Restriction map for
pF1KB9953
(Circular) (Six-base) MAPSORT of: pF1KB9953.seq Check: 9884 from: 1 to: 4721
pF1KB9953 4721 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 237 1248 1290 237
Size: 1011 42 3668
Fragments arranged by size:
3668 1011 42
AccI GT'mk_AC
Cuts at: 1759 1759
Size: 4721
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 823 2546 2978 3725 3993 823
Size: 1723 432 747 268 1551
Fragments arranged by size:
1723 1551 747 432 268
AfeI AGC'GCT
Cuts at: 637 637
Size: 4721
AflIII A'CryG_T
Cuts at: 431 2073 3177 4154 431
Size: 1642 1104 977 998
Fragments arranged by size:
1642 1104 998 977
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AleI CACnn'nnGTG
Cuts at: 683 683
Size: 4721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1276 1276
Size: 4721
AlwNI CAG_nnn'CTG
Cuts at: 462 504 843 897 954 978 1095 1326 1359
Size: 42 339 54 57 24 117 231 33
Cuts at: 1359 1437 2026 3593 462
Size: 78 589 1567 1590
Fragments arranged by size:
1590 1567 589 339 231 117 78 57
54 42 33 24
ApaLI G'TGCA_C
Cuts at: 3491 3491
Size: 4721
ApoI r'AATT_y
Cuts at: 1725 4074 4330 1725
Size: 2349 256 2116
Fragments arranged by size:
2349 2116 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 346 884 1223 1570 1741 346
Size: 538 339 347 171 3326
Fragments arranged by size:
3326 538 347 339 171
BamHI G'GATC_C
Cuts at: 1746 1746
Size: 4721
BanI G'GyrC_C
Cuts at: 697 1586 1737 2151 2399 2434 697
Size: 889 151 414 248 35 2984
Fragments arranged by size:
2984 889 414 248 151 35
BanII G_rGCy'C
Cuts at: 362 523 632 1381 1421 1489 1714 1735 2765
Size: 161 109 749 40 68 225 21 1030
Cuts at: 2765 362
Size: 2318
Fragments arranged by size:
2318 1030 749 225 161 109 68 40
21
BbeI G_GCGC'C
Cuts at: 701 1590 2155 2403 701
Size: 889 565 248 3019
Fragments arranged by size:
3019 889 565 248
BbsI GAAGACnn'nnnn_
Cuts at: 405 405
Size: 4721
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3380 4721 2619
Size: 761 1341 2619
Fragments arranged by size:
2619 1341 761
BclI T'GATC_A
Cuts at: 422 2241 422
Size: 1819 2902
BglI GCCn_nnn'nGGC
Cuts at: 329 1475 329
Size: 1146 3575
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 253 517 1833 253
Size: 264 1316 3141
Fragments arranged by size:
3141 1316 264
Bme1580I G_kGCm'C
Cuts at: 2346 2439 3495 2346
Size: 93 1056 3572
Fragments arranged by size:
3572 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1933 2014 2349 4493 1933
Size: 81 335 2144 2161
Fragments arranged by size:
2161 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 376 751 862 919 976 1381 1459 169
Size: 207 375 111 57 57 405 78 3431
Fragments arranged by size:
3431 405 375 207 111 78 57 57
Bpu10I CC'TnA_GC
Cuts at: 808 1345 1375 1552 808
Size: 537 30 177 3977
Fragments arranged by size:
3977 537 177 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 616 730 1897 2776 3268 3566 583
Size: 33 114 1167 879 492 298 1738
Fragments arranged by size:
1738 1167 879 492 298 114 33
BsaI GGTCTCn'nnnn_
Cuts at: 4425 4425
Size: 4721
BsaAI yAC'GTr
Cuts at: 336 1105 2074 336
Size: 769 969 2983
Fragments arranged by size:
2983 969 769
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 698 1050 1587 2152 2400 3102 4598 698
Size: 352 537 565 248 702 1496 821
Fragments arranged by size:
1496 821 702 565 537 352 248
BsaWI w'CCGG_w
Cuts at: 1055 1910 2431 3109 3383 3530 3941 1055
Size: 855 521 678 274 147 411 1835
Fragments arranged by size:
1835 855 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 832 862 832
Size: 30 4691
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 619 661 703 973 1081 1174 1216 1678
Size: 363 42 42 270 108 93 42 462
Cuts at: 1678 256
Size: 3299
Fragments arranged by size:
3299 462 363 270 108 93 42 42
42
BseYI C'CCAG_C
Cuts at: 832 1269 1448 2146 2904 3481 3977 832
Size: 437 179 698 758 577 496 1576
Fragments arranged by size:
1576 758 698 577 496 437 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 610 910 1192 1495 517
Size: 93 300 282 303 3743
Fragments arranged by size:
3743 303 300 282 93
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3517 104
Size: 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 254 299 362 523 632 1381 1421 1735 2513
Size: 45 63 161 109 749 40 314 778
Cuts at: 2513 2703 3495 254
Size: 190 792 1480
Fragments arranged by size:
1480 792 778 749 314 190 161 109
63 45 40
BsmBI CGTCTCn'nnnn_
Cuts at: 1357 1357
Size: 4721
Bsp1286I G_dGCh'C
Cuts at: 254 299 362 523 632 1381 1421 1489 1714
Size: 45 63 161 109 749 40 68 225
Cuts at: 1714 1735 2346 2439 2513 2703 2765 3495 254
Size: 21 611 93 74 190 62 730 1480
Fragments arranged by size:
1480 749 730 611 225 190 161 109
93 74 68 63 62 45 40 21
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 476 758 3897 476
Size: 282 3139 1300
Fragments arranged by size:
3139 1300 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 237 731 1248 1290 1512 1771 1985 2287
Size: 22 494 517 42 222 259 214 302
Cuts at: 2287 2668 215
Size: 381 2268
Fragments arranged by size:
2268 517 494 381 302 259 222 214
42 22
BsrBI CCG'CTC
Cuts at: 50 1176 1581 3013 3067 4551 4716 50
Size: 1126 405 1432 54 1484 165 55
Fragments arranged by size:
1484 1432 1126 405 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2633 2633
Size: 4721
BsrFI r'CCGG_y
Cuts at: 319 402 616 1474 2719 2900 3109 319
Size: 83 214 858 1245 181 209 1931
Fragments arranged by size:
1931 1245 858 214 209 181 83
BsrGI T'GTAC_A
Cuts at: 377 1121 377
Size: 744 3977
BssHII G'CGCG_C
Cuts at: 1623 1623
Size: 4721
BssSI C'ACGA_G
Cuts at: 2992 3350 2992
Size: 358 4363
BstAPI GCAn_nnn'nTGC
Cuts at: 978 1527 2098 978
Size: 549 571 3601
Fragments arranged by size:
3601 571 549
BstBI TT'CG_AA
Cuts at: 3082 3082
Size: 4721
BstXI CCAn_nnnn'nTGG
Cuts at: 672 672
Size: 4721
BstYI r'GATC_y
Cuts at: 1 1746 2213 2236 2571 2817 3829 3915 3927
Size: 1745 467 23 335 246 1012 86 12
Cuts at: 3927 4273 1
Size: 346 449
Fragments arranged by size:
1745 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1630 1630
Size: 4721
BtgI C'CryG_G
Cuts at: 818 1331 1682 818
Size: 513 351 3857
Fragments arranged by size:
3857 513 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 220 1096 1964 220
Size: 876 868 2977
Fragments arranged by size:
2977 876 868
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3285 2427
Size: 858 3863
EaeI y'GGCC_r
Cuts at: 321 662 1328 2306 2480 2871 2898 4243 321
Size: 341 666 978 174 391 27 1345 799
Fragments arranged by size:
1345 978 799 666 391 341 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1021 1297 2929 3239 3385 4531 1021
Size: 276 1632 310 146 1146 1211
Fragments arranged by size:
1632 1211 1146 310 276 146
EcoICRI GAG'CTC
Cuts at: 360 521 630 1379 1419 1733 360
Size: 161 109 749 40 314 3348
Fragments arranged by size:
3348 749 314 161 109 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 376 751 823 862 919 976 1381 1459
Size: 207 375 72 39 57 57 405 78
Cuts at: 1459 2546 2978 3725 3993 169
Size: 1087 432 747 268 897
Fragments arranged by size:
1087 897 747 432 405 375 268 207
78 72 57 57 39
EcoNI CCTnn'n_nnAGG
Cuts at: 760 877 1021 1033 760
Size: 117 144 12 4448
Fragments arranged by size:
4448 144 117 12
EcoO109I rG'GnC_Cy
Cuts at: 222 501 870 1026 1227 1409 1434 1860 222
Size: 279 369 156 201 182 25 426 3083
Fragments arranged by size:
3083 426 369 279 201 182 156 25
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1486 2740 1486
Size: 1254 3467
FspI TGC'GCA
Cuts at: 571 1021 1252 2502 571
Size: 450 231 1250 2790
Fragments arranged by size:
2790 1250 450 231
HaeII r_GCGC'y
Cuts at: 639 701 979 1590 2155 2403 3425 4404 639
Size: 62 278 611 565 248 1022 979 956
Fragments arranged by size:
1022 979 956 611 565 278 248 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2563 2593 2595 2625 2563
Size: 30 2 30 4659
Fragments arranged by size:
4659 30 30 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
KasI G'GCGC_C
Cuts at: 697 1586 2151 2399 697
Size: 889 565 248 3019
Fragments arranged by size:
3019 889 565 248
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MluI A'CGCG_T
Cuts at: 431 4154 431
Size: 3723 998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 1406 3392 3576 92
Size: 1314 1986 184 1237
Fragments arranged by size:
1986 1314 1237 184
MscI TGG'CCA
Cuts at: 664 2482 664
Size: 1818 2903
MslI CAynn'nnrTG
Cuts at: 683 2710 2837 683
Size: 2027 127 2567
Fragments arranged by size:
2567 2027 127
MspA1I CmG'CkG
Cuts at: 315 375 459 729 828 1296 1359 1559 1832
Size: 60 84 270 99 468 63 200 273
Cuts at: 1832 2146 2506 3519 3764 315
Size: 314 360 1013 245 1272
Fragments arranged by size:
1272 1013 468 360 314 273 270 245
200 99 84 63 60
NaeI GCC'GGC
Cuts at: 404 618 1476 2902 404
Size: 214 858 1426 2223
Fragments arranged by size:
2223 1426 858 214
NarI GG'CG_CC
Cuts at: 698 1587 2152 2400 698
Size: 889 565 248 3019
Fragments arranged by size:
3019 889 565 248
NcoI C'CATG_G
Cuts at: 818 818
Size: 4721
NgoMIV G'CCGG_C
Cuts at: 402 616 1474 2900 402
Size: 214 858 1426 2223
Fragments arranged by size:
2223 1426 858 214
NspI r_CATG'y
Cuts at: 1774 2274 3181 1774
Size: 500 907 3314
Fragments arranged by size:
3314 907 500
PciI A'CATG_T
Cuts at: 3177 3177
Size: 4721
PflMI CCAn_nnn'nTGG
Cuts at: 954 1437 954
Size: 483 4238
PfoI T'CCnGG_A
Cuts at: 146 299 911 146
Size: 153 612 3956
Fragments arranged by size:
3956 612 153
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PpuMI rG'GwC_Cy
Cuts at: 222 501 1026 1409 1434 222
Size: 279 525 383 25 3509
Fragments arranged by size:
3509 525 383 279 25
PshAI GACnn'nnGTC
Cuts at: 472 1369 472
Size: 897 3824
PstI C_TGCA'G
Cuts at: 314 488 728 860 1091 1154 1235 1295 1301
Size: 174 240 132 231 63 81 60 6
Cuts at: 1301 1768 2453 314
Size: 467 685 2582
Fragments arranged by size:
2582 685 467 240 231 174 132 81
63 60 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 375 459 729 1296 1359 2146 2506 375
Size: 84 270 567 63 787 360 2590
Fragments arranged by size:
2590 787 567 360 270 84 63
RsrII CG'GwC_CG
Cuts at: 317 2916 317
Size: 2599 2122
SacI G_AGCT'C
Cuts at: 362 523 632 1381 1421 1735 362
Size: 161 109 749 40 314 3348
Fragments arranged by size:
3348 749 314 161 109 40
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1235 1768 1235
Size: 533 4188
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SexAI A'CCwGG_T
Cuts at: 266 266
Size: 4721
SfcI C'TryA_G
Cuts at: 33 310 484 724 856 1087 1150 1231 1291
Size: 277 174 240 132 231 63 81 60
Cuts at: 1291 1297 1764 2449 3442 3633 33
Size: 6 467 685 993 191 1121
Fragments arranged by size:
1121 993 685 467 277 240 231 191
174 132 81 63 60 6
SfoI GGC'GCC
Cuts at: 699 1588 2153 2401 699
Size: 889 565 248 3019
Fragments arranged by size:
3019 889 565 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 886 1225 1743 886
Size: 339 518 3864
Fragments arranged by size:
3864 518 339
SmlI C'TyrA_G
Cuts at: 346 598 631 745 1876 2791 3283 3545 346
Size: 252 33 114 1131 915 492 262 1522
Fragments arranged by size:
1522 1131 915 492 262 252 114 33
SnaBI TAC'GTA
Cuts at: 1105 1105
Size: 4721
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
StuI AGG'CCT
Cuts at: 449 904 1550 449
Size: 455 646 3620
Fragments arranged by size:
3620 646 455
StyI C'CwwG_G
Cuts at: 818 1855 2209 818
Size: 1037 354 3330
Fragments arranged by size:
3330 1037 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 165 280 1416 1462 2304 2437 2896 3105 165
Size: 115 1136 46 842 133 459 209 1781
Fragments arranged by size:
1781 1136 842 459 209 133 115 46
TatI w'GTAC_w
Cuts at: 377 1121 4236 377
Size: 744 3115 862
Fragments arranged by size:
3115 862 744
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 276 308 832 864 4238 4270 276
Size: 32 524 32 3374 32 727
Fragments arranged by size:
3374 727 524 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2518 2518
Size: 4721
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2216 2216
Size: 4721
XhoI C'TCGA_G
Cuts at: 346 346
Size: 4721
XmaI C'CCGG_G
Cuts at: 884 1223 1741 884
Size: 339 518 3864
Fragments arranged by size:
3864 518 339
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XmnI ZraI