Restriction map for
pF1KB9972
(Circular) (Six-base) MAPSORT of: pF1KB9972.seq Check: 9399 from: 1 to: 5225
pF1KB9972 5225 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 2263 2263
Size: 5225
Acc65I G'GTAC_C
Cuts at: 2241 2241
Size: 5225
AclI AA'CG_TT
Cuts at: 5065 5065
Size: 5225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3050 3482 4229 4497 3050
Size: 432 747 268 3778
Fragments arranged by size:
3778 747 432 268
AflIII A'CryG_T
Cuts at: 2217 2577 3681 4658 2217
Size: 360 1104 977 2784
Fragments arranged by size:
2784 1104 977 360
AgeI A'CCGG_T
Cuts at: 3613 3613
Size: 5225
AleI CACnn'nnGTG
Cuts at: 1724 1894 1724
Size: 170 5055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 566 566
Size: 5225
AlwNI CAG_nnn'CTG
Cuts at: 1371 1986 2019 2109 2530 4097 1371
Size: 615 33 90 421 1567 2499
Fragments arranged by size:
2499 1567 615 421 90 33
ApaI G_GGCC'C
Cuts at: 241 241
Size: 5225
ApaLI G'TGCA_C
Cuts at: 3995 3995
Size: 5225
ApoI r'AATT_y
Cuts at: 1378 2229 4578 4834 1378
Size: 851 2349 256 1769
Fragments arranged by size:
2349 1769 851 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
AvaI C'yCGr_G
Cuts at: 777 911 2245 777
Size: 134 1334 3757
Fragments arranged by size:
3757 1334 134
AvrII C'CTAG_G
Cuts at: 2164 2164
Size: 5225
BamHI G'GATC_C
Cuts at: 2250 2250
Size: 5225
BanI G'GyrC_C
Cuts at: 168 562 1585 2241 2655 2903 2938 168
Size: 394 1023 656 414 248 35 2455
Fragments arranged by size:
2455 1023 656 414 394 248 35
BanII G_rGCy'C
Cuts at: 241 1189 1709 1853 2239 3269 241
Size: 948 520 144 386 1030 2197
Fragments arranged by size:
2197 1030 948 520 386 144
BbeI G_GCGC'C
Cuts at: 172 2659 2907 172
Size: 2487 248 2490
Fragments arranged by size:
2490 2487 248
BbvCI CC'TCA_GC
Cuts at: 680 1306 680
Size: 626 4599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 681 715 681
Size: 34 5191
BciVI GTATCCnnnnn_n'
Cuts at: 573 3123 3884 5225 573
Size: 2550 761 1341 573
Fragments arranged by size:
2550 1341 761 573
BclI T'GATC_A
Cuts at: 1373 2745 1373
Size: 1372 3853
BglI GCCn_nnn'nGGC
Cuts at: 1254 1539 1254
Size: 285 4940
BglII A'GATC_T
Cuts at: 2740 4777 2740
Size: 2037 3188
BlpI GC'TnA_GC
Cuts at: 2102 2337 2102
Size: 235 4990
Bme1580I G_kGCm'C
Cuts at: 241 1145 2850 2943 3999 241
Size: 904 1705 93 1056 1467
Fragments arranged by size:
1705 1467 1056 904 93
BmrI ACTGGGnnnn_n'
Cuts at: 225 2437 2518 2853 4997 225
Size: 2212 81 335 2144 453
Fragments arranged by size:
2212 2144 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 1203 3592 474
Size: 729 2389 2107
Fragments arranged by size:
2389 2107 729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 836 1173 1966 2053 2230 279
Size: 557 337 793 87 177 3274
Fragments arranged by size:
3274 793 557 337 177 87
Bpu10I CC'TnA_GC
Cuts at: 680 1306 680
Size: 626 4599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2401 3280 3772 4070 2401
Size: 879 492 298 3556
Fragments arranged by size:
3556 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4929 4929
Size: 5225
BsaAI yAC'GTr
Cuts at: 1140 2578 1140
Size: 1438 3787
BsaBI GATnn'nnATC
Cuts at: 6 2739 4776 6
Size: 2733 2037 455
Fragments arranged by size:
2733 2037 455
BsaHI Gr'CG_yC
Cuts at: 169 2656 2904 3606 5102 169
Size: 2487 248 702 1496 292
Fragments arranged by size:
2487 1496 702 292 248
BsaWI w'CCGG_w
Cuts at: 2414 2935 3613 3887 4034 4445 2414
Size: 521 678 274 147 411 3194
Fragments arranged by size:
3194 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 774 804 1780 1810 774
Size: 30 976 30 4189
Fragments arranged by size:
4189 976 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1057 1804 1861 2017 1057
Size: 747 57 156 4265
Fragments arranged by size:
4265 747 156 57
BseYI C'CCAG_C
Cuts at: 252 1182 1812 1875 2650 3408 3985 4481 252
Size: 930 630 63 775 758 577 496 996
Fragments arranged by size:
996 930 775 758 630 577 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 370 1078 1702 304
Size: 66 708 624 3827
Fragments arranged by size:
3827 708 624 66
BsiEI CG_ry'CG
Cuts at: 104 185 2767 2813 4021 104
Size: 81 2582 46 1208 1308
Fragments arranged by size:
2582 1308 1208 81 46
BsiHKAI G_wGCw'C
Cuts at: 1637 1709 1853 2239 3017 3207 3999 1637
Size: 72 144 386 778 190 792 2863
Fragments arranged by size:
2863 792 778 386 190 144 72
BsmBI CGTCTCn'nnnn_
Cuts at: 1753 1834 1753
Size: 81 5144
Bsp1286I G_dGCh'C
Cuts at: 241 1145 1189 1637 1709 1853 2239 2850 2943
Size: 904 44 448 72 144 386 611 93
Cuts at: 2943 3017 3207 3269 3999 241
Size: 74 190 62 730 1467
Fragments arranged by size:
1467 904 730 611 448 386 190 144
93 74 72 62 44
BspEI T'CCGG_A
Cuts at: 4445 4445
Size: 5225
BspHI T'CATG_A
Cuts at: 4401 4401
Size: 5225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2275 2489 2791 3172 2275
Size: 214 302 381 4328
Fragments arranged by size:
4328 381 302 214
BsrBI CCG'CTC
Cuts at: 50 767 1116 3517 3571 5055 5220 50
Size: 717 349 2401 54 1484 165 55
Fragments arranged by size:
2401 1484 717 349 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3137 3137
Size: 5225
BsrFI r'CCGG_y
Cuts at: 171 1582 3223 3404 3613 171
Size: 1411 1641 181 209 1783
Fragments arranged by size:
1783 1641 1411 209 181
BsrGI T'GTAC_A
Cuts at: 1202 1202
Size: 5225
BssHII G'CGCG_C
Cuts at: 112 2007 2079 112
Size: 1895 72 3258
Fragments arranged by size:
3258 1895 72
BssSI C'ACGA_G
Cuts at: 3496 3854 3496
Size: 358 4867
BstAPI GCAn_nnn'nTGC
Cuts at: 2602 2602
Size: 5225
BstBI TT'CG_AA
Cuts at: 3586 3586
Size: 5225
BstEII G'GTnAC_C
Cuts at: 790 1902 1935 790
Size: 1112 33 4080
Fragments arranged by size:
4080 1112 33
BstXI CCAn_nnnn'nTGG
Cuts at: 583 583
Size: 5225
BstYI r'GATC_y
Cuts at: 1 2250 2717 2740 3075 3321 4333 4419 4431
Size: 2249 467 23 335 246 1012 86 12
Cuts at: 4431 4777 1
Size: 346 449
Fragments arranged by size:
2249 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 1235 1931 2171 107
Size: 1128 696 240 3161
Fragments arranged by size:
3161 1128 696 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 485 3175 3356 3519 485
Size: 2690 181 163 2191
Fragments arranged by size:
2690 2191 181 163
BtsI GCAGTG_nn'
Cuts at: 517 2468 517
Size: 1951 3274
DraI TTT'AAA
Cuts at: 2224 2224
Size: 5225
DrdI GACnn_nn'nnGTC
Cuts at: 2931 3789 2931
Size: 858 4367
EaeI y'GGCC_r
Cuts at: 182 510 702 1988 2810 2984 3375 3402 4747
Size: 328 192 1286 822 174 391 27 1345
Cuts at: 4747 182
Size: 660
Fragments arranged by size:
1345 1286 822 660 391 328 192 174
27
EagI C'GGCC_G
Cuts at: 182 2810 182
Size: 2628 2597
EarI CTCTTCn'nnn_
Cuts at: 599 1687 3248 3458 599
Size: 1088 1561 210 2366
Fragments arranged by size:
2366 1561 1088 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 993 3433 3743 3889 5035 993
Size: 2440 310 146 1146 1183
Fragments arranged by size:
2440 1183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1707 1851 2237 1707
Size: 144 386 4695
Fragments arranged by size:
4695 386 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 836 1173 1966 2053 2230 3050 3482 4229
Size: 557 337 793 87 177 820 432 747
Cuts at: 4229 4497 279
Size: 268 1007
Fragments arranged by size:
1007 820 793 747 557 432 337 268
177 87
EcoO109I rG'GnC_Cy
Cuts at: 238 1808 2205 2364 238
Size: 1570 397 159 3099
Fragments arranged by size:
3099 1570 397 159
EcoRI G'AATT_C
Cuts at: 2229 2229
Size: 5225
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 3244
Size: 5225
FspI TGC'GCA
Cuts at: 1975 3006 1975
Size: 1031 4194
HaeII r_GCGC'y
Cuts at: 130 172 178 1531 2659 2907 3929 4908 130
Size: 42 6 1353 1128 248 1022 979 447
Fragments arranged by size:
1353 1128 1022 979 447 248 42 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 598 630 740 772 1792 1813 1824 1845 3067
Size: 32 110 32 1020 21 11 21 1222
Cuts at: 3067 3097 3099 3129 598
Size: 30 2 30 2694
Fragments arranged by size:
2694 1222 1020 110 32 32 30 30
21 21 11 2
HincII GTy'rAC
Cuts at: 2264 2264
Size: 5225
HindIII A'AGCT_T
Cuts at: 394 836 394
Size: 442 4783
KasI G'GCGC_C
Cuts at: 168 2655 2903 168
Size: 2487 248 2490
Fragments arranged by size:
2490 2487 248
KpnI G_GTAC'C
Cuts at: 2245 2245
Size: 5225
MluI A'CGCG_T
Cuts at: 4658 4658
Size: 5225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 641 908 1935 3896 4080 641
Size: 267 1027 1961 184 1786
Fragments arranged by size:
1961 1786 1027 267 184
MscI TGG'CCA
Cuts at: 1990 2986 1990
Size: 996 4229
MslI CAynn'nnrTG
Cuts at: 1003 1724 1771 1894 3214 3341 1003
Size: 721 47 123 1320 127 2887
Fragments arranged by size:
2887 1320 721 127 123 47
MspA1I CmG'CkG
Cuts at: 1986 2076 2106 2336 2650 3010 4023 4268 1986
Size: 90 30 230 314 360 1013 245 2943
Fragments arranged by size:
2943 1013 360 314 245 230 90 30
NaeI GCC'GGC
Cuts at: 173 1584 3406 173
Size: 1411 1822 1992
Fragments arranged by size:
1992 1822 1411
NarI GG'CG_CC
Cuts at: 169 2656 2904 169
Size: 2487 248 2490
Fragments arranged by size:
2490 2487 248
NcoI C'CATG_G
Cuts at: 107 1931 2171 107
Size: 1824 240 3161
Fragments arranged by size:
3161 1824 240
NgoMIV G'CCGG_C
Cuts at: 171 1582 3404 171
Size: 1411 1822 1992
Fragments arranged by size:
1992 1822 1411
NspI r_CATG'y
Cuts at: 1578 1980 2278 2778 3685 1578
Size: 402 298 500 907 3118
Fragments arranged by size:
3118 907 500 402 298
PciI A'CATG_T
Cuts at: 3681 3681
Size: 5225
PfoI T'CCnGG_A
Cuts at: 575 656 1349 575
Size: 81 693 4451
Fragments arranged by size:
4451 693 81
PmeI GTTT'AAAC
Cuts at: 2224 2224
Size: 5225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 4364
Size: 32 5193
PpuMI rG'GwC_Cy
Cuts at: 1808 1808
Size: 5225
PspOMI G'GGCC_C
Cuts at: 237 237
Size: 5225
PstI C_TGCA'G
Cuts at: 671 1325 2024 2272 2957 671
Size: 654 699 248 685 2939
Fragments arranged by size:
2939 699 685 654 248
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5225
PvuII CAG'CTG
Cuts at: 1986 2076 2106 2650 3010 1986
Size: 90 30 544 360 4201
Fragments arranged by size:
4201 544 360 90 30
RsrII CG'GwC_CG
Cuts at: 3420 3420
Size: 5225
SacI G_AGCT'C
Cuts at: 1709 1853 2239 1709
Size: 144 386 4695
Fragments arranged by size:
4695 386 144
SalI G'TCGA_C
Cuts at: 2262 2262
Size: 5225
SapI GCTCTTCn'nnn_
Cuts at: 599 3248 3458 599
Size: 2649 210 2366
Fragments arranged by size:
2649 2366 210
SbfI CC_TGCA'GG
Cuts at: 2272 2272
Size: 5225
ScaI AGT'ACT
Cuts at: 4742 4742
Size: 5225
SfcI C'TryA_G
Cuts at: 33 667 1321 2020 2268 2953 3946 4137 33
Size: 634 654 699 248 685 993 191 1121
Fragments arranged by size:
1121 993 699 685 654 634 248 191
SfoI GGC'GCC
Cuts at: 170 2657 2905 170
Size: 2487 248 2490
Fragments arranged by size:
2490 2487 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5225
SgrAI Cr'CCGG_yG
Cuts at: 171 171
Size: 5225
SmaI CCC'GGG
Cuts at: 779 913 2247 779
Size: 134 1334 3757
Fragments arranged by size:
3757 1334 134
SmlI C'TyrA_G
Cuts at: 2380 3295 3787 4049 2380
Size: 915 492 262 3556
Fragments arranged by size:
3556 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5225
SphI G_CATG'C
Cuts at: 1578 1980 2278 2778 1578
Size: 402 298 500 4025
Fragments arranged by size:
4025 500 402 298
StuI AGG'CCT
Cuts at: 652 652
Size: 5225
StyI C'CwwG_G
Cuts at: 107 476 1016 1229 1931 2164 2171 2359 2713
Size: 369 540 213 702 233 7 188 354
Cuts at: 2713 107
Size: 2619
Fragments arranged by size:
2619 702 540 369 354 233 213 188
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1936 2808 2941 3400 3609 1936
Size: 872 133 459 209 3552
Fragments arranged by size:
3552 872 459 209 133
TatI w'GTAC_w
Cuts at: 422 935 962 1202 4740 422
Size: 513 27 240 3538 907
Fragments arranged by size:
3538 907 513 240 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 4742 4774 865
Size: 32 3845 32 1316
Fragments arranged by size:
3845 1316 32 32
Tth111I GACn'n_nGTC
Cuts at: 1768 3022 1768
Size: 1254 3971
XbaI T'CTAG_A
Cuts at: 2256 2256
Size: 5225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 474 1005 1623 2720 231
Size: 243 531 618 1097 2736
Fragments arranged by size:
2736 1097 618 531 243
XmaI C'CCGG_G
Cuts at: 777 911 2245 777
Size: 134 1334 3757
Fragments arranged by size:
3757 1334 134
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI BaeI
BbsI BfrBI BmgBI BmtI BsiWI BsmI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI