(Circular) (Six-base) MAPSORT of: pF1KB9962.seq Check: 2748 from: 1 to: 5189 pF1KB9962 5189 bp With 182 enzymes: SgfI * September 25, 2012 15:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1550 1550 Size: 5189 AccI GT'mk_AC Cuts at: 2227 2227 Size: 5189 Acc65I G'GTAC_C Cuts at: 2205 2205 Size: 5189 AclI AA'CG_TT Cuts at: 5029 5029 Size: 5189 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 501 1252 3014 3446 4193 4461 501 Size: 751 1762 432 747 268 1229 Fragments arranged by size: 1762 1229 751 747 432 268 AflIII A'CryG_T Cuts at: 806 2541 3645 4622 806 Size: 1735 1104 977 1373 Fragments arranged by size: 1735 1373 1104 977 AgeI A'CCGG_T Cuts at: 3577 3577 Size: 5189 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 592 624 770 802 592 Size: 32 146 32 4979 Fragments arranged by size: 4979 146 32 32 AlwNI CAG_nnn'CTG Cuts at: 2083 2494 4061 2083 Size: 411 1567 3211 Fragments arranged by size: 3211 1567 411 ApaI G_GGCC'C Cuts at: 186 186 Size: 5189 ApaLI G'TGCA_C Cuts at: 3959 3959 Size: 5189 ApoI r'AATT_y Cuts at: 632 944 1713 2193 4542 4798 632 Size: 312 769 480 2349 256 1023 Fragments arranged by size: 2349 1023 769 480 312 256 AseI AT'TA_AT Cuts at: 20 20 Size: 5189 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5189 AvaI C'yCGr_G Cuts at: 1999 2209 1999 Size: 210 4979 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1352 1385 1352 Size: 33 5156 BamHI G'GATC_C Cuts at: 2214 2214 Size: 5189 BanI G'GyrC_C Cuts at: 2205 2619 2867 2902 2205 Size: 414 248 35 4492 Fragments arranged by size: 4492 414 248 35 BanII G_rGCy'C Cuts at: 186 240 325 700 1438 1531 2050 2203 3233 Size: 54 85 375 738 93 519 153 1030 Cuts at: 3233 186 Size: 2142 Fragments arranged by size: 2142 1030 738 519 375 153 93 85 54 BbeI G_GCGC'C Cuts at: 2623 2871 2623 Size: 248 4941 BbsI GAAGACnn'nnnn_ Cuts at: 1181 1181 Size: 5189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1420 1454 1420 Size: 34 5155 BciVI GTATCCnnnnn_n' Cuts at: 149 594 1899 3087 3848 5189 149 Size: 445 1305 1188 761 1341 149 Fragments arranged by size: 1341 1305 1188 761 445 149 BclI T'GATC_A Cuts at: 2709 2709 Size: 5189 BfrBI ATG'CAT Cuts at: 871 871 Size: 5189 BglII A'GATC_T Cuts at: 1521 2704 4741 1521 Size: 1183 2037 1969 Fragments arranged by size: 2037 1969 1183 BlpI GC'TnA_GC Cuts at: 2301 2301 Size: 5189 Bme1580I G_kGCm'C Cuts at: 186 2814 2907 3963 186 Size: 2628 93 1056 1412 Fragments arranged by size: 2628 1412 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1231 1563 1716 2401 2482 2817 4961 1231 Size: 332 153 685 81 335 2144 1459 Fragments arranged by size: 2144 1459 685 335 332 153 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3556 3556 Size: 5189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1049 1982 2060 1049 Size: 933 78 4178 Fragments arranged by size: 4178 933 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1113 2365 3244 3736 4034 1113 Size: 1252 879 492 298 2268 Fragments arranged by size: 2268 1252 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4893 4893 Size: 5189 BsaAI yAC'GTr Cuts at: 2542 2542 Size: 5189 BsaBI GATnn'nnATC Cuts at: 6 1277 2703 4740 6 Size: 1271 1426 2037 455 Fragments arranged by size: 2037 1426 1271 455 BsaHI Gr'CG_yC Cuts at: 2620 2868 3570 5066 2620 Size: 248 702 1496 2743 Fragments arranged by size: 2743 1496 702 248 BsaWI w'CCGG_w Cuts at: 2378 2899 3577 3851 3998 4409 2378 Size: 521 678 274 147 411 3158 Fragments arranged by size: 3158 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 298 328 770 800 1195 1225 1690 1720 298 Size: 30 442 30 395 30 465 30 3767 Fragments arranged by size: 3767 465 442 395 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 661 1056 1279 1695 661 Size: 395 223 416 4155 Fragments arranged by size: 4155 416 395 223 BseYI C'CCAG_C Cuts at: 1503 1757 2614 3372 3949 4445 1503 Size: 254 857 758 577 496 2247 Fragments arranged by size: 2247 857 758 577 496 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 285 541 285 Size: 256 4933 BsiEI CG_ry'CG Cuts at: 104 2731 2777 3985 104 Size: 2627 46 1208 1308 Fragments arranged by size: 2627 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1438 2050 2203 2981 3171 3963 1438 Size: 612 153 778 190 792 2664 Fragments arranged by size: 2664 792 778 612 190 153 Bsp1286I G_dGCh'C Cuts at: 186 240 325 700 1438 1531 2050 2203 2814 Size: 54 85 375 738 93 519 153 611 Cuts at: 2814 2907 2981 3171 3233 3963 186 Size: 93 74 190 62 730 1412 Fragments arranged by size: 1412 738 730 611 519 375 190 153 93 93 85 74 62 54 BspEI T'CCGG_A Cuts at: 4409 4409 Size: 5189 BspHI T'CATG_A Cuts at: 4365 4365 Size: 5189 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1550 2239 2453 2755 3136 1550 Size: 689 214 302 381 3603 Fragments arranged by size: 3603 689 381 302 214 BsrBI CCG'CTC Cuts at: 50 3481 3535 5019 5184 50 Size: 3431 54 1484 165 55 Fragments arranged by size: 3431 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 3101 3101 Size: 5189 BsrFI r'CCGG_y Cuts at: 875 1184 3187 3368 3577 875 Size: 309 2003 181 209 2487 Fragments arranged by size: 2487 2003 309 209 181 BsrGI T'GTAC_A Cuts at: 503 503 Size: 5189 BssSI C'ACGA_G Cuts at: 3460 3818 3460 Size: 358 4831 BstAPI GCAn_nnn'nTGC Cuts at: 2566 2566 Size: 5189 BstBI TT'CG_AA Cuts at: 3550 3550 Size: 5189 BstYI r'GATC_y Cuts at: 1 827 863 1521 1651 2214 2681 2704 3039 Size: 826 36 658 130 563 467 23 335 Cuts at: 3039 3285 4297 4383 4395 4741 1 Size: 246 1012 86 12 346 449 Fragments arranged by size: 1012 826 658 563 467 449 346 335 246 130 86 36 23 12 Bsu36I CC'TnA_GG Cuts at: 178 1346 178 Size: 1168 4021 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3139 3320 3483 3139 Size: 181 163 4845 Fragments arranged by size: 4845 181 163 BtsI GCAGTG_nn' Cuts at: 204 1510 1989 2432 204 Size: 1306 479 443 2961 Fragments arranged by size: 2961 1306 479 443 DraI TTT'AAA Cuts at: 1587 2188 1587 Size: 601 4588 DraIII CAC_nnn'GTG Cuts at: 983 983 Size: 5189 DrdI GACnn_nn'nnGTC Cuts at: 2895 3753 2895 Size: 858 4331 EaeI y'GGCC_r Cuts at: 131 2774 2948 3339 3366 4711 131 Size: 2643 174 391 27 1345 609 Fragments arranged by size: 2643 1345 609 391 174 27 EagI C'GGCC_G Cuts at: 2774 2774 Size: 5189 EarI CTCTTCn'nnn_ Cuts at: 413 1228 2055 3212 3422 413 Size: 815 827 1157 210 2180 Fragments arranged by size: 2180 1157 827 815 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3397 3707 3853 4999 3397 Size: 310 146 1146 3587 Fragments arranged by size: 3587 1146 310 146 EcoICRI GAG'CTC Cuts at: 1436 2048 2201 1436 Size: 612 153 4424 Fragments arranged by size: 4424 612 153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 501 1049 1252 1982 2060 3014 3446 4193 4461 Size: 548 203 730 78 954 432 747 268 Cuts at: 4461 501 Size: 1229 Fragments arranged by size: 1229 954 747 730 548 432 268 203 78 EcoNI CCTnn'n_nnAGG Cuts at: 1546 1546 Size: 5189 EcoO109I rG'GnC_Cy Cuts at: 182 2328 182 Size: 2146 3043 EcoRI G'AATT_C Cuts at: 944 1713 2193 944 Size: 769 480 3940 Fragments arranged by size: 3940 769 480 EcoRV GAT'ATC Cuts at: 386 386 Size: 5189 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3208 3208 Size: 5189 FspI TGC'GCA Cuts at: 2970 2970 Size: 5189 HaeII r_GCGC'y Cuts at: 2623 2871 3893 4872 2623 Size: 248 1022 979 2940 Fragments arranged by size: 2940 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 388 420 819 851 1513 1545 1675 1707 1753 Size: 32 399 32 662 32 130 32 46 Cuts at: 1753 1785 1835 1867 1897 1929 3031 3061 3063 Size: 32 50 32 30 32 1102 30 2 Cuts at: 3063 3093 388 Size: 30 2484 Fragments arranged by size: 2484 1102 662 399 130 50 46 32 32 32 32 32 32 32 30 30 30 2 HincII GTy'rAC Cuts at: 317 1727 2228 317 Size: 1410 501 3278 Fragments arranged by size: 3278 1410 501 HindIII A'AGCT_T Cuts at: 1666 1666 Size: 5189 HpaI GTT'AAC Cuts at: 317 317 Size: 5189 KasI G'GCGC_C Cuts at: 2619 2867 2619 Size: 248 4941 KpnI G_GTAC'C Cuts at: 2209 2209 Size: 5189 MluI A'CGCG_T Cuts at: 4622 4622 Size: 5189 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 166 239 1496 3860 4044 166 Size: 73 1257 2364 184 1311 Fragments arranged by size: 2364 1311 1257 184 73 MscI TGG'CCA Cuts at: 2950 2950 Size: 5189 MslI CAynn'nnrTG Cuts at: 1147 3178 3305 1147 Size: 2031 127 3031 Fragments arranged by size: 3031 2031 127 MspA1I CmG'CkG Cuts at: 445 2300 2614 2974 3987 4232 445 Size: 1855 314 360 1013 245 1402 Fragments arranged by size: 1855 1402 1013 360 314 245 NaeI GCC'GGC Cuts at: 877 3370 877 Size: 2493 2696 NarI GG'CG_CC Cuts at: 2620 2868 2620 Size: 248 4941 NgoMIV G'CCGG_C Cuts at: 875 3368 875 Size: 2493 2696 NsiI A_TGCA'T Cuts at: 873 873 Size: 5189 NspI r_CATG'y Cuts at: 810 822 875 2242 2742 3649 810 Size: 12 53 1367 500 907 2350 Fragments arranged by size: 2350 1367 907 500 53 12 PciI A'CATG_T Cuts at: 806 3645 806 Size: 2839 2350 PflMI CCAn_nnn'nTGG Cuts at: 983 983 Size: 5189 PfoI T'CCnGG_A Cuts at: 456 456 Size: 5189 PmeI GTTT'AAAC Cuts at: 2188 2188 Size: 5189 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 770 802 4328 4360 770 Size: 32 3526 32 1599 Fragments arranged by size: 3526 1599 32 32 PspOMI G'GGCC_C Cuts at: 182 182 Size: 5189 PstI C_TGCA'G Cuts at: 1368 1417 2236 2921 1368 Size: 49 819 685 3636 Fragments arranged by size: 3636 819 685 49 PvuI CG_AT'CG Cuts at: 104 104 Size: 5189 PvuII CAG'CTG Cuts at: 445 2614 2974 445 Size: 2169 360 2660 Fragments arranged by size: 2660 2169 360 RsrII CG'GwC_CG Cuts at: 3384 3384 Size: 5189 SacI G_AGCT'C Cuts at: 1438 2050 2203 1438 Size: 612 153 4424 Fragments arranged by size: 4424 612 153 SalI G'TCGA_C Cuts at: 2226 2226 Size: 5189 SapI GCTCTTCn'nnn_ Cuts at: 2055 3212 3422 2055 Size: 1157 210 3822 Fragments arranged by size: 3822 1157 210 SbfI CC_TGCA'GG Cuts at: 2236 2236 Size: 5189 ScaI AGT'ACT Cuts at: 1959 4706 1959 Size: 2747 2442 SfcI C'TryA_G Cuts at: 33 1071 1364 1413 1839 2232 2917 3910 4101 Size: 1038 293 49 426 393 685 993 191 Cuts at: 4101 33 Size: 1121 Fragments arranged by size: 1121 1038 993 685 426 393 293 191 49 SfoI GGC'GCC Cuts at: 2621 2869 2621 Size: 248 4941 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5189 SmaI CCC'GGG Cuts at: 2211 2211 Size: 5189 SmlI C'TyrA_G Cuts at: 1092 2344 3259 3751 4013 1092 Size: 1252 915 492 262 2268 Fragments arranged by size: 2268 1252 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5189 SphI G_CATG'C Cuts at: 875 2242 2742 875 Size: 1367 500 3322 Fragments arranged by size: 3322 1367 500 SspI AAT'ATT Cuts at: 748 898 1863 2113 748 Size: 150 965 250 3824 Fragments arranged by size: 3824 965 250 150 StyI C'CwwG_G Cuts at: 884 1823 2323 2677 884 Size: 939 500 354 3396 Fragments arranged by size: 3396 939 500 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2772 2905 3364 3573 2772 Size: 133 459 209 4388 Fragments arranged by size: 4388 459 209 133 TatI w'GTAC_w Cuts at: 503 1533 1957 4704 503 Size: 1030 424 2747 988 Fragments arranged by size: 2747 1030 988 424 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1690 1722 4706 4738 1690 Size: 32 2984 32 2141 Fragments arranged by size: 2984 2141 32 32 Tth111I GACn'n_nGTC Cuts at: 2986 2986 Size: 5189 XbaI T'CTAG_A Cuts at: 2220 2220 Size: 5189 XcmI CCAnnnn_n'nnnnTGG Cuts at: 980 2684 980 Size: 1704 3485 XmaI C'CCGG_G Cuts at: 2209 2209 Size: 5189 XmnI GAAnn'nnTTC Cuts at: 899 930 1337 899 Size: 31 407 4751 Fragments arranged by size: 4751 407 31 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AscI AvrII BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI FseI FspAI MfeI NcoI NdeI NheI NotI NruI PacI PasI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI