Restriction map for
pF1KB9969
(Circular) (Six-base) MAPSORT of: pF1KB9969.seq Check: 9797 from: 1 to: 4769
pF1KB9969 4769 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 226 834 226
Size: 608 4161
AccI GT'mk_AC
Cuts at: 1807 1807
Size: 4769
Acc65I G'GTAC_C
Cuts at: 1785 1785
Size: 4769
AclI AA'CG_TT
Cuts at: 4609 4609
Size: 4769
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 409 487 550 1687 2594 3026 3773 4041
Size: 41 78 63 1137 907 432 747 268
Cuts at: 4041 368
Size: 1096
Fragments arranged by size:
1137 1096 907 747 432 268 78 63
41
AflIII A'CryG_T
Cuts at: 641 2121 3225 4202 641
Size: 1480 1104 977 1208
Fragments arranged by size:
1480 1208 1104 977
AgeI A'CCGG_T
Cuts at: 3157 3157
Size: 4769
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 775 775
Size: 4769
AlwNI CAG_nnn'CTG
Cuts at: 774 1611 2074 3641 774
Size: 837 463 1567 1902
Fragments arranged by size:
1902 1567 837 463
ApaLI G'TGCA_C
Cuts at: 3539 3539
Size: 4769
ApoI r'AATT_y
Cuts at: 1773 4122 4378 1773
Size: 2349 256 2164
Fragments arranged by size:
2349 2164 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4769
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
AvaI C'yCGr_G
Cuts at: 949 1197 1403 1532 1789 949
Size: 248 206 129 257 3929
Fragments arranged by size:
3929 257 248 206 129
BamHI G'GATC_C
Cuts at: 1794 1794
Size: 4769
BanI G'GyrC_C
Cuts at: 859 942 957 1323 1522 1785 2199 2447 2482
Size: 83 15 366 199 263 414 248 35
Cuts at: 2482 859
Size: 3146
Fragments arranged by size:
3146 414 366 263 248 199 83 35
15
BanII G_rGCy'C
Cuts at: 313 1204 1783 2813 313
Size: 891 579 1030 2269
Fragments arranged by size:
2269 1030 891 579
BbeI G_GCGC'C
Cuts at: 863 1526 2203 2451 863
Size: 663 677 248 3181
Fragments arranged by size:
3181 677 663 248
BbsI GAAGACnn'nnnn_
Cuts at: 1372 1372
Size: 4769
BciVI GTATCCnnnnn_n'
Cuts at: 2667 3428 4769 2667
Size: 761 1341 2667
Fragments arranged by size:
2667 1341 761
BclI T'GATC_A
Cuts at: 2289 2289
Size: 4769
BglI GCCn_nnn'nGGC
Cuts at: 783 883 1025 1534 1552 783
Size: 100 142 509 18 4000
Fragments arranged by size:
4000 509 142 100 18
BglII A'GATC_T
Cuts at: 2284 4321 2284
Size: 2037 2732
BlpI GC'TnA_GC
Cuts at: 524 1105 1881 524
Size: 581 776 3412
Fragments arranged by size:
3412 776 581
Bme1580I G_kGCm'C
Cuts at: 947 1595 2394 2487 3543 947
Size: 648 799 93 1056 2173
Fragments arranged by size:
2173 1056 799 648 93
BmgBI CAC'GTC
Cuts at: 1659 1659
Size: 4769
BmrI ACTGGGnnnn_n'
Cuts at: 1981 2062 2397 4541 1981
Size: 81 335 2144 2209
Fragments arranged by size:
2209 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 349 459 3136 349
Size: 110 2677 1982
Fragments arranged by size:
2677 1982 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 442 652 1240 429
Size: 13 210 588 3958
Fragments arranged by size:
3958 588 210 13
Bpu10I CC'TnA_GC
Cuts at: 1288 1288
Size: 4769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1945 2824 3316 3614 1945
Size: 879 492 298 3100
Fragments arranged by size:
3100 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4473 4473
Size: 4769
BsaAI yAC'GTr
Cuts at: 642 2122 642
Size: 1480 3289
BsaBI GATnn'nnATC
Cuts at: 6 2283 4320 6
Size: 2277 2037 455
Fragments arranged by size:
2277 2037 455
BsaHI Gr'CG_yC
Cuts at: 269 503 605 860 1010 1523 2200 2448 3150
Size: 234 102 255 150 513 677 248 702
Cuts at: 3150 4646 269
Size: 1496 392
Fragments arranged by size:
1496 702 677 513 392 255 248 234
150 102
BsaWI w'CCGG_w
Cuts at: 191 1958 2479 3157 3431 3578 3989 191
Size: 1767 521 678 274 147 411 971
Fragments arranged by size:
1767 971 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 580 610 694 724 1102 1132 580
Size: 30 84 30 378 30 4217
Fragments arranged by size:
4217 378 84 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 688 1073 142
Size: 546 385 3838
Fragments arranged by size:
3838 546 385
BseYI C'CCAG_C
Cuts at: 805 871 1064 1267 2194 2952 3529 4025 805
Size: 66 193 203 927 758 577 496 1549
Fragments arranged by size:
1549 927 758 577 496 203 193 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 850 1420 850
Size: 570 4199
BsiEI CG_ry'CG
Cuts at: 104 2311 2357 3565 104
Size: 2207 46 1208 1308
Fragments arranged by size:
2207 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 994 1204 1730 1783 2561 2751 3543 994
Size: 210 526 53 778 190 792 2220
Fragments arranged by size:
2220 792 778 526 210 190 53
BsmBI CGTCTCn'nnnn_
Cuts at: 320 320
Size: 4769
Bsp1286I G_dGCh'C
Cuts at: 313 947 994 1204 1595 1730 1783 2394 2487
Size: 634 47 210 391 135 53 611 93
Cuts at: 2487 2561 2751 2813 3543 313
Size: 74 190 62 730 1539
Fragments arranged by size:
1539 730 634 611 391 210 190 135
93 74 62 53 47
BspEI T'CCGG_A
Cuts at: 191 3989 191
Size: 3798 971
BspHI T'CATG_A
Cuts at: 1001 3945 1001
Size: 2944 1825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 226 834 1819 2033 2335 2716 226
Size: 608 985 214 302 381 2279
Fragments arranged by size:
2279 985 608 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3061 3115 4599 4764 50
Size: 3011 54 1484 165 55
Fragments arranged by size:
3011 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1233 2681 1233
Size: 1448 3321
BsrFI r'CCGG_y
Cuts at: 137 939 1213 2767 2948 3157 137
Size: 802 274 1554 181 209 1749
Fragments arranged by size:
1749 1554 802 274 209 181
BssHII G'CGCG_C
Cuts at: 822 822
Size: 4769
BssSI C'ACGA_G
Cuts at: 3040 3398 3040
Size: 358 4411
BstAPI GCAn_nnn'nTGC
Cuts at: 774 837 2146 774
Size: 63 1309 3397
Fragments arranged by size:
3397 1309 63
BstBI TT'CG_AA
Cuts at: 3130 3130
Size: 4769
BstEII G'GTnAC_C
Cuts at: 1303 1303
Size: 4769
BstXI CCAn_nnnn'nTGG
Cuts at: 1267 1267
Size: 4769
BstYI r'GATC_y
Cuts at: 1 362 812 1794 2261 2284 2619 2865 3877
Size: 361 450 982 467 23 335 246 1012
Cuts at: 3877 3963 3975 4321 1
Size: 86 12 346 449
Fragments arranged by size:
1012 982 467 450 449 361 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1328 1328
Size: 4769
BtgI C'CryG_G
Cuts at: 206 716 731 1042 1205 206
Size: 510 15 311 163 3770
Fragments arranged by size:
3770 510 311 163 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 186 1352 1433 2719 2900 3063 186
Size: 1166 81 1286 181 163 1892
Fragments arranged by size:
1892 1286 1166 181 163 81
BtsI GCAGTG_nn'
Cuts at: 715 1431 2012 715
Size: 716 581 3472
Fragments arranged by size:
3472 716 581
DraI TTT'AAA
Cuts at: 1768 1768
Size: 4769
DrdI GACnn_nn'nnGTC
Cuts at: 2475 3333 2475
Size: 858 3911
EaeI y'GGCC_r
Cuts at: 293 719 848 1230 1499 1553 1586 2354 2528
Size: 426 129 382 269 54 33 768 174
Cuts at: 2528 2919 2946 4291 293
Size: 391 27 1345 771
Fragments arranged by size:
1345 771 768 426 391 382 269 174
129 54 33 27
EagI C'GGCC_G
Cuts at: 2354 2354
Size: 4769
EarI CTCTTCn'nnn_
Cuts at: 340 541 1453 1755 2792 3002 340
Size: 201 912 302 1037 210 2107
Fragments arranged by size:
2107 1037 912 302 210 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 987 1692 2977 3287 3433 4579 987
Size: 705 1285 310 146 1146 1177
Fragments arranged by size:
1285 1177 1146 705 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1781 1202
Size: 579 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 409 429 442 487 550 652 1240 1687
Size: 41 20 13 45 63 102 588 447
Cuts at: 1687 2594 3026 3773 4041 368
Size: 907 432 747 268 1096
Fragments arranged by size:
1096 907 747 588 447 432 268 102
63 45 41 20 13
EcoNI CCTnn'n_nnAGG
Cuts at: 1486 1486
Size: 4769
EcoO109I rG'GnC_Cy
Cuts at: 1574 1608 1908 1574
Size: 34 300 4435
Fragments arranged by size:
4435 300 34
EcoRI G'AATT_C
Cuts at: 1773 1773
Size: 4769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1269 2788 1269
Size: 1519 3250
FspI TGC'GCA
Cuts at: 1138 2550 1138
Size: 1412 3357
HaeII r_GCGC'y
Cuts at: 687 863 878 1526 1745 2203 2451 3473 4452
Size: 176 15 648 219 458 248 1022 979
Cuts at: 4452 687
Size: 1004
Fragments arranged by size:
1022 1004 979 648 458 248 219 176
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 354 386 1102 1134 1176 1208 1629 1661 2611
Size: 32 716 32 42 32 421 32 950
Cuts at: 2611 2641 2643 2673 354
Size: 30 2 30 2450
Fragments arranged by size:
2450 950 716 421 42 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1808 1808
Size: 4769
HindIII A'AGCT_T
Cuts at: 535 535
Size: 4769
KasI G'GCGC_C
Cuts at: 859 1522 2199 2447 859
Size: 663 677 248 3181
Fragments arranged by size:
3181 677 663 248
KpnI G_GTAC'C
Cuts at: 1789 1789
Size: 4769
MluI A'CGCG_T
Cuts at: 4202 4202
Size: 4769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 671 920 935 1145 3440 3624 671
Size: 249 15 210 2295 184 1816
Fragments arranged by size:
2295 1816 249 210 184 15
MscI TGG'CCA
Cuts at: 295 721 850 1501 2530 295
Size: 426 129 651 1029 2534
Fragments arranged by size:
2534 1029 651 426 129
MslI CAynn'nnrTG
Cuts at: 205 1733 2758 2885 205
Size: 1528 1025 127 2089
Fragments arranged by size:
2089 1528 1025 127
MspA1I CmG'CkG
Cuts at: 375 420 733 780 1559 1665 1880 2194 2554
Size: 45 313 47 779 106 215 314 360
Cuts at: 2554 3567 3812 375
Size: 1013 245 1332
Fragments arranged by size:
1332 1013 779 360 314 313 245 215
106 47 45
NaeI GCC'GGC
Cuts at: 1215 2950 1215
Size: 1735 3034
NarI GG'CG_CC
Cuts at: 860 1523 2200 2448 860
Size: 663 677 248 3181
Fragments arranged by size:
3181 677 663 248
NcoI C'CATG_G
Cuts at: 206 1205 206
Size: 999 3770
NgoMIV G'CCGG_C
Cuts at: 1213 2948 1213
Size: 1735 3034
NspI r_CATG'y
Cuts at: 1822 2322 3229 1822
Size: 500 907 3362
Fragments arranged by size:
3362 907 500
PasI CC'CwG_GG
Cuts at: 1605 1605
Size: 4769
PciI A'CATG_T
Cuts at: 3225 3225
Size: 4769
PflMI CCAn_nnn'nTGG
Cuts at: 1611 1611
Size: 4769
PfoI T'CCnGG_A
Cuts at: 815 1481 815
Size: 666 4103
PmeI GTTT'AAAC
Cuts at: 1768 1768
Size: 4769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 3908
Size: 32 4737
PshAI GACnn'nnGTC
Cuts at: 515 515
Size: 4769
PspXI vC'TCGA_Gb
Cuts at: 1197 1197
Size: 4769
PstI C_TGCA'G
Cuts at: 596 1451 1816 2501 596
Size: 855 365 685 2864
Fragments arranged by size:
2864 855 685 365
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4769
PvuII CAG'CTG
Cuts at: 375 420 1665 2194 2554 375
Size: 45 1245 529 360 2590
Fragments arranged by size:
2590 1245 529 360 45
RsrII CG'GwC_CG
Cuts at: 2964 2964
Size: 4769
SacI G_AGCT'C
Cuts at: 1204 1783 1204
Size: 579 4190
SacII CC_GC'GG
Cuts at: 734 734
Size: 4769
SalI G'TCGA_C
Cuts at: 1806 1806
Size: 4769
SapI GCTCTTCn'nnn_
Cuts at: 541 1453 2792 3002 541
Size: 912 1339 210 2308
Fragments arranged by size:
2308 1339 912 210
SbfI CC_TGCA'GG
Cuts at: 1816 1816
Size: 4769
ScaI AGT'ACT
Cuts at: 577 4286 577
Size: 3709 1060
SexAI A'CCwGG_T
Cuts at: 899 899
Size: 4769
SfcI C'TryA_G
Cuts at: 33 592 1035 1447 1578 1812 2497 3490 3681
Size: 559 443 412 131 234 685 993 191
Cuts at: 3681 33
Size: 1121
Fragments arranged by size:
1121 993 685 559 443 412 234 191
131
SfoI GGC'GCC
Cuts at: 861 1524 2201 2449 861
Size: 663 677 248 3181
Fragments arranged by size:
3181 677 663 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
SmaI CCC'GGG
Cuts at: 1405 1534 1791 1405
Size: 129 257 4383
Fragments arranged by size:
4383 257 129
SmlI C'TyrA_G
Cuts at: 1197 1924 2839 3331 3593 1197
Size: 727 915 492 262 2373
Fragments arranged by size:
2373 915 727 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4769
SphI G_CATG'C
Cuts at: 1822 2322 1822
Size: 500 4269
StuI AGG'CCT
Cuts at: 793 793
Size: 4769
StyI C'CwwG_G
Cuts at: 206 746 1205 1707 1903 2257 206
Size: 540 459 502 196 354 2718
Fragments arranged by size:
2718 540 502 459 354 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1011 2352 2485 2944 3153 1011
Size: 1341 133 459 209 2627
Fragments arranged by size:
2627 1341 459 209 133
TatI w'GTAC_w
Cuts at: 575 596 4284 575
Size: 21 3688 1060
Fragments arranged by size:
3688 1060 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 694 726 4286 4318 694
Size: 32 3560 32 1145
Fragments arranged by size:
3560 1145 32 32
Tth111I GACn'n_nGTC
Cuts at: 2566 2566
Size: 4769
XbaI T'CTAG_A
Cuts at: 1800 1800
Size: 4769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 897 2264 897
Size: 1367 3402
XhoI C'TCGA_G
Cuts at: 1197 1197
Size: 4769
XmaI C'CCGG_G
Cuts at: 1403 1532 1789 1403
Size: 129 257 4383
Fragments arranged by size:
4383 257 129
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI
BsrGI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PsiI PspOMI PsrI SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XmnI ZraI