Restriction map for
pF1KB9971
(Circular) (Six-base) MAPSORT of: pF1KB9971.seq Check: 8158 from: 1 to: 4775
pF1KB9971 4775 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 461 461
Size: 4775
AccI GT'mk_AC
Cuts at: 416 1813 416
Size: 1397 3378
Acc65I G'GTAC_C
Cuts at: 1791 1791
Size: 4775
AclI AA'CG_TT
Cuts at: 4615 4615
Size: 4775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1545 2600 3032 3779 4047 1016
Size: 529 1055 432 747 268 1744
Fragments arranged by size:
1744 1055 747 529 432 268
AflII C'TTAA_G
Cuts at: 1738 1738
Size: 4775
AflIII A'CryG_T
Cuts at: 704 2127 3231 4208 704
Size: 1423 1104 977 1271
Fragments arranged by size:
1423 1271 1104 977
AgeI A'CCGG_T
Cuts at: 3163 3163
Size: 4775
AlwNI CAG_nnn'CTG
Cuts at: 738 2080 3647 738
Size: 1342 1567 1866
Fragments arranged by size:
1866 1567 1342
ApaI G_GGCC'C
Cuts at: 344 344
Size: 4775
ApaLI G'TGCA_C
Cuts at: 3545 3545
Size: 4775
ApoI r'AATT_y
Cuts at: 784 1335 1779 4128 4384 784
Size: 551 444 2349 256 1175
Fragments arranged by size:
2349 1175 551 444 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4775
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
AvaI C'yCGr_G
Cuts at: 795 1269 1761 1795 795
Size: 474 492 34 3775
Fragments arranged by size:
3775 492 474 34
BamHI G'GATC_C
Cuts at: 1800 1800
Size: 4775
BanI G'GyrC_C
Cuts at: 1213 1765 1791 2205 2453 2488 1213
Size: 552 26 414 248 35 3500
Fragments arranged by size:
3500 552 414 248 35 26
BanII G_rGCy'C
Cuts at: 304 344 802 1102 1199 1789 2819 304
Size: 40 458 300 97 590 1030 2260
Fragments arranged by size:
2260 1030 590 458 300 97 40
BbeI G_GCGC'C
Cuts at: 1217 2209 2457 1217
Size: 992 248 3535
Fragments arranged by size:
3535 992 248
BbsI GAAGACnn'nnnn_
Cuts at: 219 1551 219
Size: 1332 3443
BbvCI CC'TCA_GC
Cuts at: 406 1567 406
Size: 1161 3614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4741
BciVI GTATCCnnnnn_n'
Cuts at: 2673 3434 4775 2673
Size: 761 1341 2673
Fragments arranged by size:
2673 1341 761
BclI T'GATC_A
Cuts at: 2295 2295
Size: 4775
BglI GCCn_nnn'nGGC
Cuts at: 476 888 476
Size: 412 4363
BglII A'GATC_T
Cuts at: 2290 4327 2290
Size: 2037 2738
BlpI GC'TnA_GC
Cuts at: 275 280 1096 1469 1887 275
Size: 5 816 373 418 3163
Fragments arranged by size:
3163 816 418 373 5
Bme1580I G_kGCm'C
Cuts at: 344 1768 2400 2493 3549 344
Size: 1424 632 93 1056 1570
Fragments arranged by size:
1570 1424 1056 632 93
BmrI ACTGGGnnnn_n'
Cuts at: 1987 2068 2403 4547 1987
Size: 81 335 2144 2215
Fragments arranged by size:
2215 2144 335 81
BmtI G_CTAG'C
Cuts at: 431 431
Size: 4775
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3142 3142
Size: 4775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 318 786 151
Size: 167 468 4140
Fragments arranged by size:
4140 468 167
Bpu10I CC'TnA_GC
Cuts at: 406 1567 406
Size: 1161 3614
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1951 2830 3322 3620 1951
Size: 879 492 298 3106
Fragments arranged by size:
3106 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1645 1669 4479 1645
Size: 24 2810 1941
Fragments arranged by size:
2810 1941 24
BsaAI yAC'GTr
Cuts at: 2128 2128
Size: 4775
BsaBI GATnn'nnATC
Cuts at: 6 1372 2289 4326 6
Size: 1366 917 2037 455
Fragments arranged by size:
2037 1366 917 455
BsaHI Gr'CG_yC
Cuts at: 1214 1688 2206 2454 3156 4652 1214
Size: 474 518 248 702 1496 1337
Fragments arranged by size:
1496 1337 702 518 474 248
BsaWI w'CCGG_w
Cuts at: 1964 2485 3163 3437 3584 3995 1964
Size: 521 678 274 147 411 2744
Fragments arranged by size:
2744 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1493 1523 1493
Size: 30 4745
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 325 325
Size: 4775
BseYI C'CCAG_C
Cuts at: 459 479 2200 2958 3535 4031 459
Size: 20 1721 758 577 496 1203
Fragments arranged by size:
1721 1203 758 577 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1189 1372 390
Size: 799 183 3793
Fragments arranged by size:
3793 799 183
BsiEI CG_ry'CG
Cuts at: 104 2317 2363 3571 104
Size: 2213 46 1208 1308
Fragments arranged by size:
2213 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 683 1187 1789 2567 2757 3549 683
Size: 504 602 778 190 792 1909
Fragments arranged by size:
1909 792 778 602 504 190
BsmI GAATG_Cn'
Cuts at: 579 1470 579
Size: 891 3884
Bsp1286I G_dGCh'C
Cuts at: 304 344 683 802 1102 1187 1199 1768 1789
Size: 40 339 119 300 85 12 569 21
Cuts at: 1789 2400 2493 2567 2757 2819 3549 304
Size: 611 93 74 190 62 730 1530
Fragments arranged by size:
1530 730 611 569 339 300 190 119
93 85 74 62 40 21 12
BspEI T'CCGG_A
Cuts at: 3995 3995
Size: 4775
BspHI T'CATG_A
Cuts at: 1364 3951 1364
Size: 2587 2188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 461 1394 1825 2039 2341 2722 126
Size: 335 933 431 214 302 381 2179
Fragments arranged by size:
2179 933 431 381 335 302 214
BsrBI CCG'CTC
Cuts at: 50 1255 3067 3121 4605 4770 50
Size: 1205 1812 54 1484 165 55
Fragments arranged by size:
1812 1484 1205 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1436 2687 1436
Size: 1251 3524
BsrFI r'CCGG_y
Cuts at: 2773 2954 3163 2773
Size: 181 209 4385
Fragments arranged by size:
4385 209 181
BssSI C'ACGA_G
Cuts at: 3046 3404 3046
Size: 358 4417
BstAPI GCAn_nnn'nTGC
Cuts at: 1587 2152 1587
Size: 565 4210
BstBI TT'CG_AA
Cuts at: 3136 3136
Size: 4775
BstXI CCAn_nnnn'nTGG
Cuts at: 216 265 292 216
Size: 49 27 4699
Fragments arranged by size:
4699 49 27
BstYI r'GATC_y
Cuts at: 1 1800 2267 2290 2625 2871 3883 3969 3981
Size: 1799 467 23 335 246 1012 86 12
Cuts at: 3981 4327 1
Size: 346 449
Fragments arranged by size:
1799 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1135 1135
Size: 4775
BtgI C'CryG_G
Cuts at: 116 344 1322 1625 116
Size: 228 978 303 3266
Fragments arranged by size:
3266 978 303 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1005 2725 2906 3069 1005
Size: 1720 181 163 2711
Fragments arranged by size:
2711 1720 181 163
BtsI GCAGTG_nn'
Cuts at: 252 2018 252
Size: 1766 3009
DraI TTT'AAA
Cuts at: 1058 1774 1058
Size: 716 4059
DrdI GACnn_nn'nnGTC
Cuts at: 2481 3339 2481
Size: 858 3917
EaeI y'GGCC_r
Cuts at: 363 434 627 1325 1419 2360 2534 2925 2952
Size: 71 193 698 94 941 174 391 27
Cuts at: 2952 4297 363
Size: 1345 841
Fragments arranged by size:
1345 941 841 698 391 193 174 94
71 27
EagI C'GGCC_G
Cuts at: 2360 2360
Size: 4775
EarI CTCTTCn'nnn_
Cuts at: 507 724 934 1070 2798 3008 507
Size: 217 210 136 1728 210 2274
Fragments arranged by size:
2274 1728 217 210 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 712 2983 3293 3439 4585 712
Size: 2271 310 146 1146 902
Fragments arranged by size:
2271 1146 902 310 146
EcoICRI GAG'CTC
Cuts at: 1787 1787
Size: 4775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 318 786 1016 1545 2600 3032 3779 4047
Size: 167 468 230 529 1055 432 747 268
Cuts at: 4047 151
Size: 879
Fragments arranged by size:
1055 879 747 529 468 432 268 230
167
EcoNI CCTnn'n_nnAGG
Cuts at: 730 1075 730
Size: 345 4430
EcoO109I rG'GnC_Cy
Cuts at: 340 463 735 1274 1914 340
Size: 123 272 539 640 3201
Fragments arranged by size:
3201 640 539 272 123
EcoRI G'AATT_C
Cuts at: 1779 1779
Size: 4775
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 186 1164 2794 186
Size: 978 1630 2167
Fragments arranged by size:
2167 1630 978
FspI TGC'GCA
Cuts at: 2556 2556
Size: 4775
HaeII r_GCGC'y
Cuts at: 378 404 859 894 1217 2209 2457 3479 4458
Size: 26 455 35 323 992 248 1022 979
Cuts at: 4458 378
Size: 695
Fragments arranged by size:
1022 992 979 695 455 323 248 35
26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2617 2647 2649 2679 2617
Size: 30 2 30 4713
Fragments arranged by size:
4713 30 30 2
HincII GTy'rAC
Cuts at: 663 1814 663
Size: 1151 3624
KasI G'GCGC_C
Cuts at: 1213 2205 2453 1213
Size: 992 248 3535
Fragments arranged by size:
3535 992 248
KpnI G_GTAC'C
Cuts at: 1795 1795
Size: 4775
MluI A'CGCG_T
Cuts at: 4208 4208
Size: 4775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3446 3630 3446
Size: 184 4591
MscI TGG'CCA
Cuts at: 365 436 1327 2536 365
Size: 71 891 1209 2604
Fragments arranged by size:
2604 1209 891 71
MslI CAynn'nnrTG
Cuts at: 214 709 1156 1327 1408 2764 2891 214
Size: 495 447 171 81 1356 127 2098
Fragments arranged by size:
2098 1356 495 447 171 127 81
MspA1I CmG'CkG
Cuts at: 346 410 459 903 1223 1627 1886 2200 2560
Size: 64 49 444 320 404 259 314 360
Cuts at: 2560 3573 3818 346
Size: 1013 245 1303
Fragments arranged by size:
1303 1013 444 404 360 320 314 259
245 64 49
NaeI GCC'GGC
Cuts at: 2956 2956
Size: 4775
NarI GG'CG_CC
Cuts at: 1214 2206 2454 1214
Size: 992 248 3535
Fragments arranged by size:
3535 992 248
NcoI C'CATG_G
Cuts at: 1322 1322
Size: 4775
NgoMIV G'CCGG_C
Cuts at: 2954 2954
Size: 4775
NheI G'CTAG_C
Cuts at: 427 427
Size: 4775
NspI r_CATG'y
Cuts at: 708 1828 2328 3235 708
Size: 1120 500 907 2248
Fragments arranged by size:
2248 1120 907 500
PciI A'CATG_T
Cuts at: 704 3231 704
Size: 2527 2248
PfoI T'CCnGG_A
Cuts at: 541 541
Size: 4775
PmeI GTTT'AAAC
Cuts at: 1774 1774
Size: 4775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 4743
PpuMI rG'GwC_Cy
Cuts at: 463 463
Size: 4775
PspOMI G'GGCC_C
Cuts at: 340 340
Size: 4775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1153 1185 1153
Size: 32 4743
PstI C_TGCA'G
Cuts at: 734 902 1822 2507 734
Size: 168 920 685 3002
Fragments arranged by size:
3002 920 685 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4775
PvuII CAG'CTG
Cuts at: 410 903 2200 2560 410
Size: 493 1297 360 2625
Fragments arranged by size:
2625 1297 493 360
RsrII CG'GwC_CG
Cuts at: 2970 2970
Size: 4775
SacI G_AGCT'C
Cuts at: 1789 1789
Size: 4775
SacII CC_GC'GG
Cuts at: 347 1628 347
Size: 1281 3494
SalI G'TCGA_C
Cuts at: 1812 1812
Size: 4775
SanDI GG'GwC_CC
Cuts at: 463 463
Size: 4775
SapI GCTCTTCn'nnn_
Cuts at: 507 724 2798 3008 507
Size: 217 2074 210 2274
Fragments arranged by size:
2274 2074 217 210
SbfI CC_TGCA'GG
Cuts at: 1822 1822
Size: 4775
ScaI AGT'ACT
Cuts at: 4292 4292
Size: 4775
SexAI A'CCwGG_T
Cuts at: 524 524
Size: 4775
SfcI C'TryA_G
Cuts at: 33 730 898 1818 2503 3496 3687 33
Size: 697 168 920 685 993 191 1121
Fragments arranged by size:
1121 993 920 697 685 191 168
SfoI GGC'GCC
Cuts at: 1215 2207 2455 1215
Size: 992 248 3535
Fragments arranged by size:
3535 992 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
SmaI CCC'GGG
Cuts at: 1271 1797 1271
Size: 526 4249
SmlI C'TyrA_G
Cuts at: 1738 1930 2845 3337 3599 1738
Size: 192 915 492 262 2914
Fragments arranged by size:
2914 915 492 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4775
SphI G_CATG'C
Cuts at: 1828 2328 1828
Size: 500 4275
StuI AGG'CCT
Cuts at: 335 355 335
Size: 20 4755
StyI C'CwwG_G
Cuts at: 360 1322 1655 1698 1909 2263 360
Size: 962 333 43 211 354 2872
Fragments arranged by size:
2872 962 354 333 211 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 471 2358 2491 2950 3159 471
Size: 1887 133 459 209 2087
Fragments arranged by size:
2087 1887 459 209 133
TatI w'GTAC_w
Cuts at: 4290 4290
Size: 4775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1493 1525 4292 4324 1493
Size: 32 2767 32 1944
Fragments arranged by size:
2767 1944 32 32
Tth111I GACn'n_nGTC
Cuts at: 2572 2572
Size: 4775
XbaI T'CTAG_A
Cuts at: 1806 1806
Size: 4775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 810 1302 2270 114
Size: 696 492 968 2619
Fragments arranged by size:
2619 968 696 492
XmaI C'CCGG_G
Cuts at: 1269 1795 1269
Size: 526 4249
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI