Restriction map for
pF1KB9974
(Circular) (Six-base) MAPSORT of: pF1KB9974.seq Check: 7713 from: 1 to: 5234
pF1KB9974 5234 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 2099 2099
Size: 5234
AccI GT'mk_AC
Cuts at: 2272 2272
Size: 5234
Acc65I G'GTAC_C
Cuts at: 2250 2250
Size: 5234
AclI AA'CG_TT
Cuts at: 5074 5074
Size: 5234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 3059 3491 4238 4506 400
Size: 2659 432 747 268 1128
Fragments arranged by size:
2659 1128 747 432 268
AflIII A'CryG_T
Cuts at: 2586 3690 4667 2586
Size: 1104 977 3153
Fragments arranged by size:
3153 1104 977
AgeI A'CCGG_T
Cuts at: 355 905 3622 355
Size: 550 2717 1967
Fragments arranged by size:
2717 1967 550
AleI CACnn'nnGTG
Cuts at: 190 274 580 1624 190
Size: 84 306 1044 3800
Fragments arranged by size:
3800 1044 306 84
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2050 2082 2050
Size: 32 5202
AlwNI CAG_nnn'CTG
Cuts at: 126 2539 4106 126
Size: 2413 1567 1254
Fragments arranged by size:
2413 1567 1254
ApaI G_GGCC'C
Cuts at: 1394 1765 1900 1956 1394
Size: 371 135 56 4672
Fragments arranged by size:
4672 371 135 56
ApaLI G'TGCA_C
Cuts at: 4004 4004
Size: 5234
ApoI r'AATT_y
Cuts at: 2238 4587 4843 2238
Size: 2349 256 2629
Fragments arranged by size:
2629 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5234
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5234
AvaI C'yCGr_G
Cuts at: 272 349 941 1100 1899 2071 2254 272
Size: 77 592 159 799 172 183 3252
Fragments arranged by size:
3252 799 592 183 172 159 77
BamHI G'GATC_C
Cuts at: 479 2034 2259 479
Size: 1555 225 3454
Fragments arranged by size:
3454 1555 225
BanI G'GyrC_C
Cuts at: 455 1214 1427 1859 1997 2076 2250 2664 2912
Size: 759 213 432 138 79 174 414 248
Cuts at: 2912 2947 455
Size: 35 2742
Fragments arranged by size:
2742 759 432 414 248 213 174 138
79 35
BanII G_rGCy'C
Cuts at: 668 1394 1765 1900 1956 2248 3278 668
Size: 726 371 135 56 292 1030 2624
Fragments arranged by size:
2624 1030 726 371 292 135 56
BbeI G_GCGC'C
Cuts at: 1218 1863 2001 2668 2916 1218
Size: 645 138 667 248 3536
Fragments arranged by size:
3536 667 645 248 138
BbsI GAAGACnn'nnnn_
Cuts at: 503 1143 2091 503
Size: 640 948 3646
Fragments arranged by size:
3646 948 640
BbvCI CC'TCA_GC
Cuts at: 1575 1575
Size: 5234
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 511 545 735 769 511
Size: 34 190 34 4976
Fragments arranged by size:
4976 190 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 653 1276 3132 3893 5234 653
Size: 623 1856 761 1341 653
Fragments arranged by size:
1856 1341 761 653 623
BclI T'GATC_A
Cuts at: 2754 2754
Size: 5234
BglI GCCn_nnn'nGGC
Cuts at: 1936 1936
Size: 5234
BglII A'GATC_T
Cuts at: 2749 4786 2749
Size: 2037 3197
BlpI GC'TnA_GC
Cuts at: 2346 2346
Size: 5234
Bme1580I G_kGCm'C
Cuts at: 1394 1765 1900 1956 2221 2859 2952 4008 1394
Size: 371 135 56 265 638 93 1056 2620
Fragments arranged by size:
2620 1056 638 371 265 135 93 56
BmgBI CAC'GTC
Cuts at: 1413 1935 2016 1413
Size: 522 81 4631
Fragments arranged by size:
4631 522 81
BmrI ACTGGGnnnn_n'
Cuts at: 2446 2527 2862 5006 2446
Size: 81 335 2144 2674
Fragments arranged by size:
2674 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 840 1848 3601 840
Size: 1008 1753 2473
Fragments arranged by size:
2473 1753 1008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 313 2025 224
Size: 89 1712 3433
Fragments arranged by size:
3433 1712 89
Bpu10I CC'TnA_GC
Cuts at: 1454 1575 1454
Size: 121 5113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 2410 3289 3781 4079 433
Size: 1977 879 492 298 1588
Fragments arranged by size:
1977 1588 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 200 346 4938 200
Size: 146 4592 496
Fragments arranged by size:
4592 496 146
BsaAI yAC'GTr
Cuts at: 2196 2587 2196
Size: 391 4843
BsaBI GATnn'nnATC
Cuts at: 6 2748 4785 6
Size: 2742 2037 455
Fragments arranged by size:
2742 2037 455
BsaHI Gr'CG_yC
Cuts at: 524 562 710 752 1215 1673 1860 1905 1998
Size: 38 148 42 463 458 187 45 93
Cuts at: 1998 2096 2665 2913 3615 5111 524
Size: 98 569 248 702 1496 647
Fragments arranged by size:
1496 702 647 569 463 458 248 187
148 98 93 45 42 38
BsaWI w'CCGG_w
Cuts at: 355 905 1516 1638 1958 2060 2423 2944 3622
Size: 550 611 122 320 102 363 521 678
Cuts at: 3622 3896 4043 4454 355
Size: 274 147 411 1135
Fragments arranged by size:
1135 678 611 550 521 411 363 320
274 147 122 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 1012 1042 1919 1949 838
Size: 30 144 30 877 30 4123
Fragments arranged by size:
4123 877 144 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1837 1837
Size: 5234
BseYI C'CCAG_C
Cuts at: 147 922 1326 1893 1917 2659 3417 3994 4490
Size: 775 404 567 24 742 758 577 496
Cuts at: 4490 147
Size: 891
Fragments arranged by size:
891 775 758 742 577 567 496 404
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 822 894 996 822
Size: 72 102 5060
Fragments arranged by size:
5060 102 72
BsiEI CG_ry'CG
Cuts at: 104 852 906 1026 1071 1756 2776 2822 4030
Size: 748 54 120 45 685 1020 46 1208
Cuts at: 4030 104
Size: 1308
Fragments arranged by size:
1308 1208 1020 748 685 120 54 46
45
BsiHKAI G_wGCw'C
Cuts at: 140 197 632 2248 3026 3216 4008 140
Size: 57 435 1616 778 190 792 1366
Fragments arranged by size:
1616 1366 792 778 435 190 57
BsmI GAATG_Cn'
Cuts at: 698 698
Size: 5234
BsmBI CGTCTCn'nnnn_
Cuts at: 629 701 629
Size: 72 5162
Bsp1286I G_dGCh'C
Cuts at: 140 197 632 668 1394 1765 1900 1956 2221
Size: 57 435 36 726 371 135 56 265
Cuts at: 2221 2248 2859 2952 3026 3216 3278 4008 140
Size: 27 611 93 74 190 62 730 1366
Fragments arranged by size:
1366 730 726 611 435 371 265 190
135 93 74 62 57 56 36 27
BspEI T'CCGG_A
Cuts at: 1958 4454 1958
Size: 2496 2738
BspHI T'CATG_A
Cuts at: 4410 4410
Size: 5234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2284 2498 2800 3181 2284
Size: 214 302 381 4337
Fragments arranged by size:
4337 381 302 214
BsrBI CCG'CTC
Cuts at: 50 567 824 1830 3526 3580 5064 5229 50
Size: 517 257 1006 1696 54 1484 165 55
Fragments arranged by size:
1696 1484 1006 517 257 165 55 54
BsrDI GCAATG_nn'
Cuts at: 957 3146 957
Size: 2189 3045
BsrFI r'CCGG_y
Cuts at: 355 905 1751 3232 3413 3622 355
Size: 550 846 1481 181 209 1967
Fragments arranged by size:
1967 1481 846 550 209 181
BsrGI T'GTAC_A
Cuts at: 584 1874 584
Size: 1290 3944
BssSI C'ACGA_G
Cuts at: 1297 3505 3863 1297
Size: 2208 358 2668
Fragments arranged by size:
2668 2208 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2611 2611
Size: 5234
BstBI TT'CG_AA
Cuts at: 3595 3595
Size: 5234
BstEII G'GTnAC_C
Cuts at: 1944 1944
Size: 5234
BstXI CCAn_nnnn'nTGG
Cuts at: 2055 2055
Size: 5234
BstYI r'GATC_y
Cuts at: 1 479 2034 2259 2726 2749 3084 3330 4342
Size: 478 1555 225 467 23 335 246 1012
Cuts at: 4342 4428 4440 4786 1
Size: 86 12 346 449
Fragments arranged by size:
1555 1012 478 467 449 346 335 246
225 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2222 2222
Size: 5234
BtgI C'CryG_G
Cuts at: 397 505 881 1016 1203 1459 1490 397
Size: 108 376 135 187 256 31 4141
Fragments arranged by size:
4141 376 256 187 135 108 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1662 3184 3365 3528 1662
Size: 1522 181 163 3368
Fragments arranged by size:
3368 1522 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 2221 2477 1598
Size: 623 256 4355
Fragments arranged by size:
4355 623 256
DraI TTT'AAA
Cuts at: 2233 2233
Size: 5234
DrdI GACnn_nn'nnGTC
Cuts at: 2940 3798 2940
Size: 858 4376
EaeI y'GGCC_r
Cuts at: 884 1317 1616 1753 2819 2993 3384 3411 4756
Size: 433 299 137 1066 174 391 27 1345
Cuts at: 4756 884
Size: 1362
Fragments arranged by size:
1362 1345 1066 433 391 299 174 137
27
EagI C'GGCC_G
Cuts at: 1753 2819 1753
Size: 1066 4168
EarI CTCTTCn'nnn_
Cuts at: 202 1087 3257 3467 202
Size: 885 2170 210 1969
Fragments arranged by size:
2170 1969 885 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 996 3442 3752 3898 5044 996
Size: 2446 310 146 1146 1186
Fragments arranged by size:
2446 1186 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2246 2246
Size: 5234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 313 400 2025 3059 3491 4238 4506 224
Size: 89 87 1625 1034 432 747 268 952
Fragments arranged by size:
1625 1034 952 747 432 268 89 87
EcoNI CCTnn'n_nnAGG
Cuts at: 745 1171 745
Size: 426 4808
EcoO109I rG'GnC_Cy
Cuts at: 310 408 1390 1391 1567 1591 1761 1762 1952
Size: 98 982 1 176 24 170 1 190
Cuts at: 1952 1953 2373 310
Size: 1 420 3171
Fragments arranged by size:
3171 982 420 190 176 170 98 24
1 1 1
EcoRI G'AATT_C
Cuts at: 2238 2238
Size: 5234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1281 1863 3253 1281
Size: 582 1390 3262
Fragments arranged by size:
3262 1390 582
FspI TGC'GCA
Cuts at: 3015 3015
Size: 5234
HaeII r_GCGC'y
Cuts at: 180 374 1218 1360 1548 1863 2001 2048 2668
Size: 194 844 142 188 315 138 47 620
Cuts at: 2668 2916 3938 4917 180
Size: 248 1022 979 497
Fragments arranged by size:
1022 979 844 620 497 315 248 194
188 142 138 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 204 236 286 318 721 753 838 870 1012
Size: 32 50 32 403 32 85 32 142
Cuts at: 1012 1044 1327 1359 2019 2051 3076 3106 3108
Size: 32 283 32 660 32 1025 30 2
Cuts at: 3108 3138 204
Size: 30 2300
Fragments arranged by size:
2300 1025 660 403 283 142 85 50
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2273 2273
Size: 5234
HindIII A'AGCT_T
Cuts at: 1840 1840
Size: 5234
KasI G'GCGC_C
Cuts at: 1214 1859 1997 2664 2912 1214
Size: 645 138 667 248 3536
Fragments arranged by size:
3536 667 645 248 138
KpnI G_GTAC'C
Cuts at: 2254 2254
Size: 5234
MluI A'CGCG_T
Cuts at: 4667 4667
Size: 5234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 787 881 1055 1492 3905 4089 787
Size: 94 174 437 2413 184 1932
Fragments arranged by size:
2413 1932 437 184 174 94
MscI TGG'CCA
Cuts at: 2995 2995
Size: 5234
MslI CAynn'nnrTG
Cuts at: 190 274 580 1433 1624 3223 3350 190
Size: 84 306 853 191 1599 127 2074
Fragments arranged by size:
2074 1599 853 306 191 127 84
MspA1I CmG'CkG
Cuts at: 237 507 1043 1205 1555 1579 1698 1991 2345
Size: 270 536 162 350 24 119 293 354
Cuts at: 2345 2659 3019 4032 4277 237
Size: 314 360 1013 245 1194
Fragments arranged by size:
1194 1013 536 360 354 350 314 293
270 245 162 119 24
NaeI GCC'GGC
Cuts at: 1753 3415 1753
Size: 1662 3572
NarI GG'CG_CC
Cuts at: 1215 1860 1998 2665 2913 1215
Size: 645 138 667 248 3536
Fragments arranged by size:
3536 667 645 248 138
NgoMIV G'CCGG_C
Cuts at: 1751 3413 1751
Size: 1662 3572
NspI r_CATG'y
Cuts at: 2287 2787 3694 2287
Size: 500 907 3827
Fragments arranged by size:
3827 907 500
PasI CC'CwG_GG
Cuts at: 741 741
Size: 5234
PciI A'CATG_T
Cuts at: 3690 3690
Size: 5234
PflMI CCAn_nnn'nTGG
Cuts at: 1326 1326
Size: 5234
PfoI T'CCnGG_A
Cuts at: 2158 2158
Size: 5234
PmeI GTTT'AAAC
Cuts at: 2233 2233
Size: 5234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4373 4405 4373
Size: 32 5202
PpuMI rG'GwC_Cy
Cuts at: 408 1567 1591 408
Size: 1159 24 4051
Fragments arranged by size:
4051 1159 24
PspOMI G'GGCC_C
Cuts at: 1390 1761 1896 1952 1390
Size: 371 135 56 4672
Fragments arranged by size:
4672 371 135 56
PstI C_TGCA'G
Cuts at: 2216 2281 2966 2216
Size: 65 685 4484
Fragments arranged by size:
4484 685 65
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5234
PvuII CAG'CTG
Cuts at: 237 1555 1579 1698 2659 3019 237
Size: 1318 24 119 961 360 2452
Fragments arranged by size:
2452 1318 961 360 119 24
RsrII CG'GwC_CG
Cuts at: 3429 3429
Size: 5234
SacI G_AGCT'C
Cuts at: 2248 2248
Size: 5234
SacII CC_GC'GG
Cuts at: 508 1206 508
Size: 698 4536
SalI G'TCGA_C
Cuts at: 2271 2271
Size: 5234
SanDI GG'GwC_CC
Cuts at: 408 408
Size: 5234
SapI GCTCTTCn'nnn_
Cuts at: 202 1087 3257 3467 202
Size: 885 2170 210 1969
Fragments arranged by size:
2170 1969 885 210
SbfI CC_TGCA'GG
Cuts at: 2281 2281
Size: 5234
ScaI AGT'ACT
Cuts at: 1180 1723 4751 1180
Size: 543 3028 1663
Fragments arranged by size:
3028 1663 543
SexAI A'CCwGG_T
Cuts at: 2174 2174
Size: 5234
SfcI C'TryA_G
Cuts at: 33 2212 2277 2962 3955 4146 33
Size: 2179 65 685 993 191 1121
Fragments arranged by size:
2179 1121 993 685 191 65
SfoI GGC'GCC
Cuts at: 1216 1861 1999 2666 2914 1216
Size: 645 138 667 248 3536
Fragments arranged by size:
3536 667 645 248 138
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5234
SmaI CCC'GGG
Cuts at: 943 2073 2256 943
Size: 1130 183 3921
Fragments arranged by size:
3921 1130 183
SmlI C'TyrA_G
Cuts at: 448 2389 3304 3796 4058 448
Size: 1941 915 492 262 1624
Fragments arranged by size:
1941 1624 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5234
SphI G_CATG'C
Cuts at: 2287 2787 2287
Size: 500 4734
StyI C'CwwG_G
Cuts at: 990 1278 2368 2722 990
Size: 288 1090 354 3502
Fragments arranged by size:
3502 1090 354 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1803 1860 2817 2950 3409 3618 1803
Size: 57 957 133 459 209 3419
Fragments arranged by size:
3419 957 459 209 133 57
TatI w'GTAC_w
Cuts at: 584 1178 1721 1874 4749 584
Size: 594 543 153 2875 1069
Fragments arranged by size:
2875 1069 594 543 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1919 1951 4751 4783 1919
Size: 32 2800 32 2370
Fragments arranged by size:
2800 2370 32 32
Tth111I GACn'n_nGTC
Cuts at: 604 1813 3031 604
Size: 1209 1218 2807
Fragments arranged by size:
2807 1218 1209
XbaI T'CTAG_A
Cuts at: 2265 2265
Size: 5234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2729 2729
Size: 5234
XmaI C'CCGG_G
Cuts at: 941 2071 2254 941
Size: 1130 183 3921
Fragments arranged by size:
3921 1130 183
XmnI GAAnn'nnTTC
Cuts at: 1666 1843 1666
Size: 177 5057
ZraI GAC'GTC
Cuts at: 2097 2097
Size: 5234
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AscI AvrII BaeI
BfrBI BmtI BsiWI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI