Restriction map for
pF1KB9973
(Circular) (Six-base) MAPSORT of: pF1KB9973.seq Check: 311 from: 1 to: 4775
pF1KB9973 4775 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 257 886 1613 257
Size: 629 727 3419
Fragments arranged by size:
3419 727 629
AccI GT'mk_AC
Cuts at: 317 1813 317
Size: 1496 3279
Acc65I G'GTAC_C
Cuts at: 1791 1791
Size: 4775
AclI AA'CG_TT
Cuts at: 4615 4615
Size: 4775
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 484 2600 3032 3779 4047 387
Size: 97 2116 432 747 268 1115
Fragments arranged by size:
2116 1115 747 432 268 97
AflII C'TTAA_G
Cuts at: 352 352
Size: 4775
AflIII A'CryG_T
Cuts at: 2127 3231 4208 2127
Size: 1104 977 2694
Fragments arranged by size:
2694 1104 977
AgeI A'CCGG_T
Cuts at: 3163 3163
Size: 4775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1426 1426
Size: 4775
AlwNI CAG_nnn'CTG
Cuts at: 258 267 543 828 2080 3647 258
Size: 9 276 285 1252 1567 1386
Fragments arranged by size:
1567 1386 1252 285 276 9
ApaI G_GGCC'C
Cuts at: 1222 1222
Size: 4775
ApaLI G'TGCA_C
Cuts at: 3545 3545
Size: 4775
ApoI r'AATT_y
Cuts at: 1779 4128 4384 1779
Size: 2349 256 2170
Fragments arranged by size:
2349 2170 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4775
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
AvaI C'yCGr_G
Cuts at: 973 1398 1795 973
Size: 425 397 3953
Fragments arranged by size:
3953 425 397
BamHI G'GATC_C
Cuts at: 840 1567 1800 840
Size: 727 233 3815
Fragments arranged by size:
3815 727 233
BanI G'GyrC_C
Cuts at: 121 1611 1791 2205 2453 2488 121
Size: 1490 180 414 248 35 2408
Fragments arranged by size:
2408 1490 414 248 180 35
BanII G_rGCy'C
Cuts at: 160 1118 1222 1462 1789 2819 160
Size: 958 104 240 327 1030 2116
Fragments arranged by size:
2116 1030 958 327 240 104
BbeI G_GCGC'C
Cuts at: 125 2209 2457 125
Size: 2084 248 2443
Fragments arranged by size:
2443 2084 248
BbsI GAAGACnn'nnnn_
Cuts at: 1270 1590 1270
Size: 320 4455
BbvCI CC'TCA_GC
Cuts at: 1345 1345
Size: 4775
BciVI GTATCCnnnnn_n'
Cuts at: 2673 3434 4775 2673
Size: 761 1341 2673
Fragments arranged by size:
2673 1341 761
BclI T'GATC_A
Cuts at: 2295 2295
Size: 4775
BglI GCCn_nnn'nGGC
Cuts at: 760 1340 760
Size: 580 4195
BglII A'GATC_T
Cuts at: 2290 4327 2290
Size: 2037 2738
BlpI GC'TnA_GC
Cuts at: 1370 1887 1370
Size: 517 4258
Bme1580I G_kGCm'C
Cuts at: 1009 1222 1616 2400 2493 3549 1009
Size: 213 394 784 93 1056 2235
Fragments arranged by size:
2235 1056 784 394 213 93
BmrI ACTGGGnnnn_n'
Cuts at: 901 1610 1987 2068 2403 4547 901
Size: 709 377 81 335 2144 1129
Fragments arranged by size:
2144 1129 709 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 819 1326 1587 3142 819
Size: 507 261 1555 2452
Fragments arranged by size:
2452 1555 507 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 565 627 1055 1068 1447 1557 1570 520
Size: 45 62 428 13 379 110 13 3725
Fragments arranged by size:
3725 428 379 110 62 45 13 13
Bpu10I CC'TnA_GC
Cuts at: 1345 1345
Size: 4775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1951 2830 3322 3620 1951
Size: 879 492 298 3106
Fragments arranged by size:
3106 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 280 895 1286 4479 280
Size: 615 391 3193 576
Fragments arranged by size:
3193 615 576 391
BsaAI yAC'GTr
Cuts at: 2128 2128
Size: 4775
BsaBI GATnn'nnATC
Cuts at: 6 2289 4326 6
Size: 2283 2037 455
Fragments arranged by size:
2283 2037 455
BsaHI Gr'CG_yC
Cuts at: 122 2206 2454 3156 4652 122
Size: 2084 248 702 1496 245
Fragments arranged by size:
2084 1496 702 248 245
BsaWI w'CCGG_w
Cuts at: 1964 2485 3163 3437 3584 3995 1964
Size: 521 678 274 147 411 2744
Fragments arranged by size:
2744 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 277 307 277
Size: 30 4745
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 1386 1452 1470 220
Size: 1166 66 18 3525
Fragments arranged by size:
3525 1166 66 18
BseYI C'CCAG_C
Cuts at: 153 1126 2200 2958 3535 4031 153
Size: 973 1074 758 577 496 897
Fragments arranged by size:
1074 973 897 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 814 457
Size: 357 4418
BsiEI CG_ry'CG
Cuts at: 104 2317 2363 3571 104
Size: 2213 46 1208 1308
Fragments arranged by size:
2213 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 877 1444 1789 2567 2757 3549 877
Size: 567 345 778 190 792 2103
Fragments arranged by size:
2103 792 778 567 345 190
BsmI GAATG_Cn'
Cuts at: 643 643
Size: 4775
Bsp1286I G_dGCh'C
Cuts at: 160 877 1009 1118 1222 1444 1462 1616 1789
Size: 717 132 109 104 222 18 154 173
Cuts at: 1789 2400 2493 2567 2757 2819 3549 160
Size: 611 93 74 190 62 730 1386
Fragments arranged by size:
1386 730 717 611 222 190 173 154
132 109 104 93 74 62 18
BspEI T'CCGG_A
Cuts at: 3995 3995
Size: 4775
BspHI T'CATG_A
Cuts at: 3951 3951
Size: 4775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 257 886 1613 1825 2039 2341 2722 257
Size: 629 727 212 214 302 381 2310
Fragments arranged by size:
2310 727 629 381 302 214 212
BsrBI CCG'CTC
Cuts at: 50 1699 3067 3121 4605 4770 50
Size: 1649 1368 54 1484 165 55
Fragments arranged by size:
1649 1484 1368 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1007 2687 1007
Size: 1680 3095
BsrFI r'CCGG_y
Cuts at: 1182 2773 2954 3163 1182
Size: 1591 181 209 2794
Fragments arranged by size:
2794 1591 209 181
BssSI C'ACGA_G
Cuts at: 291 3046 3404 291
Size: 2755 358 1662
Fragments arranged by size:
2755 1662 358
BstAPI GCAn_nnn'nTGC
Cuts at: 258 617 828 2152 258
Size: 359 211 1324 2881
Fragments arranged by size:
2881 1324 359 211
BstBI TT'CG_AA
Cuts at: 3136 3136
Size: 4775
BstEII G'GTnAC_C
Cuts at: 657 657
Size: 4775
BstXI CCAn_nnnn'nTGG
Cuts at: 235 235
Size: 4775
BstYI r'GATC_y
Cuts at: 1 336 840 1319 1567 1800 2267 2290 2625
Size: 335 504 479 248 233 467 23 335
Cuts at: 2625 2871 3883 3969 3981 4327 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 504 479 467 449 346 335 335
248 246 233 86 23 12
BtgI C'CryG_G
Cuts at: 749 749
Size: 4775
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 411 2725 2906 3069 411
Size: 2314 181 163 2117
Fragments arranged by size:
2314 2117 181 163
BtsI GCAGTG_nn'
Cuts at: 711 855 1327 2018 711
Size: 144 472 691 3468
Fragments arranged by size:
3468 691 472 144
DraI TTT'AAA
Cuts at: 1774 1774
Size: 4775
DraIII CAC_nnn'GTG
Cuts at: 837 837
Size: 4775
DrdI GACnn_nn'nnGTC
Cuts at: 2481 3339 2481
Size: 858 3917
EaeI y'GGCC_r
Cuts at: 862 1236 1637 2360 2534 2925 2952 4297 862
Size: 374 401 723 174 391 27 1345 1340
Fragments arranged by size:
1345 1340 723 401 391 374 174 27
EagI C'GGCC_G
Cuts at: 2360 2360
Size: 4775
EarI CTCTTCn'nnn_
Cuts at: 460 485 2798 3008 460
Size: 25 2313 210 2227
Fragments arranged by size:
2313 2227 210 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1689 2983 3293 3439 4585 1689
Size: 1294 310 146 1146 1879
Fragments arranged by size:
1879 1294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1787 1787
Size: 4775
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 387 484 520 565 627 1055 1068 1447 1557
Size: 97 36 45 62 428 13 379 110
Cuts at: 1557 1570 2600 3032 3779 4047 387
Size: 13 1030 432 747 268 1115
Fragments arranged by size:
1115 1030 747 432 428 379 268 110
97 62 45 36 13 13
EcoNI CCTnn'n_nnAGG
Cuts at: 355 589 1209 1738 355
Size: 234 620 529 3392
Fragments arranged by size:
3392 620 529 234
EcoO109I rG'GnC_Cy
Cuts at: 962 1017 1219 1341 1505 1914 962
Size: 55 202 122 164 409 3823
Fragments arranged by size:
3823 409 202 164 122 55
EcoRI G'AATT_C
Cuts at: 1779 1779
Size: 4775
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 489 579 2794 489
Size: 90 2215 2470
Fragments arranged by size:
2470 2215 90
FspI TGC'GCA
Cuts at: 2556 2556
Size: 4775
HaeII r_GCGC'y
Cuts at: 125 135 2209 2457 3479 4458 125
Size: 10 2074 248 1022 979 442
Fragments arranged by size:
2074 1022 979 442 248 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 261 293 700 732 1257 1261 1289
Size: 32 33 32 407 32 525 4 28
Cuts at: 1289 1293 1543 1575 2617 2647 2649 2679 196
Size: 4 250 32 1042 30 2 30 2292
Fragments arranged by size:
2292 1042 525 407 250 33 32 32
32 32 30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 1814 1814
Size: 4775
KasI G'GCGC_C
Cuts at: 121 2205 2453 121
Size: 2084 248 2443
Fragments arranged by size:
2443 2084 248
KpnI G_GTAC'C
Cuts at: 1795 1795
Size: 4775
MluI A'CGCG_T
Cuts at: 4208 4208
Size: 4775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 1623 3446 3630 194
Size: 1429 1823 184 1339
Fragments arranged by size:
1823 1429 1339 184
MscI TGG'CCA
Cuts at: 1238 2536 1238
Size: 1298 3477
MslI CAynn'nnrTG
Cuts at: 2764 2891 2764
Size: 127 4648
MspA1I CmG'CkG
Cuts at: 264 441 861 1886 2200 2560 3573 3818 264
Size: 177 420 1025 314 360 1013 245 1221
Fragments arranged by size:
1221 1025 1013 420 360 314 245 177
NaeI GCC'GGC
Cuts at: 1184 2956 1184
Size: 1772 3003
NarI GG'CG_CC
Cuts at: 122 2206 2454 122
Size: 2084 248 2443
Fragments arranged by size:
2443 2084 248
NcoI C'CATG_G
Cuts at: 749 749
Size: 4775
NgoMIV G'CCGG_C
Cuts at: 1182 2954 1182
Size: 1772 3003
NspI r_CATG'y
Cuts at: 1107 1828 2328 3235 1107
Size: 721 500 907 2647
Fragments arranged by size:
2647 907 721 500
PasI CC'CwG_GG
Cuts at: 1211 1338 1211
Size: 127 4648
PciI A'CATG_T
Cuts at: 3231 3231
Size: 4775
PflMI CCAn_nnn'nTGG
Cuts at: 149 543 654 1608 149
Size: 394 111 954 3316
Fragments arranged by size:
3316 954 394 111
PfoI T'CCnGG_A
Cuts at: 1586 1586
Size: 4775
PmeI GTTT'AAAC
Cuts at: 1774 1774
Size: 4775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 4743
PpuMI rG'GwC_Cy
Cuts at: 1017 1017
Size: 4775
PspOMI G'GGCC_C
Cuts at: 1218 1218
Size: 4775
PstI C_TGCA'G
Cuts at: 254 263 446 1822 2507 254
Size: 9 183 1376 685 2522
Fragments arranged by size:
2522 1376 685 183 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4775
PvuII CAG'CTG
Cuts at: 441 2200 2560 441
Size: 1759 360 2656
Fragments arranged by size:
2656 1759 360
RsrII CG'GwC_CG
Cuts at: 2970 2970
Size: 4775
SacI G_AGCT'C
Cuts at: 1789 1789
Size: 4775
SalI G'TCGA_C
Cuts at: 1812 1812
Size: 4775
SanDI GG'GwC_CC
Cuts at: 1017 1017
Size: 4775
SapI GCTCTTCn'nnn_
Cuts at: 460 485 2798 3008 460
Size: 25 2313 210 2227
Fragments arranged by size:
2313 2227 210 25
SbfI CC_TGCA'GG
Cuts at: 1822 1822
Size: 4775
ScaI AGT'ACT
Cuts at: 220 4292 220
Size: 4072 703
SfcI C'TryA_G
Cuts at: 33 222 250 259 442 1659 1818 2503 3496
Size: 189 28 9 183 1217 159 685 993
Cuts at: 3496 3687 33
Size: 191 1121
Fragments arranged by size:
1217 1121 993 685 191 189 183 159
28 9
SfoI GGC'GCC
Cuts at: 123 2207 2455 123
Size: 2084 248 2443
Fragments arranged by size:
2443 2084 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4775
SmaI CCC'GGG
Cuts at: 975 1797 975
Size: 822 3953
SmlI C'TyrA_G
Cuts at: 352 1930 2845 3337 3599 352
Size: 1578 915 492 262 1528
Fragments arranged by size:
1578 1528 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4775
SphI G_CATG'C
Cuts at: 1107 1828 2328 1107
Size: 721 500 3554
Fragments arranged by size:
3554 721 500
SspI AAT'ATT
Cuts at: 603 603
Size: 4775
StuI AGG'CCT
Cuts at: 505 1649 505
Size: 1144 3631
StyI C'CwwG_G
Cuts at: 479 749 1062 1416 1709 1909 2263 479
Size: 270 313 354 293 200 354 2991
Fragments arranged by size:
2991 354 354 313 293 270 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 882 2358 2491 2950 3159 882
Size: 1476 133 459 209 2498
Fragments arranged by size:
2498 1476 459 209 133
TatI w'GTAC_w
Cuts at: 218 4290 218
Size: 4072 703
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 277 309 825 857 4292 4324 277
Size: 32 516 32 3435 32 728
Fragments arranged by size:
3435 728 516 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2572 2572
Size: 4775
XbaI T'CTAG_A
Cuts at: 1806 1806
Size: 4775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 651 756 1623 2270 150
Size: 501 105 867 647 2655
Fragments arranged by size:
2655 867 647 501 105
XmaI C'CCGG_G
Cuts at: 973 1795 973
Size: 822 3953
XmnI GAAnn'nnTTC
Cuts at: 604 604
Size: 4775
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI