Restriction map for
pF1KB9957
(Circular) (Six-base) MAPSORT of: pF1KB9957.seq Check: 305 from: 1 to: 4727
pF1KB9957 4727 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1423 1494 1423
Size: 71 4656
AccI GT'mk_AC
Cuts at: 1062 1272 1765 1062
Size: 210 493 4024
Fragments arranged by size:
4024 493 210
Acc65I G'GTAC_C
Cuts at: 1365 1743 1365
Size: 378 4349
AclI AA'CG_TT
Cuts at: 1215 4567 1215
Size: 3352 1375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 302 476 532 1150 2552 2984 3731 3999
Size: 51 174 56 618 1402 432 747 268
Cuts at: 3999 251
Size: 979
Fragments arranged by size:
1402 979 747 618 432 268 174 56
51
AflIII A'CryG_T
Cuts at: 2079 3183 4160 2079
Size: 1104 977 2646
Fragments arranged by size:
2646 1104 977
AgeI A'CCGG_T
Cuts at: 209 3115 209
Size: 2906 1821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1320 1320
Size: 4727
AlwNI CAG_nnn'CTG
Cuts at: 138 1294 2032 3599 138
Size: 1156 738 1567 1266
Fragments arranged by size:
1567 1266 1156 738
ApaLI G'TGCA_C
Cuts at: 3497 3497
Size: 4727
ApoI r'AATT_y
Cuts at: 999 1151 1701 1731 4080 4336 999
Size: 152 550 30 2349 256 1390
Fragments arranged by size:
2349 1390 550 256 152 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 1747 1747
Size: 4727
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1136 1365 1743 2157 2405 2440 1136
Size: 229 378 414 248 35 3423
Fragments arranged by size:
3423 414 378 248 229 35
BanII G_rGCy'C
Cuts at: 1470 1686 1741 2771 1470
Size: 216 55 1030 3426
Fragments arranged by size:
3426 1030 216 55
BbeI G_GCGC'C
Cuts at: 1140 2161 2409 1140
Size: 1021 248 3458
Fragments arranged by size:
3458 1021 248
BbsI GAAGACnn'nnnn_
Cuts at: 1480 1488 1480
Size: 8 4719
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglII A'GATC_T
Cuts at: 507 648 2242 4279 507
Size: 141 1594 2037 955
Fragments arranged by size:
2037 1594 955 141
BlpI GC'TnA_GC
Cuts at: 1839 1839
Size: 4727
Bme1580I G_kGCm'C
Cuts at: 549 2352 2445 3501 549
Size: 1803 93 1056 1775
Fragments arranged by size:
1803 1775 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 402 1939 2020 2355 4499 402
Size: 1537 81 335 2144 630
Fragments arranged by size:
2144 1537 630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 4727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 1550 1700 814
Size: 736 150 3841
Fragments arranged by size:
3841 736 150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1903 2782 3274 3572 1903
Size: 879 492 298 3058
Fragments arranged by size:
3058 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 644 1120 1513 4431 644
Size: 476 393 2918 940
Fragments arranged by size:
2918 940 476 393
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 1137 2158 2406 3108 4604 1137
Size: 1021 248 702 1496 1260
Fragments arranged by size:
1496 1260 1021 702 248
BsaWI w'CCGG_w
Cuts at: 209 551 1916 2437 3115 3389 3536 3947 209
Size: 342 1365 521 678 274 147 411 989
Fragments arranged by size:
1365 989 678 521 411 342 274 147
BseYI C'CCAG_C
Cuts at: 1428 2152 2910 3487 3983 1428
Size: 724 758 577 496 2172
Fragments arranged by size:
2172 758 724 577 496
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3523 104
Size: 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 415 1686 1741 2519 2709 3501 415
Size: 1271 55 778 190 792 1641
Fragments arranged by size:
1641 1271 792 778 190 55
BsmI GAATG_Cn'
Cuts at: 467 467
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 415 549 1470 1686 1741 2352 2445 2519 2709
Size: 134 921 216 55 611 93 74 190
Cuts at: 2709 2771 3501 415
Size: 62 730 1641
Fragments arranged by size:
1641 921 730 611 216 190 134 93
74 62 55
BspEI T'CCGG_A
Cuts at: 551 3947 551
Size: 3396 1331
BspHI T'CATG_A
Cuts at: 735 3903 735
Size: 3168 1559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1423 1494 1777 1991 2293 2674 1423
Size: 71 283 214 302 381 3476
Fragments arranged by size:
3476 381 302 283 214 71
BsrBI CCG'CTC
Cuts at: 50 3019 3073 4557 4722 50
Size: 2969 54 1484 165 55
Fragments arranged by size:
2969 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1307 1628 2639 1307
Size: 321 1011 3395
Fragments arranged by size:
3395 1011 321
BsrFI r'CCGG_y
Cuts at: 209 2725 2906 3115 209
Size: 2516 181 209 1821
Fragments arranged by size:
2516 1821 209 181
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 2104 1168
Size: 936 3791
BstBI TT'CG_AA
Cuts at: 1698 3088 1698
Size: 1390 3337
BstXI CCAn_nnnn'nTGG
Cuts at: 1463 1463
Size: 4727
BstYI r'GATC_y
Cuts at: 1 507 648 1424 1752 2219 2242 2577 2823
Size: 506 141 776 328 467 23 335 246
Cuts at: 2823 3835 3921 3933 4279 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 776 506 467 449 346 335 328
246 141 86 23 12
BstZ17I GTA'TAC
Cuts at: 1063 1273 1063
Size: 210 4517
BtgI C'CryG_G
Cuts at: 107 107
Size: 4727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 1970 1970
Size: 4727
DraI TTT'AAA
Cuts at: 444 1726 444
Size: 1282 3445
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 2312 2486 2877 2904 4249 2312
Size: 174 391 27 1345 2790
Fragments arranged by size:
2790 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 1457 2750 2960 1457
Size: 1293 210 3224
Fragments arranged by size:
3224 1293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3245 3391 4537 2935
Size: 310 146 1146 3125
Fragments arranged by size:
3125 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1684 1739 1684
Size: 55 4672
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 302 476 532 814 1150 1550 1700 2552
Size: 51 174 56 282 336 400 150 852
Cuts at: 2552 2984 3731 3999 251
Size: 432 747 268 979
Fragments arranged by size:
979 852 747 432 400 336 282 268
174 150 56 51
EcoNI CCTnn'n_nnAGG
Cuts at: 553 553
Size: 4727
EcoO109I rG'GnC_Cy
Cuts at: 1866 1866
Size: 4727
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 1140 2161 2409 3431 4410 1140
Size: 1021 248 1022 979 1457
Fragments arranged by size:
1457 1022 1021 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1452 1484 1548 1580 2569 2599 2601 2631 1452
Size: 32 64 32 989 30 2 30 3548
Fragments arranged by size:
3548 989 64 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
HindIII A'AGCT_T
Cuts at: 284 284
Size: 4727
KasI G'GCGC_C
Cuts at: 1136 2157 2405 1136
Size: 1021 248 3458
Fragments arranged by size:
3458 1021 248
KpnI G_GTAC'C
Cuts at: 1369 1747 1369
Size: 378 4349
MfeI C'AATT_G
Cuts at: 695 695
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 1637 3398 3582 440
Size: 1197 1761 184 1585
Fragments arranged by size:
1761 1585 1197 184
MscI TGG'CCA
Cuts at: 2488 2488
Size: 4727
MslI CAynn'nnrTG
Cuts at: 193 2716 2843 193
Size: 2523 127 2077
Fragments arranged by size:
2523 2077 127
MspA1I CmG'CkG
Cuts at: 1838 2152 2512 3525 3770 1838
Size: 314 360 1013 245 2795
Fragments arranged by size:
2795 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2908 2908
Size: 4727
NarI GG'CG_CC
Cuts at: 1137 2158 2406 1137
Size: 1021 248 3458
Fragments arranged by size:
3458 1021 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4727
NgoMIV G'CCGG_C
Cuts at: 2906 2906
Size: 4727
NspI r_CATG'y
Cuts at: 537 1656 1780 2280 3187 537
Size: 1119 124 500 907 2077
Fragments arranged by size:
2077 1119 907 500 124
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 1435 1435
Size: 4727
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PsiI TTA'TAA
Cuts at: 1181 1181
Size: 4727
PstI C_TGCA'G
Cuts at: 1299 1774 2459 1299
Size: 475 685 3567
Fragments arranged by size:
3567 685 475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1686 1741 1686
Size: 55 4672
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 2750 2960 2750
Size: 210 4517
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 986 4244 986
Size: 3258 1469
SfcI C'TryA_G
Cuts at: 33 560 800 1295 1382 1770 2455 3448 3639
Size: 527 240 495 87 388 685 993 191
Cuts at: 3639 33
Size: 1121
Fragments arranged by size:
1121 993 685 527 495 388 240 191
87
SfoI GGC'GCC
Cuts at: 1138 2159 2407 1138
Size: 1021 248 3458
Fragments arranged by size:
3458 1021 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 1882 2797 3289 3551 1882
Size: 915 492 262 3058
Fragments arranged by size:
3058 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StyI C'CwwG_G
Cuts at: 107 1861 2215 107
Size: 1754 354 2619
Fragments arranged by size:
2619 1754 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2310 2443 2902 3111 2310
Size: 133 459 209 3926
Fragments arranged by size:
3926 459 209 133
TatI w'GTAC_w
Cuts at: 984 1285 4242 984
Size: 301 2957 1469
Fragments arranged by size:
2957 1469 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 4244 4276 308
Size: 32 3904 32 759
Fragments arranged by size:
3904 759 32 32
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
XmnI GAAnn'nnTTC
Cuts at: 839 839
Size: 4727
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI