Restriction map for
pF1KB9951
(Circular) (Six-base) MAPSORT of: pF1KB9951.seq Check: 6869 from: 1 to: 4718
pF1KB9951 4718 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 1756 1756
Size: 4718
Acc65I G'GTAC_C
Cuts at: 1734 1734
Size: 4718
AclI AA'CG_TT
Cuts at: 4558 4558
Size: 4718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 601 613 1525 2543 2975 3722 3990 158
Size: 443 12 912 1018 432 747 268 886
Fragments arranged by size:
1018 912 886 747 443 432 268 12
AflIII A'CryG_T
Cuts at: 563 2070 3174 4151 563
Size: 1507 1104 977 1130
Fragments arranged by size:
1507 1130 1104 977
AgeI A'CCGG_T
Cuts at: 3106 3106
Size: 4718
AleI CACnn'nnGTG
Cuts at: 686 862 686
Size: 176 4542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1456 1456
Size: 4718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1058 1090 1058
Size: 32 4686
AlwNI CAG_nnn'CTG
Cuts at: 181 1696 2023 3590 181
Size: 1515 327 1567 1309
Fragments arranged by size:
1567 1515 1309 327
ApaLI G'TGCA_C
Cuts at: 648 902 3488 648
Size: 254 2586 1878
Fragments arranged by size:
2586 1878 254
ApoI r'AATT_y
Cuts at: 1282 1722 4071 4327 1282
Size: 440 2349 256 1673
Fragments arranged by size:
2349 1673 440 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
AvaI C'yCGr_G
Cuts at: 1567 1738 1567
Size: 171 4547
BamHI G'GATC_C
Cuts at: 1743 1743
Size: 4718
BanI G'GyrC_C
Cuts at: 1529 1734 2148 2396 2431 1529
Size: 205 414 248 35 3816
Fragments arranged by size:
3816 414 248 205 35
BanII G_rGCy'C
Cuts at: 1732 2762 1732
Size: 1030 3688
BbeI G_GCGC'C
Cuts at: 2152 2400 2152
Size: 248 4470
BbsI GAAGACnn'nnnn_
Cuts at: 604 805 1001 604
Size: 201 196 4321
Fragments arranged by size:
4321 201 196
BbvCI CC'TCA_GC
Cuts at: 222 1418 222
Size: 1196 3522
BciVI GTATCCnnnnn_n'
Cuts at: 360 1182 2616 3377 4718 360
Size: 822 1434 761 1341 360
Fragments arranged by size:
1434 1341 822 761 360
BclI T'GATC_A
Cuts at: 2238 2238
Size: 4718
BglI GCCn_nnn'nGGC
Cuts at: 291 1204 291
Size: 913 3805
BglII A'GATC_T
Cuts at: 2233 4270 2233
Size: 2037 2681
BlpI GC'TnA_GC
Cuts at: 1580 1830 1580
Size: 250 4468
Bme1580I G_kGCm'C
Cuts at: 652 906 1100 1385 2343 2436 3492 652
Size: 254 194 285 958 93 1056 1878
Fragments arranged by size:
1878 1056 958 285 254 194 93
BmrI ACTGGGnnnn_n'
Cuts at: 1930 2011 2346 4490 1930
Size: 81 335 2144 2158
Fragments arranged by size:
2158 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 4718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 943 928
Size: 15 4703
Bpu10I CC'TnA_GC
Cuts at: 222 670 1418 1534 222
Size: 448 748 116 3406
Fragments arranged by size:
3406 748 448 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1894 2773 3265 3563 1894
Size: 879 492 298 3049
Fragments arranged by size:
3049 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1639 4422 1639
Size: 2783 1935
BsaAI yAC'GTr
Cuts at: 2071 2071
Size: 4718
BsaBI GATnn'nnATC
Cuts at: 6 2232 4269 6
Size: 2226 2037 455
Fragments arranged by size:
2226 2037 455
BsaHI Gr'CG_yC
Cuts at: 2149 2397 3099 4595 2149
Size: 248 702 1496 2272
Fragments arranged by size:
2272 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1907 2428 3106 3380 3527 3938 1907
Size: 521 678 274 147 411 2687
Fragments arranged by size:
2687 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 106 136 667 697 871 901 106
Size: 30 531 30 174 30 3923
Fragments arranged by size:
3923 531 174 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1183 1375 1486 1698 1183
Size: 192 111 212 4203
Fragments arranged by size:
4203 212 192 111
BseYI C'CCAG_C
Cuts at: 1080 1343 1593 2143 2901 3478 3974 1080
Size: 263 250 550 758 577 496 1824
Fragments arranged by size:
1824 758 577 550 496 263 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1609 664
Size: 945 3773
BsiEI CG_ry'CG
Cuts at: 104 2260 2306 3514 104
Size: 2156 46 1208 1308
Fragments arranged by size:
2156 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 652 906 1732 2510 2700 3492 652
Size: 254 826 778 190 792 1878
Fragments arranged by size:
1878 826 792 778 254 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1710 1710
Size: 4718
Bsp1286I G_dGCh'C
Cuts at: 652 906 1100 1385 1732 2343 2436 2510 2700
Size: 254 194 285 347 611 93 74 190
Cuts at: 2700 2762 3492 652
Size: 62 730 1878
Fragments arranged by size:
1878 730 611 347 285 254 194 190
93 74 62
BspEI T'CCGG_A
Cuts at: 3938 3938
Size: 4718
BspHI T'CATG_A
Cuts at: 3894 3894
Size: 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 686 1768 1982 2284 2665 686
Size: 1082 214 302 381 2739
Fragments arranged by size:
2739 1082 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3010 3064 4548 4713 50
Size: 2960 54 1484 165 55
Fragments arranged by size:
2960 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 942 2630 942
Size: 1688 3030
BsrFI r'CCGG_y
Cuts at: 1526 1622 2716 2897 3106 1526
Size: 96 1094 181 209 3138
Fragments arranged by size:
3138 1094 209 181 96
BssSI C'ACGA_G
Cuts at: 2989 3347 2989
Size: 358 4360
BstAPI GCAn_nnn'nTGC
Cuts at: 2095 2095
Size: 4718
BstBI TT'CG_AA
Cuts at: 3079 3079
Size: 4718
BstXI CCAn_nnnn'nTGG
Cuts at: 1185 1185
Size: 4718
BstYI r'GATC_y
Cuts at: 1 1307 1743 2210 2233 2568 2814 3826 3912
Size: 1306 436 467 23 335 246 1012 86
Cuts at: 3912 3924 4270 1
Size: 12 346 449
Fragments arranged by size:
1306 1012 467 449 436 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 362 705 362
Size: 343 4375
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2668 2849 3012 2668
Size: 181 163 4374
Fragments arranged by size:
4374 181 163
BtsI GCAGTG_nn'
Cuts at: 147 881 923 981 1122 1456 1961 147
Size: 734 42 58 141 334 505 2904
Fragments arranged by size:
2904 734 505 334 141 58 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 857 892 857
Size: 35 4683
DraI TTT'AAA
Cuts at: 1717 1717
Size: 4718
DrdI GACnn_nn'nnGTC
Cuts at: 195 2424 3282 195
Size: 2229 858 1631
Fragments arranged by size:
2229 1631 858
EaeI y'GGCC_r
Cuts at: 359 2303 2477 2868 2895 4240 359
Size: 1944 174 391 27 1345 837
Fragments arranged by size:
1944 1345 837 391 174 27
EagI C'GGCC_G
Cuts at: 2303 2303
Size: 4718
EarI CTCTTCn'nnn_
Cuts at: 272 298 308 806 1224 1637 2741 2951 272
Size: 26 10 498 418 413 1104 210 2039
Fragments arranged by size:
2039 1104 498 418 413 210 26 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2926 3236 3382 4528 2926
Size: 310 146 1146 3116
Fragments arranged by size:
3116 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1730 1730
Size: 4718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 601 613 928 943 1525 2543 2975 3722
Size: 443 12 315 15 582 1018 432 747
Cuts at: 3722 3990 158
Size: 268 886
Fragments arranged by size:
1018 886 747 582 443 432 315 268
15 12
EcoO109I rG'GnC_Cy
Cuts at: 1857 1857
Size: 4718
EcoRI G'AATT_C
Cuts at: 1722 1722
Size: 4718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4718
FspI TGC'GCA
Cuts at: 2499 2499
Size: 4718
HaeII r_GCGC'y
Cuts at: 297 2152 2400 3422 4401 297
Size: 1855 248 1022 979 614
Fragments arranged by size:
1855 1022 979 614 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 871 903 1299 1331 1354 1386 2560
Size: 32 733 32 396 32 23 32 1174
Cuts at: 2560 2590 2592 2622 106
Size: 30 2 30 2202
Fragments arranged by size:
2202 1174 733 396 32 32 32 32
30 30 23 2
HincII GTy'rAC
Cuts at: 1628 1757 1628
Size: 129 4589
KasI G'GCGC_C
Cuts at: 2148 2396 2148
Size: 248 4470
KpnI G_GTAC'C
Cuts at: 1738 1738
Size: 4718
MfeI C'AATT_G
Cuts at: 475 475
Size: 4718
MluI A'CGCG_T
Cuts at: 4151 4151
Size: 4718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 1426 1490 3389 3573 92
Size: 1334 64 1899 184 1237
Fragments arranged by size:
1899 1334 1237 184 64
MscI TGG'CCA
Cuts at: 361 2479 361
Size: 2118 2600
MslI CAynn'nnrTG
Cuts at: 686 862 2707 2834 686
Size: 176 1845 127 2570
Fragments arranged by size:
2570 1845 176 127
MspA1I CmG'CkG
Cuts at: 226 1417 1593 1696 1829 2143 2503 3516 3761
Size: 1191 176 103 133 314 360 1013 245
Cuts at: 3761 226
Size: 1183
Fragments arranged by size:
1191 1183 1013 360 314 245 176 133
103
NaeI GCC'GGC
Cuts at: 2899 2899
Size: 4718
NarI GG'CG_CC
Cuts at: 2149 2397 2149
Size: 248 4470
NcoI C'CATG_G
Cuts at: 362 705 362
Size: 343 4375
NdeI CA'TA_TG
Cuts at: 951 951
Size: 4718
NgoMIV G'CCGG_C
Cuts at: 2897 2897
Size: 4718
NspI r_CATG'y
Cuts at: 567 900 1771 2271 3178 567
Size: 333 871 500 907 2107
Fragments arranged by size:
2107 907 871 500 333
PciI A'CATG_T
Cuts at: 563 3174 563
Size: 2611 2107
PflMI CCAn_nnn'nTGG
Cuts at: 847 847
Size: 4718
PmeI GTTT'AAAC
Cuts at: 1717 1717
Size: 4718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 391 423 1223 1255 3857 3889 391
Size: 32 800 32 2602 32 1220
Fragments arranged by size:
2602 1220 800 32 32 32
PstI C_TGCA'G
Cuts at: 437 1034 1221 1765 2450 437
Size: 597 187 544 685 2705
Fragments arranged by size:
2705 685 597 544 187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4718
PvuII CAG'CTG
Cuts at: 226 1417 1593 2143 2503 226
Size: 1191 176 550 360 2441
Fragments arranged by size:
2441 1191 550 360 176
RsrII CG'GwC_CG
Cuts at: 2913 2913
Size: 4718
SacI G_AGCT'C
Cuts at: 1732 1732
Size: 4718
SalI G'TCGA_C
Cuts at: 1755 1755
Size: 4718
SapI GCTCTTCn'nnn_
Cuts at: 806 2741 2951 806
Size: 1935 210 2573
Fragments arranged by size:
2573 1935 210
SbfI CC_TGCA'GG
Cuts at: 1221 1765 1221
Size: 544 4174
ScaI AGT'ACT
Cuts at: 134 4235 134
Size: 4101 617
SexAI A'CCwGG_T
Cuts at: 336 336
Size: 4718
SfcI C'TryA_G
Cuts at: 33 433 1030 1217 1761 2446 3439 3630 33
Size: 400 597 187 544 685 993 191 1121
Fragments arranged by size:
1121 993 685 597 544 400 191 187
SfoI GGC'GCC
Cuts at: 2150 2398 2150
Size: 248 4470
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
SmaI CCC'GGG
Cuts at: 1740 1740
Size: 4718
SmlI C'TyrA_G
Cuts at: 1873 2788 3280 3542 1873
Size: 915 492 262 3049
Fragments arranged by size:
3049 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1192 67
Size: 1125 3593
SphI G_CATG'C
Cuts at: 1771 2271 1771
Size: 500 4218
SspI AAT'ATT
Cuts at: 511 511
Size: 4718
StyI C'CwwG_G
Cuts at: 362 705 1852 2206 362
Size: 343 1147 354 2874
Fragments arranged by size:
2874 1147 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1094 2301 2434 2893 3102 1094
Size: 1207 133 459 209 2710
Fragments arranged by size:
2710 1207 459 209 133
TatI w'GTAC_w
Cuts at: 132 1250 4233 132
Size: 1118 2983 617
Fragments arranged by size:
2983 1118 617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4235 4267 4235
Size: 32 4686
Tth111I GACn'n_nGTC
Cuts at: 2515 2515
Size: 4718
XbaI T'CTAG_A
Cuts at: 990 1164 1749 990
Size: 174 585 3959
Fragments arranged by size:
3959 585 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2213 2213
Size: 4718
XmaI C'CCGG_G
Cuts at: 1738 1738
Size: 4718
XmnI GAAnn'nnTTC
Cuts at: 446 1238 1657 446
Size: 792 419 3507
Fragments arranged by size:
3507 792 419
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI