Restriction map for
pF1KB9955
(Circular) (Six-base) MAPSORT of: pF1KB9955.seq Check: 8030 from: 1 to: 4724
pF1KB9955 4724 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1530 1530
Size: 4724
AccI GT'mk_AC
Cuts at: 1762 1762
Size: 4724
Acc65I G'GTAC_C
Cuts at: 188 619 970 1740 188
Size: 431 351 770 3172
Fragments arranged by size:
3172 770 431 351
AclI AA'CG_TT
Cuts at: 4564 4564
Size: 4724
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 763 1090 1649 2549 2981 3728 3996 763
Size: 327 559 900 432 747 268 1491
Fragments arranged by size:
1491 900 747 559 432 327 268
AflIII A'CryG_T
Cuts at: 1451 2076 3180 4157 1451
Size: 625 1104 977 2018
Fragments arranged by size:
2018 1104 977 625
AgeI A'CCGG_T
Cuts at: 3112 3112
Size: 4724
AhdI GACnn_n'nnGTC
Cuts at: 292 1455 292
Size: 1163 3561
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 837 837
Size: 4724
AlwNI CAG_nnn'CTG
Cuts at: 2029 3596 2029
Size: 1567 3157
ApaI G_GGCC'C
Cuts at: 765 765
Size: 4724
ApaLI G'TGCA_C
Cuts at: 3494 3494
Size: 4724
ApoI r'AATT_y
Cuts at: 430 719 1728 4077 4333 430
Size: 289 1009 2349 256 821
Fragments arranged by size:
2349 1009 821 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4724
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
AvaI C'yCGr_G
Cuts at: 758 908 1411 1744 758
Size: 150 503 333 3738
Fragments arranged by size:
3738 503 333 150
BamHI G'GATC_C
Cuts at: 1749 1749
Size: 4724
BanI G'GyrC_C
Cuts at: 188 619 655 970 1740 2154 2402 2437 188
Size: 431 36 315 770 414 248 35 2475
Fragments arranged by size:
2475 770 431 414 315 248 36 35
BanII G_rGCy'C
Cuts at: 184 765 1118 1191 1601 1694 1738 2768 184
Size: 581 353 73 410 93 44 1030 2140
Fragments arranged by size:
2140 1030 581 410 353 93 73 44
BbeI G_GCGC'C
Cuts at: 2158 2406 2158
Size: 248 4476
BbsI GAAGACnn'nnnn_
Cuts at: 1361 1676 1361
Size: 315 4409
BbvCI CC'TCA_GC
Cuts at: 215 215
Size: 4724
BciVI GTATCCnnnnn_n'
Cuts at: 736 2622 3383 4724 736
Size: 1886 761 1341 736
Fragments arranged by size:
1886 1341 761 736
BclI T'GATC_A
Cuts at: 926 2244 926
Size: 1318 3406
BglI GCCn_nnn'nGGC
Cuts at: 1414 1414
Size: 4724
BglII A'GATC_T
Cuts at: 1275 2239 4276 1275
Size: 964 2037 1723
Fragments arranged by size:
2037 1723 964
BlpI GC'TnA_GC
Cuts at: 584 1836 584
Size: 1252 3472
Bme1580I G_kGCm'C
Cuts at: 765 2349 2442 3498 765
Size: 1584 93 1056 1991
Fragments arranged by size:
1991 1584 1056 93
BmgBI CAC'GTC
Cuts at: 1452 1452
Size: 4724
BmrI ACTGGGnnnn_n'
Cuts at: 759 946 1689 1936 2017 2352 4496 759
Size: 187 743 247 81 335 2144 987
Fragments arranged by size:
2144 987 743 335 247 187 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 379 3091 379
Size: 2712 2012
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 1555 509
Size: 1046 3678
Bpu10I CC'TnA_GC
Cuts at: 215 215
Size: 4724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1900 2779 3271 3569 1063
Size: 837 879 492 298 2218
Fragments arranged by size:
2218 879 837 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 692 823 4428 692
Size: 131 3605 988
Fragments arranged by size:
3605 988 131
BsaAI yAC'GTr
Cuts at: 2077 2077
Size: 4724
BsaBI GATnn'nnATC
Cuts at: 6 2238 4275 6
Size: 2232 2037 455
Fragments arranged by size:
2232 2037 455
BsaHI Gr'CG_yC
Cuts at: 2155 2403 3105 4601 2155
Size: 248 702 1496 2278
Fragments arranged by size:
2278 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1913 2434 3112 3386 3533 3944 1913
Size: 521 678 274 147 411 2693
Fragments arranged by size:
2693 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 689 719 689
Size: 30 4694
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 359 1126 169
Size: 190 767 3767
Fragments arranged by size:
3767 767 190
BseYI C'CCAG_C
Cuts at: 226 508 2149 2907 3484 3980 226
Size: 282 1641 758 577 496 970
Fragments arranged by size:
1641 970 758 577 496 282
BsiEI CG_ry'CG
Cuts at: 104 2266 2312 3520 104
Size: 2162 46 1208 1308
Fragments arranged by size:
2162 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 184 1191 1601 1738 2516 2706 3498 184
Size: 1007 410 137 778 190 792 1410
Fragments arranged by size:
1410 1007 792 778 410 190 137
BsiWI C'GTAC_G
Cuts at: 337 337
Size: 4724
BsmBI CGTCTCn'nnnn_
Cuts at: 323 323
Size: 4724
Bsp1286I G_dGCh'C
Cuts at: 184 765 1118 1191 1601 1694 1738 2349 2442
Size: 581 353 73 410 93 44 611 93
Cuts at: 2442 2516 2706 2768 3498 184
Size: 74 190 62 730 1410
Fragments arranged by size:
1410 730 611 581 410 353 190 93
93 74 73 62 44
BspEI T'CCGG_A
Cuts at: 3944 3944
Size: 4724
BspHI T'CATG_A
Cuts at: 1058 1601 3900 1058
Size: 543 2299 1882
Fragments arranged by size:
2299 1882 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1530 1774 1988 2290 2671 805
Size: 725 244 214 302 381 2858
Fragments arranged by size:
2858 725 381 302 244 214
BsrBI CCG'CTC
Cuts at: 50 476 3016 3070 4554 4719 50
Size: 426 2540 54 1484 165 55
Fragments arranged by size:
2540 1484 426 165 55 54
BsrDI GCAATG_nn'
Cuts at: 127 2636 127
Size: 2509 2215
BsrFI r'CCGG_y
Cuts at: 2722 2903 3112 2722
Size: 181 209 4334
Fragments arranged by size:
4334 209 181
BsrGI T'GTAC_A
Cuts at: 789 789
Size: 4724
BssSI C'ACGA_G
Cuts at: 2995 3353 2995
Size: 358 4366
BstAPI GCAn_nnn'nTGC
Cuts at: 2101 2101
Size: 4724
BstBI TT'CG_AA
Cuts at: 3085 3085
Size: 4724
BstEII G'GTnAC_C
Cuts at: 1485 1485
Size: 4724
BstXI CCAn_nnnn'nTGG
Cuts at: 184 184
Size: 4724
BstYI r'GATC_y
Cuts at: 1 1275 1749 2216 2239 2574 2820 3832 3918
Size: 1274 474 467 23 335 246 1012 86
Cuts at: 3918 3930 4276 1
Size: 12 346 449
Fragments arranged by size:
1274 1012 474 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4724
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2674 2855 3018 2674
Size: 181 163 4380
Fragments arranged by size:
4380 181 163
BtsI GCAGTG_nn'
Cuts at: 1106 1608 1967 1106
Size: 502 359 3863
Fragments arranged by size:
3863 502 359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1190 1225 1190
Size: 35 4689
DraI TTT'AAA
Cuts at: 1723 1723
Size: 4724
DrdI GACnn_nn'nnGTC
Cuts at: 1084 2430 3288 1084
Size: 1346 858 2520
Fragments arranged by size:
2520 1346 858
EaeI y'GGCC_r
Cuts at: 570 2309 2483 2874 2901 4246 570
Size: 1739 174 391 27 1345 1048
Fragments arranged by size:
1739 1345 1048 391 174 27
EagI C'GGCC_G
Cuts at: 2309 2309
Size: 4724
EarI CTCTTCn'nnn_
Cuts at: 318 542 1179 1491 2747 2957 318
Size: 224 637 312 1256 210 2085
Fragments arranged by size:
2085 1256 637 312 224 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2932 3242 3388 4534 2932
Size: 310 146 1146 3122
Fragments arranged by size:
3122 1146 310 146
EcoICRI GAG'CTC
Cuts at: 182 1189 1599 1736 182
Size: 1007 410 137 3170
Fragments arranged by size:
3170 1007 410 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 763 1090 1555 1649 2549 2981 3728 3996
Size: 254 327 465 94 900 432 747 268
Cuts at: 3996 509
Size: 1237
Fragments arranged by size:
1237 900 747 465 432 327 268 254
94
EcoO109I rG'GnC_Cy
Cuts at: 329 1623 1863 329
Size: 1294 240 3190
Fragments arranged by size:
3190 1294 240
EcoRI G'AATT_C
Cuts at: 1728 1728
Size: 4724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 4724
FspI TGC'GCA
Cuts at: 2505 2505
Size: 4724
HaeII r_GCGC'y
Cuts at: 2158 2406 3428 4407 2158
Size: 248 1022 979 2475
Fragments arranged by size:
2475 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 278 310 689 721 1267 1299 1441 1473 2566
Size: 32 379 32 546 32 142 32 1093
Cuts at: 2566 2596 2598 2628 278
Size: 30 2 30 2374
Fragments arranged by size:
2374 1093 546 379 142 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 531 1763 531
Size: 1232 3492
KasI G'GCGC_C
Cuts at: 2154 2402 2154
Size: 248 4476
KpnI G_GTAC'C
Cuts at: 192 623 974 1744 192
Size: 431 351 770 3172
Fragments arranged by size:
3172 770 431 351
MluI A'CGCG_T
Cuts at: 4157 4157
Size: 4724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3395 3579 3395
Size: 184 4540
MscI TGG'CCA
Cuts at: 2485 2485
Size: 4724
MslI CAynn'nnrTG
Cuts at: 1297 2713 2840 1297
Size: 1416 127 3181
Fragments arranged by size:
3181 1416 127
MspA1I CmG'CkG
Cuts at: 1835 2149 2509 3522 3767 1835
Size: 314 360 1013 245 2792
Fragments arranged by size:
2792 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2905 2905
Size: 4724
NarI GG'CG_CC
Cuts at: 2155 2403 2155
Size: 248 4476
NcoI C'CATG_G
Cuts at: 107 107
Size: 4724
NdeI CA'TA_TG
Cuts at: 1315 1315
Size: 4724
NgoMIV G'CCGG_C
Cuts at: 2903 2903
Size: 4724
NspI r_CATG'y
Cuts at: 1777 2277 3184 1777
Size: 500 907 3317
Fragments arranged by size:
3317 907 500
PciI A'CATG_T
Cuts at: 3180 3180
Size: 4724
PflMI CCAn_nnn'nTGG
Cuts at: 1400 1400
Size: 4724
PfoI T'CCnGG_A
Cuts at: 1664 1664
Size: 4724
PmeI GTTT'AAAC
Cuts at: 1723 1723
Size: 4724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 166 198 3863 3895 166
Size: 32 3665 32 995
Fragments arranged by size:
3665 995 32 32
PpuMI rG'GwC_Cy
Cuts at: 329 1623 329
Size: 1294 3430
PsiI TTA'TAA
Cuts at: 716 716
Size: 4724
PspOMI G'GGCC_C
Cuts at: 761 761
Size: 4724
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1417 1449 1417
Size: 32 4692
PstI C_TGCA'G
Cuts at: 816 923 1236 1771 2456 816
Size: 107 313 535 685 3084
Fragments arranged by size:
3084 685 535 313 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4724
PvuII CAG'CTG
Cuts at: 2149 2509 2149
Size: 360 4364
RsrII CG'GwC_CG
Cuts at: 2919 2919
Size: 4724
SacI G_AGCT'C
Cuts at: 184 1191 1601 1738 184
Size: 1007 410 137 3170
Fragments arranged by size:
3170 1007 410 137
SalI G'TCGA_C
Cuts at: 1761 1761
Size: 4724
SapI GCTCTTCn'nnn_
Cuts at: 1179 2747 2957 1179
Size: 1568 210 2946
Fragments arranged by size:
2946 1568 210
SbfI CC_TGCA'GG
Cuts at: 1771 1771
Size: 4724
ScaI AGT'ACT
Cuts at: 1651 4241 1651
Size: 2590 2134
SexAI A'CCwGG_T
Cuts at: 1704 1715 1704
Size: 11 4713
SfcI C'TryA_G
Cuts at: 33 812 919 1232 1554 1767 2452 3445 3636
Size: 779 107 313 322 213 685 993 191
Cuts at: 3636 33
Size: 1121
Fragments arranged by size:
1121 993 779 685 322 313 213 191
107
SfoI GGC'GCC
Cuts at: 2156 2404 2156
Size: 248 4476
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4724
SmaI CCC'GGG
Cuts at: 760 1746 760
Size: 986 3738
SmlI C'TyrA_G
Cuts at: 908 1042 1879 2794 3286 3548 908
Size: 134 837 915 492 262 2084
Fragments arranged by size:
2084 915 837 492 262 134
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4724
SphI G_CATG'C
Cuts at: 1777 2277 1777
Size: 500 4224
SspI AAT'ATT
Cuts at: 440 440
Size: 4724
StyI C'CwwG_G
Cuts at: 107 282 859 1858 2212 107
Size: 175 577 999 354 2619
Fragments arranged by size:
2619 999 577 354 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2307 2440 2899 3108 2307
Size: 133 459 209 3923
Fragments arranged by size:
3923 459 209 133
TatI w'GTAC_w
Cuts at: 789 1364 1649 4239 789
Size: 575 285 2590 1274
Fragments arranged by size:
2590 1274 575 285
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 341 373 656 688 4241 4273 341
Size: 32 283 32 3553 32 792
Fragments arranged by size:
3553 792 283 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 703 2521 703
Size: 1818 2906
XbaI T'CTAG_A
Cuts at: 1755 1755
Size: 4724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 516 1702 2219 516
Size: 1186 517 3021
Fragments arranged by size:
3021 1186 517
XhoI C'TCGA_G
Cuts at: 908 908
Size: 4724
XmaI C'CCGG_G
Cuts at: 758 1744 758
Size: 986 3738
XmnI GAAnn'nnTTC
Cuts at: 431 776 431
Size: 345 4379
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsgI BsmI BssHII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI ZraI