Restriction map for
pF1KB9954
(Circular) (Six-base) MAPSORT of: pF1KB9954.seq Check: 321 from: 1 to: 5144
pF1KB9954 5144 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1947 1947
Size: 5144
AccI GT'mk_AC
Cuts at: 2182 2182
Size: 5144
Acc65I G'GTAC_C
Cuts at: 2160 2160
Size: 5144
AclI AA'CG_TT
Cuts at: 4984 4984
Size: 5144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 723 1024 1456 2969 3401 4148 4416 272
Size: 451 301 432 1513 432 747 268 1000
Fragments arranged by size:
1513 1000 747 451 432 432 301 268
AfeI AGC'GCT
Cuts at: 273 273
Size: 5144
AflIII A'CryG_T
Cuts at: 2496 3600 4577 2496
Size: 1104 977 3063
Fragments arranged by size:
3063 1104 977
AgeI A'CCGG_T
Cuts at: 3532 3532
Size: 5144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 335 2108 335
Size: 1773 3371
AlwNI CAG_nnn'CTG
Cuts at: 2449 4016 2449
Size: 1567 3577
ApaLI G'TGCA_C
Cuts at: 3914 3914
Size: 5144
ApoI r'AATT_y
Cuts at: 435 592 787 856 1136 1370 1458 1508 1515
Size: 157 195 69 280 234 88 50 7
Cuts at: 1515 2112 2148 4497 4753 435
Size: 597 36 2349 256 826
Fragments arranged by size:
2349 826 597 280 256 234 195 157
88 69 50 36 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 5144
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
AvaI C'yCGr_G
Cuts at: 2164 2164
Size: 5144
BamHI G'GATC_C
Cuts at: 1903 2169 1903
Size: 266 4878
BanI G'GyrC_C
Cuts at: 2160 2574 2822 2857 2160
Size: 414 248 35 4447
Fragments arranged by size:
4447 414 248 35
BanII G_rGCy'C
Cuts at: 2158 3188 2158
Size: 1030 4114
BbeI G_GCGC'C
Cuts at: 2578 2826 2578
Size: 248 4896
BbsI GAAGACnn'nnnn_
Cuts at: 122 260 892 1979 122
Size: 138 632 1087 3287
Fragments arranged by size:
3287 1087 632 138
BciVI GTATCCnnnnn_n'
Cuts at: 1716 3042 3803 5144 1716
Size: 1326 761 1341 1716
Fragments arranged by size:
1716 1341 1326 761
BclI T'GATC_A
Cuts at: 1101 1602 1917 2664 1101
Size: 501 315 747 3581
Fragments arranged by size:
3581 747 501 315
BfrBI ATG'CAT
Cuts at: 838 1078 1113 838
Size: 240 35 4869
Fragments arranged by size:
4869 240 35
BglI GCCn_nnn'nGGC
Cuts at: 212 290 212
Size: 78 5066
BglII A'GATC_T
Cuts at: 1731 2659 4696 1731
Size: 928 2037 2179
Fragments arranged by size:
2179 2037 928
BlpI GC'TnA_GC
Cuts at: 1379 2256 1379
Size: 877 4267
Bme1580I G_kGCm'C
Cuts at: 2769 2862 3918 2769
Size: 93 1056 3995
Fragments arranged by size:
3995 1056 93
BmgBI CAC'GTC
Cuts at: 694 694
Size: 5144
BmrI ACTGGGnnnn_n'
Cuts at: 2356 2437 2772 4916 2356
Size: 81 335 2144 2584
Fragments arranged by size:
2584 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 632 3511 632
Size: 2879 2265
Bpu10I CC'TnA_GC
Cuts at: 1068 1068
Size: 5144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1007 2320 3199 3691 3989 1007
Size: 1313 879 492 298 2162
Fragments arranged by size:
2162 1313 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 368 4848 368
Size: 4480 664
BsaAI yAC'GTr
Cuts at: 2497 2497
Size: 5144
BsaBI GATnn'nnATC
Cuts at: 6 998 2658 4695 6
Size: 992 1660 2037 455
Fragments arranged by size:
2037 1660 992 455
BsaHI Gr'CG_yC
Cuts at: 1299 2575 2823 3525 5021 1299
Size: 1276 248 702 1496 1422
Fragments arranged by size:
1496 1422 1276 702 248
BsaWI w'CCGG_w
Cuts at: 2333 2854 3532 3806 3953 4364 2333
Size: 521 678 274 147 411 3113
Fragments arranged by size:
3113 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 710 740 710
Size: 30 5114
BseYI C'CCAG_C
Cuts at: 381 2569 3327 3904 4400 381
Size: 2188 758 577 496 1125
Fragments arranged by size:
2188 1125 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1556 1556
Size: 5144
BsiEI CG_ry'CG
Cuts at: 104 2686 2732 3940 104
Size: 2582 46 1208 1308
Fragments arranged by size:
2582 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 519 1932 2158 2936 3126 3918 519
Size: 1413 226 778 190 792 1745
Fragments arranged by size:
1745 1413 792 778 226 190
BsmBI CGTCTCn'nnnn_
Cuts at: 775 775
Size: 5144
Bsp1286I G_dGCh'C
Cuts at: 519 1932 2158 2769 2862 2936 3126 3188 3918
Size: 1413 226 611 93 74 190 62 730
Cuts at: 3918 519
Size: 1745
Fragments arranged by size:
1745 1413 730 611 226 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4364 4364
Size: 5144
BspHI T'CATG_A
Cuts at: 345 759 1098 4320 345
Size: 414 339 3222 1169
Fragments arranged by size:
3222 1169 414 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1947 2194 2408 2710 3091 1947
Size: 247 214 302 381 4000
Fragments arranged by size:
4000 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 682 3436 3490 4974 5139 50
Size: 632 2754 54 1484 165 55
Fragments arranged by size:
2754 1484 632 165 55 54
BsrDI GCAATG_nn'
Cuts at: 300 1115 3056 300
Size: 815 1941 2388
Fragments arranged by size:
2388 1941 815
BsrFI r'CCGG_y
Cuts at: 1301 3142 3323 3532 1301
Size: 1841 181 209 2913
Fragments arranged by size:
2913 1841 209 181
BssSI C'ACGA_G
Cuts at: 3415 3773 3415
Size: 358 4786
BstAPI GCAn_nnn'nTGC
Cuts at: 2521 2521
Size: 5144
BstBI TT'CG_AA
Cuts at: 3505 3505
Size: 5144
BstEII G'GTnAC_C
Cuts at: 673 673
Size: 5144
BstYI r'GATC_y
Cuts at: 1 1731 1903 2169 2636 2659 2994 3240 4252
Size: 1730 172 266 467 23 335 246 1012
Cuts at: 4252 4338 4350 4696 1
Size: 86 12 346 449
Fragments arranged by size:
1730 1012 467 449 346 335 266 246
172 86 23 12
BtgI C'CryG_G
Cuts at: 629 629
Size: 5144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3094 3275 3438 3094
Size: 181 163 4800
Fragments arranged by size:
4800 181 163
BtsI GCAGTG_nn'
Cuts at: 2387 2387
Size: 5144
DraI TTT'AAA
Cuts at: 2109 2143 2109
Size: 34 5110
DrdI GACnn_nn'nnGTC
Cuts at: 2850 3708 2850
Size: 858 4286
EaeI y'GGCC_r
Cuts at: 2729 2903 3294 3321 4666 2729
Size: 174 391 27 1345 3207
Fragments arranged by size:
3207 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2729 2729
Size: 5144
EarI CTCTTCn'nnn_
Cuts at: 807 1047 3167 3377 807
Size: 240 2120 210 2574
Fragments arranged by size:
2574 2120 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3352 3662 3808 4954 3352
Size: 310 146 1146 3542
Fragments arranged by size:
3542 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2156 2156
Size: 5144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 723 1024 1456 2969 3401 4148 4416 272
Size: 451 301 432 1513 432 747 268 1000
Fragments arranged by size:
1513 1000 747 451 432 432 301 268
EcoO109I rG'GnC_Cy
Cuts at: 2283 2283
Size: 5144
EcoRI G'AATT_C
Cuts at: 1458 1508 2148 1458
Size: 50 640 4454
Fragments arranged by size:
4454 640 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1437 3163 1437
Size: 1726 3418
FspI TGC'GCA
Cuts at: 2925 2925
Size: 5144
HaeII r_GCGC'y
Cuts at: 275 2578 2826 3848 4827 275
Size: 2303 248 1022 979 592
Fragments arranged by size:
2303 1022 979 592 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1172 1204 1587 1619 1743 1775 2986 3016 3018
Size: 32 383 32 124 32 1211 30 2
Cuts at: 3018 3048 1172
Size: 30 3268
Fragments arranged by size:
3268 1211 383 124 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1337 1428 2183 1337
Size: 91 755 4298
Fragments arranged by size:
4298 755 91
HindIII A'AGCT_T
Cuts at: 149 149
Size: 5144
HpaI GTT'AAC
Cuts at: 1337 1337
Size: 5144
KasI G'GCGC_C
Cuts at: 2574 2822 2574
Size: 248 4896
KpnI G_GTAC'C
Cuts at: 2164 2164
Size: 5144
MfeI C'AATT_G
Cuts at: 1016 1016
Size: 5144
MluI A'CGCG_T
Cuts at: 4577 4577
Size: 5144
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 225 1042 1981 2081 3815 3999 225
Size: 817 939 100 1734 184 1370
Fragments arranged by size:
1734 1370 939 817 184 100
MscI TGG'CCA
Cuts at: 2905 2905
Size: 5144
MslI CAynn'nnrTG
Cuts at: 317 344 3133 3260 317
Size: 27 2789 127 2201
Fragments arranged by size:
2789 2201 127 27
MspA1I CmG'CkG
Cuts at: 381 805 2255 2569 2929 3942 4187 381
Size: 424 1450 314 360 1013 245 1338
Fragments arranged by size:
1450 1338 1013 424 360 314 245
NaeI GCC'GGC
Cuts at: 1303 3325 1303
Size: 2022 3122
NarI GG'CG_CC
Cuts at: 2575 2823 2575
Size: 248 4896
NgoMIV G'CCGG_C
Cuts at: 1301 3323 1301
Size: 2022 3122
NsiI A_TGCA'T
Cuts at: 840 1080 1115 840
Size: 240 35 4869
Fragments arranged by size:
4869 240 35
NspI r_CATG'y
Cuts at: 1061 2197 2697 3604 1061
Size: 1136 500 907 2601
Fragments arranged by size:
2601 1136 907 500
PasI CC'CwG_GG
Cuts at: 2001 2001
Size: 5144
PciI A'CATG_T
Cuts at: 3600 3600
Size: 5144
PmeI GTTT'AAAC
Cuts at: 2143 2143
Size: 5144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 5112
PstI C_TGCA'G
Cuts at: 237 623 2191 2876 237
Size: 386 1568 685 2505
Fragments arranged by size:
2505 1568 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5144
PvuII CAG'CTG
Cuts at: 381 805 2569 2929 381
Size: 424 1764 360 2596
Fragments arranged by size:
2596 1764 424 360
RsrII CG'GwC_CG
Cuts at: 3339 3339
Size: 5144
SacI G_AGCT'C
Cuts at: 2158 2158
Size: 5144
SalI G'TCGA_C
Cuts at: 2181 2181
Size: 5144
SapI GCTCTTCn'nnn_
Cuts at: 807 1047 3167 3377 807
Size: 240 2120 210 2574
Fragments arranged by size:
2574 2120 240 210
SbfI CC_TGCA'GG
Cuts at: 2191 2191
Size: 5144
ScaI AGT'ACT
Cuts at: 4661 4661
Size: 5144
SfcI C'TryA_G
Cuts at: 33 233 619 923 1221 2187 2872 3865 4056
Size: 200 386 304 298 966 685 993 191
Cuts at: 4056 33
Size: 1121
Fragments arranged by size:
1121 993 966 685 386 304 298 200
191
SfoI GGC'GCC
Cuts at: 2576 2824 2576
Size: 248 4896
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5144
SmaI CCC'GGG
Cuts at: 2166 2166
Size: 5144
SmlI C'TyrA_G
Cuts at: 1022 2299 3214 3706 3968 1022
Size: 1277 915 492 262 2198
Fragments arranged by size:
2198 1277 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5144
SphI G_CATG'C
Cuts at: 2197 2697 2197
Size: 500 4644
StyI C'CwwG_G
Cuts at: 170 729 828 2278 2632 170
Size: 559 99 1450 354 2682
Fragments arranged by size:
2682 1450 559 354 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 661 692 932 2727 2860 3319 3528 661
Size: 31 240 1795 133 459 209 2277
Fragments arranged by size:
2277 1795 459 240 209 133 31
TatI w'GTAC_w
Cuts at: 4659 4659
Size: 5144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 710 742 1518 1550 1938 1970 4661 4693 710
Size: 32 776 32 388 32 2691 32 1161
Fragments arranged by size:
2691 1161 776 388 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2941 2941
Size: 5144
XbaI T'CTAG_A
Cuts at: 2175 2175
Size: 5144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2639 2639
Size: 5144
XmaI C'CCGG_G
Cuts at: 2164 2164
Size: 5144
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PasI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmtI BpmI BseRI BsiWI BsmI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI NcoI NdeI NheI NotI
NruI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI