Restriction map for
pF1KB9950
(Circular) (Six-base) MAPSORT of: pF1KB9950.seq Check: 932 from: 1 to: 5138
pF1KB9950 5138 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 461 461
Size: 5138
AccI GT'mk_AC
Cuts at: 2176 2176
Size: 5138
Acc65I G'GTAC_C
Cuts at: 2154 2154
Size: 5138
AclI AA'CG_TT
Cuts at: 4978 4978
Size: 5138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 983 1996 2963 3395 4142 4410 640
Size: 343 1013 967 432 747 268 1368
Fragments arranged by size:
1368 1013 967 747 432 343 268
AflIII A'CryG_T
Cuts at: 2490 3594 4571 2490
Size: 1104 977 3057
Fragments arranged by size:
3057 1104 977
AgeI A'CCGG_T
Cuts at: 3526 3526
Size: 5138
AhdI GACnn_n'nnGTC
Cuts at: 1314 1314
Size: 5138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 478 510 1812 1844 478
Size: 32 1302 32 3772
Fragments arranged by size:
3772 1302 32 32
AlwNI CAG_nnn'CTG
Cuts at: 640 2051 2443 4010 640
Size: 1411 392 1567 1768
Fragments arranged by size:
1768 1567 1411 392
ApaLI G'TGCA_C
Cuts at: 3908 3908
Size: 5138
ApoI r'AATT_y
Cuts at: 940 1074 1474 1528 2142 4491 4747 940
Size: 134 400 54 614 2349 256 1331
Fragments arranged by size:
2349 1331 614 400 256 134 54
AseI AT'TA_AT
Cuts at: 20 20
Size: 5138
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
AvaI C'yCGr_G
Cuts at: 310 629 2158 310
Size: 319 1529 3290
Fragments arranged by size:
3290 1529 319
BamHI G'GATC_C
Cuts at: 265 1904 2163 265
Size: 1639 259 3240
Fragments arranged by size:
3240 1639 259
BanI G'GyrC_C
Cuts at: 2154 2568 2816 2851 2154
Size: 414 248 35 4441
Fragments arranged by size:
4441 414 248 35
BanII G_rGCy'C
Cuts at: 1459 2152 3182 1459
Size: 693 1030 3415
Fragments arranged by size:
3415 1030 693
BbeI G_GCGC'C
Cuts at: 2572 2820 2572
Size: 248 4890
BbsI GAAGACnn'nnnn_
Cuts at: 827 2090 827
Size: 1263 3875
BciVI GTATCCnnnnn_n'
Cuts at: 105 3036 3797 5138 105
Size: 2931 761 1341 105
Fragments arranged by size:
2931 1341 761 105
BclI T'GATC_A
Cuts at: 1259 1445 2658 1259
Size: 186 1213 3739
Fragments arranged by size:
3739 1213 186
BfrBI ATG'CAT
Cuts at: 385 385
Size: 5138
BglII A'GATC_T
Cuts at: 1746 2653 4690 1746
Size: 907 2037 2194
Fragments arranged by size:
2194 2037 907
BlpI GC'TnA_GC
Cuts at: 2250 2250
Size: 5138
Bme1580I G_kGCm'C
Cuts at: 2763 2856 3912 2763
Size: 93 1056 3989
Fragments arranged by size:
3989 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2350 2431 2766 4910 2350
Size: 81 335 2144 2578
Fragments arranged by size:
2578 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3505 3505
Size: 5138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 1832 996
Size: 836 4302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2314 3193 3685 3983 2314
Size: 879 492 298 3469
Fragments arranged by size:
3469 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1986 4842 1986
Size: 2856 2282
BsaAI yAC'GTr
Cuts at: 1158 2491 1158
Size: 1333 3805
BsaBI GATnn'nnATC
Cuts at: 6 1020 2652 4689 6
Size: 1014 1632 2037 455
Fragments arranged by size:
2037 1632 1014 455
BsaHI Gr'CG_yC
Cuts at: 322 2569 2817 3519 5015 322
Size: 2247 248 702 1496 445
Fragments arranged by size:
2247 1496 702 445 248
BsaWI w'CCGG_w
Cuts at: 2327 2848 3526 3800 3947 4358 2327
Size: 521 678 274 147 411 3107
Fragments arranged by size:
3107 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 730 700
Size: 30 5108
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 328 1626 328
Size: 1298 3840
BseYI C'CCAG_C
Cuts at: 719 785 1374 1404 1640 2563 3321 3898 4394
Size: 66 589 30 236 923 758 577 496
Cuts at: 4394 719
Size: 1463
Fragments arranged by size:
1463 923 758 589 577 496 236 66
30
BsiEI CG_ry'CG
Cuts at: 104 2680 2726 3934 104
Size: 2576 46 1208 1308
Fragments arranged by size:
2576 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2152 2930 3120 3912 2152
Size: 778 190 792 3378
Fragments arranged by size:
3378 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1459 2152 2763 2856 2930 3120 3182 3912 1459
Size: 693 611 93 74 190 62 730 2685
Fragments arranged by size:
2685 730 693 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4358 4358
Size: 5138
BspHI T'CATG_A
Cuts at: 4314 4314
Size: 5138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 461 2188 2402 2704 3085 461
Size: 1727 214 302 381 2514
Fragments arranged by size:
2514 1727 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3430 3484 4968 5133 50
Size: 3380 54 1484 165 55
Fragments arranged by size:
3380 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1593 3050 1593
Size: 1457 3681
BsrFI r'CCGG_y
Cuts at: 2003 3136 3317 3526 2003
Size: 1133 181 209 3615
Fragments arranged by size:
3615 1133 209 181
BsrGI T'GTAC_A
Cuts at: 1094 2042 1094
Size: 948 4190
BssSI C'ACGA_G
Cuts at: 2056 3409 3767 2056
Size: 1353 358 3427
Fragments arranged by size:
3427 1353 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1592 2515 1592
Size: 923 4215
BstBI TT'CG_AA
Cuts at: 3499 3499
Size: 5138
BstEII G'GTnAC_C
Cuts at: 442 442
Size: 5138
BstXI CCAn_nnnn'nTGG
Cuts at: 1838 1838
Size: 5138
BstYI r'GATC_y
Cuts at: 1 265 1746 1904 2163 2630 2653 2988 3234
Size: 264 1481 158 259 467 23 335 246
Cuts at: 3234 4246 4332 4344 4690 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1481 1012 467 449 346 335 264 259
246 158 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1609 1609
Size: 5138
BtgI C'CryG_G
Cuts at: 107 107
Size: 5138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3088 3269 3432 3088
Size: 181 163 4794
Fragments arranged by size:
4794 181 163
BtsI GCAGTG_nn'
Cuts at: 876 2381 876
Size: 1505 3633
DraI TTT'AAA
Cuts at: 2137 2137
Size: 5138
DraIII CAC_nnn'GTG
Cuts at: 1281 1281
Size: 5138
DrdI GACnn_nn'nnGTC
Cuts at: 1804 2844 3702 1804
Size: 1040 858 3240
Fragments arranged by size:
3240 1040 858
EaeI y'GGCC_r
Cuts at: 1383 2723 2897 3288 3315 4660 1383
Size: 1340 174 391 27 1345 1861
Fragments arranged by size:
1861 1345 1340 391 174 27
EagI C'GGCC_G
Cuts at: 2723 2723
Size: 5138
EarI CTCTTCn'nnn_
Cuts at: 389 575 962 1346 3161 3371 389
Size: 186 387 384 1815 210 2156
Fragments arranged by size:
2156 1815 387 384 210 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1261 3346 3656 3802 4948 1261
Size: 2085 310 146 1146 1451
Fragments arranged by size:
2085 1451 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2150 2150
Size: 5138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 983 996 1832 1996 2963 3395 4142 4410
Size: 343 13 836 164 967 432 747 268
Cuts at: 4410 640
Size: 1368
Fragments arranged by size:
1368 967 836 747 432 343 268 164
13
EcoO109I rG'GnC_Cy
Cuts at: 1302 2277 1302
Size: 975 4163
EcoRI G'AATT_C
Cuts at: 940 1474 2142 940
Size: 534 668 3936
Fragments arranged by size:
3936 668 534
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 154 211 385 3157 154
Size: 57 174 2772 2135
Fragments arranged by size:
2772 2135 174 57
FspI TGC'GCA
Cuts at: 2919 2919
Size: 5138
HaeII r_GCGC'y
Cuts at: 2572 2820 3842 4821 2572
Size: 248 1022 979 2889
Fragments arranged by size:
2889 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 390 422 524 556 646 678 682
Size: 32 255 32 102 32 90 32 4
Cuts at: 682 714 748 780 1300 1332 2980 3010 3012
Size: 32 34 32 520 32 1648 30 2
Cuts at: 3012 3042 103
Size: 30 2199
Fragments arranged by size:
2199 1648 520 255 102 90 34 32
32 32 32 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 1286 2177 1286
Size: 891 4247
HindIII A'AGCT_T
Cuts at: 1222 1222
Size: 5138
KasI G'GCGC_C
Cuts at: 2568 2816 2568
Size: 248 4890
KpnI G_GTAC'C
Cuts at: 2158 2158
Size: 5138
MluI A'CGCG_T
Cuts at: 4571 4571
Size: 5138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1797 3809 3993 1797
Size: 2012 184 2942
Fragments arranged by size:
2942 2012 184
MscI TGG'CCA
Cuts at: 1385 2899 1385
Size: 1514 3624
MslI CAynn'nnrTG
Cuts at: 1166 3127 3254 1166
Size: 1961 127 3050
Fragments arranged by size:
3050 1961 127
MspA1I CmG'CkG
Cuts at: 1404 2048 2249 2563 2923 3936 4181 1404
Size: 644 201 314 360 1013 245 2361
Fragments arranged by size:
2361 1013 644 360 314 245 201
NaeI GCC'GGC
Cuts at: 3319 3319
Size: 5138
NarI GG'CG_CC
Cuts at: 2569 2817 2569
Size: 248 4890
NcoI C'CATG_G
Cuts at: 107 107
Size: 5138
NdeI CA'TA_TG
Cuts at: 552 552
Size: 5138
NgoMIV G'CCGG_C
Cuts at: 3317 3317
Size: 5138
NsiI A_TGCA'T
Cuts at: 387 387
Size: 5138
NspI r_CATG'y
Cuts at: 804 2134 2191 2691 3598 804
Size: 1330 57 500 907 2344
Fragments arranged by size:
2344 1330 907 500 57
PasI CC'CwG_GG
Cuts at: 1320 1986 1320
Size: 666 4472
PciI A'CATG_T
Cuts at: 3594 3594
Size: 5138
PflMI CCAn_nnn'nTGG
Cuts at: 456 456
Size: 5138
PmeI GTTT'AAAC
Cuts at: 2137 2137
Size: 5138
PmlI CAC'GTG
Cuts at: 1158 1158
Size: 5138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 125 157 1019 1051 4277 4309 125
Size: 32 862 32 3226 32 954
Fragments arranged by size:
3226 954 862 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1302 1302
Size: 5138
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1168 1200 1168
Size: 32 5106
PstI C_TGCA'G
Cuts at: 573 2185 2870 573
Size: 1612 685 2841
Fragments arranged by size:
2841 1612 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5138
PvuII CAG'CTG
Cuts at: 1404 2048 2563 2923 1404
Size: 644 515 360 3619
Fragments arranged by size:
3619 644 515 360
RsrII CG'GwC_CG
Cuts at: 3333 3333
Size: 5138
SacI G_AGCT'C
Cuts at: 2152 2152
Size: 5138
SalI G'TCGA_C
Cuts at: 2175 2175
Size: 5138
SapI GCTCTTCn'nnn_
Cuts at: 3161 3371 3161
Size: 210 4928
SbfI CC_TGCA'GG
Cuts at: 2185 2185
Size: 5138
ScaI AGT'ACT
Cuts at: 4655 4655
Size: 5138
SfcI C'TryA_G
Cuts at: 33 326 569 2181 2866 3859 4050 33
Size: 293 243 1612 685 993 191 1121
Fragments arranged by size:
1612 1121 993 685 293 243 191
SfoI GGC'GCC
Cuts at: 2570 2818 2570
Size: 248 4890
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
SmaI CCC'GGG
Cuts at: 2160 2160
Size: 5138
SmlI C'TyrA_G
Cuts at: 310 2293 3208 3700 3962 310
Size: 1983 915 492 262 1486
Fragments arranged by size:
1983 1486 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5138
SphI G_CATG'C
Cuts at: 2191 2691 2191
Size: 500 4638
StyI C'CwwG_G
Cuts at: 107 814 2272 2626 107
Size: 707 1458 354 2619
Fragments arranged by size:
2619 1458 707 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2721 2854 3313 3522 2721
Size: 133 459 209 4337
Fragments arranged by size:
4337 459 209 133
TatI w'GTAC_w
Cuts at: 1094 2042 4653 1094
Size: 948 2611 1579
Fragments arranged by size:
2611 1579 948
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4655 4687 4655
Size: 32 5106
Tth111I GACn'n_nGTC
Cuts at: 2935 2935
Size: 5138
XbaI T'CTAG_A
Cuts at: 126 2169 126
Size: 2043 3095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2633 2633
Size: 5138
XhoI C'TCGA_G
Cuts at: 310 310
Size: 5138
XmaI C'CCGG_G
Cuts at: 2158 2158
Size: 5138
XmnI GAAnn'nnTTC
Cuts at: 134 137 1478 2111 134
Size: 3 1341 633 3161
Fragments arranged by size:
3161 1341 633 3
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI ApaI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BssHII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI PacI
PfoI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI