Restriction map for
pF1KB9961
(Circular) (Six-base) MAPSORT of: pF1KB9961.seq Check: 6492 from: 1 to: 4721
pF1KB9961 4721 bp
With 182 enzymes: SgfI *
May 27, 2009 12:02 ..
AccI GT'mk_AC
Cuts at: 1759 1759
Size: 4721
Acc65I G'GTAC_C
Cuts at: 979 1737 979
Size: 758 3963
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2546 2978 3725 3993 1027
Size: 1519 432 747 268 1755
Fragments arranged by size:
1755 1519 747 432 268
AflIII A'CryG_T
Cuts at: 1448 2073 3177 4154 1448
Size: 625 1104 977 2015
Fragments arranged by size:
2015 1104 977 625
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AhdI GACnn_n'nnGTC
Cuts at: 393 1452 393
Size: 1059 3662
AleI CACnn'nnGTG
Cuts at: 373 844 988 373
Size: 471 144 4106
Fragments arranged by size:
4106 471 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1109 1204 1109
Size: 95 4626
AlwNI CAG_nnn'CTG
Cuts at: 682 792 879 1414 2026 3593 682
Size: 110 87 535 612 1567 1810
Fragments arranged by size:
1810 1567 612 535 110 87
ApaI G_GGCC'C
Cuts at: 349 349
Size: 4721
ApaLI G'TGCA_C
Cuts at: 178 3491 178
Size: 3313 1408
ApoI r'AATT_y
Cuts at: 1384 1725 4074 4330 1384
Size: 341 2349 256 1775
Fragments arranged by size:
2349 1775 341 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 618 905 1579 1702 1741 618
Size: 287 674 123 39 3598
Fragments arranged by size:
3598 674 287 123 39
BamHI G'GATC_C
Cuts at: 1463 1746 1463
Size: 283 4438
BanI G'GyrC_C
Cuts at: 149 528 661 979 1378 1497 1737 2151 2399
Size: 379 133 318 399 119 240 414 248
Cuts at: 2399 2434 149
Size: 35 2436
Fragments arranged by size:
2436 414 399 379 318 248 240 133
119 35
BanII G_rGCy'C
Cuts at: 167 290 349 1115 1481 1691 1735 2765 167
Size: 123 59 766 366 210 44 1030 2123
Fragments arranged by size:
2123 1030 766 366 210 123 59 44
BbeI G_GCGC'C
Cuts at: 153 532 2155 2403 153
Size: 379 1623 248 2471
Fragments arranged by size:
2471 1623 379 248
BbsI GAAGACnn'nnnn_
Cuts at: 1358 1358
Size: 4721
BbvCI CC'TCA_GC
Cuts at: 551 1066 1199 551
Size: 515 133 4073
Fragments arranged by size:
4073 515 133
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3380 4721 2619
Size: 761 1341 2619
Fragments arranged by size:
2619 1341 761
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BglI GCCn_nnn'nGGC
Cuts at: 251 277 251
Size: 26 4695
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 487 1833 487
Size: 1346 3375
Bme1580I G_kGCm'C
Cuts at: 182 349 853 1502 2346 2439 3495 182
Size: 167 504 649 844 93 1056 1408
Fragments arranged by size:
1408 1056 844 649 504 167 93
BmgBI CAC'GTC
Cuts at: 857 1449 1679 857
Size: 592 230 3899
Fragments arranged by size:
3899 592 230
BmrI ACTGGGnnnn_n'
Cuts at: 765 1686 1933 2014 2349 4493 765
Size: 921 247 81 335 2144 993
Fragments arranged by size:
2144 993 921 335 247 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 424 943 1010 1391 1684 181
Size: 243 519 67 381 293 3218
Fragments arranged by size:
3218 519 381 293 243 67
Bpu10I CC'TnA_GC
Cuts at: 551 1066 1199 551
Size: 515 133 4073
Fragments arranged by size:
4073 515 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 1897 2776 3268 3566 1150
Size: 747 879 492 298 2305
Fragments arranged by size:
2305 879 747 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 4425 643
Size: 3782 939
BsaAI yAC'GTr
Cuts at: 525 1245 2074 525
Size: 720 829 3172
Fragments arranged by size:
3172 829 720
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 150 529 2152 2400 3102 4598 150
Size: 379 1623 248 702 1496 273
Fragments arranged by size:
1623 1496 702 379 273 248
BsaWI w'CCGG_w
Cuts at: 1553 1910 2431 3109 3383 3530 3941 1553
Size: 357 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 357 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 1008 1038 745
Size: 30 233 30 4428
Fragments arranged by size:
4428 233 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 303 568 1123 1642 303
Size: 265 555 519 3382
Fragments arranged by size:
3382 555 519 265
BseYI C'CCAG_C
Cuts at: 220 256 514 933 2146 2904 3481 3977 220
Size: 36 258 419 1213 758 577 496 964
Fragments arranged by size:
1213 964 758 577 496 419 258 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1301 1573 1105
Size: 196 272 4253
Fragments arranged by size:
4253 272 196
BsiEI CG_ry'CG
Cuts at: 104 239 281 2263 2309 3517 104
Size: 135 42 1982 46 1208 1308
Fragments arranged by size:
1982 1308 1208 135 46 42
BsiHKAI G_wGCw'C
Cuts at: 182 1481 1735 2513 2703 3495 182
Size: 1299 254 778 190 792 1408
Fragments arranged by size:
1408 1299 792 778 254 190
BsiWI C'GTAC_G
Cuts at: 1241 1241
Size: 4721
Bsp1286I G_dGCh'C
Cuts at: 167 182 290 349 853 1115 1481 1502 1691
Size: 15 108 59 504 262 366 21 189
Cuts at: 1691 1735 2346 2439 2513 2703 2765 3495 167
Size: 44 611 93 74 190 62 730 1393
Fragments arranged by size:
1393 730 611 504 366 262 190 189
108 93 74 62 59 44 21 15
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 1055 3897 1055
Size: 2842 1879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1196 1658 1771 1985 2287 2668 1196
Size: 462 113 214 302 381 3249
Fragments arranged by size:
3249 462 381 302 214 113
BsrBI CCG'CTC
Cuts at: 50 435 594 1251 1545 1632 3013 3067 4551
Size: 385 159 657 294 87 1381 54 1484
Cuts at: 4551 4716 50
Size: 165 55
Fragments arranged by size:
1484 1381 657 385 294 165 159 87
55 54
BsrDI GCAATG_nn'
Cuts at: 2633 2633
Size: 4721
BsrFI r'CCGG_y
Cuts at: 355 2719 2900 3109 355
Size: 2364 181 209 1967
Fragments arranged by size:
2364 1967 209 181
BssSI C'ACGA_G
Cuts at: 634 2992 3350 634
Size: 2358 358 2005
Fragments arranged by size:
2358 2005 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2098 2098
Size: 4721
BstBI TT'CG_AA
Cuts at: 3082 3082
Size: 4721
BstXI CCAn_nnnn'nTGG
Cuts at: 375 990 1293 375
Size: 615 303 3803
Fragments arranged by size:
3803 615 303
BstYI r'GATC_y
Cuts at: 1 725 899 1463 1746 2213 2236 2571 2817
Size: 724 174 564 283 467 23 335 246
Cuts at: 2817 3829 3915 3927 4273 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 724 564 467 449 346 335 283
246 174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1230 1625 1230
Size: 395 4326
BtgI C'CryG_G
Cuts at: 107 249 275 107
Size: 142 26 4553
Fragments arranged by size:
4553 142 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 1964 1964
Size: 4721
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DraIII CAC_nnn'GTG
Cuts at: 848 848
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 414 2427 3285 414
Size: 2013 858 1850
Fragments arranged by size:
2013 1850 858
EaeI y'GGCC_r
Cuts at: 236 243 278 353 881 992 1322 1425 2306
Size: 7 35 75 528 111 330 103 881
Cuts at: 2306 2480 2871 2898 4243 236
Size: 174 391 27 1345 714
Fragments arranged by size:
1345 881 714 528 391 330 174 111
103 75 35 27 7
EagI C'GGCC_G
Cuts at: 236 278 2306 236
Size: 42 2028 2651
Fragments arranged by size:
2651 2028 42
EarI CTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1629 2929 3239 3385 4531 1629
Size: 1300 310 146 1146 1819
Fragments arranged by size:
1819 1300 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1479 1733 1479
Size: 254 4467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 424 943 1010 1027 1391 1684 2546 2978
Size: 243 519 67 17 364 293 862 432
Cuts at: 2978 3725 3993 181
Size: 747 268 909
Fragments arranged by size:
909 862 747 519 432 364 293 268
243 67 17
EcoNI CCTnn'n_nnAGG
Cuts at: 883 1042 883
Size: 159 4562
EcoO109I rG'GnC_Cy
Cuts at: 626 702 876 968 1272 1860 626
Size: 76 174 92 304 588 3487
Fragments arranged by size:
3487 588 304 174 92 76
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 4721
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 153 188 200 532 2155 2403 3425 4404 153
Size: 35 12 332 1623 248 1022 979 470
Fragments arranged by size:
1623 1022 979 470 332 248 35 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 379 411 1438 1470 2563 2593 2595 2625 379
Size: 32 1027 32 1093 30 2 30 2475
Fragments arranged by size:
2475 1093 1027 32 32 30 30 2
HincII GTy'rAC
Cuts at: 450 1760 450
Size: 1310 3411
KasI G'GCGC_C
Cuts at: 149 528 2151 2399 149
Size: 379 1623 248 2471
Fragments arranged by size:
2471 1623 379 248
KpnI G_GTAC'C
Cuts at: 983 1741 983
Size: 758 3963
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 560 1424 3392 3576 560
Size: 864 1968 184 1705
Fragments arranged by size:
1968 1705 864 184
MscI TGG'CCA
Cuts at: 883 994 2482 883
Size: 111 1488 3122
Fragments arranged by size:
3122 1488 111
MslI CAynn'nnrTG
Cuts at: 373 818 844 988 1294 2710 2837 373
Size: 445 26 144 306 1416 127 2257
Fragments arranged by size:
2257 1416 445 306 144 127 26
MspA1I CmG'CkG
Cuts at: 147 251 277 351 743 792 1203 1832 2146
Size: 104 26 74 392 49 411 629 314
Cuts at: 2146 2506 3519 3764 147
Size: 360 1013 245 1104
Fragments arranged by size:
1104 1013 629 411 392 360 314 245
104 74 49 26
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 150 529 2152 2400 150
Size: 379 1623 248 2471
Fragments arranged by size:
2471 1623 379 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4721
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NotI GC'GGCC_GC
Cuts at: 278 278
Size: 4721
NspI r_CATG'y
Cuts at: 1774 2274 3181 1774
Size: 500 907 3314
Fragments arranged by size:
3314 907 500
PasI CC'CwG_GG
Cuts at: 1507 1507
Size: 4721
PciI A'CATG_T
Cuts at: 3177 3177
Size: 4721
PflMI CCAn_nnn'nTGG
Cuts at: 1397 1397
Size: 4721
PfoI T'CCnGG_A
Cuts at: 1661 1661
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PpuMI rG'GwC_Cy
Cuts at: 626 968 1272 626
Size: 342 304 4075
Fragments arranged by size:
4075 342 304
PshAI GACnn'nnGTC
Cuts at: 585 585
Size: 4721
PspOMI G'GGCC_C
Cuts at: 345 345
Size: 4721
PstI C_TGCA'G
Cuts at: 788 1043 1655 1768 2453 788
Size: 255 612 113 685 3056
Fragments arranged by size:
3056 685 612 255 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 792 1203 2146 2506 792
Size: 411 943 360 3007
Fragments arranged by size:
3007 943 411 360
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 1481 1735 1481
Size: 254 4467
SacII CC_GC'GG
Cuts at: 252 278 252
Size: 26 4695
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1043 1655 1768 1043
Size: 612 113 3996
Fragments arranged by size:
3996 612 113
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SexAI A'CCwGG_T
Cuts at: 1307 1535 1307
Size: 228 4493
SfcI C'TryA_G
Cuts at: 33 784 1039 1651 1764 2449 3442 3633 33
Size: 751 255 612 113 685 993 191 1121
Fragments arranged by size:
1121 993 751 685 612 255 191 113
SfiI GGCCn_nnn'nGGCC
Cuts at: 251 251
Size: 4721
SfoI GGC'GCC
Cuts at: 151 530 2153 2401 151
Size: 379 1623 248 2471
Fragments arranged by size:
2471 1623 379 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1704 1743 1704
Size: 39 4682
SmlI C'TyrA_G
Cuts at: 1165 1876 2791 3283 3545 1165
Size: 711 915 492 262 2341
Fragments arranged by size:
2341 915 711 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
StuI AGG'CCT
Cuts at: 1033 1657 1033
Size: 624 4097
StyI C'CwwG_G
Cuts at: 107 131 698 884 1855 2209 107
Size: 24 567 186 971 354 2619
Fragments arranged by size:
2619 971 567 354 186 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 784 2304 2437 2896 3105 784
Size: 1520 133 459 209 2400
Fragments arranged by size:
2400 1520 459 209 133
TatI w'GTAC_w
Cuts at: 1133 1361 4236 1133
Size: 228 2875 1618
Fragments arranged by size:
2875 1618 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1008 1040 4238 4270 1008
Size: 32 3198 32 1459
Fragments arranged by size:
3198 1459 32 32
Tth111I GACn'n_nGTC
Cuts at: 322 2518 322
Size: 2196 2525
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 522 946 1293 2216 522
Size: 424 347 923 3027
Fragments arranged by size:
3027 923 424 347
XmaI C'CCGG_G
Cuts at: 1702 1741 1702
Size: 39 4682
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BaeI
BcgI BfrBI BmtI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PmlI PsiI
PspXI PsrI SanDI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI