Restriction map for
pF1KB9959
(Circular) (Six-base) MAPSORT of: pF1KB9959.seq Check: 7016 from: 1 to: 4727
pF1KB9959 4727 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 281 342 687 826 1569 281
Size: 61 345 139 743 3439
Fragments arranged by size:
3439 743 345 139 61
AatII G_ACGT'C
Cuts at: 962 962
Size: 4727
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 923 1743 923
Size: 820 3907
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 2552 2984 3731 3999 911
Size: 1641 432 747 268 1639
Fragments arranged by size:
1641 1639 747 432 268
AflIII A'CryG_T
Cuts at: 2079 3183 4160 2079
Size: 1104 977 2646
Fragments arranged by size:
2646 1104 977
AgeI A'CCGG_T
Cuts at: 3115 3115
Size: 4727
AleI CACnn'nnGTG
Cuts at: 781 781
Size: 4727
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1057 1089 1057
Size: 32 4695
AlwNI CAG_nnn'CTG
Cuts at: 1278 2032 3599 1278
Size: 754 1567 2406
Fragments arranged by size:
2406 1567 754
ApaLI G'TGCA_C
Cuts at: 3497 3497
Size: 4727
ApoI r'AATT_y
Cuts at: 1731 4080 4336 1731
Size: 2349 256 2122
Fragments arranged by size:
2349 2122 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4727
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 113 226 244 789 1706 1747 113
Size: 113 18 545 917 41 3093
Fragments arranged by size:
3093 917 545 113 41 18
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 250 783 923 1009 1435 1545 1743 2157 2405
Size: 533 140 86 426 110 198 414 248
Cuts at: 2405 2440 250
Size: 35 2537
Fragments arranged by size:
2537 533 426 414 248 198 140 110
86 35
BanII G_rGCy'C
Cuts at: 201 467 497 980 1042 1741 2771 201
Size: 266 30 483 62 699 1030 2157
Fragments arranged by size:
2157 1030 699 483 266 62 30
BbeI G_GCGC'C
Cuts at: 1439 2161 2409 1439
Size: 722 248 3757
Fragments arranged by size:
3757 722 248
BbsI GAAGACnn'nnnn_
Cuts at: 1307 1577 1307
Size: 270 4457
BbvCI CC'TCA_GC
Cuts at: 605 605
Size: 4727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 138 172 138
Size: 34 4693
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 481 1554 481
Size: 1073 3654
BglII A'GATC_T
Cuts at: 2242 4279 2242
Size: 2037 2690
BlpI GC'TnA_GC
Cuts at: 1127 1839 1127
Size: 712 4015
Bme1580I G_kGCm'C
Cuts at: 253 788 2352 2445 3501 253
Size: 535 1564 93 1056 1479
Fragments arranged by size:
1564 1479 1056 535 93
BmrI ACTGGGnnnn_n'
Cuts at: 452 720 1939 2020 2355 4499 452
Size: 268 1219 81 335 2144 680
Fragments arranged by size:
2144 1219 680 335 268 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 259 1386 3094 259
Size: 1127 1708 1892
Fragments arranged by size:
1892 1708 1127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 1014 1327 1372 1535 451
Size: 563 313 45 163 3643
Fragments arranged by size:
3643 563 313 163 45
Bpu10I CC'TnA_GC
Cuts at: 605 949 605
Size: 344 4383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1396 1903 2782 3274 3572 1396
Size: 507 879 492 298 2551
Fragments arranged by size:
2551 879 507 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1144 4431 1144
Size: 3287 1440
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 151 473 959 1436 2158 2406 3108 4604 151
Size: 322 486 477 722 248 702 1496 274
Fragments arranged by size:
1496 722 702 486 477 322 274 248
BsaWI w'CCGG_w
Cuts at: 193 1916 2437 3115 3389 3536 3947 193
Size: 1723 521 678 274 147 411 973
Fragments arranged by size:
1723 973 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 250 280 1603 1633 250
Size: 30 1323 30 3344
Fragments arranged by size:
3344 1323 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1306 1612 1621 1624 1306
Size: 306 9 3 4409
Fragments arranged by size:
4409 306 9 3
BseYI C'CCAG_C
Cuts at: 379 388 1043 1373 2152 2910 3487 3983 379
Size: 9 655 330 779 758 577 496 1123
Fragments arranged by size:
1123 779 758 655 577 496 330 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1077 1077
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 933 2269 2315 3523 104
Size: 829 1336 46 1208 1308
Fragments arranged by size:
1336 1308 1208 829 46
BsiHKAI G_wGCw'C
Cuts at: 442 467 1000 1403 1741 2519 2709 3501 442
Size: 25 533 403 338 778 190 792 1668
Fragments arranged by size:
1668 792 778 533 403 338 190 25
BsmBI CGTCTCn'nnnn_
Cuts at: 429 429
Size: 4727
Bsp1286I G_dGCh'C
Cuts at: 201 253 442 467 497 788 980 1000 1042
Size: 52 189 25 30 291 192 20 42
Cuts at: 1042 1403 1741 2352 2445 2519 2709 2771 3501
Size: 361 338 611 93 74 190 62 730
Cuts at: 3501 201
Size: 1427
Fragments arranged by size:
1427 730 611 361 338 291 192 190
189 93 74 62 52 42 30 25
20
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 281 342 687 826 1122 1569 1777 1991 2293
Size: 61 345 139 296 447 208 214 302
Cuts at: 2293 2674 281
Size: 381 2334
Fragments arranged by size:
2334 447 381 345 302 296 214 208
139 61
BsrBI CCG'CTC
Cuts at: 50 411 3019 3073 4557 4722 50
Size: 361 2608 54 1484 165 55
Fragments arranged by size:
2608 1484 361 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1214 2639 1214
Size: 1425 3302
BsrFI r'CCGG_y
Cuts at: 1253 2725 2906 3115 1253
Size: 1472 181 209 2865
Fragments arranged by size:
2865 1472 209 181
BsrGI T'GTAC_A
Cuts at: 296 296
Size: 4727
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 1458 2104 1278
Size: 180 646 3901
Fragments arranged by size:
3901 646 180
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstEII G'GTnAC_C
Cuts at: 309 813 309
Size: 504 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 1110 1527 1719 1110
Size: 417 192 4118
Fragments arranged by size:
4118 417 192
BstYI r'GATC_y
Cuts at: 1 686 1584 1752 2219 2242 2577 2823 3835
Size: 685 898 168 467 23 335 246 1012
Cuts at: 3835 3921 3933 4279 1
Size: 86 12 346 449
Fragments arranged by size:
1012 898 685 467 449 346 335 246
168 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1005 1005
Size: 4727
BtgI C'CryG_G
Cuts at: 107 254 489 980 1109 1235 107
Size: 147 235 491 129 126 3599
Fragments arranged by size:
3599 491 235 147 129 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1188 2677 2858 3021 1188
Size: 1489 181 163 2894
Fragments arranged by size:
2894 1489 181 163
BtsI GCAGTG_nn'
Cuts at: 1034 1067 1970 1034
Size: 33 903 3791
Fragments arranged by size:
3791 903 33
DraI TTT'AAA
Cuts at: 1726 1726
Size: 4727
DraIII CAC_nnn'GTG
Cuts at: 1062 1062
Size: 4727
DrdI GACnn_nn'nnGTC
Cuts at: 732 2433 3291 732
Size: 1701 858 2168
Fragments arranged by size:
2168 1701 858
EaeI y'GGCC_r
Cuts at: 163 353 746 930 2312 2486 2877 2904 4249
Size: 190 393 184 1382 174 391 27 1345
Cuts at: 4249 163
Size: 641
Fragments arranged by size:
1382 1345 641 393 391 190 184 174
27
EagI C'GGCC_G
Cuts at: 930 2312 930
Size: 1382 3345
EarI CTCTTCn'nnn_
Cuts at: 1384 1610 2750 2960 1384
Size: 226 1140 210 3151
Fragments arranged by size:
3151 1140 226 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3245 3391 4537 2935
Size: 310 146 1146 3125
Fragments arranged by size:
3125 1146 310 146
EcoICRI GAG'CTC
Cuts at: 465 1739 465
Size: 1274 3453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 911 1014 1327 1372 1535 2552 2984 3731
Size: 460 103 313 45 163 1017 432 747
Cuts at: 3731 3999 451
Size: 268 1179
Fragments arranged by size:
1179 1017 747 460 432 313 268 163
103 45
EcoO109I rG'GnC_Cy
Cuts at: 376 633 740 1312 1391 1501 1532 1866 376
Size: 257 107 572 79 110 31 334 3237
Fragments arranged by size:
3237 572 334 257 110 107 79 31
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 324 1439 2161 2409 3431 4410 324
Size: 1115 722 248 1022 979 641
Fragments arranged by size:
1115 1022 979 722 641 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 284 316 400 432 1639 1671 2569 2599 2601
Size: 32 84 32 1207 32 898 30 2
Cuts at: 2601 2631 284
Size: 30 2380
Fragments arranged by size:
2380 1207 898 84 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1766 1766
Size: 4727
KasI G'GCGC_C
Cuts at: 1435 2157 2405 1435
Size: 722 248 3757
Fragments arranged by size:
3757 722 248
KpnI G_GTAC'C
Cuts at: 927 1747 927
Size: 820 3907
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3398 3582 3398
Size: 184 4543
MscI TGG'CCA
Cuts at: 2488 2488
Size: 4727
MslI CAynn'nnrTG
Cuts at: 347 781 1543 1717 2716 2843 347
Size: 434 762 174 999 127 2231
Fragments arranged by size:
2231 999 762 434 174 127
MspA1I CmG'CkG
Cuts at: 121 156 168 1047 1263 1838 2152 2512 3525
Size: 35 12 879 216 575 314 360 1013
Cuts at: 3525 3770 121
Size: 245 1078
Fragments arranged by size:
1078 1013 879 575 360 314 245 216
35 12
NaeI GCC'GGC
Cuts at: 1255 2908 1255
Size: 1653 3074
NarI GG'CG_CC
Cuts at: 1436 2158 2406 1436
Size: 722 248 3757
Fragments arranged by size:
3757 722 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4727
NgoMIV G'CCGG_C
Cuts at: 1253 2906 1253
Size: 1653 3074
NspI r_CATG'y
Cuts at: 1780 2280 3187 1780
Size: 500 907 3320
Fragments arranged by size:
3320 907 500
PasI CC'CwG_GG
Cuts at: 1035 1035
Size: 4727
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PflMI CCAn_nnn'nTGG
Cuts at: 351 1062 351
Size: 711 4016
PfoI T'CCnGG_A
Cuts at: 185 288 467 185
Size: 103 179 4445
Fragments arranged by size:
4445 179 103
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PpuMI rG'GwC_Cy
Cuts at: 376 633 740 1312 1501 1532 376
Size: 257 107 572 189 31 3571
Fragments arranged by size:
3571 572 257 189 107 31
PstI C_TGCA'G
Cuts at: 1283 1774 2459 1283
Size: 491 685 3551
Fragments arranged by size:
3551 685 491
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 1047 2152 2512 1047
Size: 1105 360 3262
Fragments arranged by size:
3262 1105 360
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 467 1741 467
Size: 1274 3453
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SanDI GG'GwC_CC
Cuts at: 376 740 1532 376
Size: 364 792 3571
Fragments arranged by size:
3571 792 364
SapI GCTCTTCn'nnn_
Cuts at: 1384 2750 2960 1384
Size: 1366 210 3151
Fragments arranged by size:
3151 1366 210
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SfcI C'TryA_G
Cuts at: 33 1279 1659 1770 2455 3448 3639 33
Size: 1246 380 111 685 993 191 1121
Fragments arranged by size:
1246 1121 993 685 380 191 111
SfoI GGC'GCC
Cuts at: 1437 2159 2407 1437
Size: 722 248 3757
Fragments arranged by size:
3757 722 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 115 1708 1749 115
Size: 1593 41 3093
Fragments arranged by size:
3093 1593 41
SmlI C'TyrA_G
Cuts at: 1411 1882 2797 3289 3551 1411
Size: 471 915 492 262 2587
Fragments arranged by size:
2587 915 492 471 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
SrfI GCCC'GGGC
Cuts at: 115 115
Size: 4727
StyI C'CwwG_G
Cuts at: 107 545 779 1484 1861 2215 107
Size: 438 234 705 377 354 2619
Fragments arranged by size:
2619 705 438 377 354 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2310 2443 2902 3111 2310
Size: 133 459 209 3926
Fragments arranged by size:
3926 459 209 133
TatI w'GTAC_w
Cuts at: 296 4242 296
Size: 3946 781
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 250 282 4244 4276 250
Size: 32 3962 32 701
Fragments arranged by size:
3962 701 32 32
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1587 1758 1587
Size: 171 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XmaI C'CCGG_G
Cuts at: 113 1706 1747 113
Size: 1593 41 3093
Fragments arranged by size:
3093 1593 41
ZraI GAC'GTC
Cuts at: 960 960
Size: 4727
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BssHII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SspI StuI SwaI XhoI XmnI