 2185 2685 3592 1569
Size: 331 285 500 907 3109
Fragments arranged by size:
3109 907 500 331 285
PciI A'CATG_T
Cuts at: 3588 3588
Size: 5132
PflMI CCAn_nnn'nTGG
Cuts at: 1344 1344
Size: 5132
PfoI T'CCnGG_A
Cuts at: 1055 1055
Size: 5132
PmeI GTTT'AAAC
Cuts at: 2131 2131
Size: 5132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 4271
Size: 32 5100
PpuMI rG'GwC_Cy
Cuts at: 1053 1053
Size: 5132
PshAI GACnn'nnGTC
Cuts at: 380 380
Size: 5132
PsiI TTA'TAA
Cuts at: 1958 1958
Size: 5132
PstI C_TGCA'G
Cuts at: 259 1452 2179 2864 259
Size: 1193 727 685 2527
Fragments arranged by size:
2527 1193 727 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5132
PvuII CAG'CTG
Cuts at: 798 2557 2917 798
Size: 1759 360 3013
Fragments arranged by size:
3013 1759 360
RsrII CG'GwC_CG
Cuts at: 3327 3327
Size: 5132
SacI G_AGCT'C
Cuts at: 1208 2146 1208
Size: 938 4194
SacII CC_GC'GG
Cuts at: 585 585
Size: 5132
SalI G'TCGA_C
Cuts at: 2169 2169
Size: 5132
SapI GCTCTTCn'nnn_
Cuts at: 3155 3365 3155
Size: 210 4922
SbfI CC_TGCA'GG
Cuts at: 2179 2179
Size: 5132
ScaI AGT'ACT
Cuts at: 961 4649 961
Size: 3688 1444
SfcI C'TryA_G
Cuts at: 33 255 1448 2175 2860 3853 4044 33
Size: 222 1193 727 685 993 191 1121
Fragments arranged by size:
1193 1121 993 727 685 222 191
SfoI GGC'GCC
Cuts at: 180 2564 2812 180
Size: 2384 248 2500
Fragments arranged by size:
2500 2384 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5132
SmaI CCC'GGG
Cuts at: 2154 2154
Size: 5132
SmlI C'TyrA_G
Cuts at: 2287 3202 3694 3956 2287
Size: 915 492 262 3463
Fragments arranged by size:
3463 915 492 262
SnaBI TAC'GTA
Cuts at: 693 693
Size: 5132
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5132
SphI G_CATG'C
Cuts at: 1900 2185 2685 1900
Size: 285 500 4347
Fragments arranged by size:
4347 500 285
SspI AAT'ATT
Cuts at: 2058 2058
Size: 5132
StyI C'CwwG_G
Cuts at: 107 1049 1343 1802 2266 2620 107
Size: 942 294 459 464 354 2619
Fragments arranged by size:
2619 942 464 459 354 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1141 1453 1859 2012 2715 2848 3307 3516 1141
Size: 312 406 153 703 133 459 209 2757
Fragments arranged by size:
2757 703 459 406 312 209 153 133
TatI w'GTAC_w
Cuts at: 959 1355 4647 959
Size: 396 3292 1444
Fragments arranged by size:
3292 1444 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 486 518 1998 2030 4649 4681 418
Size: 32 36 32 1480 32 2619 32 869
Fragments arranged by size:
2619 1480 869 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2929 2929
Size: 5132
XbaI T'CTAG_A
Cuts at: 2163 2163
Size: 5132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1809 2627 1809
Size: 818 4314
XmaI C'CCGG_G
Cuts at: 2152 2152
Size: 5132
XmnI GAAnn'nnTTC
Cuts at: 868 868
Size: 5132
ZraI GAC'GTC
Cuts at: 621 621
Size: 5132
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI ApaI AscI AvrII BaeI BbvCI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII FseI FspAI MfeI
NdeI NheI NruI PacI PasI PmlI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SrfI StuI SwaI
XhoI